##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660657_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23433946 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40998750274495 34.0 31.0 34.0 31.0 34.0 2 32.56224636687308 34.0 31.0 34.0 31.0 34.0 3 32.58503074983616 34.0 31.0 34.0 31.0 34.0 4 35.936881564888814 37.0 37.0 37.0 35.0 37.0 5 35.93089418231142 37.0 37.0 37.0 35.0 37.0 6 35.94570218775788 37.0 37.0 37.0 35.0 37.0 7 35.890621408788775 37.0 36.0 37.0 35.0 37.0 8 35.91891813696251 37.0 37.0 37.0 35.0 37.0 9 37.653960242120554 39.0 38.0 39.0 35.0 39.0 10-11 37.57464223054879 39.0 38.0 39.0 35.0 39.0 12-13 37.644166693052895 39.0 38.0 39.0 35.0 39.0 14-15 39.13627491503138 41.0 39.0 41.0 36.0 41.0 16-17 39.09657494303349 41.0 39.0 41.0 36.0 41.0 18-19 39.05832498291154 41.0 39.0 41.0 36.0 41.0 20-21 38.984240917001344 41.0 39.0 41.0 35.5 41.0 22-23 38.834218680029394 40.5 39.0 41.0 34.5 41.0 24-25 38.83499669667243 40.0 39.0 41.0 35.0 41.0 26-27 38.812097480296316 40.0 39.0 41.0 35.0 41.0 28-29 38.73834664891692 40.0 39.0 41.0 35.0 41.0 30-31 38.64450912364482 40.0 38.0 41.0 35.0 41.0 32-33 38.53694484488443 40.0 38.0 41.0 34.0 41.0 34-35 38.43066078158583 40.0 38.0 41.0 34.0 41.0 36-37 38.266330369627035 40.0 38.0 41.0 34.0 41.0 38-39 37.89075841089674 40.0 37.0 41.0 33.0 41.0 40-41 37.747075780579166 40.0 37.0 41.0 33.0 41.0 42-43 37.59057881246291 40.0 36.5 41.0 33.0 41.0 44-45 37.44712533689375 40.0 36.0 41.0 32.5 41.0 46-47 37.2751693632818 40.0 36.0 41.0 32.0 41.0 48-49 37.282102723971455 40.0 36.0 41.0 32.0 41.0 50-51 36.30383896933107 38.5 35.0 40.0 31.0 40.5 52-53 36.46638760710637 38.5 35.0 39.5 31.0 40.5 54-55 36.826890166086415 40.0 36.0 41.0 30.5 41.0 56-57 36.505828681179004 40.0 35.0 41.0 29.5 41.0 58-59 36.26547549866335 39.0 35.0 41.0 29.0 41.0 60-61 36.03190444323803 39.0 35.0 41.0 28.0 41.0 62-63 35.754521240255485 38.5 35.0 41.0 28.0 41.0 64-65 35.45780495098862 37.5 35.0 40.5 28.0 41.0 66-67 35.15111262098155 37.0 35.0 40.0 28.0 41.0 68-69 34.85391617357145 36.5 35.0 39.5 27.5 41.0 70-71 34.526731434816824 36.0 34.0 39.0 27.0 41.0 72-73 34.17566866459451 35.0 34.0 39.0 26.0 41.0 74-75 33.818427997572414 35.0 34.0 37.5 26.0 40.0 76-77 33.462302785028186 35.0 34.0 37.0 26.0 39.0 78-79 33.12219491331081 35.0 34.0 36.5 25.5 39.0 80-81 32.808990897222344 35.0 33.5 36.0 25.0 38.0 82-83 32.54579141302109 35.0 33.0 36.0 24.5 37.0 84-85 32.312381342007015 35.0 33.0 35.0 24.0 37.0 86-87 32.11492332533326 35.0 33.0 35.0 24.0 36.5 88-89 31.932445158830696 35.0 33.0 35.0 23.0 36.0 90-91 31.80241490272274 35.0 33.0 35.0 23.0 36.0 92-93 31.674584809574966 35.0 33.0 35.0 23.0 36.0 94-95 31.568421425909236 35.0 33.0 35.0 21.5 35.0 96-97 31.46326546967378 35.0 33.0 35.0 20.0 35.0 98-99 31.21387104416815 35.0 32.0 35.0 19.5 35.0 100 31.141603765750762 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48919277044729625 1101 2 0.4644659192597018 1101 3 0.4906320124007095 1101 4 0.4005867530879783 1101 5 0.4033618930896381 1101 6 0.3711646984506558 1101 7 0.39314805905423356 1101 8 0.3975202812414409 1101 9 0.5079842242901407 1101 10-11 0.47967137518920566 1101 12-13 0.48381309499738023 1101 14-15 0.6112855644294442 1101 16-17 0.623807938265486 1101 18-19 0.5994789440184078 1101 20-21 0.608035706341731 1101 22-23 0.6267816362975225 1101 24-25 0.6507330978269934 1101 26-27 0.6240687893593346 1101 28-29 0.6315853377144123 1101 30-31 0.6140968162318075 1101 32-33 0.6531106505614162 1101 34-35 0.6296735076026891 1101 36-37 0.6498532414413418 1101 38-39 0.7056368764446006 1101 40-41 0.6837630937974879 1101 42-43 0.687104060133116 1101 44-45 0.6685138495443326 1101 46-47 0.697704148198234 1101 48-49 0.6907891305349096 1101 50-51 0.6432909531888242 1101 52-53 0.7441220390525416 1101 54-55 0.8246003492938812 1101 56-57 0.7605137070741748 1101 58-59 0.7108505420482629 1101 60-61 0.6999231599795763 1101 62-63 0.6929850959544765 1101 64-65 0.6852886513973857 1101 66-67 0.6624367107607156 1101 68-69 0.6080836517878936 1101 70-71 0.583958861062662 1101 72-73 0.5899898525129075 1101 74-75 0.5899030918920616 1101 76-77 0.5673186861680435 1101 78-79 0.5905454016029381 1101 80-81 0.5959221085580637 1101 82-83 0.5616607431929737 1101 84-85 0.5481302664999745 1101 86-87 0.6059476669011943 1101 88-89 0.5767694659052829 1101 90-91 0.586144515815139 1101 92-93 0.5471278372008577 1101 94-95 0.5343066666999299 1101 96-97 0.520723833874996 1101 98-99 0.35178577028901614 1101 100 0.403725161685653 1102 1 0.45775242698287144 1102 2 0.4295021280792355 1102 3 0.4553967405075312 1102 4 0.39664411032919844 1102 5 0.3623917765781073 1102 6 0.3253728057951051 1102 7 0.3700031626876523 1102 8 0.3946745816278536 1102 9 0.45469061064078176 1102 10-11 0.5139308079651244 1102 12-13 0.4836261870964691 1102 14-15 0.5481546823804493 1102 16-17 0.5727142243844412 1102 18-19 0.5747591522214393 1102 20-21 0.56136866721031 1102 22-23 0.5862127008860369 1102 24-25 0.6012189682996478 1102 26-27 0.5697201547181692 1102 28-29 0.5846204724791022 1102 30-31 0.585816886556124 1102 32-33 0.6037685674322546 1102 34-35 0.5881250905487576 1102 36-37 0.6321191506535015 1102 38-39 0.6791334791474597 1102 40-41 0.659876672024005 1102 42-43 0.7003441755516278 1102 44-45 0.6517054634818109 1102 46-47 0.6949986129533983 1102 48-49 0.6573596769226953 1102 50-51 0.6756997050191558 1102 52-53 0.7179528003922258 1102 54-55 0.7788198610535204 1102 56-57 0.7628264059129961 1102 58-59 0.7342464812499401 1102 60-61 0.7124518968541054 1102 62-63 0.7192191980539988 1102 64-65 0.7098771930781282 1102 66-67 0.6897700209938193 1102 68-69 0.6990446977494571 1102 70-71 0.7000454761257373 1102 72-73 0.6790834354219939 1102 74-75 0.6398487083289197 1102 76-77 0.6471636195002901 1102 78-79 0.62486250548654 1102 80-81 0.6256138809917431 1102 82-83 0.5784917923848667 1102 84-85 0.6309103996007579 1102 86-87 0.61591805303906 1102 88-89 0.6133409352427286 1102 90-91 0.6056563973083975 1102 92-93 0.5614215960743785 1102 94-95 0.5983600083154421 1102 96-97 0.5785265625528666 1102 98-99 0.42859359262110175 1102 100 0.46561026038801856 1103 1 0.39196543063691536 1103 2 0.3879477212436697 1103 3 0.38897894637344166 1103 4 0.3536971675810747 1103 5 0.37730885193442276 1103 6 0.33008550633141454 1103 7 0.3627938275686162 1103 8 0.37935849658745724 1103 9 0.4563023866036531 1103 10-11 0.45874766081580276 1103 12-13 0.41263779215761787 1103 14-15 0.5092775481809966 1103 16-17 0.5634670295193303 1103 18-19 0.5608673620523632 1103 20-21 0.5360466825524597 1103 22-23 0.5381631087449321 1103 24-25 0.5561552303915391 1103 26-27 0.5470180633269237 1103 28-29 0.5784500950757518 1103 30-31 0.5629095812428915 1103 32-33 0.5825168966593068 1103 34-35 0.5893487682964462 1103 36-37 0.5726247959798059 1103 38-39 0.6153824471980727 1103 40-41 0.62648322156398 1103 42-43 0.6163272415371708 1103 44-45 0.6001128141560841 1103 46-47 0.610527751536587 1103 48-49 0.5567305558211615 1103 50-51 0.4860154478128038 1103 52-53 0.558178847931039 1103 54-55 0.6123443320533397 1103 56-57 0.6255672561315109 1103 58-59 0.5950358246048921 1103 60-61 0.568921780666173 1103 62-63 0.5220753626917016 1103 64-65 0.4851824718354294 1103 66-67 0.4811606311554115 1103 68-69 0.4302446595400795 1103 70-71 0.47668854784507175 1103 72-73 0.43765570160901035 1103 74-75 0.4366562802369458 1103 76-77 0.43932996546406855 1103 78-79 0.4339698837101693 1103 80-81 0.42297518217294083 1103 82-83 0.41465778991864255 1103 84-85 0.4107295487371587 1103 86-87 0.3989640394639977 1103 88-89 0.4145744219321834 1103 90-91 0.4211857575350315 1103 92-93 0.4038555401903885 1103 94-95 0.39694251881915577 1103 96-97 0.3987847900104029 1103 98-99 0.28678801282160293 1103 100 0.35668964223734534 1104 1 0.3275426866159634 1104 2 0.32508867748764203 1104 3 0.32111942419595607 1104 4 0.29396119104856666 1104 5 0.29988700922871914 1104 6 0.2844043794788931 1104 7 0.2755943877708802 1104 8 0.30617494161859327 1104 9 0.3570880322437233 1104 10-11 0.3702895314143362 1104 12-13 0.34204435884652895 1104 14-15 0.45358325992029336 1104 16-17 0.45626605251982255 1104 18-19 0.4294930207663441 1104 20-21 0.4409280194840832 1104 22-23 0.4491222306742557 1104 24-25 0.4568307519577459 1104 26-27 0.4460845532076547 1104 28-29 0.42202370568205083 1104 30-31 0.432052153785186 1104 32-33 0.43095470345249964 1104 34-35 0.41390172722498164 1104 36-37 0.4085691745075479 1104 38-39 0.4057251893636291 1104 40-41 0.41104580058215134 1104 42-43 0.41566092862089477 1104 44-45 0.42271048454317395 1104 46-47 0.4088812711585561 1104 48-49 0.378624100083087 1104 50-51 0.2883690816465716 1104 52-53 0.3794792054415268 1104 54-55 0.45229347100480055 1104 56-57 0.3972605910448834 1104 58-59 0.39594104830668186 1104 60-61 0.36916316826784623 1104 62-63 0.36627814045424856 1104 64-65 0.3547508415894711 1104 66-67 0.3353014879402778 1104 68-69 0.37126754642101645 1104 70-71 0.36437841694115036 1104 72-73 0.33849314123003893 1104 74-75 0.29588306859302094 1104 76-77 0.2890849739627157 1104 78-79 0.31817009983544153 1104 80-81 0.31771638035203864 1104 82-83 0.3114798689710483 1104 84-85 0.3420824830411391 1104 86-87 0.34211710543417695 1104 88-89 0.32294076686930495 1104 90-91 0.3573934534855603 1104 92-93 0.33092396648559586 1104 94-95 0.30528346729010636 1104 96-97 0.2773821507630636 1104 98-99 0.21262633487384264 1104 100 0.2702174175079328 1105 1 0.3006759192068813 1105 2 0.3015614197997465 1105 3 0.2969527134220016 1105 4 0.28935700573887857 1105 5 0.2879386460740747 1105 6 0.2663706057428499 1105 7 0.2781722501252375 1105 8 0.2902222180654306 1105 9 0.33696400392041426 1105 10-11 0.3096796773951027 1105 12-13 0.32486517234355006 1105 14-15 0.41974112338792935 1105 16-17 0.39200220382326734 1105 18-19 0.4215897437385294 1105 20-21 0.411482928132763 1105 22-23 0.4437297318945568 1105 24-25 0.4381470282851865 1105 26-27 0.4123615952485338 1105 28-29 0.3935208075255687 1105 30-31 0.4163480961746444 1105 32-33 0.4173288307607663 1105 34-35 0.4335275654514845 1105 36-37 0.4316740792788849 1105 38-39 0.4578877251948157 1105 40-41 0.4659810367243651 1105 42-43 0.45505239440108625 1105 44-45 0.46624391178058033 1105 46-47 0.4797425168823679 1105 48-49 0.4466359159123954 1105 50-51 0.41263525606224505 1105 52-53 0.4870400150297556 1105 54-55 0.537985080786747 1105 56-57 0.47171797909751234 1105 58-59 0.48923973929814224 1105 60-61 0.44969560681818876 1105 62-63 0.45983736190661517 1105 64-65 0.4634830894322093 1105 66-67 0.4594093889982602 1105 68-69 0.44029523171025886 1105 70-71 0.39228407165946066 1105 72-73 0.41297019292665027 1105 74-75 0.40521239917296725 1105 76-77 0.40967876882649534 1105 78-79 0.41894318774352257 1105 80-81 0.43245047427279815 1105 82-83 0.37618815841933895 1105 84-85 0.391058486215222 1105 86-87 0.3753531292026011 1105 88-89 0.4008260980439182 1105 90-91 0.4193471744788617 1105 92-93 0.3916558164737758 1105 94-95 0.40155242504653543 1105 96-97 0.37832124960947766 1105 98-99 0.26179034792303213 1105 100 0.2598963670624599 1106 1 0.36192938069522995 1106 2 0.3354483379334354 1106 3 0.34780179678423195 1106 4 0.28949366324820147 1106 5 0.32650919323085503 1106 6 0.3127330973624751 1106 7 0.3164141644125351 1106 8 0.31793733605692864 1106 9 0.4020596015601754 1106 10-11 0.3969693244917778 1106 12-13 0.3991336627163875 1106 14-15 0.46947954542819303 1106 16-17 0.47837386760430434 1106 18-19 0.47388215553277746 1106 20-21 0.507351676983788 1106 22-23 0.5088490593770914 1106 24-25 0.45895337068494513 1106 26-27 0.4571082277331371 1106 28-29 0.5087563918791034 1106 30-31 0.49493814964935723 1106 32-33 0.4918755044864014 1106 34-35 0.49682390852713354 1106 36-37 0.5310953067644562 1106 38-39 0.5132556503246661 1106 40-41 0.5043456464158922 1106 42-43 0.5374608398447336 1106 44-45 0.5189999146970834 1106 46-47 0.5695788285272627 1106 48-49 0.5637326020323243 1106 50-51 0.5306353969738637 1106 52-53 0.49915674664099896 1106 54-55 0.5403021114862625 1106 56-57 0.5500849089553554 1106 58-59 0.5389856657906478 1106 60-61 0.5117672830365407 1106 62-63 0.5061790465959177 1106 64-65 0.483363824566446 1106 66-67 0.46957263043217523 1106 68-69 0.48307613984840003 1106 70-71 0.4886106231667213 1106 72-73 0.494818039058039 1106 74-75 0.5068698243695948 1106 76-77 0.45836665598095294 1106 78-79 0.455509654199048 1106 80-81 0.4784506657558225 1106 82-83 0.47307766812240715 1106 84-85 0.5062586944852825 1106 86-87 0.46797741416452965 1106 88-89 0.4718136753553104 1106 90-91 0.4704554267282681 1106 92-93 0.4876760620519569 1106 94-95 0.5078138316944525 1106 96-97 0.5243679161174484 1106 98-99 0.439596408403947 1106 100 0.453985411164453 1107 1 0.28858472007793523 1107 2 0.2552977677591244 1107 3 0.24814569875945836 1107 4 0.27498415562163103 1107 5 0.27656475884273846 1107 6 0.21603970248035154 1107 7 0.2165997601086005 1107 8 0.25742629839462694 1107 9 0.3212269381929289 1107 10-11 0.3514850314585445 1107 12-13 0.29773095274908457 1107 14-15 0.34009053351028484 1107 16-17 0.35161923195917666 1107 18-19 0.39125461294354125 1107 20-21 0.3543015213681002 1107 22-23 0.37400525144005314 1107 24-25 0.36434799727211953 1107 26-27 0.3255644225249412 1107 28-29 0.3436731811786089 1107 30-31 0.34247272626555514 1107 32-33 0.39373798291239837 1107 34-35 0.36672434036123036 1107 36-37 0.347016144443721 1107 38-39 0.36528096479249683 1107 40-41 0.35317959693021095 1107 42-43 0.3578712309476586 1107 44-45 0.39118817830369323 1107 46-47 0.37259547782787905 1107 48-49 0.3464554854340207 1107 50-51 0.22574570110887748 1107 52-53 0.28920039058062486 1107 54-55 0.4259435323541254 1107 56-57 0.40925550225009744 1107 58-59 0.3966347276952433 1107 60-61 0.4110577086621845 1107 62-63 0.4268841263235643 1107 64-65 0.3982340908099573 1107 66-67 0.3830819742104197 1107 68-69 0.38040409827534205 1107 70-71 0.35276842826855415 1107 72-73 0.3591067908883119 1107 74-75 0.3829353558909432 1107 76-77 0.37643918634855567 1107 78-79 0.3745931024325202 1107 80-81 0.36846730228521807 1107 82-83 0.3671827538688177 1107 84-85 0.3922015167173285 1107 86-87 0.3829025441911824 1107 88-89 0.37947135636440166 1107 90-91 0.39053851746557555 1107 92-93 0.42812406283855964 1107 94-95 0.4226722193269019 1107 96-97 0.3855027324088951 1107 98-99 0.2536543247841365 1107 100 0.2770637986387854 1108 1 0.12050945824205428 1108 2 0.1328664816957641 1108 3 0.12866391538874922 1108 4 0.13967897208845415 1108 5 0.11706408384843314 1108 6 0.09702537650004217 1108 7 0.0951277472872718 1108 8 0.11723351292817341 1108 9 0.14244852372289785 1108 10-11 0.1378215150821589 1108 12-13 0.12713536690682048 1108 14-15 0.14424193802233987 1108 16-17 0.1743400078172641 1108 18-19 0.1550637561141741 1108 20-21 0.17262748230144354 1108 22-23 0.18927483430544356 1108 24-25 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#Quality Count 2 658.0 3 220.0 4 216.0 5 201.0 6 662.0 7 5633.0 8 11769.0 9 15022.0 10 17024.0 11 16891.0 12 17139.0 13 18888.0 14 22279.0 15 29859.0 16 40679.0 17 53144.0 18 58626.0 19 64909.0 20 73558.0 21 89934.0 22 117939.0 23 176283.0 24 265832.0 25 350213.0 26 366738.0 27 317971.0 28 272623.0 29 268553.0 30 302511.0 31 365247.0 32 457893.0 33 597925.0 34 850082.0 35 1385085.0 36 2672035.0 37 5584331.0 38 6695005.0 39 1846472.0 40 3897.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.053838007599264 31.1990997008418 27.832579601426353 23.914482690132584 2 22.375256466788514 32.92767149844916 24.816600842894943 19.880471191867386 3 26.62028970117319 24.249010578118927 26.17987228202148 22.950827438686403 4 20.61170230938582 26.846857819510134 23.521888533425898 29.019551337678152 5 22.980784235780657 30.69932532015241 25.449281908280813 20.87060853578612 6 28.983795715193196 26.659422851705056 24.410104703334856 19.946676729766892 7 22.488800754376626 24.933228472466315 30.563900113833615 22.014070659323444 8 27.886480714472828 26.21219971440486 24.295617948708315 21.605701622413992 9 26.762157962881567 24.79801951229799 26.26878621834088 22.171036306479568 10-11 21.91937909427246 28.71607465623339 27.63729547284767 21.727250776646486 12-13 21.734930677039554 26.08334444245724 27.69566368161111 24.486061198892102 14-15 21.749614711661863 31.287346380265625 25.139960035257335 21.82307887281518 16-17 24.55443493363027 25.741148776575734 25.23305862350678 24.471357666287215 18-19 21.80610708001301 28.476111755829304 25.314584741909385 24.4031964222483 20-21 24.459342779322117 28.63737644411829 25.27087335124907 21.632407425310525 22-23 24.392134998181668 25.9961809162203 27.87688088937161 21.734803196226423 24-25 21.86553151351738 28.276130733962084 25.222395195907815 24.635942556612722 26-27 21.910398469335547 25.74650461330119 30.586616353001165 21.756480564362096 28-29 21.985350840246333 25.794277916450035 30.508922033511432 21.711449209792196 30-31 21.851210820869802 25.73766154581818 30.584914222860725 21.826213410451288 32-33 21.96196414329826 25.91605063714249 30.35748938984984 21.76449582970941 34-35 21.95335920860836 26.061068721836953 30.238865106937695 21.74670696261699 36-37 21.866791930398737 26.02209787603248 30.36451417424722 21.74659601932156 38-39 21.93541298066008 26.029202892577054 30.36659814301772 21.66878598374515 40-41 21.984678837625797 26.088177462654066 30.318071216817334 21.609072482902807 42-43 22.006014806843986 26.106180345818846 30.26122675552893 21.626578091808238 44-45 22.100468012994114 26.198733348265414 30.170933212083973 21.529865426656496 46-47 22.136072342479554 26.29311379736915 30.05072021259152 21.520093647559776 48-49 22.21419620242899 26.29034054871256 29.971586083028225 21.52387716583023 50-51 22.337849033426195 26.3568484371472 29.87002654444583 21.43527598498077 52-53 22.494157503450634 26.48416612957462 29.5836615266099 21.43801484036485 54-55 22.80894406394899 26.753780262787757 28.88728560462895 21.549990068634305 56-57 23.26322274637133 27.22535757411014 27.948791193930766 21.56262848558776 58-59 23.47351209655002 27.594191726713785 27.43425389037764 21.498042286358558 60-61 23.62591433876191 27.790274879662814 27.178725966868704 21.405084814706573 62-63 23.7486342068078 27.959400710201788 27.02211346009067 21.269851622899747 64-65 23.914955227163627 27.98685864091912 26.981275191797067 21.116910940120178 66-67 24.023086588857705 27.808367423359172 27.180369113444204 20.98817687433892 68-69 24.137732997758498 27.6853294652976 27.37777990436463 20.799157632579274 70-71 24.31318557593127 27.545951657864276 27.493543295765825 20.647319470438624 72-73 24.46135230568659 27.496803459243875 27.48912134500309 20.55272289006645 74-75 24.736011018974917 27.53754372512108 27.333736319053525 20.392708936850486 76-77 24.999772669824072 27.628407503236307 27.03561578664416 20.336204040295456 78-79 25.259402475281718 27.687331694255978 26.769875691205613 20.28339013925669 80-81 25.488762474048336 27.801838743509403 26.473415038144964 20.235983744297293 82-83 25.65397290512851 27.877737017160804 26.252119004609387 20.216171073101307 84-85 25.82412344754602 27.860483303955903 26.11797817802426 20.197415070473824 86-87 25.99077665655732 27.856892499064944 26.00788656760777 20.14444427676997 88-89 26.137099173559186 27.836767998306783 25.8992400516529 20.126892776481135 90-91 26.22588557329789 27.80513705406494 25.84944021252948 20.11953716010769 92-93 26.32650763068927 27.856777635097185 25.807367879415672 20.009346854797876 94-95 26.397466430738746 27.901490908754845 25.71876564162043 19.98227701888598 96-97 26.416141470621213 27.957493211576228 25.683294143771775 19.943071174030788 98-99 26.493747460051793 28.065115908537187 25.606076830700275 19.835059800710745 100 26.45751121589844 28.20138597802429 25.50167682895558 19.839425977121685 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 32837.0 1 22542.0 2 10281.0 3 7775.5 4 7149.0 5 7161.5 6 7346.5 7 7483.0 8 7408.0 9 7481.5 10 7954.0 11 12158.0 12 105149.5 13 123983.5 14 51954.0 15 52896.5 16 58227.0 17 63053.5 18 67807.0 19 72919.5 20 79005.0 21 87721.5 22 99265.0 23 112402.0 24 126410.0 25 142693.5 26 161363.0 27 184179.5 28 211596.0 29 239100.5 30 264846.0 31 291389.5 32 320643.5 33 352026.0 34 382734.5 35 415798.5 36 453669.5 37 486255.5 38 520265.0 39 556627.5 40 585625.0 41 614951.0 42 646819.5 43 674067.0 44 699841.0 45 734089.5 46 775574.5 47 817536.5 48 857581.0 49 888488.5 50 907324.5 51 917424.0 52 916222.5 53 903858.5 54 878227.0 55 841195.5 56 792767.5 57 726669.5 58 645708.0 59 555927.0 60 457009.5 61 360811.5 62 278359.0 63 207382.0 64 150755.5 65 108198.0 66 76383.5 67 54029.5 68 38334.5 69 27481.5 70 20459.0 71 15861.5 72 12827.0 73 9840.0 74 7523.0 75 6123.5 76 4660.0 77 3174.0 78 2250.5 79 1648.5 80 1156.5 81 884.0 82 686.0 83 533.5 84 437.0 85 357.5 86 288.5 87 249.0 88 213.0 89 187.0 90 163.5 91 123.5 92 104.5 93 93.5 94 82.5 95 74.0 96 58.0 97 44.0 98 28.5 99 17.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13632787239502897 2 0.0503969753962905 3 0.018584151384491543 4 0.07617155045078622 5 0.07976462862891295 6 0.08119844604916304 7 0.04763602339955891 8 0.04513964485537348 9 0.033272245314553514 10-11 0.02275758423271949 12-13 0.014959068353234236 14-15 0.011918607305828903 16-17 0.01685802297231546 18-19 0.016269133674712742 20-21 0.02294534603775224 22-23 0.019222114790227816 24-25 0.016732137216668504 26-27 0.015355928532053458 28-29 0.017075655973603422 30-31 0.015016677088869283 32-33 0.012140507620867609 34-35 0.009861762077970137 36-37 0.0076150213882032495 38-39 0.016367281890979864 40-41 0.024869904539337934 42-43 0.025298769571287738 44-45 0.024082585152325607 46-47 0.02605835141892023 48-49 0.022557020486434508 50-51 0.02268503989895684 52-53 0.01935440151650089 54-55 0.024099654407328582 56-57 0.030699055122854684 58-59 0.02333580524594535 60-61 0.023056296195271594 62-63 0.030225383296522062 64-65 0.03114072209605672 66-67 0.02363665086537282 68-69 0.024278881584859843 70-71 0.03539736756242419 72-73 0.04013408582575039 74-75 0.04107502850778951 76-77 0.04210558477859427 78-79 0.03657941347138036 80-81 0.0318320269236773 82-83 0.03476793878418939 84-85 0.0381327156766513 86-87 0.03809217619601923 88-89 0.03727711926962706 90-91 0.040245035983269735 92-93 0.03364350161086827 94-95 0.033560288992728754 96-97 0.03350908122771982 98-99 0.3135600807478177 100 0.14943279292356482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.3433946E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.408695319304535 #Duplication Level Percentage of deduplicated Percentage of total 1 77.96642746021492 40.86118741897062 2 11.316383660985094 11.861538068098477 3 4.040534977877106 6.352774997476622 4 1.99005043409278 4.171837874816729 5 1.1276527194777726 2.9549403900547886 6 0.7453625489949218 2.3438087239617036 7 0.5095250985990476 1.8692481955011262 8 0.36388233023419414 1.525647854185795 9 0.2773668928653902 1.3082793281859977 >10 1.5114299823095143 14.538452663683357 >50 0.12366206303731687 4.35877463590175 >100 0.026740287258416363 2.342876222843487 >500 4.383188554831042E-4 0.1490691338480593 >1k 4.7844128483264827E-4 0.4600724148005402 >5k 3.239195662895599E-5 0.09379775931370121 >10k+ 3.23919566280959E-5 4.807694318357105 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1061722 4.530700890067767 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30658 0.13082730497031955 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 28247 0.12053881151727497 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.01898954619081225 1.2801941252232978E-5 0.0 0.0 8.534627501488652E-6 2 0.019070625152076394 4.267313750744326E-5 0.0 0.0 8.534627501488652E-6 3 0.019104763662082348 4.694045125818759E-5 0.0 0.0 2.133656875372163E-5 4 0.019151704113340537 9.81482162671195E-5 0.0 4.267313750744326E-6 2.9871196255210284E-5 5 0.019160238740842026 1.3655404002381843E-4 0.0 8.534627501488652E-6 2.9871196255210284E-5 6 0.01922424844710319 1.450886675253071E-4 0.0 8.534627501488652E-6 3.413851000595461E-5 7 0.01923278307460468 1.621579225282844E-4 4.267313750744326E-6 1.2801941252232978E-5 3.413851000595461E-5 8 0.01923278307460468 1.7069255002977304E-4 4.267313750744326E-6 1.2801941252232978E-5 3.840582375669894E-5 9 0.019249852329607655 1.96296432534239E-4 4.267313750744326E-6 1.7069255002977303E-5 4.267313750744326E-5 10-11 0.019256253300233773 2.1336568753721632E-4 4.267313750744326E-6 2.3470225629093795E-5 4.267313750744326E-5 12-13 0.019290391810239726 2.368359131663101E-4 4.267313750744326E-6 2.5603882504465957E-5 4.694045125818759E-5 14-15 0.019350134202750148 2.5817248192003175E-4 4.267313750744326E-6 2.5603882504465957E-5 4.907410813355975E-5 16-17 0.019452549732768012 2.8591002129986986E-4 4.267313750744326E-6 2.9871196255210284E-5 5.334142188430408E-5 18-19 0.01952082675277992 3.072465900535915E-4 4.267313750744326E-6 3.413851000595461E-5 6.827702001190921E-5 20-21 0.019612573998420924 3.648553256886399E-4 4.267313750744326E-6 4.4806794382815425E-5 6.827702001190921E-5 22-23 0.019911285960973024 4.1179577694682747E-4 4.267313750744326E-6 4.694045125818759E-5 8.321261813951436E-5 24-25 0.021451786224991727 4.566025713296429E-4 4.267313750744326E-6 4.694045125818759E-5 1.0241553001786383E-4 26-27 0.02715718470973689 4.928747382109697E-4 4.267313750744326E-6 4.694045125818759E-5 1.0668284376860816E-4 28-29 0.04424777628146792 5.2274593446618E-4 4.267313750744326E-6 5.1207765008931914E-5 1.1095015751935247E-4 30-31 0.08316567768825617 5.334142188430408E-4 4.267313750744326E-6 5.760873563504841E-5 1.1735112814546898E-4 32-33 0.15752788710872678 5.50483473846018E-4 4.267313750744326E-6 6.827702001190921E-5 1.2375209877158545E-4 34-35 0.2772473743858589 6.080922094810666E-4 4.267313750744326E-6 6.827702001190921E-5 1.3015306939770195E-4 36-37 0.44839225967321084 6.379634057362768E-4 4.267313750744326E-6 6.827702001190921E-5 1.3442038314844627E-4 38-39 0.673676554516256 6.849038569944644E-4 4.267313750744326E-6 7.254433376265354E-5 1.4082135377456277E-4 40-41 0.9545319426783692 7.147750532496746E-4 4.267313750744326E-6 7.894530438877005E-5 1.4295501064993493E-4 42-43 1.2914662344958892 7.57448190757118E-4 4.267313750744326E-6 8.10789612641422E-5 1.4722232440067925E-4 44-45 1.68381799633745 7.787847595108395E-4 4.267313750744326E-6 8.534627501488652E-5 1.5789060877754006E-4 46-47 2.1301982175771847 8.25725210769027E-4 4.267313750744326E-6 8.961358876563085E-5 1.6002426565291225E-4 48-49 2.633286771250561 8.598637207749818E-4 4.267313750744326E-6 9.174724564100302E-5 1.6855889315440088E-4 50-51 3.184017749294122 9.004032014070529E-4 4.267313750744326E-6 9.601455939174734E-5 1.7709352065588954E-4 52-53 3.7790669142960387 9.345417114130074E-4 4.267313750744326E-6 9.81482162671195E-5 1.792271775312617E-4 54-55 4.413492716932948 9.601455939174734E-4 4.267313750744326E-6 9.81482162671195E-5 1.8349449128200605E-4 56-57 5.08875244485073 9.857494764219394E-4 4.267313750744326E-6 9.81482162671195E-5 1.8989546190812252E-4 58-59 5.800009097912916 0.0010241553001786381 4.267313750744326E-6 9.81482162671195E-5 1.9843008940961115E-4 60-61 6.543242866566305 0.0010582938101845929 4.267313750744326E-6 1.0241553001786383E-4 2.0909837378647197E-4 62-63 7.316117396532364 0.001109501575193525 4.267313750744326E-6 1.0668284376860816E-4 2.0909837378647197E-4 64-65 8.113202531063271 0.0011671103108285732 4.267313750744326E-6 1.0881650064398032E-4 2.1336568753721632E-4 66-67 8.934903664965345 0.0011948478502084112 4.267313750744326E-6 1.1095015751935249E-4 2.1549934441258847E-4 68-69 9.777484765049813 0.0012076497914606443 4.267313750744326E-6 1.152174712700968E-4 2.2190031503870498E-4 70-71 10.639742448838962 0.001231120017089738 4.267313750744326E-6 1.152174712700968E-4 2.2190031503870498E-4 72-73 11.519814033880593 0.001269525840846437 4.267313750744326E-6 1.152174712700968E-4 2.261676287894493E-4 74-75 12.4107864718985 0.0012929960664755308 4.267313750744326E-6 1.152174712700968E-4 2.325685994155658E-4 76-77 13.31495344403371 0.0013292682333568575 4.267313750744326E-6 1.2375209877158545E-4 2.368359131663101E-4 78-79 14.231062493700378 0.0013548721158613236 4.267313750744326E-6 1.258857556469576E-4 2.4110322691705443E-4 80-81 15.159092284329748 0.0013954115964933947 4.267313750744326E-6 1.280194125223298E-4 2.4537054066779874E-4 82-83 16.096926228301456 0.001425282792748605 4.267313750744326E-6 1.280194125223298E-4 2.5390516816928743E-4 84-85 17.04077281734796 0.0014551539890038153 4.267313750744326E-6 1.4082135377456277E-4 2.6243979567077606E-4 86-87 17.991794894466345 0.0014850251852590256 4.267313750744326E-6 1.4082135377456277E-4 2.709744231722647E-4 88 18.7020060556596 0.0015106290677634915 4.267313750744326E-6 1.450886675253071E-4 2.7310808004763686E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 141710 0.0 82.60228 8 GTGGTAT 142590 0.0 82.12591 6 AGTGGTA 143530 0.0 81.736145 5 TGGTATC 143465 0.0 81.44064 7 GTATCAA 143470 0.0 81.41456 9 AAGCAGT 147110 0.0 80.70051 1 CAGTGGT 150260 0.0 78.33043 4 GCAGTGG 151700 0.0 78.03946 3 AGCAGTG 156450 0.0 76.03144 2 CAACGCA 139590 0.0 43.807026 12-13 ATCAACG 141170 0.0 43.452415 10-11 ACGCAGA 142295 0.0 42.934044 14-15 AGAGTAC 142165 0.0 42.403576 18-19 TCAACGC 139565 0.0 41.80332 12-13 AGTACTT 140205 0.0 41.594845 20-21 GAGTACT 134030 0.0 41.13413 20-21 AACGCAG 141460 0.0 41.044956 14-15 CGCAGAG 143630 0.0 40.244987 16-17 TATCAAC 144935 0.0 40.240616 10-11 GTACTTT 138135 0.0 39.75495 22-23 >>END_MODULE