FastQCFastQC Report
Thu 26 May 2016
SRR1660657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23433946
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10572344.5115491859544266No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT293430.12521578738809075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA291620.12444340359920604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1415850.081.7353068
GTGGTAT1423100.081.543396
AGTGGTA1430450.081.265625
TGGTATC1428800.081.0273747
GTATCAA1430650.080.686179
AAGCAGT1459450.080.447971
GCAGTGG1505400.077.7532653
CAGTGGT1500850.077.6509254
AGCAGTG1549200.075.881862
CAACGCA1393150.043.60590412-13
ATCAACG1403950.043.29541810-11
ACGCAGA1422900.042.7333914-15
AGAGTAC1430200.042.15892418-19
TCAACGC1387700.041.5207612-13
AGTACTT1407350.041.38920220-21
GAGTACT1344200.040.9201120-21
AACGCAG1409400.040.78265414-15
CGCAGAG1437550.039.93558516-17
TATCAAC1447100.039.92506410-11
GCAGAGT1538550.039.44082616-17
Produced by FastQC (version 0.11.5)