##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23433946
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80073637619546	34.0	31.0	34.0	31.0	34.0
2	32.98166164588755	34.0	31.0	34.0	31.0	34.0
3	33.03633455500836	34.0	33.0	34.0	31.0	34.0
4	36.40940074710422	37.0	37.0	37.0	35.0	37.0
5	36.33470560186492	37.0	37.0	37.0	35.0	37.0
6	36.3278585262593	37.0	37.0	37.0	35.0	37.0
7	36.25971217139444	37.0	37.0	37.0	35.0	37.0
8	36.291107993506515	37.0	37.0	37.0	35.0	37.0
9	38.07548634788183	39.0	38.0	39.0	37.0	39.0
10-11	37.995283060735915	39.0	38.0	39.0	35.0	39.0
12-13	38.06251567704389	39.0	38.0	39.0	35.0	39.0
14-15	39.58793320595686	41.0	40.0	41.0	37.0	41.0
16-17	39.5631969536842	41.0	40.0	41.0	37.0	41.0
18-19	39.523325947751175	41.0	40.0	41.0	37.0	41.0
20-21	39.473886066819475	41.0	39.5	41.0	36.5	41.0
22-23	39.32649804262586	41.0	39.0	41.0	36.0	41.0
24-25	39.33613282628542	41.0	39.0	41.0	36.0	41.0
26-27	39.30348638253241	41.0	39.0	41.0	36.0	41.0
28-29	39.23842343922786	41.0	39.0	41.0	36.0	41.0
30-31	39.15230256142094	40.0	39.0	41.0	36.0	41.0
32-33	39.05859691748031	40.0	39.0	41.0	35.0	41.0
34-35	38.95857746279692	40.0	38.5	41.0	35.0	41.0
36-37	38.80305986452303	40.0	38.0	41.0	35.0	41.0
38-39	38.459451707365034	40.0	38.0	41.0	35.0	41.0
40-41	38.335817685164926	40.0	38.0	41.0	34.5	41.0
42-43	38.202713042865255	40.0	37.0	41.0	34.0	41.0
44-45	38.07284599017169	40.0	37.0	41.0	34.0	41.0
46-47	37.93360424659167	40.0	37.0	41.0	33.5	41.0
48-49	37.76879905330498	40.0	36.5	41.0	33.0	41.0
50-51	37.78954048114645	40.0	36.5	41.0	33.0	41.0
52-53	37.65157020503504	40.0	36.0	41.0	33.0	41.0
54-55	37.13407936077006	40.0	36.0	41.0	32.0	41.0
56-57	36.76707998729706	39.5	36.0	41.0	31.0	41.0
58-59	36.52041839219054	39.0	35.0	41.0	31.0	41.0
60-61	36.28789455689622	39.0	35.0	41.0	30.5	41.0
62-63	36.02353807591773	38.5	35.0	40.5	30.0	41.0
64-65	35.74544272227989	37.5	35.0	40.0	30.0	41.0
66-67	35.46575019845143	37.0	35.0	40.0	30.0	41.0
68-69	35.17541375660761	36.5	35.0	39.0	29.0	41.0
70-71	34.8624268614428	36.0	35.0	39.0	29.0	41.0
72-73	34.51344252905592	35.5	34.0	38.5	29.0	40.5
74-75	34.16426467399046	35.0	34.0	37.0	29.0	40.0
76-77	32.31963991467762	34.5	32.0	35.5	25.5	38.5
78-79	33.44847303992251	35.0	34.0	36.0	28.5	39.0
80-81	33.415372703342406	35.0	34.0	36.0	29.0	38.5
82-83	33.19242860336027	35.0	34.0	36.0	28.5	37.0
84-85	32.969155280122266	35.0	34.0	35.0	27.5	37.0
86-87	32.782655938526105	35.0	34.0	35.0	27.0	36.5
88-89	32.62016610433429	35.0	34.0	35.0	27.0	36.0
90-91	32.50601345586441	35.0	34.0	35.0	27.0	36.0
92-93	32.40686775500805	35.0	34.0	35.0	26.5	36.0
94-95	32.30350545742488	35.0	34.0	35.0	26.0	35.0
96-97	32.2220331991889	35.0	34.0	35.0	25.0	35.0
98-99	32.119455383229095	35.0	33.0	35.0	25.0	35.0
100	32.062699811632235	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37739743966459827
1101	2	0.321985492107288
1101	3	0.3054255933461718
1101	4	0.22460636335628692
1101	5	0.23661039871206668
1101	6	0.2520521703428571
1101	7	0.2483365061081173
1101	8	0.26585604339869917
1101	9	0.33883019960659766
1101	10-11	0.3327954842427374
1101	12-13	0.32430111562069186
1101	14-15	0.4027106626445871
1101	16-17	0.4157031961644435
1101	18-19	0.4196016339677513
1101	20-21	0.43489525093697523
1101	22-23	0.42895531708943935
1101	24-25	0.4483838168521146
1101	26-27	0.4334981634990669
1101	28-29	0.46932509907588127
1101	30-31	0.46585787952061253
1101	32-33	0.4758970279821071
1101	34-35	0.46303188005462914
1101	36-37	0.5089210722049629
1101	38-39	0.5007718506422663
1101	40-41	0.5157778974912475
1101	42-43	0.5189073038517193
1101	44-45	0.5160396165571299
1101	46-47	0.4874086459385438
1101	48-49	0.4874605743234639
1101	50-51	0.46400862715983493
1101	52-53	0.47275249303990563
1101	54-55	0.3572993197145209
1101	56-57	0.3446771071382031
1101	58-59	0.3651593424517827
1101	60-61	0.33597278032166855
1101	62-63	0.2836878987793128
1101	64-65	0.2913889835477832
1101	66-67	0.30726177212047645
1101	68-69	0.3051431903133732
1101	70-71	0.2917865295580313
1101	72-73	0.32094279407048276
1101	74-75	0.30655934191965173
1101	76-77	0.39333281466041825
1101	78-79	0.42538027419624314
1101	80-81	0.3638008333067262
1101	82-83	0.3967357704108494
1101	84-85	0.35296978339825813
1101	86-87	0.3963731905482675
1101	88-89	0.43803553502282
1101	90-91	0.432553466464519
1101	92-93	0.37253186642493574
1101	94-95	0.35100787998974425
1101	96-97	0.3718707661994145
1101	98-99	0.3685713742740404
1101	100	0.37688800940070877
1102	1	0.3195479409634814
1102	2	0.25460680316309237
1102	3	0.2735073240876389
1102	4	0.19984794754690682
1102	5	0.21207977980439097
1102	6	0.21086278706130912
1102	7	0.23056503638920844
1102	8	0.19839343067447146
1102	9	0.2694736857198876
1102	10-11	0.28865345680846133
1102	12-13	0.29284327478988104
1102	14-15	0.3794170850231211
1102	16-17	0.40138287997100974
1102	18-19	0.39274835557058907
1102	20-21	0.39227590499038456
1102	22-23	0.41994482243134
1102	24-25	0.39960905370763555
1102	26-27	0.4008289644959149
1102	28-29	0.4107045771367268
1102	30-31	0.4020425868594799
1102	32-33	0.41235009008479295
1102	34-35	0.4451010266795947
1102	36-37	0.41498062608754793
1102	38-39	0.4300853647179679
1102	40-41	0.4541239997959252
1102	42-43	0.42943422280153243
1102	44-45	0.47661285783073737
1102	46-47	0.448613805725131
1102	48-49	0.44492980134359783
1102	50-51	0.4248821317178084
1102	52-53	0.4637549260347953
1102	54-55	0.3206272107178805
1102	56-57	0.3117435005439546
1102	58-59	0.36121301743119716
1102	60-61	0.29700842360936974
1102	62-63	0.35688419051384557
1102	64-65	0.2900687602321099
1102	66-67	0.25216264483771766
1102	68-69	0.2817143094385699
1102	70-71	0.3059941658908656
1102	72-73	0.30009998686031025
1102	74-75	0.3214974701194748
1102	76-77	0.3321497431465019
1102	78-79	0.350984558526406
1102	80-81	0.3384042470866291
1102	82-83	0.3227136851051853
1102	84-85	0.36301568239439774
1102	86-87	0.4020721794733504
1102	88-89	0.38475638550979596
1102	90-91	0.3584763988970323
1102	92-93	0.3321816282249799
1102	94-95	0.40342353910745743
1102	96-97	0.43071524383190507
1102	98-99	0.42975075028060417
1102	100	0.427318969137481
1103	1	0.2728619247698205
1103	2	0.2485839460012258
1103	3	0.24083010033231034
1103	4	0.1499104870359531
1103	5	0.17465775243764625
1103	6	0.18840385993556907
1103	7	0.20510015267444004
1103	8	0.16538609095447754
1103	9	0.23587700203908213
1103	10-11	0.2684909427401294
1103	12-13	0.27193516761509784
1103	14-15	0.32748571275956095
1103	16-17	0.2967417550939899
1103	18-19	0.30718789714278927
1103	20-21	0.3410182434498026
1103	22-23	0.3504401230781795
1103	24-25	0.33417683861101466
1103	26-27	0.31334436864764825
1103	28-29	0.3447668740860621
1103	30-31	0.3446842084454147
1103	32-33	0.34716072087829986
1103	34-35	0.36097851262015723
1103	36-37	0.33354048815358084
1103	38-39	0.3653993903168242
1103	40-41	0.3601594383393447
1103	42-43	0.3478573175846833
1103	44-45	0.3772607538662456
1103	46-47	0.37391501550979456
1103	48-49	0.346001857362225
1103	50-51	0.38522661039451833
1103	52-53	0.3612546221421127
1103	54-55	0.26415835546145416
1103	56-57	0.28755092472039223
1103	58-59	0.3476408138083258
1103	60-61	0.29855274057415215
1103	62-63	0.24428141901375255
1103	64-65	0.27767214341934476
1103	66-67	0.24606961368102276
1103	68-69	0.23701644037122804
1103	70-71	0.24736570143920744
1103	72-73	0.27376719157863505
1103	74-75	0.2486670799591124
1103	76-77	0.1949729333631467
1103	78-79	0.34281240075717534
1103	80-81	0.3260382363073475
1103	82-83	0.339317259799941
1103	84-85	0.3155062960247932
1103	86-87	0.32129974648365334
1103	88-89	0.3759828031281316
1103	90-91	0.3232081226188157
1103	92-93	0.27000386101239116
1103	94-95	0.2696509342059059
1103	96-97	0.2666637124136102
1103	98-99	0.29610171430955745
1103	100	0.30176413232447885
1104	1	0.22891541196155885
1104	2	0.20370705636832298
1104	3	0.19012388646603284
1104	4	0.14883678448402549
1104	5	0.13851256231154707
1104	6	0.14024271056640458
1104	7	0.1700109902967455
1104	8	0.1446475675523402
1104	9	0.18381044663266977
1104	10-11	0.19571037902619537
1104	12-13	0.18366689337855036
1104	14-15	0.22120563067218058
1104	16-17	0.2225436654054107
1104	18-19	0.2327799712093821
1104	20-21	0.2587428819935198
1104	22-23	0.24578286046912723
1104	24-25	0.2665488678287744
1104	26-27	0.24833963515873592
1104	28-29	0.2542455448479899
1104	30-31	0.24982752341422554
1104	32-33	0.2663324992320426
1104	34-35	0.296370572112572
1104	36-37	0.29884098052014707
1104	38-39	0.27224487563109534
1104	40-41	0.2612198512541255
1104	42-43	0.23670265483351471
1104	44-45	0.277027238520823
1104	46-47	0.239765231415781
1104	48-49	0.2643418565698141
1104	50-51	0.2642752820088603
1104	52-53	0.27997849793666774
1104	54-55	0.14196478932580447
1104	56-57	0.1704396961047081
1104	58-59	0.1769679181808712
1104	60-61	0.1235565528490099
1104	62-63	0.10830057613715383
1104	64-65	0.1372736443420166
1104	66-67	0.16887590658825502
1104	68-69	0.13366293449964672
1104	70-71	0.14718048076783674
1104	72-73	0.14574122700553005
1104	74-75	0.16318650670118018
1104	76-77	0.16749356302406682
1104	78-79	0.2621328516594943
1104	80-81	0.22001153296285736
1104	82-83	0.24900328974120356
1104	84-85	0.22143446427190128
1104	86-87	0.22374160523740017
1104	88-89	0.2147543735659454
1104	90-91	0.2193925062789006
1104	92-93	0.20311210725617457
1104	94-95	0.23336373604664828
1104	96-97	0.2414974737058202
1104	98-99	0.2175302813392861
1104	100	0.25544338402720257
1105	1	0.1768057501680289
1105	2	0.12305429040377192
1105	3	0.1013144864304465
1105	4	0.1150070834712551
1105	5	0.09658894163531073
1105	6	0.11642859823136575
1105	7	0.1219478677770951
1105	8	0.13340565269157878
1105	9	0.15093041995768175
1105	10-11	0.1787097244603757
1105	12-13	0.1649855255137922
1105	14-15	0.20707085904466993
1105	16-17	0.2102574199175109
1105	18-19	0.20777537242635447
1105	20-21	0.21136969659807647
1105	22-23	0.20574909001548036
1105	24-25	0.23519476522879756
1105	26-27	0.22688333084608558
1105	28-29	0.20781644002897082
1105	30-31	0.21517942707789928
1105	32-33	0.22777736573877405
1105	34-35	0.2418045823457291
1105	36-37	0.22749084825983346
1105	38-39	0.262159286295514
1105	40-41	0.23770032430854116
1105	42-43	0.25292370455412083
1105	44-45	0.28006146427108547
1105	46-47	0.24030180852892613
1105	48-49	0.24837818483580065
1105	50-51	0.24055284444734326
1105	52-53	0.22434995678825942
1105	54-55	0.18010808042965465
1105	56-57	0.2042253547947297
1105	58-59	0.17300380824608652
1105	60-61	0.10891298486080814
1105	62-63	0.05294734993871231
1105	64-65	0.12920046194177104
1105	66-67	0.13036070215679274
1105	68-69	0.058830131889806125
1105	70-71	0.12754720926676555
1105	72-73	0.14531719185755776
1105	74-75	0.14522610834331573
1105	76-77	0.1016586196038034
1105	78-79	0.24379238525467173
1105	80-81	0.25317926565820414
1105	82-83	0.2515771311521817
1105	84-85	0.27392433429833574
1105	86-87	0.2934699706815209
1105	88-89	0.28352461932353634
1105	90-91	0.22662018617953805
1105	92-93	0.2278888286473304
1105	94-95	0.22181580209351637
1105	96-97	0.24235567842025318
1105	98-99	0.25922502964532157
1105	100	0.27315474375296844
1106	1	0.24732911257944323
1106	2	0.21718566535230366
1106	3	0.20426899405855892
1106	4	0.13774014379548305
1106	5	0.15606862306796643
1106	6	0.17423434706241636
1106	7	0.14877083067441532
1106	8	0.18731402399887287
1106	9	0.2254592335649619
1106	10-11	0.20706950118906775
1106	12-13	0.23042230566593958
1106	14-15	0.2646168821295092
1106	16-17	0.25460249967837
1106	18-19	0.2780822003211654
1106	20-21	0.2515956948503586
1106	22-23	0.27160477872966027
1106	24-25	0.2548628901491554
1106	26-27	0.27390007925392723
1106	28-29	0.29083668713672495
1106	30-31	0.2659886149556314
1106	32-33	0.29836773048096177
1106	34-35	0.31040567931501784
1106	36-37	0.31104797439686394
1106	38-39	0.325011126803048
1106	40-41	0.33237327932379657
1106	42-43	0.29664476521141125
1106	44-45	0.3148666453449138
1106	46-47	0.3166185698329542
1106	48-49	0.29803104417072035
1106	50-51	0.25991999542363686
1106	52-53	0.30257270078500653
1106	54-55	0.23872007988627786
1106	56-57	0.22987969045138357
1106	58-59	0.21750181153600323
1106	60-61	0.16687688404799417
1106	62-63	0.13699330749104632
1106	64-65	0.16546783773615203
1106	66-67	0.17332070619868034
1106	68-69	0.17708158830323129
1106	70-71	0.1432058869899251
1106	72-73	0.15137930032949498
1106	74-75	0.17255934053383726
1106	76-77	0.13441741541932117
1106	78-79	0.2618659072814893
1106	80-81	0.2522302081743888
1106	82-83	0.30905880236406347
1106	84-85	0.2945590135480032
1106	86-87	0.3431333768787894
1106	88-89	0.34761956517532866
1106	90-91	0.33743097580373416
1106	92-93	0.3064919790641838
1106	94-95	0.3183126592575931
1106	96-97	0.2969943333088523
1106	98-99	0.32377431750148844
1106	100	0.32621519321516246
1107	1	0.143511194560773
1107	2	0.13169244707795258
1107	3	0.11288682437756847
1107	4	0.10642336768309946
1107	5	0.10276113907020346
1107	6	0.09050698094167586
1107	7	0.1234075830421375
1107	8	0.12936881537250144
1107	9	0.1630479271125509
1107	10-11	0.15934473633700463
1107	12-13	0.13471532840129186
1107	14-15	0.17389180023330653
1107	16-17	0.16019020519232186
1107	18-19	0.20186876439768753
1107	20-21	0.14869713571705034
1107	22-23	0.1763707045679226
1107	24-25	0.17410486781488999
1107	26-27	0.16636779998662377
1107	28-29	0.20721378854492656
1107	30-31	0.16886270277688453
1107	32-33	0.1702684012409179
1107	34-35	0.19319274890442983
1107	36-37	0.1850561545126297
1107	38-39	0.1429933571659845
1107	40-41	0.17787351627804782
1107	42-43	0.13477755358559307
1107	44-45	0.14211456998676653
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2312	24-25	-0.236142169289991
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2312	62-63	-0.26439662622363613
2312	64-65	-0.17243324966634788
2312	66-67	-0.18511894098087822
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2312	78-79	-0.2409062822263408
2312	80-81	-0.24144316199738114
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2312	100	-0.2430511711919081
2313	1	-0.20375649299061394
2313	2	-0.19441439802142213
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2313	70-71	-0.21280960085899636
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2313	74-75	-0.18795340931474414
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2313	80-81	-0.2657016410556281
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2313	84-85	-0.24379607422019944
2313	86-87	-0.21358244416972383
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2313	90-91	-0.21897747771001974
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2313	96-97	-0.2266168528794239
2313	98-99	-0.205165468080601
2313	100	-0.2760950189758624
2314	1	-0.6764828173201707
2314	2	-0.44549067200037484
2314	3	-0.3590166026547763
2314	4	-0.24183169881893463
2314	5	-0.22114599929703616
2314	6	-0.23448125348909343
2314	7	-0.21840839777960497
2314	8	-0.2321953786595614
2314	9	-0.2833496454540665
2314	10-11	-0.31057403041276643
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2314	14-15	-0.37413319950226054
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2314	18-19	-0.4475989176667525
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2314	84-85	-0.45688836524850984
2314	86-87	-0.5102431090040298
2314	88-89	-0.5594384244764115
2314	90-91	-0.42074703494861154
2314	92-93	-0.3960675426004272
2314	94-95	-0.39229111064749134
2314	96-97	-0.4051873607816532
2314	98-99	-0.3746253351649784
2314	100	-0.37242764735953315
2315	1	-0.9627640723135436
2315	2	-0.5636490000490184
2315	3	-0.4326460177650304
2315	4	-0.2683100946810342
2315	5	-0.2760115704798949
2315	6	-0.24992466908809519
2315	7	-0.25114628141738393
2315	8	-0.27181700813031995
2315	9	-0.3620207969780864
2315	10-11	-0.34991201963795504
2315	12-13	-0.34824220739606204
2315	14-15	-0.4161696451111112
2315	16-17	-0.452889364959546
2315	18-19	-0.43104393970470767
2315	20-21	-0.4456378729214592
2315	22-23	-0.48543822616079524
2315	24-25	-0.4544530930730488
2315	26-27	-0.4692452609834774
2315	28-29	-0.4792378216544435
2315	30-31	-0.4958800783248307
2315	32-33	-0.5018678179176348
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2315	90-91	-0.3832262989722466
2315	92-93	-0.38957066539187224
2315	94-95	-0.36787184404047224
2315	96-97	-0.38928876704144955
2315	98-99	-0.40062897019327437
2315	100	-0.42274272416043956
2316	1	-1.5834096193516842
2316	2	-0.8389631603596044
2316	3	-0.6026785932366892
2316	4	-0.36224249267478825
2316	5	-0.32406869543479644
2316	6	-0.3095515237997333
2316	7	-0.33316961988469984
2316	8	-0.31022827141516274
2316	9	-0.4215399101134807
2316	10-11	-0.4066415023382177
2316	12-13	-0.3777701893048615
2316	14-15	-0.5135002495152392
2316	16-17	-0.5417496890620512
2316	18-19	-0.5132271429542996
2316	20-21	-0.5356559425521894
2316	22-23	-0.55674326466454
2316	24-25	-0.5611563554370065
2316	26-27	-0.5797020833611057
2316	28-29	-0.5955215131397082
2316	30-31	-0.6153686334389192
2316	32-33	-0.5789010082544976
2316	34-35	-0.5654520327450214
2316	36-37	-0.5699925285051606
2316	38-39	-0.6256508732454833
2316	40-41	-0.6135235866029802
2316	42-43	-0.6067714826448594
2316	44-45	-0.5655498960709835
2316	46-47	-0.5716255358895097
2316	48-49	-0.587947309493579
2316	50-51	-0.5813699886425994
2316	52-53	-0.5417171722180569
2316	54-55	-0.528532049569165
2316	56-57	-0.5411331442459186
2316	58-59	-0.5435329302119385
2316	60-61	-0.5093029245756355
2316	62-63	-0.4816176115328332
2316	64-65	-0.451132806743999
2316	66-67	-0.48977250210664636
2316	68-69	-0.4646798787578703
2316	70-71	-0.49087879561704995
2316	72-73	-0.5480072145422241
2316	74-75	-0.5368003093778881
2316	76-77	-0.578035787198047
2316	78-79	-0.7531961979675046
2316	80-81	-0.7220199425795997
2316	82-83	-0.700098867560591
2316	84-85	-0.7022633211704559
2316	86-87	-0.6847546613059876
2316	88-89	-0.7804327551965002
2316	90-91	-0.6923142433256615
2316	92-93	-0.6227561304696252
2316	94-95	-0.6218542176632518
2316	96-97	-0.6652724645995107
2316	98-99	-1.4527590366175716
2316	100	-1.0311471147701035
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	9.0
7	261.0
8	380.0
9	141.0
10	73.0
11	71.0
12	96.0
13	359.0
14	1344.0
15	4390.0
16	11092.0
17	18266.0
18	22567.0
19	28155.0
20	35499.0
21	49267.0
22	75369.0
23	127793.0
24	228746.0
25	326630.0
26	346106.0
27	292378.0
28	250429.0
29	255570.0
30	292258.0
31	351966.0
32	433386.0
33	559424.0
34	789124.0
35	1317827.0
36	2664039.0
37	5967091.0
38	7269905.0
39	1711939.0
40	1996.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.241721208711986	31.193716804224316	27.911911271855622	23.652650715208075
2	22.420673778888187	33.0500205710475	24.756151733959516	19.773153916104803
3	26.519558421786925	24.451592574293716	26.26796187035679	22.76088713356257
4	20.817970648221173	27.027458371714264	23.551944687420548	28.60262629264401
5	22.967941464062434	30.703544336920462	25.438690521860895	20.88982367715621
6	28.82866163470719	26.780436380624927	24.40894930798253	19.981952676685353
7	22.436208566837184	25.024859236254958	30.486602640460127	22.052329556447727
8	27.76162409864732	26.331340014182842	24.264061204203507	21.642974682966326
9	26.641078715466872	24.913431139595527	26.2361917194825	22.209298425455106
10-11	21.888110521377836	28.7920224788433	27.57412686706712	21.74574013271175
12-13	21.698046500576556	26.189434762715592	27.627239987665757	24.485278749042095
14-15	21.695076450206038	31.301019469789683	25.13966491174811	21.86423916825617
16-17	24.454769589381147	25.846856948462715	25.25745557320991	24.44091788894623
18-19	21.763692793983687	28.50993243670264	25.32848491096885	24.397889858344822
20-21	24.35655640700076	28.679091326191298	25.26666875911932	21.697683507688623
22-23	24.286095634210792	26.092613355215725	27.84518113046185	21.776109880111637
24-25	21.822112821642026	28.31492132663744	25.237615009031312	24.62535084268923
26-27	21.87712813106459	25.813357756511817	30.511972415271167	21.797541697152433
28-29	21.956204995715016	25.883769630893678	30.4028373678751	21.757188005516205
30-31	21.828834827559977	25.816101031350858	30.489378207467887	21.86568593362128
32-33	21.93988985510475	26.011578245251194	30.24737460430675	21.801157295337305
34-35	21.90920212137505	26.143589811215133	30.15640684510319	21.79080122230663
36-37	21.864333482335784	26.11252294038824	30.250827714728906	21.772315862547075
38-39	21.91549333163699	26.109696817980225	30.278480798723884	21.696329051658893
40-41	21.93957742068977	26.19538544062454	30.215978085546336	21.649059053139357
42-43	21.960177351029795	26.212479301075447	30.148221703854393	21.679121644040368
44-45	22.012845341081057	26.321701401877558	30.060821846272702	21.604631410768686
46-47	22.055670058167667	26.456411658647216	29.917785822730664	21.570132460454452
48-49	22.056473649045557	26.510340846276105	29.801664021465662	21.63152148321268
50-51	22.136095702565687	26.608349853248352	29.65735640798386	21.598198036202103
52-53	22.238880354948197	26.754203919916076	29.327683752908495	21.679231972227235
54-55	22.437219773135844	27.041333413734197	28.66528135378993	21.85616545934003
56-57	22.774334011857945	27.535683949112773	27.711839910938224	21.978142128091065
58-59	22.877601563057652	27.92022798107801	27.160775177698532	22.0413952781658
60-61	22.861025686478012	28.10893409231604	26.935611882584748	22.094428338621203
62-63	22.794387828227812	28.29970008216234	26.785404495472186	22.120507594137663
64-65	22.78078867554631	28.382739674951157	26.675228716284234	22.161242933218297
66-67	22.671461101094412	28.272780845427686	26.831561248731113	22.22419680474679
68-69	22.56550013715059	28.218769349860075	26.98579130125739	22.229939211731942
70-71	22.503475736132895	28.168407407830415	27.043032095040846	22.28508476099584
72-73	22.428585222542242	28.167842719559278	27.01710387740592	22.386468180492557
74-75	22.4688699928405	28.28075860849728	26.80855975000557	22.44181164865665
76-77	22.451400082557633	28.415237414655447	26.594108362186965	22.539254140599954
78-79	22.474750152842667	28.58028406767554	26.268340186171553	22.676625593310245
80-81	22.50852036804482	28.76971247637339	25.966847834355377	22.754919321226417
82-83	22.49564848656008	28.881366706152367	25.702415924757577	22.92056888252998
84-85	22.4606603174213	28.939229992690755	25.538235358380046	23.0618743315079
86-87	22.48177628597532	28.990718498371553	25.38287151712183	23.14463369853129
88-89	22.452197089915142	29.018421393799947	25.278905251758964	23.25047626452595
90-91	22.444390902110516	28.95704128135283	25.229421575082604	23.369146241454047
92-93	22.447041177058754	28.964419545422288	25.17465383216785	23.41388544535111
94-95	22.44079579141369	28.937809651144924	25.134953585747404	23.48644097169398
96-97	22.38605094988818	28.877687145064407	25.159914248111466	23.576347656935944
98-99	22.389400870922323	28.837711643153654	25.165085969909466	23.60780151601456
100	22.29294535731703	28.852138709596474	25.147208207608635	23.70770772547786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32185.0
1	21875.0
2	9625.0
3	7258.0
4	6829.5
5	6756.0
6	6855.5
7	7012.5
8	6897.0
9	7044.5
10	7588.0
11	11553.0
12	104565.5
13	122898.0
14	49963.5
15	50923.0
16	56334.0
17	61613.5
18	67307.0
19	73194.0
20	80560.5
21	90751.0
22	103435.5
23	117341.5
24	132454.5
25	149917.0
26	170081.0
27	194462.5
28	223359.0
29	251408.5
30	276669.0
31	302679.0
32	331351.5
33	359878.0
34	386326.0
35	416940.0
36	452387.0
37	483300.0
38	516365.0
39	552520.0
40	583667.5
41	616222.0
42	649487.0
43	678971.0
44	708571.0
45	743008.0
46	777944.5
47	810452.0
48	844790.5
49	876251.0
50	905374.0
51	927756.5
52	930941.0
53	915174.5
54	885080.0
55	844137.0
56	789883.0
57	718363.0
58	633366.0
59	542118.5
60	443394.5
61	348596.0
62	268146.5
63	199120.5
64	143725.0
65	102503.0
66	71808.0
67	49783.0
68	34810.5
69	24683.5
70	18003.0
71	13781.0
72	10905.5
73	8140.5
74	6174.5
75	4843.0
76	3567.0
77	2328.0
78	1469.5
79	922.0
80	514.5
81	312.5
82	188.0
83	107.0
84	62.0
85	36.0
86	26.0
87	15.5
88	9.5
89	8.5
90	8.0
91	6.5
92	4.5
93	4.5
94	3.0
95	3.0
96	2.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10708397126117813
2	0.07484868318805549
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.944446488013585E-4
20-21	0.0022211368072624217
22-23	8.001213282645613E-4
24-25	8.043886420153055E-4
26-27	0.006659143108036521
28-29	0.006505519813009726
30-31	0.0077387734869748355
32-33	0.007730238859473347
34-35	0.007218161209384027
36-37	0.010941392456908451
38-39	0.01331615255919767
40-41	0.0167705430404252
42-43	0.0234467554034647
44-45	0.011636964598279778
46-47	0.018560681158862447
48-49	0.01612831232093818
50-51	0.018761244905147433
52-53	0.021121069409309044
54-55	0.11196364453515426
56-57	0.10487563639516795
58-59	0.10212961999656396
60-61	0.14445710509019694
62-63	0.09544914031977371
64-65	0.07439848158735195
66-67	0.08412582328217365
68-69	0.07714876529970667
70-71	0.05397511797628961
72-73	0.0322694265831286
74-75	0.021097599183679948
76-77	0.012981168429764241
78-79	0.00926220449599056
80-81	0.006057451869181571
82-83	6.187604938579273E-4
84-85	0.002603061387954039
86-87	0.0017047918434223582
88-89	0.002304349425401936
90-91	0.003307168156826853
92-93	0.00497142051961714
94-95	0.002214735836636305
96-97	0.0012503229289680875
98-99	0.0016983908727962419
100	0.0020056374628498333
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.3433946E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.874903204794684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.49819072459044	40.20211142384847
2	11.562669691203972	11.996247420404387
3	4.159052376240332	6.472513183234158
4	2.0016687988082453	4.153455007449415
5	1.180442110606024	3.0617660113275527
6	0.7236631786172132	2.252397440618519
7	0.5143151847803377	1.8676035299065277
8	0.3835262633315842	1.5916310229458022
9	0.2811244520027826	1.3124973362530767
>10	1.5362918519949968	14.628905608682153
>50	0.12929472750542267	4.511112676294507
>100	0.028714660152058865	2.454841780321825
>500	5.038039791295942E-4	0.17093989491346978
>1k	4.8476789292393584E-4	0.4448260858770535
>5k	2.4603554802314317E-5	0.07612745961667138
>10k+	3.2804739736153374E-5	4.803024118306264
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1057234	4.5115491859544266	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29343	0.12521578738809075	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29162	0.12444340359920604	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.019138902172088305	2.133656875372163E-5	0.0	0.0	1.2801941252232978E-5
2	0.019207179192100212	2.9871196255210284E-5	0.0	8.534627501488652E-6	2.133656875372163E-5
3	0.019241317702106166	5.1207765008931914E-5	0.0	8.534627501488652E-6	2.5603882504465957E-5
4	0.019279723525862866	8.10789612641422E-5	0.0	8.534627501488652E-6	2.5603882504465957E-5
5	0.01928399083961361	1.1948478502084113E-4	0.0	8.534627501488652E-6	2.5603882504465957E-5
6	0.01936506980087775	1.1948478502084113E-4	0.0	8.534627501488652E-6	3.840582375669894E-5
7	0.01936506980087775	1.493559812760514E-4	0.0	8.534627501488652E-6	4.267313750744326E-5
8	0.01937360442837924	1.5789060877754006E-4	0.0	1.2801941252232978E-5	4.694045125818759E-5
9	0.019386406369631473	1.7069255002977304E-4	0.0	1.2801941252232978E-5	4.694045125818759E-5
10-11	0.019394940997132962	2.0483106003572766E-4	0.0	1.9202911878349465E-5	4.694045125818759E-5
12-13	0.019439747791515777	2.3896957004168227E-4	0.0	2.133656875372163E-5	4.907410813355975E-5
14-15	0.019488821899649338	2.5177151129391527E-4	0.0	2.133656875372163E-5	5.974239251042057E-5
16-17	0.01958056914529034	2.645734525461483E-4	0.0	2.9871196255210284E-5	7.254433376265354E-5
18-19	0.019636044224050016	2.944446488013585E-4	0.0	4.05394806320711E-5	8.534627501488652E-5
20-21	0.01973845975406788	3.5418704131177903E-4	0.0	4.267313750744326E-5	8.961358876563085E-5
22-23	0.02004570634412147	3.883255513177337E-4	0.0	4.907410813355975E-5	9.174724564100302E-5
24-25	0.021586206608140174	4.160630906975718E-4	0.0	5.1207765008931914E-5	9.81482162671195E-5
26-27	0.027298006063511456	4.416669732020378E-4	0.0	5.760873563504841E-5	1.0668284376860816E-4
28-29	0.044322454272105946	4.630035419557594E-4	0.0	5.974239251042057E-5	1.1521747127009682E-4
30-31	0.08335770680703966	5.142113069646914E-4	0.0	5.974239251042057E-5	1.2375209877158545E-4
32-33	0.1575662929324835	5.419488463445294E-4	0.0	5.974239251042057E-5	1.258857556469576E-4
34-35	0.27727297826836333	5.547507875967625E-4	0.0	6.40097062611649E-5	1.322867262730741E-4
36-37	0.4482172998094303	5.696863857243676E-4	0.0	6.827702001190921E-5	1.3655404002381843E-4
38-39	0.6728700322173653	5.910229544780892E-4	0.0	7.254433376265354E-5	1.3655404002381843E-4
40-41	0.9526052505199082	6.14493180107183E-4	0.0	7.254433376265354E-5	1.386876968991906E-4
42-43	1.2878134139252517	6.614336313653706E-4	0.0	7.254433376265354E-5	1.4082135377456277E-4
44-45	1.6783451664521203	6.998394551220694E-4	0.0	7.894530438877005E-5	1.450886675253071E-4
46-47	2.1221884696670377	7.531808770063736E-4	0.0	8.534627501488652E-5	1.493559812760514E-4
48-49	2.6219485186148335	7.915867007630725E-4	0.0	8.961358876563085E-5	1.5148963815142356E-4
50-51	3.1691034877352706	8.278588676443993E-4	0.0	8.961358876563085E-5	1.6855889315440088E-4
52-53	3.760871088462865	8.491954363981209E-4	0.0	8.961358876563085E-5	1.7709352065588954E-4
54-55	4.391938515177939	8.747993189025868E-4	0.0	8.961358876563085E-5	1.792271775312617E-4
56-57	5.063058948757499	9.174724564100301E-4	0.0	9.174724564100302E-5	1.8776180503275036E-4
58-59	5.771228200321022	9.516109664159847E-4	0.0	9.601455939174734E-5	1.9416277565886684E-4
60-61	6.511184672013838	9.964177607988001E-4	0.0	9.81482162671195E-5	1.96296432534239E-4
62-63	7.280077798250453	0.001034823584555499	0.0	9.81482162671195E-5	1.96296432534239E-4
64-65	8.07389843776204	0.0010689620945614538	0.0	9.81482162671195E-5	2.069647169110998E-4
66-67	8.892973040050531	0.0010753630651875702	0.0	1.0028187314249166E-4	2.1123203066184416E-4
68-69	9.733461022740258	0.001098833290816664	0.0	1.04549186893236E-4	2.2190031503870498E-4
70-71	10.59377707877282	0.001109501575193525	0.0	1.1095015751935249E-4	2.2403397191407713E-4
72-73	11.471209330259615	0.0011393727714487352	0.0	1.152174712700968E-4	2.2830128566482145E-4
74-75	12.359851814969616	0.0011649766539532011	0.0	1.1948478502084113E-4	2.304349425401936E-4
76-77	13.262915686500259	0.0012140507620867608	0.0	1.1948478502084113E-4	2.304349425401936E-4
78-79	14.17752904269729	0.0012439219583419711	0.0	1.258857556469576E-4	2.3470225629093795E-4
80-81	15.105232383824731	0.0012908624096001587	0.0	1.280194125223298E-4	2.4110322691705443E-4
82-83	16.042445433645703	0.0013442038314844629	4.267313750744326E-6	1.3442038314844627E-4	2.4323688379242659E-4
84-85	16.9852358625389	0.0013740750277396732	4.267313750744326E-6	1.3655404002381843E-4	2.5177151129391527E-4
86-87	17.93666973543423	0.0013996789102441389	4.267313750744326E-6	1.3655404002381843E-4	2.5817248192003175E-4
88	18.64857928750028	0.001412480851496372	4.267313750744326E-6	1.3655404002381843E-4	2.645734525461482E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	141585	0.0	81.735306	8
GTGGTAT	142310	0.0	81.54339	6
AGTGGTA	143045	0.0	81.26562	5
TGGTATC	142880	0.0	81.027374	7
GTATCAA	143065	0.0	80.68617	9
AAGCAGT	145945	0.0	80.44797	1
GCAGTGG	150540	0.0	77.753265	3
CAGTGGT	150085	0.0	77.650925	4
AGCAGTG	154920	0.0	75.88186	2
CAACGCA	139315	0.0	43.605904	12-13
ATCAACG	140395	0.0	43.295418	10-11
ACGCAGA	142290	0.0	42.73339	14-15
AGAGTAC	143020	0.0	42.158924	18-19
TCAACGC	138770	0.0	41.52076	12-13
AGTACTT	140735	0.0	41.389202	20-21
GAGTACT	134420	0.0	40.92011	20-21
AACGCAG	140940	0.0	40.782654	14-15
CGCAGAG	143755	0.0	39.935585	16-17
TATCAAC	144710	0.0	39.925064	10-11
GCAGAGT	153855	0.0	39.440826	16-17
>>END_MODULE