FastQCFastQC Report
Thu 26 May 2016
SRR1660656_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660656_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27038561
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9162023.388501333336489No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA319610.11820525507995785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1250300.081.0028
GTGGTAT1260350.080.4134456
AGTGGTA1272750.079.73435
GTATCAA1272600.079.386619
TGGTATC1273350.079.362387
AAGCAGT1309850.078.489361
GCAGTGG1351750.075.7855453
CAGTGGT1381250.073.710794
AGCAGTG1422050.072.363752
CAACGCA1231550.043.21617512-13
ATCAACG1245700.042.80661810-11
ACGCAGA1270550.041.8470814-15
AGAGTAC1260350.041.52755718-19
TCAACGC1227050.041.17481612-13
AACGCAG1250950.040.22275514-15
GAGTACT1193150.040.01864620-21
AGTACTT1283150.039.50234620-21
TATCAAC1289700.039.1930110-11
CGCAGAG1290450.038.88358716-17
GTACTTT1247100.038.233422-23
Produced by FastQC (version 0.11.5)