##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660656_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27038561
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.421360700371594	34.0	31.0	34.0	31.0	34.0
2	32.5789437537005	34.0	31.0	34.0	31.0	34.0
3	32.600571938721146	34.0	31.0	34.0	31.0	34.0
4	35.95439986617631	37.0	37.0	37.0	35.0	37.0
5	35.9500775947359	37.0	37.0	37.0	35.0	37.0
6	35.964313633406746	37.0	37.0	37.0	35.0	37.0
7	35.926437061498945	37.0	37.0	37.0	35.0	37.0
8	35.94139932224944	37.0	37.0	37.0	35.0	37.0
9	37.67755136821076	39.0	38.0	39.0	35.0	39.0
10-11	37.61873568271625	39.0	38.0	39.0	35.0	39.0
12-13	37.66486180607023	39.0	38.0	39.0	35.0	39.0
14-15	39.156258888925336	41.0	39.0	41.0	36.0	41.0
16-17	39.116007301572	41.0	39.0	41.0	36.0	41.0
18-19	39.08482964385568	41.0	39.0	41.0	36.0	41.0
20-21	39.02427207572178	41.0	39.0	41.0	36.0	41.0
22-23	38.898734958565285	40.5	39.0	41.0	35.5	41.0
24-25	38.884946373440506	40.0	39.0	41.0	35.0	41.0
26-27	38.843445607183014	40.0	39.0	41.0	35.0	41.0
28-29	38.76236840044853	40.0	39.0	41.0	35.0	41.0
30-31	38.67058528003764	40.0	38.0	41.0	35.0	41.0
32-33	38.56487640004215	40.0	38.0	41.0	34.0	41.0
34-35	38.46076196510606	40.0	38.0	41.0	34.0	41.0
36-37	38.314153478803846	40.0	38.0	41.0	34.0	41.0
38-39	38.000102982551475	40.0	37.5	41.0	33.5	41.0
40-41	37.86277376225755	40.0	37.0	41.0	33.0	41.0
42-43	37.716052769968044	40.0	37.0	41.0	33.0	41.0
44-45	37.5747209882952	40.0	37.0	41.0	33.0	41.0
46-47	37.40799789604188	40.0	36.0	41.0	32.0	41.0
48-49	37.4278173494514	40.0	36.0	41.0	32.0	41.0
50-51	36.46292034550211	38.5	35.5	40.0	31.5	40.5
52-53	36.67010200727768	39.0	35.5	39.5	31.5	40.5
54-55	37.13946343150436	40.0	36.0	41.0	31.5	41.0
56-57	36.90639745953936	40.0	36.0	41.0	31.0	41.0
58-59	36.69352714443642	39.5	35.5	41.0	30.0	41.0
60-61	36.4718084849264	39.0	35.0	41.0	30.0	41.0
62-63	36.20516533775596	39.0	35.0	41.0	30.0	41.0
64-65	35.916738394472986	38.0	35.0	41.0	29.0	41.0
66-67	35.6114853708376	37.0	35.0	40.0	29.0	41.0
68-69	35.30621905137629	37.0	35.0	40.0	29.0	41.0
70-71	34.9679023598926	36.0	35.0	39.0	29.0	41.0
72-73	34.6110449258006	36.0	34.5	39.0	28.5	41.0
74-75	34.249909897202	35.0	34.0	38.0	28.0	40.0
76-77	33.89058426593043	35.0	34.0	37.0	27.5	39.5
78-79	33.54678690186212	35.0	34.0	37.0	27.0	39.0
80-81	33.22836799635898	35.0	34.0	36.0	26.5	39.0
82-83	32.9589562477086	35.0	34.0	36.0	26.0	37.0
84-85	32.72101779011095	35.0	33.5	35.5	26.0	37.0
86-87	32.518100353047636	35.0	33.0	35.0	25.5	36.5
88-89	32.33002303635907	35.0	33.0	35.0	25.0	36.0
90-91	32.19737603639484	35.0	33.0	35.0	25.0	36.0
92-93	32.0699600470602	35.0	33.0	35.0	25.0	36.0
94-95	31.964554899944567	35.0	33.0	35.0	24.0	35.5
96-97	31.862840592737165	35.0	33.0	35.0	24.0	35.0
98-99	31.621082460712316	35.0	33.0	35.0	23.0	35.0
100	31.55661786143131	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5004151982766558
1101	2	0.49532535030751745
1101	3	0.48283368634724155
1101	4	0.43278065668098975
1101	5	0.41370081553861837
1101	6	0.3998611622231891
1101	7	0.40405410661879415
1101	8	0.4165490847685973
1101	9	0.5357139723354933
1101	10-11	0.4974472846585485
1101	12-13	0.48954803598959273
1101	14-15	0.6339877324410637
1101	16-17	0.6499938027602568
1101	18-19	0.6325751985091941
1101	20-21	0.6355456578707717
1101	22-23	0.6505474498557362
1101	24-25	0.662169210300739
1101	26-27	0.6419924628880835
1101	28-29	0.6330353514216966
1101	30-31	0.637534934223325
1101	32-33	0.6312244186254148
1101	34-35	0.6172965267053314
1101	36-37	0.6471257165717503
1101	38-39	0.6807481250661169
1101	40-41	0.6599522259361663
1101	42-43	0.6930696659268136
1101	44-45	0.6498223022488148
1101	46-47	0.6729830134723684
1101	48-49	0.6538880169890007
1101	50-51	0.6598079497960967
1101	52-53	0.6953670809538792
1101	54-55	0.7219952798405131
1101	56-57	0.7157813403731339
1101	58-59	0.6690951925382294
1101	60-61	0.6778152380660529
1101	62-63	0.6335460359639526
1101	64-65	0.6290217565841587
1101	66-67	0.6161214958962447
1101	68-69	0.5601497484204856
1101	70-71	0.5826580945940094
1101	72-73	0.5308584218714429
1101	74-75	0.510771187609464
1101	76-77	0.49906741848843694
1101	78-79	0.5250527665664961
1101	80-81	0.5182683369404586
1101	82-83	0.47207744663787565
1101	84-85	0.47842268338492744
1101	86-87	0.5051132729308776
1101	88-89	0.4601063224894162
1101	90-91	0.46456333407379446
1101	92-93	0.45057365910139424
1101	94-95	0.4487793746879376
1101	96-97	0.45901350879555025
1101	98-99	0.2731862697706724
1101	100	0.33305809611376347
1102	1	0.4599964056652013
1102	2	0.41426717304835137
1102	3	0.45210544039088063
1102	4	0.400599860601325
1102	5	0.38661960010758634
1102	6	0.35670404553521706
1102	7	0.36703963508909254
1102	8	0.39243688762801554
1102	9	0.4491842002680997
1102	10-11	0.49240223839854025
1102	12-13	0.46407218585897425
1102	14-15	0.5325388622356328
1102	16-17	0.5759219107819646
1102	18-19	0.5746238655945248
1102	20-21	0.5472470662302982
1102	22-23	0.5630027349169211
1102	24-25	0.5809773867353485
1102	26-27	0.5765906780530159
1102	28-29	0.582715374531368
1102	30-31	0.5853353747585786
1102	32-33	0.5856312660141043
1102	34-35	0.5717041738309518
1102	36-37	0.5929471082384481
1102	38-39	0.6089276109939519
1102	40-41	0.6201099443917713
1102	42-43	0.6347337001542712
1102	44-45	0.5894934912996277
1102	46-47	0.6070450168670334
1102	48-49	0.6089322716335204
1102	50-51	0.582308711305835
1102	52-53	0.63237288893
1102	54-55	0.719926651411555
1102	56-57	0.6970878482451752
1102	58-59	0.6649777700586483
1102	60-61	0.6392970941601348
1102	62-63	0.6522061523286737
1102	64-65	0.6313100137555168
1102	66-67	0.604385233444539
1102	68-69	0.6127431337699107
1102	70-71	0.6012866636490557
1102	72-73	0.6045092472782372
1102	74-75	0.5759799752804184
1102	76-77	0.5866253792816991
1102	78-79	0.5567887882173039
1102	80-81	0.5600156324681578
1102	82-83	0.5127105484439411
1102	84-85	0.5371882285845899
1102	86-87	0.5424126651249424
1102	88-89	0.5305394926338565
1102	90-91	0.4875026487583938
1102	92-93	0.4985013261646287
1102	94-95	0.5099426349888745
1102	96-97	0.5259455096027637
1102	98-99	0.3526886902348494
1102	100	0.40145980134511206
1103	1	0.39862421565857176
1103	2	0.39678794107800996
1103	3	0.38057207943920446
1103	4	0.3403044905640016
1103	5	0.37158722295133373
1103	6	0.32618830152194533
1103	7	0.3371903517141277
1103	8	0.3538877075598208
1103	9	0.437336161503616
1103	10-11	0.4365050894370981
1103	12-13	0.41005287321343786
1103	14-15	0.5118528583812392
1103	16-17	0.5332897240611416
1103	18-19	0.5054049508485718
1103	20-21	0.5027256680885159
1103	22-23	0.5067515838842098
1103	24-25	0.5115331046487697
1103	26-27	0.4891542292286104
1103	28-29	0.52312861944948
1103	30-31	0.4982791065829417
1103	32-33	0.4949640960924384
1103	34-35	0.5085596721223737
1103	36-37	0.4799204387154532
1103	38-39	0.48329331675058995
1103	40-41	0.5287211884809437
1103	42-43	0.5381071453999411
1103	44-45	0.5177060486307923
1103	46-47	0.5234007495995598
1103	48-49	0.4982619846328191
1103	50-51	0.4500669371109325
1103	52-53	0.5501148276248315
1103	54-55	0.5716734410056432
1103	56-57	0.5414116671261269
1103	58-59	0.5070347059330089
1103	60-61	0.5083142279928339
1103	62-63	0.45450710429931007
1103	64-65	0.48796280121728586
1103	66-67	0.49050352045333767
1103	68-69	0.4736086608442349
1103	70-71	0.4886863456495689
1103	72-73	0.44869069327961597
1103	74-75	0.4368506726700758
1103	76-77	0.416778698586711
1103	78-79	0.38917051548548187
1103	80-81	0.37869404771460324
1103	82-83	0.40560724224487643
1103	84-85	0.41093989255617913
1103	86-87	0.3891173813088926
1103	88-89	0.4102462616345406
1103	90-91	0.4127240305962019
1103	92-93	0.3895055693852427
1103	94-95	0.37111487035113555
1103	96-97	0.37901519244739745
1103	98-99	0.24614631800351816
1103	100	0.28028747689054256
1104	1	0.3642832408005958
1104	2	0.3437904086258641
1104	3	0.3426358456879086
1104	4	0.31753717959070826
1104	5	0.3040372099736217
1104	6	0.29527292320619836
1104	7	0.30074283388344014
1104	8	0.31330026924185717
1104	9	0.3791214807943959
1104	10-11	0.3906786913292919
1104	12-13	0.35793202965113835
1104	14-15	0.47268075286076083
1104	16-17	0.46433983249995947
1104	18-19	0.4549153825143364
1104	20-21	0.45685718745789927
1104	22-23	0.47223837034732696
1104	24-25	0.46057080736926537
1104	26-27	0.46862634945497916
1104	28-29	0.47434675302341134
1104	30-31	0.46501642154978384
1104	32-33	0.45633042432033477
1104	34-35	0.48566878185313556
1104	36-37	0.4719817575840892
1104	38-39	0.48379551115515795
1104	40-41	0.47845314305720876
1104	42-43	0.4844521051934194
1104	44-45	0.45776528458060284
1104	46-47	0.4558042107310385
1104	48-49	0.47528822967859696
1104	50-51	0.3865900289754123
1104	52-53	0.48834366616544145
1104	54-55	0.548040844160802
1104	56-57	0.5042123513207315
1104	58-59	0.5394853523300895
1104	60-61	0.509187145273863
1104	62-63	0.5368476651284624
1104	64-65	0.5184321051664753
1104	66-67	0.4901719097232089
1104	68-69	0.49519305456194473
1104	70-71	0.49305354966379156
1104	72-73	0.4565902585118238
1104	74-75	0.43265698142549525
1104	76-77	0.41755568753073646
1104	78-79	0.4246495423419532
1104	80-81	0.41914629543546056
1104	82-83	0.447976135249327
1104	84-85	0.4542592441165709
1104	86-87	0.44979720242639587
1104	88-89	0.41846569869899497
1104	90-91	0.4528207469555028
1104	92-93	0.4649129956447169
1104	94-95	0.4041798499020004
1104	96-97	0.4059219041389923
1104	98-99	0.28813201179966796
1104	100	0.36752383177090664
1105	1	0.3104138544413715
1105	2	0.3080211367440384
1105	3	0.32435465222670246
1105	4	0.29626705677719656
1105	5	0.2976074426953659
1105	6	0.27307341714957545
1105	7	0.2688606563964697
1105	8	0.2817876516569555
1105	9	0.34874142800500607
1105	10-11	0.3284390987218515
1105	12-13	0.356519682228587
1105	14-15	0.43574426370550157
1105	16-17	0.4177010624659516
1105	18-19	0.43385834491076736
1105	20-21	0.3982349831940937
1105	22-23	0.42048476896984255
1105	24-25	0.40418109931901114
1105	26-27	0.40349932794099885
1105	28-29	0.4143807939728461
1105	30-31	0.4463978370074244
1105	32-33	0.45515057541002335
1105	34-35	0.42850038238306354
1105	36-37	0.4489654707714976
1105	38-39	0.45338009012664315
1105	40-41	0.469935637108847
1105	42-43	0.41876633656108453
1105	44-45	0.44655356309881
1105	46-47	0.4430217418057154
1105	48-49	0.3849381641525582
1105	50-51	0.30214831675056786
1105	52-53	0.3834547827426462
1105	54-55	0.4259833157067803
1105	56-57	0.4114224536887292
1105	58-59	0.4377581677030733
1105	60-61	0.38343489787570206
1105	62-63	0.369650066523846
1105	64-65	0.3835463179959149
1105	66-67	0.38706624040542437
1105	68-69	0.3550782695536796
1105	70-71	0.337359734946304
1105	72-73	0.33535677080037374
1105	74-75	0.34417789764224693
1105	76-77	0.36286013551281826
1105	78-79	0.355937409701788
1105	80-81	0.35339388960631624
1105	82-83	0.33242737707765
1105	84-85	0.3013149598537126
1105	86-87	0.35434785787802525
1105	88-89	0.3605623156987079
1105	90-91	0.3172485654293382
1105	92-93	0.310310163609536
1105	94-95	0.32118169727824153
1105	96-97	0.30583230546014306
1105	98-99	0.18747992202073505
1105	100	0.19880749454979707
1106	1	0.33596847310230515
1106	2	0.3030799589773352
1106	3	0.329322124759571
1106	4	0.2892441500950653
1106	5	0.2724930483273127
1106	6	0.26331168027142837
1106	7	0.2784013273900783
1106	8	0.2955670583734644
1106	9	0.3561230912288309
1106	10-11	0.36748058179153986
1106	12-13	0.342958873902667
1106	14-15	0.4053838261756937
1106	16-17	0.4476003545902074
1106	18-19	0.4332915530099868
1106	20-21	0.45265866806107624
1106	22-23	0.45960953647752945
1106	24-25	0.4329634086174252
1106	26-27	0.4005663621523823
1106	28-29	0.43349200207539695
1106	30-31	0.43799597874519236
1106	32-33	0.44943836915555835
1106	34-35	0.4661249666022371
1106	36-37	0.43924677885344465
1106	38-39	0.4396428078916159
1106	40-41	0.49523761674949185
1106	42-43	0.4773427903807388
1106	44-45	0.4912512272760807
1106	46-47	0.5236348097263246
1106	48-49	0.49126434384847073
1106	50-51	0.4776076669531406
1106	52-53	0.4764974132392652
1106	54-55	0.534532279576311
1106	56-57	0.5494355264483204
1106	58-59	0.5040373250363999
1106	60-61	0.5459237155436725
1106	62-63	0.5225673322192392
1106	64-65	0.4851552227535407
1106	66-67	0.500707905946328
1106	68-69	0.476596338496833
1106	70-71	0.475728266165504
1106	72-73	0.4715827594908646
1106	74-75	0.4744306524774444
1106	76-77	0.4654401281341052
1106	78-79	0.4605518389527532
1106	80-81	0.4843449056786113
1106	82-83	0.4720740324108945
1106	84-85	0.5210794233621172
1106	86-87	0.48632379363289857
1106	88-89	0.512094157698705
1106	90-91	0.5021739433603329
1106	92-93	0.49151401502295755
1106	94-95	0.5239563448793874
1106	96-97	0.5176902738258349
1106	98-99	0.42556727380924997
1106	100	0.4646099433774502
1107	1	0.2943691827684489
1107	2	0.26286210002882626
1107	3	0.25860664455570515
1107	4	0.25371295291756724
1107	5	0.2814253206171031
1107	6	0.23477464574644102
1107	7	0.21635087339589631
1107	8	0.2567099402445976
1107	9	0.32912454042301675
1107	10-11	0.3645332094186031
1107	12-13	0.2966682915608132
1107	14-15	0.3694469601972301
1107	16-17	0.37777648047984513
1107	18-19	0.38927117917165077
1107	20-21	0.3767478962122084
1107	22-23	0.3589704923140289
1107	24-25	0.35886899180137277
1107	26-27	0.36591247332390964
1107	28-29	0.3583838635723353
1107	30-31	0.39927054610296153
1107	32-33	0.38017845017314045
1107	34-35	0.3703465882942254
1107	36-37	0.38520095477208827
1107	38-39	0.3659709140127845
1107	40-41	0.39514909928999487
1107	42-43	0.3863099942538639
1107	44-45	0.4342715918539426
1107	46-47	0.43572082998151274
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2309	54-55	-0.002662001129060343
2309	56-57	4.588305616195498E-4
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2309	100	0.1324923418222852
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2310	100	0.1318649959455449
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2311	2	-0.12545063252058952
2311	3	-0.1502797310350772
2311	4	-0.09426488435482128
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2311	7	-0.09090810710069519
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2311	10-11	-0.13344720221720507
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2311	14-15	-0.15458492637019816
2311	16-17	-0.1399817903284557
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2311	20-21	-0.13812691398678112
2311	22-23	-0.1529109707918508
2311	24-25	-0.1675952407375405
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2311	28-29	-0.1748234252196923
2311	30-31	-0.17151713581392158
2311	32-33	-0.1837576963963201
2311	34-35	-0.21742466225911272
2311	36-37	-0.17810488662070156
2311	38-39	-0.21011252582338358
2311	40-41	-0.13814648845757205
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2311	78-79	-0.09901435166584349
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2311	86-87	-0.0706331006638834
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2312	7	-0.12696873319459456
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2312	10-11	-0.1927742723430299
2312	12-13	-0.22237030859425744
2312	14-15	-0.2131359925141254
2312	16-17	-0.2269805805696663
2312	18-19	-0.23824078882048383
2312	20-21	-0.2816655704606106
2312	22-23	-0.23402351202042126
2312	24-25	-0.20556225302323128
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2312	28-29	-0.2570158559872411
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2312	40-41	-0.32688542585867
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2312	44-45	-0.27502808312566174
2312	46-47	-0.32337091094860426
2312	48-49	-0.24448291864065652
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2312	52-53	-0.3280061226603337
2312	54-55	-0.32581384796183244
2312	56-57	-0.3365895016163307
2312	58-59	-0.3003799114977497
2312	60-61	-0.36103202696056513
2312	62-63	-0.2968614356858552
2312	64-65	-0.2909316591718252
2312	66-67	-0.27266050812860954
2312	68-69	-0.23958021511803906
2312	70-71	-0.27148203995282927
2312	72-73	-0.27003180644523184
2312	74-75	-0.1866888992713811
2312	76-77	-0.2097352564068018
2312	78-79	-0.21534375946960438
2312	80-81	-0.19625005941748697
2312	82-83	-0.21322232643454697
2312	84-85	-0.23432055604766333
2312	86-87	-0.2067861004358349
2312	88-89	-0.23548036593183497
2312	90-91	-0.27474966672521006
2312	92-93	-0.1732353723630382
2312	94-95	-0.1788616210311602
2312	96-97	-0.2080760136597668
2312	98-99	-0.07304556051508015
2312	100	-0.11831620339590998
2313	1	-0.2558385614624328
2313	2	-0.2690186052675543
2313	3	-0.2503345395811465
2313	4	-0.19377173175036688
2313	5	-0.23504604451928657
2313	6	-0.2133198973863415
2313	7	-0.18888885865562344
2313	8	-0.16789896145826333
2313	9	-0.24229112170044687
2313	10-11	-0.22292865491589708
2313	12-13	-0.22626895861525753
2313	14-15	-0.25465668609428604
2313	16-17	-0.2844619001895623
2313	18-19	-0.29190382845374074
2313	20-21	-0.2889910912111304
2313	22-23	-0.2933983363119097
2313	24-25	-0.2914061296310422
2313	26-27	-0.26507034859177736
2313	28-29	-0.28583706297281
2313	30-31	-0.3223405986696477
2313	32-33	-0.2885621512711012
2313	34-35	-0.30574657088963875
2313	36-37	-0.3001996590321383
2313	38-39	-0.2872700796929095
2313	40-41	-0.3598869478981044
2313	42-43	-0.273780819002873
2313	44-45	-0.25221500771612426
2313	46-47	-0.2822835361650817
2313	48-49	-0.3111234628753081
2313	50-51	-0.24632478766191213
2313	52-53	-0.33175619283953495
2313	54-55	-0.3353328777478666
2313	56-57	-0.3628403992536491
2313	58-59	-0.37535002359816616
2313	60-61	-0.3746156256133091
2313	62-63	-0.28354366355279126
2313	64-65	-0.31734703768456995
2313	66-67	-0.27857519247683626
2313	68-69	-0.23416501703930237
2313	70-71	-0.3064830909215459
2313	72-73	-0.2667661238476171
2313	74-75	-0.28395183330343343
2313	76-77	-0.2550224838838133
2313	78-79	-0.24272537064735644
2313	80-81	-0.3096066317398183
2313	82-83	-0.22287377452688162
2313	84-85	-0.2441536364580017
2313	86-87	-0.20929485840422046
2313	88-89	-0.20218489359173475
2313	90-91	-0.21157280734315265
2313	92-93	-0.2543109707952098
2313	94-95	-0.19524065474097796
2313	96-97	-0.20706031690126991
2313	98-99	-0.09319341011806159
2313	100	-0.09610068799672788
2314	1	-0.32559910622978094
2314	2	-0.3457208137868051
2314	3	-0.32552581577094486
2314	4	-0.3246715476337769
2314	5	-0.28569518025284424
2314	6	-0.30418959156559566
2314	7	-0.3152194192581277
2314	8	-0.29903694852056617
2314	9	-0.46993949741196417
2314	10-11	-0.429214821954254
2314	12-13	-0.3896944341597717
2314	14-15	-0.4621444889313935
2314	16-17	-0.4504155837917807
2314	18-19	-0.40795151881973624
2314	20-21	-0.4344744148018762
2314	22-23	-0.413680491667364
2314	24-25	-0.41101768765226865
2314	26-27	-0.42831503569732376
2314	28-29	-0.44364159355323096
2314	30-31	-0.4283916156587182
2314	32-33	-0.46746364541655083
2314	34-35	-0.5147211766318378
2314	36-37	-0.47337796923121545
2314	38-39	-0.41842716009675485
2314	40-41	-0.4061239922738338
2314	42-43	-0.42852834546145147
2314	44-45	-0.38795038061250153
2314	46-47	-0.40381805480208044
2314	48-49	-0.4164979631825645
2314	50-51	-0.4264319863449657
2314	52-53	-0.45903782201708054
2314	54-55	-0.5018719310003874
2314	56-57	-0.5032860685212057
2314	58-59	-0.4406207024663047
2314	60-61	-0.41990767418443653
2314	62-63	-0.44175523054327925
2314	64-65	-0.4075806906390369
2314	66-67	-0.37403662488299005
2314	68-69	-0.37743680206699537
2314	70-71	-0.4117477604166737
2314	72-73	-0.3851421591269073
2314	74-75	-0.35619143708059653
2314	76-77	-0.30407379448952554
2314	78-79	-0.32501319874011614
2314	80-81	-0.3123255695159912
2314	82-83	-0.3132403727001076
2314	84-85	-0.3136618323031328
2314	86-87	-0.3082742538339005
2314	88-89	-0.30742823297911315
2314	90-91	-0.33581417239016886
2314	92-93	-0.3539995827732412
2314	94-95	-0.26912737874894077
2314	96-97	-0.27930206383071976
2314	98-99	-0.1816206600366428
2314	100	-0.21815780900942983
2315	1	-0.41728071722013027
2315	2	-0.42379819584044753
2315	3	-0.4583031892999472
2315	4	-0.34565627997608317
2315	5	-0.3583254162075491
2315	6	-0.36809569724263724
2315	7	-0.3715371717908056
2315	8	-0.34506541671206037
2315	9	-0.43797079230139246
2315	10-11	-0.39327200771304405
2315	12-13	-0.4668263760109994
2315	14-15	-0.6656208850674332
2315	16-17	-0.6332359452985585
2315	18-19	-0.6178989317569901
2315	20-21	-0.6483013923923977
2315	22-23	-0.599214035565204
2315	24-25	-0.6193846325331975
2315	26-27	-0.6219313278547318
2315	28-29	-0.608575819451282
2315	30-31	-0.6136602686230859
2315	32-33	-0.5526602375877872
2315	34-35	-0.5291639129591061
2315	36-37	-0.538560324783333
2315	38-39	-0.5417336747735249
2315	40-41	-0.6243798954386364
2315	42-43	-0.5723085119701707
2315	44-45	-0.5767822888515681
2315	46-47	-0.557563013572782
2315	48-49	-0.545804806520465
2315	50-51	-0.59667562840486
2315	52-53	-0.63183063131941
2315	54-55	-0.674381573147393
2315	56-57	-0.7139805757403224
2315	58-59	-0.6380283415357368
2315	60-61	-0.5646803748519602
2315	62-63	-0.5755493480537339
2315	64-65	-0.5570855339383982
2315	66-67	-0.5153292321137641
2315	68-69	-0.5430817709421945
2315	70-71	-0.5015317208459749
2315	72-73	-0.5135574035183055
2315	74-75	-0.4887092864306197
2315	76-77	-0.4652958536753715
2315	78-79	-0.4694638621386247
2315	80-81	-0.42728546898012354
2315	82-83	-0.43104278693872544
2315	84-85	-0.39277722878853893
2315	86-87	-0.3994369993199456
2315	88-89	-0.3752145890105929
2315	90-91	-0.4132947521444734
2315	92-93	-0.35638082179904984
2315	94-95	-0.35026461084869354
2315	96-97	-0.3922608084278032
2315	98-99	-0.31299881918536343
2315	100	-0.3422636958252916
2316	1	-0.6512135165418229
2316	2	-0.6486929980798948
2316	3	-0.6812985508969156
2316	4	-0.5599882741010163
2316	5	-0.5298522655368174
2316	6	-0.49892260886375794
2316	7	-0.5011740107707183
2316	8	-0.5009019661893248
2316	9	-0.6476676198004796
2316	10-11	-0.6645880652529215
2316	12-13	-0.553267417840317
2316	14-15	-0.7261745707293912
2316	16-17	-0.7575876516679969
2316	18-19	-0.8789515102632137
2316	20-21	-0.8201662531485638
2316	22-23	-0.7325452703330839
2316	24-25	-0.7919251832650644
2316	26-27	-0.7512765162480619
2316	28-29	-0.7874141386677778
2316	30-31	-0.7869538039431561
2316	32-33	-0.7581386363540616
2316	34-35	-0.7784459395015944
2316	36-37	-0.7257691790884024
2316	38-39	-0.7378709853763965
2316	40-41	-0.804927003747963
2316	42-43	-0.7650775836106831
2316	44-45	-0.7522365677488025
2316	46-47	-0.7295687056544438
2316	48-49	-0.7032743094079024
2316	50-51	-0.6470392035953765
2316	52-53	-0.7513604853815821
2316	54-55	-0.8100992762887813
2316	56-57	-0.7855593798546465
2316	58-59	-0.7631647530683168
2316	60-61	-0.7158870216369166
2316	62-63	-0.670822526549884
2316	64-65	-0.704920305585425
2316	66-67	-0.654528625125046
2316	68-69	-0.6568926598568083
2316	70-71	-0.755448450533315
2316	72-73	-0.661671657385142
2316	74-75	-0.598609894573741
2316	76-77	-0.6056303291405314
2316	78-79	-0.5598153487894066
2316	80-81	-1.045980222683724
2316	82-83	-0.8840962754858879
2316	84-85	-0.6433626928729694
2316	86-87	-0.5607356260248295
2316	88-89	-0.6461816963352298
2316	90-91	-0.6563735651015676
2316	92-93	-0.6398481801360703
2316	94-95	-0.5885445353090013
2316	96-97	-0.632870831780366
2316	98-99	-0.4905539968674759
2316	100	-0.5827991560770087
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	789.0
3	253.0
4	263.0
5	202.0
6	692.0
7	4955.0
8	11213.0
9	15175.0
10	17161.0
11	16796.0
12	17376.0
13	19864.0
14	23920.0
15	32095.0
16	43042.0
17	55629.0
18	62391.0
19	68819.0
20	78850.0
21	96074.0
22	124110.0
23	180312.0
24	263097.0
25	337664.0
26	349734.0
27	308504.0
28	281848.0
29	292179.0
30	336243.0
31	410203.0
32	515375.0
33	675058.0
34	959957.0
35	1553369.0
36	2992746.0
37	6394047.0
38	8151264.0
39	2342844.0
40	4448.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	16.825901535391395	30.84033161245484	28.34074631134641	23.993020540807354
2	22.29347203067998	31.90601296069276	25.840437613021226	19.960077395606035
3	25.049885421575325	25.015269046875964	26.889750545703585	23.045094985845125
4	20.662955979002582	27.417288175328196	24.431085610244995	27.488670235424223
5	22.89434756385208	29.948056654094923	26.142117897533527	21.015477884519466
6	27.356402538827663	27.104046243630386	25.292368785312597	20.247182432229355
7	22.392227950133133	25.64134471219098	29.918873096431675	22.047554241244217
8	26.31246502707385	26.673358429169436	25.212934544446636	21.80124199931008
9	25.424881480993673	25.37750717551468	26.925986573148812	22.271624770342836
10-11	21.894308127444713	28.5607450692197	27.737878645624903	21.807068157710688
12-13	21.755999628252617	26.574803757607757	27.80594231719762	23.863254296942007
14-15	21.774948012884412	30.45587853467676	25.899871037147005	21.869302415291827
16-17	23.887134900107224	26.293212490655726	25.986675562645573	23.832977046591473
18-19	21.80671553736234	28.29407465456118	26.065639585903426	23.83357022217306
20-21	23.81467189094276	28.412664837297	25.99554931807039	21.777113953689852
22-23	23.789247284700878	26.432918891326278	27.946352965146946	21.8314808588259
24-25	21.91528250490281	28.131538180951527	25.91196394198696	24.041215372158703
26-27	21.973149763012437	26.220678807959573	29.94294073571464	21.863230693313348
28-29	22.014191315726794	26.300538617186593	29.880379259237777	21.80489080784884
30-31	21.93056020445602	26.20933668051701	29.931303625752737	21.92879948927423
32-33	22.029978156482102	26.375058874521663	29.703302371720124	21.891660597276115
34-35	22.030724810065994	26.495360142116205	29.609753933320555	21.864161114497247
36-37	21.96675703456921	26.448403098668365	29.69748840059539	21.887351466167036
38-39	22.027514362667468	26.453495474459306	29.713184986533193	21.805805176340034
40-41	22.049360169618417	26.49837175635178	29.68443626878623	21.767831805243574
42-43	22.06176134755986	26.53074138317778	29.601386568997327	21.806110700265027
44-45	22.124147748769758	26.59286304974342	29.559351832846602	21.723637368640215
46-47	22.16980084205879	26.66593184455216	29.474567722147565	21.689699591241478
48-49	22.2376193738059	26.66864194940467	29.390671947684144	21.70306672910528
50-51	22.315700761256462	26.729610636678498	29.308633691223683	21.646054910841357
52-53	22.454972239696044	26.83858688260926	29.076075833965564	21.63036504372913
54-55	22.70825558970341	27.022630529069158	28.549416001412403	21.71969787981503
56-57	23.069631939585165	27.363946347087122	27.842426292209204	21.72399542111851
58-59	23.238938296334023	27.65595482823268	27.445420681624643	21.659686193808643
60-61	23.368144241310578	27.79195475661536	27.257233004381593	21.58266799769246
62-63	23.48643925166206	27.89580993711329	27.14422489494141	21.47352591628324
64-65	23.646689897300835	27.942809562061395	27.072859443835846	21.337641096801928
66-67	23.74991151709083	27.82644561282269	27.180738172843043	21.242904697243436
68-69	23.905242799903668	27.74708650103785	27.26772006290339	21.079950636155097
70-71	24.038976708780442	27.666968349229094	27.343300727876624	20.950754214113843
72-73	24.206540103496526	27.612770318288955	27.30842591219519	20.872263666019332
74-75	24.44718433110098	27.66048469230932	27.16120204562938	20.731128930960313
76-77	24.689838658179088	27.72054661189539	26.941432872574385	20.648181857351137
78-79	24.904200469075334	27.77167017697757	26.725636066763457	20.598493287183636
80-81	25.14538593537325	27.85295076169759	26.495939523091288	20.50572377983787
82-83	25.314563904783448	27.921730918371658	26.304550055299526	20.45915512154537
84-85	25.46514722645591	27.88200794476341	26.206088827044937	20.44675600173574
86-87	25.649209803612745	27.87690974501856	26.096465402294726	20.377415049073967
88-89	25.784535817175314	27.87456128866877	26.00738802988355	20.33351486427237
90-91	25.914860783034026	27.814554209956206	25.966391344301275	20.304193662708492
92-93	26.03557979771432	27.849559237064575	25.92148222075617	20.193378744464933
94-95	26.102062078431675	27.880019462438828	25.882783146510608	20.135135312618893
96-97	26.181050683453467	27.906811937124086	25.81365199168375	20.098485387738695
98-99	26.278639347063432	27.989076764189974	25.72021192824192	20.012071960504677
100	26.30128521914642	28.071679390008537	25.627139843467745	19.999895547377303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35999.0
1	24313.0
2	10354.0
3	7435.0
4	6784.5
5	6715.0
6	6777.5
7	7218.0
8	7278.5
9	7270.5
10	7873.5
11	11723.5
12	94833.5
13	110544.0
14	44733.0
15	45311.5
16	50490.5
17	55701.0
18	60311.0
19	65763.0
20	73502.0
21	84620.0
22	100585.0
23	119370.5
24	138183.0
25	159626.5
26	182683.5
27	209878.0
28	250115.5
29	293712.5
30	328434.0
31	365506.5
32	407537.0
33	442662.5
34	476805.0
35	526690.0
36	582865.0
37	620587.0
38	655672.0
39	695148.5
40	726836.0
41	764083.5
42	812919.0
43	841707.0
44	856672.0
45	888361.0
46	925331.0
47	959687.0
48	983602.5
49	998007.0
50	1009267.0
51	1014452.5
52	1010044.0
53	992448.5
54	957035.0
55	906871.0
56	845758.0
57	774651.5
58	691576.5
59	593587.5
60	491065.0
61	395886.5
62	311038.5
63	234555.0
64	173644.0
65	128170.0
66	92597.0
67	68179.0
68	51073.5
69	38567.5
70	29825.5
71	24718.0
72	20942.5
73	15940.0
74	12244.5
75	10548.0
76	7792.0
77	4339.0
78	2529.0
79	1778.5
80	1297.5
81	1010.0
82	747.5
83	547.5
84	449.5
85	414.0
86	363.5
87	314.0
88	277.5
89	234.5
90	191.5
91	154.5
92	131.0
93	112.0
94	101.0
95	79.5
96	56.5
97	47.0
98	35.5
99	27.0
100	19.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1369969355987547
2	0.05093096485423171
3	0.01890633159064937
4	0.07606913696331694
5	0.0799820670929936
6	0.08135418153355128
7	0.048083180166281776
8	0.04548318973040023
9	0.03380727250980554
10-11	0.023030071755667767
12-13	0.015065520683589633
14-15	0.012043910177024584
16-17	0.017101501814390196
18-19	0.016393254064075376
20-21	0.02329635811609945
22-23	0.01962197618430951
24-25	0.016901787044066436
26-27	0.01525783861056807
28-29	0.017397375548203177
30-31	0.015133941484533883
32-33	0.012239926525675683
34-35	0.009828555595099903
36-37	0.0075595738989216186
38-39	0.016637349894471083
40-41	0.02524727554842878
42-43	0.025687387727475586
44-45	0.024233908010119324
46-47	0.02616818254492168
48-49	0.022723102756836804
50-51	0.022976444641414163
52-53	0.019474039317403023
54-55	0.024422527515425098
56-57	0.030989075195236908
58-59	0.02354600157900415
60-61	0.023216842050137212
62-63	0.030646971190515647
64-65	0.0312442662906506
66-67	0.024056383769831536
68-69	0.02452423411142331
70-71	0.03549930042504851
72-73	0.039917065113043555
74-75	0.0409951550306246
76-77	0.04207879258071463
78-79	0.03644239795157738
80-81	0.03183601375827656
82-83	0.034746671614661745
84-85	0.038158465607692656
86-87	0.03801977479496783
88-89	0.03739289232145157
90-91	0.04035162965958136
92-93	0.0335261924626832
94-95	0.03350954956515622
96-97	0.033540986149373855
98-99	0.3150777883482779
100	0.15043330153553658
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.7038561E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.32622667157827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46091451838761	37.433937132033485
2	10.904446099221941	10.53941467838014
3	4.112851353252028	5.962757602912948
4	2.1610270663851936	4.177371354141868
5	1.275547151349391	3.082119038319831
6	0.812225289651022	2.355107007363816
7	0.5634761047632717	1.9061471773965495
8	0.4112853837243355	1.5900696544455428
9	0.3182645597887934	1.3842472732094977
>10	1.7586630104981313	15.526840253521188
>50	0.13640768533330344	4.540460710323914
>100	0.08401516300170875	7.327991828091518
>500	4.084997320944427E-4	0.12838122450212222
>1k	4.2991833752292817E-4	0.3616616710850924
>5k	7.639314712072631E-6	0.0278988762543388
>10k+	3.055725884828844E-5	3.655594518018145
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	916202	3.388501333336489	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31961	0.11820525507995785	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.004700693945953707	7.39684334532448E-6	0.0	0.0	7.39684334532448E-6
2	0.004719186054317018	1.1095265017986719E-5	0.0	0.0	7.39684334532448E-6
3	0.004719186054317018	1.479368669064896E-5	0.0	3.69842167266224E-6	7.39684334532448E-6
4	0.00472288447598968	1.84921083633112E-5	0.0	3.69842167266224E-6	1.1095265017986719E-5
5	0.00472288447598968	2.2190530035973438E-5	0.0	3.69842167266224E-6	1.1095265017986719E-5
6	0.00472288447598968	3.69842167266224E-5	0.0	3.69842167266224E-6	1.479368669064896E-5
7	0.004726582897662342	4.8079481744609114E-5	0.0	3.69842167266224E-6	1.84921083633112E-5
8	0.004726582897662342	5.177790341727135E-5	0.0	3.69842167266224E-6	2.5888951708635677E-5
9	0.004726582897662342	6.657159010792032E-5	0.0	7.39684334532448E-6	2.958737338129792E-5
10-11	0.004732130530171336	7.027001178058255E-5	0.0	9.2460541816556E-6	3.328579505396016E-5
12-13	0.004741376584352992	8.691290930756263E-5	0.0	1.1095265017986719E-5	3.5135005890291274E-5
14-15	0.004759868692716303	9.800817432554934E-5	0.0	1.294447585431784E-5	4.6230270908278E-5
16-17	0.004780210011915944	1.0540501767087384E-4	0.0	1.479368669064896E-5	5.917474676259583E-5
18-19	0.004802400541951918	1.2019870436152279E-4	0.0	1.479368669064896E-5	6.657159010792032E-5
20-21	0.004852329234532858	1.3869081272483398E-4	0.0	2.0341319199642318E-5	7.39684334532448E-5
22-23	0.005105671119110222	1.5348449941548294E-4	0.0	2.2190530035973438E-5	7.766685512590704E-5
24-25	0.006327999481925092	1.775242402877875E-4	0.0	2.2190530035973438E-5	9.800817432554934E-5
26-27	0.010790145229992085	1.9786555948742983E-4	0.0	2.5888951708635677E-5	1.0910343934353607E-4
28-29	0.023882557951216413	2.0896082450541653E-4	0.0	2.958737338129792E-5	1.2019870436152279E-4
30-31	0.05317405759870135	2.2930214370505886E-4	0.0	3.143658421762904E-5	1.2759554770684726E-4
32-33	0.10842847738827521	2.4964346290470114E-4	0.0	3.69842167266224E-5	1.3314318021584063E-4
34-35	0.19707039882780744	2.644371495953502E-4	0.0	4.0682638399284636E-5	1.4608765607015847E-4
36-37	0.3249322328950864	2.8847689046765466E-4	0.0	4.4381060071946875E-5	1.6088134276080743E-4
38-39	0.4938576427939342	3.1251663133995925E-4	0.0	4.4381060071946875E-5	1.6642897526980078E-4
40-41	0.7086046480062308	3.199134746852837E-4	0.0	4.4381060071946875E-5	1.775242402877875E-4
42-43	0.9682190557404293	3.3470716137593264E-4	0.0	4.4381060071946875E-5	1.8122266196044973E-4
44-45	1.2729449618269255	3.5135005890291277E-4	0.0	4.4381060071946875E-5	1.9046871614210534E-4
46-47	1.6275847668076713	3.679929564298929E-4	0.0	4.8079481744609114E-5	2.052624028327543E-4
48-49	2.0319387559123427	3.7908822144787954E-4	0.0	5.177790341727135E-5	2.145084570144099E-4
50-51	2.4833144781632424	3.994295406475219E-4	0.0	5.547632508993359E-5	2.2190530035973437E-4
52-53	2.9779968689901803	4.197708598471642E-4	0.0	5.547632508993359E-5	2.348497762140522E-4
54-55	3.5152388472152793	4.586042874101177E-4	0.0	5.547632508993359E-5	2.4039740872304558E-4
56-57	4.094674269092945	4.8819166079141563E-4	0.0	6.1023957598926954E-5	2.4409583039570782E-4
58-59	4.7164307301708845	5.011361366457335E-4	0.0	6.287316843525807E-5	2.5704030625002566E-4
60-61	5.376608244795276	5.122314016637202E-4	0.0	6.657159010792032E-5	2.699847821043435E-4
62-63	6.072667846487836	5.196282450090447E-4	0.0	7.027001178058255E-5	2.7183399294067464E-4
64-65	6.799028616944518	5.381203533723559E-4	0.0	7.211922261691366E-5	2.810800471223302E-4
66-67	7.554447886483308	5.56612461735667E-4	0.0	7.766685512590704E-5	2.958737338129792E-4
68-69	8.337413000640087	5.640093050809916E-4	0.0	7.766685512590704E-5	3.0327057715830364E-4
70-71	9.145372048460715	5.69556937589985E-4	0.0	7.766685512590704E-5	3.1251663133995925E-4
72-73	9.977853481181931	5.86199835116965E-4	0.0	7.766685512590704E-5	3.291595288669393E-4
74-75	10.828283354280577	6.083903651529384E-4	0.0	7.766685512590704E-5	3.3285795053960155E-4
76-77	11.699797929335071	6.250332626799185E-4	0.0	7.951606596223816E-5	3.421040047212572E-4
78-79	12.588471331739882	6.435253710432297E-4	0.0	8.136527679856927E-5	3.4765163723025054E-4
80-81	13.49245250144784	6.546206360612165E-4	0.0	8.136527679856927E-5	3.4765163723025054E-4
82-83	14.412342431980754	6.601682685702097E-4	0.0	8.321448763490039E-5	3.5135005890291277E-4
84-85	15.342765467437413	6.712635335881965E-4	0.0	8.506369847123152E-5	3.55048480575575E-4
86-87	16.28395090996152	6.860572202788454E-4	0.0	8.876212014389375E-5	3.661437455935617E-4
88	16.994406618014917	6.916048527878388E-4	0.0	9.2460541816556E-5	3.69842167266224E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	125030	0.0	81.002	8
GTGGTAT	126035	0.0	80.413445	6
AGTGGTA	127275	0.0	79.7343	5
GTATCAA	127260	0.0	79.38661	9
TGGTATC	127335	0.0	79.36238	7
AAGCAGT	130985	0.0	78.48936	1
GCAGTGG	135175	0.0	75.785545	3
CAGTGGT	138125	0.0	73.71079	4
AGCAGTG	142205	0.0	72.36375	2
CAACGCA	123155	0.0	43.216175	12-13
ATCAACG	124570	0.0	42.806618	10-11
ACGCAGA	127055	0.0	41.84708	14-15
AGAGTAC	126035	0.0	41.527557	18-19
TCAACGC	122705	0.0	41.174816	12-13
AACGCAG	125095	0.0	40.222755	14-15
GAGTACT	119315	0.0	40.018646	20-21
AGTACTT	128315	0.0	39.502346	20-21
TATCAAC	128970	0.0	39.19301	10-11
CGCAGAG	129045	0.0	38.883587	16-17
GTACTTT	124710	0.0	38.2334	22-23
>>END_MODULE