FastQCFastQC Report
Thu 26 May 2016
SRR1660656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27038561
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9175923.3936421394614897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310990.115017215598123No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT276110.10211712080387711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1253200.080.784188
GTGGTAT1262000.080.346
AGTGGTA1275200.079.559945
TGGTATC1276300.079.259487
GTATCAA1274450.079.190229
AAGCAGT1306800.078.151011
GCAGTGG1350400.075.5156253
CAGTGGT1377450.073.8519064
AGCAGTG1414650.072.278022
CAACGCA1231000.042.91040812-13
ATCAACG1247500.042.4369610-11
ACGCAGA1264800.041.76271414-15
AGAGTAC1278200.041.03069718-19
TCAACGC1227450.040.99071512-13
AACGCAG1245050.040.25554314-15
GAGTACT1205250.039.8680320-21
AGTACTT1293800.039.24566320-21
TATCAAC1294800.038.96010610-11
CGCAGAG1289450.038.80465316-17
GTACTTT1255550.038.15242822-23
Produced by FastQC (version 0.11.5)