##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660656_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27038561 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80610813571033 34.0 31.0 34.0 31.0 34.0 2 32.98840315503477 34.0 31.0 34.0 31.0 34.0 3 33.0418143184469 34.0 33.0 34.0 31.0 34.0 4 36.41292674562082 37.0 37.0 37.0 35.0 37.0 5 36.33974222962531 37.0 37.0 37.0 35.0 37.0 6 36.33293583930003 37.0 37.0 37.0 35.0 37.0 7 36.28223780104274 37.0 37.0 37.0 35.0 37.0 8 36.302228583836246 37.0 37.0 37.0 35.0 37.0 9 38.08812536288451 39.0 39.0 39.0 37.0 39.0 10-11 38.029465417926644 39.0 38.0 39.0 36.0 39.0 12-13 38.07279483179597 39.0 38.0 39.0 37.0 39.0 14-15 39.59345168184061 41.0 40.0 41.0 37.0 41.0 16-17 39.56833653240644 41.0 40.0 41.0 37.0 41.0 18-19 39.536284863680436 41.0 40.0 41.0 37.0 41.0 20-21 39.499755441866895 41.0 39.5 41.0 37.0 41.0 22-23 39.37771104016963 41.0 39.0 41.0 36.5 41.0 24-25 39.37385796899473 41.0 39.0 41.0 36.0 41.0 26-27 39.32374063841637 41.0 39.0 41.0 36.0 41.0 28-29 39.25162086103621 41.0 39.0 41.0 36.0 41.0 30-31 39.16813363329506 40.0 39.0 41.0 36.0 41.0 32-33 39.07609262194094 40.0 39.0 41.0 35.5 41.0 34-35 38.97782722238806 40.0 38.5 41.0 35.0 41.0 36-37 38.839229535920936 40.0 38.0 41.0 35.0 41.0 38-39 38.557197699981145 40.0 38.0 41.0 35.0 41.0 40-41 38.43971968404679 40.0 38.0 41.0 35.0 41.0 42-43 38.311577139774556 40.0 38.0 41.0 34.0 41.0 44-45 38.18479737142816 40.0 37.5 41.0 34.0 41.0 46-47 38.04731483306379 40.0 37.0 41.0 34.0 41.0 48-49 37.89015010821027 40.0 37.0 41.0 33.0 41.0 50-51 37.932531357715376 40.0 37.0 41.0 33.5 41.0 52-53 37.84133092363902 40.0 37.0 41.0 33.0 41.0 54-55 37.414856582049616 40.0 36.5 41.0 32.5 41.0 56-57 37.135760349820394 40.0 36.0 41.0 32.0 41.0 58-59 36.916579861627994 39.0 36.0 41.0 32.0 41.0 60-61 36.692355373497875 39.0 35.0 41.0 31.0 41.0 62-63 36.43551986734797 39.0 35.0 41.0 31.0 41.0 64-65 36.16352893188362 38.0 35.0 40.0 31.0 41.0 66-67 35.88451506350505 37.0 35.0 40.0 31.0 41.0 68-69 35.58773222066071 37.0 35.0 39.5 31.0 41.0 70-71 35.267296898677415 36.0 35.0 39.0 30.0 41.0 72-73 34.91673042437428 36.0 35.0 39.0 30.0 40.5 74-75 34.5686455540293 35.0 34.0 37.5 30.0 40.0 76-77 32.71763179260908 34.5 32.0 36.0 27.0 38.5 78-79 33.85788629801712 35.0 34.0 37.0 29.0 39.0 80-81 33.82543695650075 35.0 34.0 36.0 30.0 39.0 82-83 33.603053098868685 35.0 34.0 36.0 29.5 37.0 84-85 33.380479623157456 35.0 34.0 35.5 29.0 37.0 86-87 33.19428119713915 35.0 34.0 35.0 29.0 36.5 88-89 33.032723320593874 35.0 34.0 35.0 29.0 36.0 90-91 32.921687160052635 35.0 34.0 35.0 29.0 36.0 92-93 32.82778819109493 35.0 34.0 35.0 29.0 36.0 94-95 32.730270372006856 35.0 34.0 35.0 29.0 35.5 96-97 32.65742855176353 35.0 34.0 35.0 29.0 35.0 98-99 32.567064608208995 35.0 34.0 35.0 28.5 35.0 100 32.519514999337424 35.0 34.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37640434702231573 1101 2 0.32899711607000626 1101 3 0.30840299073280164 1101 4 0.22422763059152118 1101 5 0.22728908779421175 1101 6 0.2355274520549031 1101 7 0.2530923564668157 1101 8 0.2533216143903587 1101 9 0.31127189501642505 1101 10-11 0.32106534931966024 1101 12-13 0.3332776172076066 1101 14-15 0.4065382103608286 1101 16-17 0.4122920540107273 1101 18-19 0.40624756138836204 1101 20-21 0.4142244717593897 1101 22-23 0.4123830998551625 1101 24-25 0.41088588969556383 1101 26-27 0.4398572880146858 1101 28-29 0.4454983350473398 1101 30-31 0.47939816835889815 1101 32-33 0.4554908997864686 1101 34-35 0.4811336792028982 1101 36-37 0.5069167014298372 1101 38-39 0.5107581755468047 1101 40-41 0.5029800857313944 1101 42-43 0.5335837372362562 1101 44-45 0.5290797331113097 1101 46-47 0.4739626659916425 1101 48-49 0.47955888898904675 1101 50-51 0.45210960403890255 1101 52-53 0.48704535290737994 1101 54-55 0.3559198504336436 1101 56-57 0.3832245885774057 1101 58-59 0.4074212407927078 1101 60-61 0.3776022450068126 1101 62-63 0.3368019649667602 1101 64-65 0.3489620937214184 1101 66-67 0.3623701067277807 1101 68-69 0.378070904159884 1101 70-71 0.35601249214163744 1101 72-73 0.3759257942068075 1101 74-75 0.35989613027910394 1101 76-77 0.40799237771363295 1101 78-79 0.4562311219412294 1101 80-81 0.41154783300191156 1101 82-83 0.4104589190847676 1101 84-85 0.37069385097987606 1101 86-87 0.3982573035399142 1101 88-89 0.4078389077610467 1101 90-91 0.37619638693950463 1101 92-93 0.39160699707915114 1101 94-95 0.3777421437631858 1101 96-97 0.3595512233945968 1101 98-99 0.349538275321045 1101 100 0.3655053743191772 1102 1 0.3143437976734731 1102 2 0.25450377513541866 1102 3 0.28107045486841287 1102 4 0.20424780390492003 1102 5 0.22195012975122808 1102 6 0.22035284868235294 1102 7 0.22177181338077645 1102 8 0.21407325088110696 1102 9 0.27182882207132053 1102 10-11 0.2856080569661472 1102 12-13 0.29357777117986217 1102 14-15 0.38034447169055596 1102 16-17 0.3812672680809044 1102 18-19 0.39960363908969754 1102 20-21 0.4002579956668342 1102 22-23 0.3834527888593726 1102 24-25 0.38228866744069023 1102 26-27 0.40729102324489475 1102 28-29 0.4183382152467914 1102 30-31 0.4083375487550214 1102 32-33 0.41616352700567205 1102 34-35 0.4333296408666172 1102 36-37 0.4327514703379691 1102 38-39 0.44904789856715155 1102 40-41 0.4494555494119865 1102 42-43 0.4173727124721225 1102 44-45 0.4441013096979489 1102 46-47 0.44653719595805086 1102 48-49 0.43114975096428765 1102 50-51 0.4476949997659503 1102 52-53 0.4419192771207676 1102 54-55 0.34646398878130213 1102 56-57 0.3507750312569513 1102 58-59 0.3627531221226121 1102 60-61 0.27518217268993084 1102 62-63 0.28628838744368323 1102 64-65 0.31855974676942367 1102 66-67 0.28773596020584336 1102 68-69 0.2792774448249773 1102 70-71 0.3067719491797689 1102 72-73 0.3420005530468657 1102 74-75 0.36798308030446947 1102 76-77 0.3473518148695618 1102 78-79 0.39095193598851097 1102 80-81 0.37438504693031405 1102 82-83 0.35078235507985056 1102 84-85 0.36210931343259745 1102 86-87 0.3819435420200392 1102 88-89 0.4202061209182375 1102 90-91 0.397024464925849 1102 92-93 0.37104963173683814 1102 94-95 0.38264972099698724 1102 96-97 0.37990396323986175 1102 98-99 0.3653327819751979 1102 100 0.4058105253275386 1103 1 0.27548423004362377 1103 2 0.25239531889919675 1103 3 0.2297362079015599 1103 4 0.15332693948446519 1103 5 0.16404860748453132 1103 6 0.18075107550028946 1103 7 0.21162904086092027 1103 8 0.16708173561946893 1103 9 0.22183328779236433 1103 10-11 0.2583806211621109 1103 12-13 0.23993256601636404 1103 14-15 0.31810977066800916 1103 16-17 0.2938149504605221 1103 18-19 0.30641354860901515 1103 20-21 0.31974737754612903 1103 22-23 0.3263986121729374 1103 24-25 0.3215192387180892 1103 26-27 0.28928697506486145 1103 28-29 0.3446640907862175 1103 30-31 0.33916684915327977 1103 32-33 0.3077968046918258 1103 34-35 0.3536692640318222 1103 36-37 0.33409917822283575 1103 38-39 0.3691348405660122 1103 40-41 0.3229026563087771 1103 42-43 0.3478569611620088 1103 44-45 0.3231490362494185 1103 46-47 0.31910900795663366 1103 48-49 0.3121008157847598 1103 50-51 0.35073481982350785 1103 52-53 0.33729578701017005 1103 54-55 0.25274892619996336 1103 56-57 0.2502614976049031 1103 58-59 0.30065287397791707 1103 60-61 0.2533928350614545 1103 62-63 0.21566380709992217 1103 64-65 0.2105754941102873 1103 66-67 0.2298360503206922 1103 68-69 0.210966177495294 1103 70-71 0.23159348095919796 1103 72-73 0.2553510492494766 1103 74-75 0.2475409610878856 1103 76-77 0.15610280925034914 1103 78-79 0.3307724641015426 1103 80-81 0.31940699864134814 1103 82-83 0.31312418446015045 1103 84-85 0.32855877118991117 1103 86-87 0.32395556196253494 1103 88-89 0.3503827954877323 1103 90-91 0.3035026413374524 1103 92-93 0.2819609730698929 1103 94-95 0.2854801659064492 1103 96-97 0.30578099000861414 1103 98-99 0.28820210839631244 1103 100 0.28939678716874084 1104 1 0.22890055668442955 1104 2 0.20422227457462583 1104 3 0.18438886791142295 1104 4 0.14765670654712437 1104 5 0.14564597110820188 1104 6 0.16818469790598556 1104 7 0.18024038005533072 1104 8 0.13833386523680957 1104 9 0.19730572382810863 1104 10-11 0.2107563477963197 1104 12-13 0.18540670449252872 1104 14-15 0.21855264873683922 1104 16-17 0.2542811926889712 1104 18-19 0.2517855102619393 1104 20-21 0.2665777321353815 1104 22-23 0.2913271123398147 1104 24-25 0.30182457300613663 1104 26-27 0.2632572562435129 1104 28-29 0.2700681965288041 1104 30-31 0.2758645552472885 1104 32-33 0.28081031878277685 1104 34-35 0.2845337980584901 1104 36-37 0.2869567138098077 1104 38-39 0.26117052300267574 1104 40-41 0.25322493243844235 1104 42-43 0.25263458489218493 1104 44-45 0.28365738914701666 1104 46-47 0.26188878602976473 1104 48-49 0.3253391477086893 1104 50-51 0.2929273602808067 1104 52-53 0.2901732351905366 1104 54-55 0.18989260721015455 1104 56-57 0.2118596015146963 1104 58-59 0.22733538810668108 1104 60-61 0.1870091330020074 1104 62-63 0.16136442787070138 1104 64-65 0.1723131813217833 1104 66-67 0.16131704348759257 1104 68-69 0.14119134126549682 1104 70-71 0.16255648775148757 1104 72-73 0.18554928073046995 1104 74-75 0.20075463787365067 1104 76-77 0.143702866560794 1104 78-79 0.23074102952468678 1104 80-81 0.23807290006797643 1104 82-83 0.2405693816893617 1104 84-85 0.2738077277321196 1104 86-87 0.2833447452991962 1104 88-89 0.2817337394546229 1104 90-91 0.2658886718568283 1104 92-93 0.27708618368985327 1104 94-95 0.3137490701161312 1104 96-97 0.3196532543171955 1104 98-99 0.2969965900845253 1104 100 0.303448409302419 1105 1 0.17508455819697133 1105 2 0.13557153652450182 1105 3 0.10423618269314971 1105 4 0.11180737608547986 1105 5 0.07320301315603217 1105 6 0.08674362357923826 1105 7 0.11946564094505163 1105 8 0.11503883674797777 1105 9 0.15433042442751344 1105 10-11 0.14235578721626752 1105 12-13 0.12009592247107292 1105 14-15 0.1718702663054117 1105 16-17 0.1749867911932057 1105 18-19 0.21487760682008883 1105 20-21 0.1996176685243043 1105 22-23 0.18834601558310737 1105 24-25 0.19182640194809153 1105 26-27 0.19412228164517842 1105 28-29 0.1684315445113711 1105 30-31 0.17452703205658793 1105 32-33 0.19107612792712558 1105 34-35 0.2148291110864875 1105 36-37 0.18006066905154938 1105 38-39 0.1988310558974291 1105 40-41 0.21899471233867018 1105 42-43 0.1999553984515643 1105 44-45 0.22001751726400443 1105 46-47 0.18955018124471934 1105 48-49 0.18138940617608768 1105 50-51 0.18446717166444415 1105 52-53 0.21921644871969903 1105 54-55 0.15784026953902242 1105 56-57 0.19800334624225968 1105 58-59 0.15428010762118305 1105 60-61 0.11825352561081104 1105 62-63 0.07266473392159156 1105 64-65 0.1457912053482886 1105 66-67 0.1439458466361927 1105 68-69 0.082227415221098 1105 70-71 0.08784864625121003 1105 72-73 0.1329894354279375 1105 74-75 0.15584172813635888 1105 76-77 0.08356078551693003 1105 78-79 0.21187739656075877 1105 80-81 0.2073840694401312 1105 82-83 0.2431223126697546 1105 84-85 0.264804504676583 1105 86-87 0.27248433266884575 1105 88-89 0.2740679618195827 1105 90-91 0.2727809000248911 1105 92-93 0.2108394475308657 1105 94-95 0.2545962192438651 1105 96-97 0.2519520647058826 1105 98-99 0.2612537127576928 1105 100 0.2789564697733198 1106 1 0.21115533765247818 1106 2 0.21089656174966365 1106 3 0.1972957045117667 1106 4 0.13497775017334845 1106 5 0.1218498011784348 1106 6 0.13850141000046534 1106 7 0.15166324772724948 1106 8 0.16788571477377445 1106 9 0.19950753301741742 1106 10-11 0.20022690541725296 1106 12-13 0.18372485556339058 1106 14-15 0.24675640211146543 1106 16-17 0.26070617020539544 1106 18-19 0.26366706093826764 1106 20-21 0.2383398012453668 1106 22-23 0.24879943856856812 1106 24-25 0.2679708562965075 1106 26-27 0.23344766045637044 1106 28-29 0.2530682141437879 1106 30-31 0.22906103952209378 1106 32-33 0.2802866099723289 1106 34-35 0.2700541085473489 1106 36-37 0.2620627985356876 1106 38-39 0.27044847864193144 1106 40-41 0.27172815126264993 1106 42-43 0.3021906322782897 1106 44-45 0.3218948179459318 1106 46-47 0.34174513909800197 1106 48-49 0.3118872914789321 1106 50-51 0.2744832540799038 1106 52-53 0.27056519565687864 1106 54-55 0.24188900412848113 1106 56-57 0.2377168245478387 1106 58-59 0.24977570307165564 1106 60-61 0.22848402143924318 1106 62-63 0.19774403486252368 1106 64-65 0.21467495491420863 1106 66-67 0.2067284481793763 1106 68-69 0.2243808897997397 1106 70-71 0.1917053427167943 1106 72-73 0.21563043460345455 1106 74-75 0.20463582959287407 1106 76-77 0.13311016574842682 1106 78-79 0.2828308333120475 1106 80-81 0.31197400982487267 1106 82-83 0.3275931172687123 1106 84-85 0.34297965441247413 1106 86-87 0.3455262733818003 1106 88-89 0.37824519698608583 1106 90-91 0.32241574813439655 1106 92-93 0.2891685482146684 1106 94-95 0.2923715435226484 1106 96-97 0.2759933131434167 1106 98-99 0.3109334270086279 1106 100 0.35498794436033165 1107 1 0.16124977422715148 1107 2 0.15712500830044718 1107 3 0.14298722775166084 1107 4 0.11699231110093677 1107 5 0.11166664661116954 1107 6 0.09986679372853757 1107 7 0.11743311767092734 1107 8 0.11869622467941099 1107 9 0.12941464471736452 1107 10-11 0.1485897125602662 1107 12-13 0.1305356142715155 1107 14-15 0.17778999116182348 1107 16-17 0.13982519236594015 1107 18-19 0.18032077681615277 1107 20-21 0.15392589387885636 1107 22-23 0.16057286625814982 1107 24-25 0.1579949816360866 1107 26-27 0.15849134547139698 1107 28-29 0.19150434206542855 1107 30-31 0.15997566058235435 1107 32-33 0.15456458124309336 1107 34-35 0.1921834259016748 1107 36-37 0.17296498101870128 1107 38-39 0.13670789429512809 1107 40-41 0.16375636803727645 1107 42-43 0.12987358773062851 1107 44-45 0.13074354042745284 1107 46-47 0.17318152413960775 1107 48-49 0.17527805983843336 1107 50-51 0.1761629567115719 1107 52-53 0.16570199587934553 1107 54-55 0.10231761061508848 1107 56-57 0.08740291474059347 1107 58-59 0.07219876273328651 1107 60-61 0.01743335200827545 1107 62-63 0.04974823459428279 1107 64-65 0.0627633002482284 1107 66-67 0.07432059484954578 1107 68-69 0.006383244140792499 1107 70-71 0.022976337682827364 1107 72-73 0.031616477676827515 1107 74-75 0.06493300660038415 1107 76-77 -0.011412279014038518 1107 78-79 0.19234757116931434 1107 80-81 0.17865374012009028 1107 82-83 0.15718975850291628 1107 84-85 0.2138320478136464 1107 86-87 0.207119795184461 1107 88-89 0.2023594147877006 1107 90-91 0.22921601399618652 1107 92-93 0.16831805553129442 1107 94-95 0.17935122727311636 1107 96-97 0.1493110590927813 1107 98-99 0.19822966131166453 1107 100 0.23201028323882156 1108 1 0.04402739855806459 1108 2 0.023671535089341944 1108 3 0.014410816703623652 1108 4 0.031242337876513204 1108 5 0.04241525181845418 1108 6 0.037426705899619606 1108 7 0.026876026705828338 1108 8 0.03858778413557218 1108 9 0.03616755451691489 1108 10-11 0.04390873462735101 1108 12-13 0.024460748037604674 1108 14-15 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-0.5603154058854258 2316 52-53 -0.5282755142348563 2316 54-55 -0.4607412484929725 2316 56-57 -0.4737837860695393 2316 58-59 -0.4640070727855701 2316 60-61 -0.4059129508177364 2316 62-63 -0.3885216102941129 2316 64-65 -0.40386088630746997 2316 66-67 -0.39856476424971987 2316 68-69 -0.38896947870910026 2316 70-71 -0.40864286580527676 2316 72-73 -0.44238801325336397 2316 74-75 -0.43194175427954207 2316 76-77 -0.48466779065994103 2316 78-79 -0.6601230522680765 2316 80-81 -0.6432800328583497 2316 82-83 -0.6060369807586667 2316 84-85 -0.6090282470483643 2316 86-87 -0.6458436603004074 2316 88-89 -0.6966170278907597 2316 90-91 -0.6046878793258443 2316 92-93 -0.5237973806118461 2316 94-95 -0.5180893005251548 2316 96-97 -0.5739978579700065 2316 98-99 -1.331402552201233 2316 100 -0.8692119849550686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 302.0 8 487.0 9 201.0 10 56.0 11 63.0 12 98.0 13 356.0 14 1415.0 15 4441.0 16 10735.0 17 17489.0 18 22069.0 19 26696.0 20 34630.0 21 49280.0 22 75079.0 23 126795.0 24 219065.0 25 305461.0 26 321095.0 27 277331.0 28 254431.0 29 273501.0 30 320312.0 31 389757.0 32 482271.0 33 628056.0 34 887242.0 35 1474966.0 36 2977710.0 37 6816214.0 38 8871928.0 39 2166655.0 40 2370.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.08591603934947 30.813172065404338 28.407404042331535 23.693507852914657 2 22.377652013017528 32.09058260565488 25.714732923675854 19.817032457651734 3 25.02522231120214 25.16125395874433 26.979730910975626 22.833792819077907 4 20.881555050211436 27.56122265530329 24.470015249702083 27.08720704478319 5 22.896329431140956 29.98823051271109 26.121068351233635 20.994371704914325 6 27.282960805495527 27.187334414727175 25.290953908382917 20.238750871394377 7 22.3498617400534 25.717222895108954 29.873424107148306 22.05949125768934 8 26.272204352886973 26.757089624703028 25.191340619051434 21.779365403358558 9 25.396011274416562 25.46587076139148 26.88783622767499 22.250281736516968 10-11 21.875102746777095 28.641165482142338 27.68791763733284 21.795814133747722 12-13 21.733443950659947 26.689240229907206 27.738819014813693 23.83849680461915 14-15 21.747597810401224 30.51140184568254 25.880389862463467 21.860610481452767 16-17 23.829095416727245 26.368341125846158 25.975994062701783 23.826569394724814 18-19 21.777738377137943 28.35516666285728 26.043751427132843 23.82334353287193 20-21 23.770104536553674 28.461740186486345 25.996049220949875 21.77210605601011 22-23 23.72335267375684 26.5104706794347 27.933354685713184 21.832821961095274 24-25 21.90141325478657 28.17923798956448 25.891713247606024 24.027635508042923 26-27 21.957143791321556 26.27640037614008 29.90211355394379 21.864342278594577 28-29 22.011883406732434 26.35444082449847 29.820375584359166 21.813300184409933 30-31 21.91078041339762 26.282006802670765 29.871826690200336 21.935386093731275 32-33 22.022276418629488 26.443744668653185 29.635365236266797 21.89861367645053 34-35 22.020350326361086 26.566556882225434 29.552982551293955 21.86011024011953 36-37 21.965605604448193 26.50948722363126 29.63589318253067 21.889013989389877 38-39 22.01875564097105 26.514445315355538 29.646982503897323 21.819816539776085 40-41 22.016688176007754 26.58135641163748 29.62457152552873 21.777383886826033 42-43 22.04225277605843 26.596236371629523 29.54821085303711 21.81329999927494 44-45 22.080512866426126 26.69007700974837 29.488706144976483 21.740703978849023 46-47 22.120950487198588 26.78367736070714 29.381860831956786 21.71351132013749 48-49 22.139409788014692 26.810717615493125 29.280586782993456 21.769285813498723 50-51 22.192989739183904 26.90355162831739 29.151244659699394 21.752213972799314 52-53 22.287773190104 27.046898614809905 28.895042560111367 21.770285634974726 54-55 22.45439941137885 27.239817575430042 28.382740666502666 21.923042346688437 56-57 22.73816987259425 27.60316319743116 27.64023807819427 22.018428851780318 58-59 22.80521431242566 27.912346183288616 27.237360993016058 22.045078511269665 60-61 22.795942048535064 28.0618894803406 27.049296101598458 22.092872369525878 62-63 22.752077153754755 28.18268761601763 26.945460488077433 22.11977474215018 64-65 22.751284427798492 28.253212614725403 26.86819317296655 22.127309784509556 66-67 22.679168833441658 28.15510847903704 26.95547486990744 22.210247817613865 68-69 22.620345657981904 28.111053786896466 27.034567833880867 22.23403272124076 70-71 22.53905555815097 28.06128774573495 27.095744410210678 22.3039122859034 72-73 22.48270149132611 28.019371140665967 27.065484841687127 22.4324425263208 74-75 22.480575205630338 28.07128597310189 26.94176734370644 22.50637147756133 76-77 22.479672091745247 28.14424036465453 26.794490633536235 22.581596910063993 78-79 22.483090245400884 28.20095819785125 26.596337425012496 22.719614131735373 80-81 22.469638405246727 28.291578569913455 26.404187811274426 22.834595213565393 82-83 22.439953429953043 28.337519261256844 26.239355905901196 22.983171402888917 84-85 22.397111121314897 28.302857372904633 26.142519117288547 23.15751238849192 86-87 22.367832142097964 28.295066509370155 26.063022347255625 23.274079001276256 88-89 22.332374268854878 28.25529628590419 26.007826287174073 23.404503158066852 90-91 22.29670248302576 28.134006624370723 26.00311091112725 23.566179981476264 92-93 22.270401780356273 28.075359637626335 26.0121217792393 23.64211680277809 94-95 22.216316954296055 28.02989111012645 26.018875541688903 23.734916393888593 96-97 22.152619197173458 27.93791086344639 26.04637617813856 23.863093761241593 98-99 22.133595752813573 27.89563888189773 26.028786748790182 23.941978616498513 100 22.048899997666254 27.892872862750345 26.026246169337696 24.031980970245705 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 35905.0 1 23935.5 2 9725.5 3 6915.5 4 6270.5 5 6083.0 6 6069.5 7 6398.0 8 6553.5 9 6801.5 10 7402.0 11 11065.5 12 94529.0 13 109601.5 14 42670.0 15 43303.0 16 48731.5 17 53866.0 18 59044.5 19 65179.5 20 73412.0 21 86078.0 22 103037.5 23 122584.0 24 142310.5 25 164345.0 26 188431.5 27 216425.0 28 257406.5 29 301933.5 30 337261.5 31 374445.5 32 415951.0 33 450315.5 34 483271.5 35 531759.5 36 586382.0 37 622815.0 38 657162.0 39 697041.5 40 730519.5 41 769201.0 42 818291.0 43 849513.0 44 866093.5 45 895509.0 46 925543.0 47 950796.5 48 971236.0 49 988809.5 50 1007382.5 51 1021285.5 52 1021703.0 53 1002330.5 54 962349.5 55 907487.0 56 841496.0 57 766677.5 58 680831.0 59 580939.0 60 478367.0 61 384692.5 62 301739.0 63 226801.5 64 166998.5 65 122472.5 66 88135.0 67 64812.0 68 48282.0 69 35728.0 70 27426.0 71 22569.5 72 18868.0 73 14307.5 74 10897.5 75 9284.0 76 6771.5 77 3452.0 78 1771.5 79 1130.0 80 636.5 81 379.5 82 226.5 83 125.0 84 83.5 85 52.0 86 41.5 87 24.0 88 10.0 89 5.0 90 3.5 91 3.0 92 1.5 93 1.0 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10762037225279851 2 0.0747709909562125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.069689988309659E-4 20-21 0.0022282990577789995 22-23 7.433827562051103E-4 24-25 8.524861955486461E-4 26-27 0.0067496195526085875 28-29 0.006603531896538429 30-31 0.007844352367716611 32-33 0.007853598421898266 34-35 0.007186033309982732 36-37 0.01096397104860721 38-39 0.013515882002744155 40-41 0.016901787044066436 42-43 0.023481279199732558 44-45 0.011899671731790755 46-47 0.018778736042942522 48-49 0.01618429323956996 50-51 0.018815720259669145 52-53 0.02121784513606327 54-55 0.1122729867170076 56-57 0.10488538942586478 58-59 0.10206904132213249 60-61 0.14488381981570692 62-63 0.09503279408989258 64-65 0.0736540676110685 66-67 0.08359912348885727 68-69 0.07654993178076304 70-71 0.05325172445382726 72-73 0.03164924346380712 74-75 0.020655685041818608 76-77 0.01271517371061278 78-79 0.008842926219335415 80-81 0.005643791472482578 82-83 5.603108834083293E-4 84-85 0.0024612996231567204 86-87 0.0016772342285523256 88-89 0.00214693378098043 90-91 0.0030438010366010235 92-93 0.004719186054317018 94-95 0.0020544732391638743 96-97 0.001126169399325652 98-99 0.001575527632554114 100 0.0017826392462231995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7038561E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.5195774668194 #Duplication Level Percentage of deduplicated Percentage of total 1 76.73863954977824 36.46587726784018 2 11.228383161479348 10.671360469380968 3 4.277981007315261 6.0986354963609894 4 2.1857086681112516 4.154558094968451 5 1.33917039140267 3.1818405577765017 6 0.8384099034930436 2.3904530614791755 7 0.5846385274038414 1.9447243055137837 8 0.4176851206652939 1.5878576358553793 9 0.33610664715010485 1.4374481270726107 >10 1.8233469578514567 15.788952337368881 >50 0.14151198205809914 4.584260038317471 >100 0.0874662745201854 7.518542396549697 >500 5.072016450046331E-4 0.15809912760072517 >1k 4.0580822180475844E-4 0.3281175474890119 >5k 7.75978086463592E-6 0.029248576408421382 >10k+ 3.103912345854319E-5 3.6600249600175454 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 917592 3.3936421394614897 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31099 0.115017215598123 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 27611 0.10211712080387711 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.004737678162680329 1.84921083633112E-5 0.0 0.0 7.39684334532448E-6 2 0.004752471849370978 2.2190530035973438E-5 0.0 3.69842167266224E-6 7.39684334532448E-6 3 0.004759868692716302 3.328579505396016E-5 0.0 1.1095265017986719E-5 1.1095265017986719E-5 4 0.004759868692716302 3.69842167266224E-5 0.0 2.2190530035973438E-5 2.2190530035973438E-5 5 0.004759868692716302 4.8079481744609114E-5 0.0 2.2190530035973438E-5 2.958737338129792E-5 6 0.004759868692716302 5.177790341727135E-5 0.0 2.2190530035973438E-5 5.547632508993359E-5 7 0.004759868692716302 5.917474676259584E-5 0.0 2.2190530035973438E-5 6.287316843525807E-5 8 0.004759868692716302 5.917474676259584E-5 0.0 2.2190530035973438E-5 6.657159010792032E-5 9 0.004763567114388965 6.657159010792032E-5 0.0 2.2190530035973438E-5 6.657159010792032E-5 10-11 0.004770963957734289 7.211922261691366E-5 0.0 2.2190530035973438E-5 6.657159010792032E-5 12-13 0.004782059222752276 8.50636984712315E-5 0.0 2.2190530035973438E-5 7.581764428957591E-5 14-15 0.004802400541951918 1.0355580683454272E-4 0.0 2.2190530035973438E-5 9.2460541816556E-5 16-17 0.004819043439478898 1.0910343934353607E-4 0.0 2.2190530035973438E-5 1.1095265017986718E-4 18-19 0.004846781602023865 1.2204791519785391E-4 0.0 2.2190530035973438E-5 1.1465107185252943E-4 20-21 0.004893011872932142 1.3314318021584063E-4 0.0 2.2190530035973438E-5 1.1465107185252943E-4 22-23 0.005142655335836844 1.5348449941548294E-4 0.0 2.2190530035973438E-5 1.1465107185252943E-4 24-25 0.006392721861196681 1.7567502945145638E-4 0.0 2.4039740872304557E-5 1.2204791519785391E-4 26-27 0.01094732815108023 1.8861950530577422E-4 0.0 2.5888951708635677E-5 1.294447585431784E-4 28-29 0.024183979317538386 1.9786555948742983E-4 0.0 2.5888951708635677E-5 1.3314318021584063E-4 30-31 0.05372882084960069 2.145084570144099E-4 0.0 2.5888951708635677E-5 1.4238923439749624E-4 32-33 0.10955464678760085 2.256037220323966E-4 0.0 2.7738162544966796E-5 1.5163528857915185E-4 34-35 0.19901207020595513 2.4039740872304558E-4 0.0 2.958737338129792E-5 1.6088134276080743E-4 36-37 0.32797048629917847 2.533418845773634E-4 0.0 3.328579505396016E-5 1.7382581861512527E-4 38-39 0.4981441135125497 2.5704030625002566E-4 0.0 3.328579505396016E-5 1.775242402877875E-4 40-41 0.714625678489325 2.6628636043168126E-4 0.0 3.69842167266224E-5 1.84921083633112E-4 42-43 0.9759376617712755 2.792308362859991E-4 0.0 4.0682638399284636E-5 1.84921083633112E-4 44-45 1.282277928917889 2.8847689046765466E-4 0.0 4.0682638399284636E-5 1.9046871614210534E-4 46-47 1.6386615397172948 2.958737338129792E-4 0.0 4.4381060071946875E-5 1.9601634865109872E-4 48-49 2.0442729921906717 3.1621505301262154E-4 0.0 4.8079481744609114E-5 2.0341319199642318E-4 50-51 2.4970134320387833 3.4210400472125716E-4 3.69842167266224E-6 4.8079481744609114E-5 2.1635766785074102E-4 52-53 2.9929089051743545 3.5319926973924386E-4 3.69842167266224E-6 4.8079481744609114E-5 2.2190530035973437E-4 54-55 3.531754149194552 3.69842167266224E-4 3.69842167266224E-6 4.8079481744609114E-5 2.3115135454138998E-4 56-57 4.1130757661252755 3.9758032981119075E-4 3.69842167266224E-6 5.177790341727135E-5 2.3854819788671447E-4 58-59 4.7367017793587465 4.1607243817450197E-4 3.69842167266224E-6 5.547632508993359E-5 2.4594504123203896E-4 60-61 5.39966050708098 4.345645465378132E-4 3.69842167266224E-6 5.547632508993359E-5 2.533418845773634E-4 62-63 6.098871164038648 4.47509022392131E-4 3.69842167266224E-6 6.47223792715892E-5 2.588895170863568E-4 64-65 6.828738038241014 4.7709639577342894E-4 3.69842167266224E-6 6.657159010792032E-5 2.755324146133368E-4 66-67 7.588051745801117 4.8079481744609117E-4 3.69842167266224E-6 7.211922261691366E-5 2.8662767963132357E-4 68-69 8.376076300806098 4.8634244995508454E-4 3.69842167266224E-6 7.39684334532448E-5 2.9402452297664803E-4 70-71 9.189514560334775 4.974377149730713E-4 3.69842167266224E-6 7.39684334532448E-5 3.014213663219725E-4 72-73 10.027489998450731 5.196282450090446E-4 3.69842167266224E-6 7.39684334532448E-5 3.1251663133995925E-4 74-75 10.884586276614352 5.381203533723559E-4 3.69842167266224E-6 7.39684334532448E-5 3.254611071942771E-4 76-77 11.762103390043574 5.547632508993359E-4 3.69842167266224E-6 7.581764428957592E-5 3.439532155575883E-4 78-79 12.65824390580549 5.732553592626471E-4 3.69842167266224E-6 7.766685512590704E-5 3.495008480665817E-4 80-81 13.569675545973027 5.843506242806339E-4 3.69842167266224E-6 7.766685512590704E-5 3.624453239208995E-4 82-83 14.497306273066824 5.991443109712828E-4 3.69842167266224E-6 7.766685512590704E-5 3.69842167266224E-4 84-85 15.435612494318761 6.120887868256007E-4 3.69842167266224E-6 7.766685512590704E-5 3.69842167266224E-4 86-87 16.385901601790124 6.250332626799185E-4 3.69842167266224E-6 7.766685512590704E-5 3.7908822144787954E-4 88 17.104834092317265 6.361285276979052E-4 3.69842167266224E-6 8.136527679856927E-5 3.809374322842107E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 125320 0.0 80.78418 8 GTGGTAT 126200 0.0 80.34 6 AGTGGTA 127520 0.0 79.55994 5 TGGTATC 127630 0.0 79.25948 7 GTATCAA 127445 0.0 79.19022 9 AAGCAGT 130680 0.0 78.15101 1 GCAGTGG 135040 0.0 75.515625 3 CAGTGGT 137745 0.0 73.851906 4 AGCAGTG 141465 0.0 72.27802 2 CAACGCA 123100 0.0 42.910408 12-13 ATCAACG 124750 0.0 42.43696 10-11 ACGCAGA 126480 0.0 41.762714 14-15 AGAGTAC 127820 0.0 41.030697 18-19 TCAACGC 122745 0.0 40.990715 12-13 AACGCAG 124505 0.0 40.255543 14-15 GAGTACT 120525 0.0 39.86803 20-21 AGTACTT 129380 0.0 39.245663 20-21 TATCAAC 129480 0.0 38.960106 10-11 CGCAGAG 128945 0.0 38.804653 16-17 GTACTTT 125555 0.0 38.152428 22-23 >>END_MODULE