FastQCFastQC Report
Thu 26 May 2016
SRR1660655_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660655_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24584691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7912713.2185517401866064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426770.17359176895898346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1114950.079.996358
GTGGTAT1115600.079.934786
AGTGGTA1125000.079.345395
TGGTATC1129650.078.797137
GTATCAA1130600.078.6831059
AAGCAGT1172650.076.977781
GCAGTGG1212350.074.436623
CAGTGGT1216000.073.679564
AGCAGTG1280000.070.7393652
CAACGCA1101550.042.4420312-13
ATCAACG1118450.041.83209210-11
ACGCAGA1139450.041.0073114-15
AGAGTAC1131900.040.6680418-19
TCAACGC1099950.040.42567412-13
GAGTACT1041250.040.06135620-21
AGTACTT1101800.039.86842720-21
AACGCAG1120050.039.62006414-15
TATCAAC1151000.038.6110-11
GTACTTT1088750.038.19566722-23
CGCAGAG1160550.038.1167516-17
Produced by FastQC (version 0.11.5)