##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660655_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24584691 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38301176939747 34.0 31.0 34.0 31.0 34.0 2 32.54110405536519 34.0 31.0 34.0 31.0 34.0 3 32.564000092577935 34.0 31.0 34.0 31.0 34.0 4 35.92293427645684 37.0 37.0 37.0 35.0 37.0 5 35.918042085621494 37.0 37.0 37.0 35.0 37.0 6 35.930994455045216 37.0 37.0 37.0 35.0 37.0 7 35.89557643819888 37.0 36.0 37.0 35.0 37.0 8 35.909379906381574 37.0 37.0 37.0 35.0 37.0 9 37.63865146810265 39.0 38.0 39.0 35.0 39.0 10-11 37.58139319302407 39.0 38.0 39.0 35.0 39.0 12-13 37.6249094202567 39.0 38.0 39.0 35.0 39.0 14-15 39.10279748889258 41.0 39.0 41.0 36.0 41.0 16-17 39.0604386282504 41.0 39.0 41.0 36.0 41.0 18-19 39.02412737259948 41.0 39.0 41.0 36.0 41.0 20-21 38.960654701741014 41.0 39.0 41.0 35.5 41.0 22-23 38.83724222932068 40.5 39.0 41.0 35.0 41.0 24-25 38.819636272833364 40.0 39.0 41.0 35.0 41.0 26-27 38.77227657650853 40.0 39.0 41.0 35.0 41.0 28-29 38.686582475248514 40.0 38.5 41.0 35.0 41.0 30-31 38.590571120051905 40.0 38.0 41.0 34.0 41.0 32-33 38.47918808497532 40.0 38.0 41.0 34.0 41.0 34-35 38.37047002136411 40.0 38.0 41.0 34.0 41.0 36-37 38.220712780160625 40.0 38.0 41.0 33.5 41.0 38-39 37.91438442321687 40.0 37.0 41.0 33.0 41.0 40-41 37.771736057207306 40.0 37.0 41.0 33.0 41.0 42-43 37.62190454213966 40.0 37.0 41.0 33.0 41.0 44-45 37.47279630238184 40.0 36.5 41.0 32.5 41.0 46-47 37.29965493566708 40.0 36.0 41.0 32.0 41.0 48-49 37.3131855714599 40.0 36.0 41.0 32.0 41.0 50-51 36.34543932238155 38.5 35.0 40.0 31.0 40.5 52-53 36.55891721396864 39.0 35.5 39.5 31.0 40.5 54-55 37.041860115305084 40.0 36.0 41.0 31.0 41.0 56-57 36.821225290161266 40.0 35.5 41.0 31.0 41.0 58-59 36.60488197716213 39.0 35.0 41.0 30.0 41.0 60-61 36.3753670526101 39.0 35.0 41.0 30.0 41.0 62-63 36.101295741321294 38.5 35.0 41.0 29.5 41.0 64-65 35.80324513739058 37.5 35.0 40.5 29.0 41.0 66-67 35.4889253641626 37.0 35.0 40.0 29.0 41.0 68-69 35.17584717660271 36.5 35.0 39.5 29.0 41.0 70-71 34.83602114421532 36.0 34.5 39.0 29.0 41.0 72-73 34.480552063883984 35.5 34.0 39.0 28.0 41.0 74-75 34.12483382443163 35.0 34.0 37.5 28.0 40.0 76-77 33.77649896026759 35.0 34.0 37.0 27.5 39.0 78-79 33.446315900411356 35.0 34.0 36.5 27.0 39.0 80-81 33.1401180311764 35.0 34.0 36.0 26.5 38.0 82-83 32.88546093583198 35.0 34.0 36.0 26.0 37.0 84-85 32.65848134922664 35.0 33.0 35.0 26.0 37.0 86-87 32.46240912688307 35.0 33.0 35.0 25.0 36.5 88-89 32.283835497464665 35.0 33.0 35.0 25.0 36.0 90-91 32.16201387277961 35.0 33.0 35.0 25.0 36.0 92-93 32.04104243164984 35.0 33.0 35.0 24.5 36.0 94-95 31.939465987186903 35.0 33.0 35.0 24.0 35.0 96-97 31.838937511966286 35.0 33.0 35.0 24.0 35.0 98-99 31.592883005932432 35.0 33.0 35.0 23.0 35.0 100 31.52416375703075 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47650860915860704 1101 2 0.47096661595197276 1101 3 0.47859542554527934 1101 4 0.4199272049274114 1101 5 0.3968386881887511 1101 6 0.39192507800954957 1101 7 0.3883535992104825 1101 8 0.40749660444255653 1101 9 0.5143983319094048 1101 10-11 0.4979650945574363 1101 12-13 0.47019914664343077 1101 14-15 0.6046210414701108 1101 16-17 0.6198392662821632 1101 18-19 0.6043788788688857 1101 20-21 0.6286707768171667 1101 22-23 0.628609605448716 1101 24-25 0.6508956496383576 1101 26-27 0.6197109404792016 1101 28-29 0.6255387732081701 1101 30-31 0.6596275935725657 1101 32-33 0.6500010821073587 1101 34-35 0.6605560962447541 1101 36-37 0.6696907192320367 1101 38-39 0.7023081923827945 1101 40-41 0.6954955832875171 1101 42-43 0.7120704680225387 1101 44-45 0.7004650041464018 1101 46-47 0.6773701604520781 1101 48-49 0.7105409846288566 1101 50-51 0.6381099200840268 1101 52-53 0.7132155336425612 1101 54-55 0.7673003026229566 1101 56-57 0.7393184792489151 1101 58-59 0.6891169580644245 1101 60-61 0.7019785652914194 1101 62-63 0.6680598020006485 1101 64-65 0.6584955414025728 1101 66-67 0.6236731239110469 1101 68-69 0.6514843320675681 1101 70-71 0.6365205963238338 1101 72-73 0.5572330097221112 1101 74-75 0.5949126182415583 1101 76-77 0.5708702819474283 1101 78-79 0.5549701477675697 1101 80-81 0.5531995808130858 1101 82-83 0.5152310423731947 1101 84-85 0.5105943112233149 1101 86-87 0.5420643916461856 1101 88-89 0.5625951081691554 1101 90-91 0.5348246426826861 1101 92-93 0.5320457006332973 1101 94-95 0.5166142382441627 1101 96-97 0.46953568825650294 1101 98-99 0.2569990935620936 1101 100 0.35791408822137427 1102 1 0.4764984358098161 1102 2 0.4513948397362668 1102 3 0.4673661197525547 1102 4 0.40566972658647416 1102 5 0.41428474192720444 1102 6 0.36949612136150023 1102 7 0.39170419121827393 1102 8 0.41241048995647844 1102 9 0.48787769264278324 1102 10-11 0.5373680382687311 1102 12-13 0.47803122772736373 1102 14-15 0.5744713948367632 1102 16-17 0.6188806964257552 1102 18-19 0.610323283855216 1102 20-21 0.5814695495003761 1102 22-23 0.6113849405829868 1102 24-25 0.5984791295140752 1102 26-27 0.5842608834656104 1102 28-29 0.5879635510471459 1102 30-31 0.5762250951927541 1102 32-33 0.588209929212482 1102 34-35 0.5954082664650286 1102 36-37 0.5900382394957333 1102 38-39 0.6201356127753215 1102 40-41 0.6259895549378456 1102 42-43 0.6335134533192885 1102 44-45 0.5976040005978618 1102 46-47 0.6233731403033005 1102 48-49 0.598105445749276 1102 50-51 0.6071740973530453 1102 52-53 0.6501095398839425 1102 54-55 0.7136425507628488 1102 56-57 0.7041262163909394 1102 58-59 0.6630276754411568 1102 60-61 0.6153656391941595 1102 62-63 0.6414876014073982 1102 64-65 0.6312214670856022 1102 66-67 0.6007820077491957 1102 68-69 0.5854623346256105 1102 70-71 0.5607107351645908 1102 72-73 0.5245232519275902 1102 74-75 0.5184378667671936 1102 76-77 0.5243987324576551 1102 78-79 0.5038641595988835 1102 80-81 0.5105277812552487 1102 82-83 0.49046561771103825 1102 84-85 0.5082360033668181 1102 86-87 0.5057114478479647 1102 88-89 0.5316632824079974 1102 90-91 0.4887796875777255 1102 92-93 0.5307387081379815 1102 94-95 0.5261636313456783 1102 96-97 0.5166616386151013 1102 98-99 0.31105154723199036 1102 100 0.3514456767480283 1103 1 0.42449077363586696 1103 2 0.4037887144655272 1103 3 0.39413534033694475 1103 4 0.3630756268223436 1103 5 0.4031803070494533 1103 6 0.33468196097616953 1103 7 0.38021115801247163 1103 8 0.38529072878758797 1103 9 0.45298711870690767 1103 10-11 0.4485960002054057 1103 12-13 0.43113495650848677 1103 14-15 0.5263364612285812 1103 16-17 0.5438848993608048 1103 18-19 0.5280760951331445 1103 20-21 0.5514333791462249 1103 22-23 0.5627326277767111 1103 24-25 0.5601291800415282 1103 26-27 0.564045774816968 1103 28-29 0.5682044098984065 1103 30-31 0.5717602805319615 1103 32-33 0.5695711574693192 1103 34-35 0.5553302890681451 1103 36-37 0.539058454703067 1103 38-39 0.569770505414958 1103 40-41 0.6091561507410646 1103 42-43 0.5972325854361529 1103 44-45 0.597054683318909 1103 46-47 0.6060184552628911 1103 48-49 0.5444881258073551 1103 50-51 0.49920513589965054 1103 52-53 0.5839976328109984 1103 54-55 0.6377759719495018 1103 56-57 0.6263727289289989 1103 58-59 0.6221838824527595 1103 60-61 0.5687433763405139 1103 62-63 0.5342166068912846 1103 64-65 0.560596838863141 1103 66-67 0.5382192343020691 1103 68-69 0.5056400489622561 1103 70-71 0.4891417796815887 1103 72-73 0.4582941715065516 1103 74-75 0.4721862690945855 1103 76-77 0.4541692065709171 1103 78-79 0.4509766864793434 1103 80-81 0.477858568210749 1103 82-83 0.4742967849466737 1103 84-85 0.4741600952944367 1103 86-87 0.45517514883186294 1103 88-89 0.4621156589011761 1103 90-91 0.47858734584041684 1103 92-93 0.48448906381838697 1103 94-95 0.49190884345287955 1103 96-97 0.47423600795768905 1103 98-99 0.30153395865317023 1103 100 0.3409746747808384 1104 1 0.39068967937684107 1104 2 0.3856535116562583 1104 3 0.35131028396863684 1104 4 0.3285773505537577 1104 5 0.31619257659423283 1104 6 0.31292571570261885 1104 7 0.3288927161767532 1104 8 0.33306348156526866 1104 9 0.38439252355075837 1104 10-11 0.4121764882814958 1104 12-13 0.36266937370807284 1104 14-15 0.49172341047770374 1104 16-17 0.499862553228823 1104 18-19 0.49537390855775953 1104 20-21 0.4831394496972905 1104 22-23 0.49190423005121175 1104 24-25 0.49995966291286464 1104 26-27 0.48185964757834654 1104 28-29 0.46793402425509356 1104 30-31 0.5238806349972052 1104 32-33 0.48350603683228144 1104 34-35 0.5150512459770056 1104 36-37 0.4974747031970921 1104 38-39 0.5207552525820702 1104 40-41 0.5061705434961894 1104 42-43 0.5328124607637932 1104 44-45 0.5283436536903494 1104 46-47 0.5556640146374292 1104 48-49 0.4952471465981958 1104 50-51 0.39700010902817695 1104 52-53 0.48378856189929564 1104 54-55 0.5474890884023225 1104 56-57 0.5424206062344084 1104 58-59 0.5456510578522895 1104 60-61 0.5352332310827492 1104 62-63 0.5249724125118505 1104 64-65 0.5091206391764231 1104 66-67 0.47189792872981684 1104 68-69 0.47010970266706664 1104 70-71 0.45865645340155936 1104 72-73 0.4359366582209674 1104 74-75 0.4305612391680782 1104 76-77 0.4072197480174964 1104 78-79 0.4288061434122383 1104 80-81 0.45553546398388534 1104 82-83 0.434846782412194 1104 84-85 0.45969445853511814 1104 86-87 0.45070361504993883 1104 88-89 0.4469271437160742 1104 90-91 0.46307002119925045 1104 92-93 0.44675499535782137 1104 94-95 0.44900087864838056 1104 96-97 0.4406351373242323 1104 98-99 0.258843087123644 1104 100 0.35159812035285043 1105 1 0.31662380750287866 1105 2 0.28323710038535665 1105 3 0.28970336459570944 1105 4 0.26960759752894603 1105 5 0.28847451495382614 1105 6 0.25004655816452015 1105 7 0.24842397982319397 1105 8 0.2543935119848726 1105 9 0.2939162136169884 1105 10-11 0.29145955973188364 1105 12-13 0.2870896646249932 1105 14-15 0.3719561918427061 1105 16-17 0.3766309456937833 1105 18-19 0.3795724961407174 1105 20-21 0.3772252045555007 1105 22-23 0.4083173216171261 1105 24-25 0.40717982470593483 1105 26-27 0.39604648510191254 1105 28-29 0.39335620797697146 1105 30-31 0.39970895950175134 1105 32-33 0.3818500736692627 1105 34-35 0.4017526404364702 1105 36-37 0.40696656525964414 1105 38-39 0.42759843213035964 1105 40-41 0.4312940114339696 1105 42-43 0.40550873981953117 1105 44-45 0.43851363503261354 1105 46-47 0.42474180887229096 1105 48-49 0.40055643119466566 1105 50-51 0.3514165646249765 1105 52-53 0.4076742204727779 1105 54-55 0.4818676438007543 1105 56-57 0.46231782734950144 1105 58-59 0.48176683762360284 1105 60-61 0.46219612096813734 1105 62-63 0.45308163656908107 1105 64-65 0.4659096349950076 1105 66-67 0.45898054503796715 1105 68-69 0.43661440949258434 1105 70-71 0.405364653276834 1105 72-73 0.40798878669721717 1105 74-75 0.4252994463671982 1105 76-77 0.4168506690462337 1105 78-79 0.41752262419215214 1105 80-81 0.39368688571822474 1105 82-83 0.408624261052438 1105 84-85 0.4349406893500074 1105 86-87 0.4149477776294219 1105 88-89 0.4275223470615117 1105 90-91 0.4142893914679817 1105 92-93 0.42844599950127105 1105 94-95 0.4314388649231695 1105 96-97 0.41866901743144425 1105 98-99 0.27153235664772524 1105 100 0.2546402353378383 1106 1 0.35041973733358134 1106 2 0.32762822767828226 1106 3 0.3580903817831853 1106 4 0.3297535318814653 1106 5 0.30608790482542503 1106 6 0.28169945885399983 1106 7 0.2771659505550943 1106 8 0.3146404034834944 1106 9 0.3764229618131978 1106 10-11 0.3740246352406089 1106 12-13 0.352407460053378 1106 14-15 0.39330016018209335 1106 16-17 0.4507840261064473 1106 18-19 0.43909158385714164 1106 20-21 0.4677648888881052 1106 22-23 0.4460815234012685 1106 24-25 0.42760763366561605 1106 26-27 0.4143190460950734 1106 28-29 0.4653950746039257 1106 30-31 0.4507749243354624 1106 32-33 0.4610664922665677 1106 34-35 0.4669638591040126 1106 36-37 0.4666387278855808 1106 38-39 0.4618026389989893 1106 40-41 0.46288924767078043 1106 42-43 0.4703758798060065 1106 44-45 0.4383712235679198 1106 46-47 0.4405517639117207 1106 48-49 0.4585869240013807 1106 50-51 0.4569908027419203 1106 52-53 0.43867576830384536 1106 54-55 0.4599118558252755 1106 56-57 0.4606993293253794 1106 58-59 0.4605194802901522 1106 60-61 0.5003114142510228 1106 62-63 0.4764916454366457 1106 64-65 0.428663146341016 1106 66-67 0.4467286355183475 1106 68-69 0.45725234393441383 1106 70-71 0.4137885363124951 1106 72-73 0.4069827178354757 1106 74-75 0.42077383336704344 1106 76-77 0.40957181924812147 1106 78-79 0.37249895881135586 1106 80-81 0.3725693433355559 1106 82-83 0.3570677929953945 1106 84-85 0.42262577222668796 1106 86-87 0.39920746205745417 1106 88-89 0.4299977269448547 1106 90-91 0.4100914358574883 1106 92-93 0.3634691088634767 1106 94-95 0.3576041781889394 1106 96-97 0.3794413669625136 1106 98-99 0.3094006883800091 1106 100 0.37249582744813736 1107 1 0.29267661148234936 1107 2 0.2652336834455369 1107 3 0.2555425768615862 1107 4 0.2823612739381218 1107 5 0.2867987056094279 1107 6 0.2384537155310582 1107 7 0.2333009979105114 1107 8 0.2584671146855797 1107 9 0.3202523459958897 1107 10-11 0.32957305245296453 1107 12-13 0.2939364013944541 1107 14-15 0.34732481584428854 1107 16-17 0.33570317423465923 1107 18-19 0.36821393250540524 1107 20-21 0.36104720749562347 1107 22-23 0.3808901482364959 1107 24-25 0.39432131583361496 1107 26-27 0.37472646759007944 1107 28-29 0.3557407965786652 1107 30-31 0.36724370133813977 1107 32-33 0.37384779451776495 1107 34-35 0.3755308354443869 1107 36-37 0.38528688398336897 1107 38-39 0.3617308984270835 1107 40-41 0.409984579791292 1107 42-43 0.36324674205430796 1107 44-45 0.39650961197089174 1107 46-47 0.36388768424478 1107 48-49 0.35321582341230595 1107 50-51 0.2390647167143598 1107 52-53 0.29773794771323736 1107 54-55 0.35261890891226955 1107 56-57 0.3241821397435203 1107 58-59 0.32480928359976247 1107 60-61 0.3308682535797658 1107 62-63 0.3144073362426454 1107 64-65 0.28072211656988344 1107 66-67 0.28356895504288104 1107 68-69 0.27260348314983673 1107 70-71 0.24087945534203214 1107 72-73 0.2781172760354451 1107 74-75 0.2766422954569592 1107 76-77 0.27953497488348944 1107 78-79 0.27505289376847486 1107 80-81 0.2886082048204486 1107 82-83 0.27962166380096676 1107 84-85 0.3094689340206003 1107 86-87 0.28551149257362596 1107 88-89 0.2873887900658687 1107 90-91 0.286800909417515 1107 92-93 0.3076422673090349 1107 94-95 0.32002603137351926 1107 96-97 0.30034475466249333 1107 98-99 0.223316234310456 1107 100 0.22755366807540867 1108 1 0.11903225328680378 1108 2 0.12470745985917375 1108 3 0.10299229874647864 1108 4 0.13230470133850503 1108 5 0.13351693726579583 1108 6 0.11933059001182045 1108 7 0.09347878026207468 1108 8 0.11603250319627989 1108 9 0.1418756400952006 1108 10-11 0.1572123228647868 1108 12-13 0.14656316452923335 1108 14-15 0.15022809910097124 1108 16-17 0.18152627957850598 1108 18-19 0.17216544495379082 1108 20-21 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-0.7238902475422364 2316 86-87 -0.6291332159559246 2316 88-89 -0.6734543437774221 2316 90-91 -0.6729073843287381 2316 92-93 -0.6511799625127139 2316 94-95 -0.5970865219767312 2316 96-97 -0.6384435938132142 2316 98-99 -0.5298193780207257 2316 100 -0.5758084188147059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 707.0 3 257.0 4 248.0 5 215.0 6 654.0 7 4934.0 8 9488.0 9 12208.0 10 14439.0 11 15314.0 12 16704.0 13 18921.0 14 23117.0 15 31045.0 16 41409.0 17 53109.0 18 58874.0 19 65518.0 20 75322.0 21 90735.0 22 116028.0 23 163029.0 24 228265.0 25 290671.0 26 312503.0 27 284793.0 28 264874.0 29 278269.0 30 321110.0 31 392502.0 32 492173.0 33 647318.0 34 920348.0 35 1501388.0 36 2870571.0 37 5948916.0 38 7097507.0 39 1917492.0 40 3716.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.32946869096332 30.59056250553429 27.966667092981872 24.113301710520517 2 22.7249382121173 31.652724628916605 25.304812760230455 20.31752439873563 3 25.41514183111592 24.806678998386207 26.42878818459239 23.349390985905476 4 21.212914376827605 27.141354773303135 24.063281887473945 27.58244896239532 5 23.376033848968216 29.53953697195304 25.702702624587115 21.38172655449163 6 27.62104448125422 26.707059267494493 24.98285142967173 20.689044821579557 7 22.77515171031797 25.38844299799456 29.37996153691667 22.456443754770802 8 26.640919978946222 26.35132061039688 24.830805911043623 22.176953499613276 9 25.76560017743919 25.233331435841844 26.37705901053401 22.624009376184958 10-11 22.311090607542777 28.264617165128026 27.224347677285 22.199944550044197 12-13 22.1756524792752 26.33979245865922 27.313661067557483 24.170893994508102 14-15 22.18298227188878 30.029972948741 25.533074640528326 22.253970138841893 16-17 24.209708118852348 26.034711568251968 25.621570827397615 24.13400948549807 18-19 22.21822967645998 27.96248144288348 25.67644850775082 24.14284037290572 20-21 24.13249630724082 28.0677343504791 25.64507968001017 22.15468966226991 22-23 24.08845255146873 26.17315807609063 27.50244515257465 22.23594421986599 24-25 22.341843024962362 27.78384855741799 25.55264040604701 24.321668011572633 26-27 22.40424528104233 25.981820200392995 29.372491105761046 22.241443412803626 28-29 22.47860013391215 26.01643210614161 29.29473504263212 22.21023271731412 30-31 22.37587666085896 25.950992119167598 29.336065271155782 22.33706594881766 32-33 22.468385365868542 26.114854316698583 29.139459127415556 22.277301190017322 34-35 22.46366713850559 26.22993880304839 29.05282619568229 22.253567862763724 36-37 22.403693635053664 26.20120449644285 29.121894195520504 22.273207672982988 38-39 22.46989648588363 26.199795159268234 29.147445600355613 22.18286275449252 40-41 22.488465228771478 26.26964872920443 29.084668948683706 22.157217093340385 42-43 22.51840421431262 26.273265507212084 29.02192776419626 22.186402514279038 44-45 22.57654331616178 26.331371900600487 28.987229639143465 22.10485514409427 46-47 22.633840561389416 26.412300480402074 28.886759310968817 22.06709964723969 48-49 22.693943975633708 26.422513493459622 28.804856577479892 22.078685953426778 50-51 22.790308288996336 26.458940550957706 28.733886182353434 22.016864977692528 52-53 22.92397292047717 26.570520073828835 28.518210715677316 21.98729629001668 54-55 23.18227161587522 26.73651443035 28.035669164429304 22.045544789345477 56-57 23.520326149010863 27.081167435210833 27.37125588258642 22.027250533191882 58-59 23.704457394953764 27.343353777319624 27.002932142555707 21.949256685170905 60-61 23.83757813197532 27.44215549098919 26.85459127850411 21.86567509853138 62-63 23.959351835160202 27.532518599007638 26.759327772061177 21.74880179377098 64-65 24.101548221619954 27.581803472129728 26.704608972771986 21.612039333478332 66-67 24.212111299008598 27.471112753092264 26.806073768844534 21.5107021790546 68-69 24.334950921864344 27.400712051421074 26.90928378967004 21.355053237044544 70-71 24.485765551331934 27.316232709120154 26.980436606548142 21.217565132999773 72-73 24.62560235131536 27.272693794289328 26.964506646543647 21.137197207851667 74-75 24.864082845145056 27.3141206799104 26.832560852770847 20.989235622173695 76-77 25.09085562778936 27.37881597756875 26.61268802908829 20.9176403655536 78-79 25.311086625326933 27.42104796072838 26.397838499972593 20.870026913972094 80-81 25.52665750611721 27.517834985968147 26.158209311888882 20.797298196025764 82-83 25.68059013347705 27.548094930305357 25.99660198806688 20.774712948150718 84-85 25.812694898394955 27.505099122879656 25.92433339077582 20.75787258794957 86-87 25.977134624959575 27.486241890442624 25.82832885539983 20.708294629197972 88-89 26.08401439779921 27.500718549197746 25.761277112630776 20.653989940372266 90-91 26.181242877877192 27.446563631187608 25.741258252954363 20.630935237980836 92-93 26.265706466095356 27.489930660281154 25.712168706134985 20.53219416748851 94-95 26.32740412857283 27.538743033329872 25.673138422613196 20.4607144154841 96-97 26.365566293867282 27.596498832685175 25.613379925830742 20.424554947616805 98-99 26.4134603428228 27.712440875517185 25.53102300592548 20.343075775734533 100 26.390820581311804 27.83500774437682 25.423052522036908 20.351119152274467 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45782.0 1 30326.0 2 11824.5 3 8020.5 4 7037.5 5 6792.0 6 6906.0 7 7128.5 8 7093.5 9 7247.5 10 7881.5 11 11479.0 12 92086.5 13 105425.0 14 39647.5 15 39837.5 16 44310.5 17 48956.5 18 53231.0 19 57694.5 20 63174.0 21 71896.5 22 85312.5 23 100809.0 24 115777.0 25 132501.0 26 150856.5 27 173678.0 28 206817.0 29 241630.5 30 269302.5 31 301953.0 32 337174.0 33 362069.5 34 388446.5 35 429939.0 36 472362.0 37 501558.0 38 536068.5 39 575973.0 40 606604.5 41 645557.0 42 695324.0 43 722341.0 44 737661.0 45 768647.5 46 809521.5 47 850933.0 48 887799.0 49 918785.0 50 940315.0 51 955200.0 52 960021.0 53 952227.0 54 932962.5 55 897463.5 56 849262.0 57 788464.0 58 708342.0 59 610691.5 60 507285.0 61 409514.5 62 323085.0 63 247748.0 64 185154.5 65 136098.5 66 98291.5 67 71809.5 68 53302.5 69 40541.5 70 31485.0 71 24767.0 72 19976.5 73 15690.5 74 12859.5 75 11638.0 76 8854.5 77 4913.0 78 2768.5 79 1856.0 80 1305.0 81 1009.5 82 733.0 83 514.5 84 417.5 85 368.0 86 294.0 87 228.0 88 176.0 89 153.5 90 132.5 91 106.5 92 86.0 93 67.0 94 55.0 95 50.0 96 43.0 97 32.0 98 19.5 99 13.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13511253812382673 2 0.05174358302896709 3 0.019190804553939685 4 0.07621002842785374 5 0.07960238345074176 6 0.08119280409096864 7 0.04896543137353242 8 0.046504550331749134 9 0.03410252339555539 10-11 0.023475991624218505 12-13 0.015281867890875668 14-15 0.012306438994901338 16-17 0.01759835012772786 18-19 0.016927200752696058 20-21 0.02378512709393012 22-23 0.02007956902936059 24-25 0.017433613462947327 26-27 0.01583099010681078 28-29 0.017885113951605087 30-31 0.015654050726120576 32-33 0.012674554258176358 34-35 0.010179098854649017 36-37 0.008013116780682743 38-39 0.016801106021629476 40-41 0.025578926332651485 42-43 0.025841284724709375 44-45 0.024645418565561794 46-47 0.02655717739140996 48-49 0.022996018131771517 50-51 0.023217700804130506 52-53 0.01978263627555864 54-55 0.024789817370492885 56-57 0.03133657445603038 58-59 0.024025113840153614 60-61 0.02377699194999034 62-63 0.031078283635942384 64-65 0.03179010873067308 66-67 0.024330181737895345 68-69 0.024930148603454074 70-71 0.03565430210206832 72-73 0.040067617689398656 74-75 0.0409624835227744 76-77 0.04230884984480789 78-79 0.036526796289609656 80-81 0.03224160921933084 82-83 0.03480214577437642 84-85 0.03817823050938488 86-87 0.038100946641956984 88-89 0.037134898299108175 90-91 0.040085921763263165 92-93 0.033470015954237535 94-95 0.0333866307288548 96-97 0.03336222529703546 98-99 0.31136653293710304 100 0.1486982285032584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4584691E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.540815721855616 #Duplication Level Percentage of deduplicated Percentage of total 1 77.53672712814253 38.412327103311114 2 10.906685525881338 10.806521955478345 3 4.205483824675047 6.2502929753841325 4 2.1649881419147925 4.290211143144132 5 1.2788627048979193 3.1677950798450816 6 0.8047367673951666 2.392038953887544 7 0.5664316344659901 1.9643039655476362 8 0.3914923917787794 1.551588195009681 9 0.2873208853438158 1.2810699930472544 >10 1.6485031903556955 15.090525565335799 >50 0.13917391085473094 4.656329042777911 >100 0.06864084662053772 6.075377545979817 >500 5.503522252978585E-4 0.18300944475788103 >1k 3.6176244848764124E-4 0.29625047074287136 >5k 8.186599970216841E-6 0.03330142171452746 >10k+ 3.274639988086736E-5 3.54905714403648 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 791271 3.2185517401866064 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42677 0.17359176895898346 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003550990329713723 2.0337859849448585E-5 0.0 0.0 1.2202715909669152E-5 2 0.003579463333502951 3.2540575759117734E-5 0.0 0.0 2.0337859849448585E-5 3 0.0035875984774427303 4.067571969889717E-5 0.0 8.135143939779433E-6 2.847300378922802E-5 4 0.0036038687653222896 4.474329166878689E-5 0.0 8.135143939779433E-6 3.2540575759117734E-5 5 0.003607936337292179 6.914872348812519E-5 0.0 8.135143939779433E-6 3.2540575759117734E-5 6 0.003640476913051297 6.914872348812519E-5 0.0 8.135143939779433E-6 3.2540575759117734E-5 7 0.0036486120569910762 7.32162954580149E-5 0.0 8.135143939779433E-6 3.660814772900745E-5 8 0.0036486120569910762 8.948658333757378E-5 0.0 8.135143939779433E-6 4.474329166878689E-5 9 0.0036608147729007452 1.0168929924724293E-4 0.0 1.2202715909669152E-5 4.474329166878689E-5 10-11 0.0036709837028254698 1.1389201515691209E-4 0.0 1.2202715909669152E-5 5.084464962362147E-5 12-13 0.003691321562674918 1.321960890214158E-4 0.0 1.6270287879558867E-5 5.89797935634009E-5 14-15 0.003725895924418981 1.525339488708644E-4 0.0 2.6439217804283163E-5 6.914872348812519E-5 16-17 0.003756402714193154 1.6880423675042324E-4 0.0 2.847300378922802E-5 7.32162954580149E-5 18-19 0.0037767405740426023 1.9320966856976158E-4 0.0 2.847300378922802E-5 7.931765341284949E-5 20-21 0.003825551437681279 2.1761510038909986E-4 0.0 2.847300378922802E-5 8.948658333757378E-5 22-23 0.00415705855322729 2.359191742536036E-4 0.0 2.847300378922802E-5 9.762172727735321E-5 24-25 0.005751546765424061 2.4812189016327276E-4 0.0 2.847300378922802E-5 1.1185822917196721E-4 26-27 0.011598681472140528 2.7252732198261104E-4 0.0 2.847300378922802E-5 1.1999337311174665E-4 28-29 0.028340807700206604 2.887976098621699E-4 0.0 3.2540575759117734E-5 1.3016230303647094E-4 30-31 0.06619159866601537 3.132030416815082E-4 0.0 4.067571969889717E-5 1.4439880493108496E-4 32-33 0.1365260193833634 3.1523682766645305E-4 0.0 4.270950568384203E-5 1.7083802273536812E-4 34-35 0.24676128733934463 3.2947332956106707E-4 0.0 4.474329166878689E-5 1.7490559470525782E-4 36-37 0.4038448154585307 3.437098314556811E-4 0.0 4.677707765373175E-5 1.911758825848167E-4 38-39 0.6081365838602568 3.518449753954605E-4 0.0 5.2878435608566326E-5 2.115137424342653E-4 40-41 0.8611985401809605 3.6811526327501943E-4 0.0 5.2878435608566326E-5 2.115137424342653E-4 42-43 1.1622415754584834 3.8438555115457825E-4 0.0 5.2878435608566326E-5 2.196488863740447E-4 44-45 1.5088068424370271 4.006558390341372E-4 0.0 5.694600757845604E-5 2.2778403031382418E-4 46-47 1.9044270273724409 4.3116262880831E-4 0.0 5.694600757845604E-5 2.399867462234933E-4 48-49 2.3455145317872814 4.6166941858248286E-4 0.0 6.914872348812519E-5 2.58290820087997E-4 50-51 2.833106586533872 4.942099943416006E-4 0.0 7.32162954580149E-5 2.6439217804283163E-4 52-53 3.3637477892237895 5.043789242663249E-4 0.0 7.32162954580149E-5 2.765948939525008E-4 54-55 3.932146228724209 5.409870719953324E-4 0.0 7.728386742790462E-5 2.908313958471148E-4 56-57 4.536168463536923 5.674262897996155E-4 0.0 8.33852253827392E-5 3.010003257718391E-4 58-59 5.1753589256013015 5.857303636641192E-4 0.0 8.541901136768406E-5 3.1116925569656334E-4 60-61 5.845892063479667 6.020006515436782E-4 0.0 8.948658333757378E-5 3.2540575759117737E-4 62-63 6.547609241865191 6.121695814684024E-4 0.0 9.152036932251863E-5 3.437098314556811E-4 64-65 7.276328996772829 6.223385113931267E-4 0.0 9.35541553074635E-5 3.477774034255708E-4 66-67 8.027322775787582 6.304736553329061E-4 0.0 9.558794129240836E-5 3.5998011933523997E-4 68-69 8.80049905854013 6.426763712425753E-4 0.0 1.0168929924724293E-4 3.7218283524490913E-4 70-71 9.592664801034108 6.589466591221341E-4 0.0 1.0168929924724293E-4 3.823517651696334E-4 72-73 10.401651987409563 6.731831610167483E-4 0.0 1.0575687121713265E-4 3.8438555115457825E-4 74-75 11.229978444715861 6.853858769264173E-4 0.0 1.1389201515691208E-4 3.9455448107930256E-4 76-77 12.075309386642282 6.975885928360865E-4 0.0 1.179595871268018E-4 3.9862205304919227E-4 78-79 12.93527748630235 7.219940246554247E-4 0.0 1.2406094508163638E-4 4.108247689588614E-4 80-81 13.810559994429054 7.463994564747632E-4 0.0 1.2609473106658123E-4 4.230274848685306E-4 82-83 14.696660210209679 7.708048882941014E-4 0.0 1.2812851705152608E-4 4.2912884282336516E-4 84-85 15.589819697144048 7.830076042037706E-4 0.0 1.3016230303647094E-4 4.4133155873303433E-4 86-87 16.4919786870618 8.175819659478332E-4 2.0337859849448584E-6 1.3016230303647094E-4 4.555680606276483E-4 88 17.174053560404726 8.33852253827392E-4 4.067571969889717E-6 1.3016230303647094E-4 4.5963563259753806E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 111495 0.0 79.99635 8 GTGGTAT 111560 0.0 79.93478 6 AGTGGTA 112500 0.0 79.34539 5 TGGTATC 112965 0.0 78.79713 7 GTATCAA 113060 0.0 78.683105 9 AAGCAGT 117265 0.0 76.97778 1 GCAGTGG 121235 0.0 74.43662 3 CAGTGGT 121600 0.0 73.67956 4 AGCAGTG 128000 0.0 70.739365 2 CAACGCA 110155 0.0 42.44203 12-13 ATCAACG 111845 0.0 41.832092 10-11 ACGCAGA 113945 0.0 41.00731 14-15 AGAGTAC 113190 0.0 40.66804 18-19 TCAACGC 109995 0.0 40.425674 12-13 GAGTACT 104125 0.0 40.061356 20-21 AGTACTT 110180 0.0 39.868427 20-21 AACGCAG 112005 0.0 39.620064 14-15 TATCAAC 115100 0.0 38.61 10-11 GTACTTT 108875 0.0 38.195667 22-23 CGCAGAG 116055 0.0 38.11675 16-17 >>END_MODULE