FastQCFastQC Report
Thu 26 May 2016
SRR1660655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24584691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7943603.231116470001596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA427560.17391310714460476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1122950.078.87828
GTGGTAT1129650.078.589186
AGTGGTA1135950.078.293935
TGGTATC1136450.077.970127
AAGCAGT1160800.077.3914641
GTATCAA1143250.077.3419959
GCAGTGG1214050.073.756443
CAGTGGT1219550.073.219664
AGCAGTG1278400.070.3855062
CAACGCA1110450.042.0322312-13
ATCAACG1124850.041.49197410-11
AGAGTAC1139900.040.5639418-19
ACGCAGA1151100.040.52873214-15
TCAACGC1105250.039.9305112-13
AGTACTT1107700.039.65319420-21
GAGTACT1048000.039.5874820-21
AACGCAG1127550.039.05657214-15
TATCAAC1159100.038.17259610-11
GTACTTT1095250.037.713622-23
CGCAGAG1168250.037.61855716-17
Produced by FastQC (version 0.11.5)