##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660655_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24584691 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77203549151787 34.0 31.0 34.0 31.0 34.0 2 32.95540139186618 34.0 31.0 34.0 31.0 34.0 3 33.01010901458961 34.0 31.0 34.0 31.0 34.0 4 36.39066067578397 37.0 37.0 37.0 35.0 37.0 5 36.31521954048558 37.0 37.0 37.0 35.0 37.0 6 36.30819773166968 37.0 37.0 37.0 35.0 37.0 7 36.25855972727093 37.0 37.0 37.0 35.0 37.0 8 36.276466928138326 37.0 37.0 37.0 35.0 37.0 9 38.054368793978334 39.0 38.0 39.0 35.0 39.0 10-11 37.99784384111234 39.0 38.0 39.0 35.0 39.0 12-13 38.03721559892699 39.0 38.0 39.0 35.0 39.0 14-15 39.54536294558268 41.0 40.0 41.0 37.0 41.0 16-17 39.51751476152374 41.0 39.0 41.0 37.0 41.0 18-19 39.481050870234654 41.0 39.0 41.0 37.0 41.0 20-21 39.44185119105219 41.0 39.0 41.0 36.5 41.0 22-23 39.32176825000566 41.0 39.0 41.0 36.0 41.0 24-25 39.31283710256923 41.0 39.0 41.0 36.0 41.0 26-27 39.25595042052796 41.0 39.0 41.0 36.0 41.0 28-29 39.17940258024801 40.5 39.0 41.0 36.0 41.0 30-31 39.08995848269966 40.0 39.0 41.0 35.5 41.0 32-33 38.99240976833917 40.0 39.0 41.0 35.0 41.0 34-35 38.89015475118235 40.0 38.0 41.0 35.0 41.0 36-37 38.75013301977235 40.0 38.0 41.0 35.0 41.0 38-39 38.4728497096018 40.0 38.0 41.0 35.0 41.0 40-41 38.34990600451313 40.0 38.0 41.0 34.0 41.0 42-43 38.2177908194982 40.0 38.0 41.0 34.0 41.0 44-45 38.08471406046958 40.0 37.0 41.0 34.0 41.0 46-47 37.940447675343975 40.0 37.0 41.0 33.0 41.0 48-49 37.77581676743466 40.0 37.0 41.0 33.0 41.0 50-51 37.813918405563854 40.0 37.0 41.0 33.0 41.0 52-53 37.72450290304646 40.0 36.5 41.0 33.0 41.0 54-55 37.30963954356798 40.0 36.0 41.0 32.5 41.0 56-57 37.0462143290717 39.5 36.0 41.0 32.0 41.0 58-59 36.82518932208666 39.0 35.0 41.0 31.5 41.0 60-61 36.59388853006125 39.0 35.0 41.0 31.0 41.0 62-63 36.32943761627917 38.5 35.0 40.5 31.0 41.0 64-65 36.049991984849434 37.5 35.0 40.0 31.0 41.0 66-67 35.761627713767076 37.0 35.0 40.0 31.0 41.0 68-69 35.45260696992287 36.5 35.0 39.0 30.0 41.0 70-71 35.125535012825665 36.0 35.0 39.0 30.0 41.0 72-73 34.773215555159915 35.5 34.5 38.5 30.0 40.5 74-75 34.42834066940276 35.0 34.0 37.0 29.5 39.5 76-77 32.588461819593334 34.5 32.0 35.5 27.0 38.5 78-79 33.742663208579685 35.0 34.0 36.0 29.0 39.0 80-81 33.7243672291834 35.0 34.0 36.0 29.0 38.5 82-83 33.51284106031676 35.0 34.0 36.0 29.0 37.0 84-85 33.298196202669374 35.0 34.0 35.0 29.0 37.0 86-87 33.119989061485455 35.0 34.0 35.0 29.0 36.5 88-89 32.962912326211466 35.0 34.0 35.0 29.0 36.0 90-91 32.85662164718686 35.0 34.0 35.0 29.0 36.0 92-93 32.766754542491505 35.0 34.0 35.0 29.0 36.0 94-95 32.67049539081049 35.0 34.0 35.0 29.0 35.0 96-97 32.59823471037322 35.0 34.0 35.0 28.0 35.0 98-99 32.50281837994221 35.0 34.0 35.0 27.0 35.0 100 32.45113802731952 35.0 34.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39381210158141045 1101 2 0.3323579224619664 1101 3 0.3177527043508732 1101 4 0.2227942910026215 1101 5 0.24239771437606095 1101 6 0.24996815931211813 1101 7 0.2454699743279818 1101 8 0.256818735141529 1101 9 0.32541244441708983 1101 10-11 0.3239593829404015 1101 12-13 0.33268812606204534 1101 14-15 0.40734692467510314 1101 16-17 0.4444769494635068 1101 18-19 0.4596019477107589 1101 20-21 0.4513839787561338 1101 22-23 0.44947829209840506 1101 24-25 0.4376833463144507 1101 26-27 0.4794007284272084 1101 28-29 0.4905036055245162 1101 30-31 0.5150395163538164 1101 32-33 0.5295517261071865 1101 34-35 0.5168535608901692 1101 36-37 0.5594440297857446 1101 38-39 0.5409946069940901 1101 40-41 0.5297116116625347 1101 42-43 0.5368899391065654 1101 44-45 0.5302565130615875 1101 46-47 0.5090824252315684 1101 48-49 0.5285329759691919 1101 50-51 0.48071987919554715 1101 52-53 0.5237569685818997 1101 54-55 0.37216640310705174 1101 56-57 0.3611538305312436 1101 58-59 0.3898264975057728 1101 60-61 0.35989561380197443 1101 62-63 0.30758955555613454 1101 64-65 0.3070190372266808 1101 66-67 0.3588977291162152 1101 68-69 0.33449086357902047 1101 70-71 0.33200782690332886 1101 72-73 0.3643217793627045 1101 74-75 0.3840044165518677 1101 76-77 0.41020641856962925 1101 78-79 0.4814665905173072 1101 80-81 0.4284370606981085 1101 82-83 0.4494558952932479 1101 84-85 0.4031435413132485 1101 86-87 0.40092434382771813 1101 88-89 0.40239092967117784 1101 90-91 0.39223483091701183 1101 92-93 0.35305590600530223 1101 94-95 0.3833449876330235 1101 96-97 0.3657618578103907 1101 98-99 0.3557597264955703 1101 100 0.3559583221018343 1102 1 0.3049795512507245 1102 2 0.2539880250067412 1102 3 0.27489023144801905 1102 4 0.19442728459186043 1102 5 0.2097112105443628 1102 6 0.21256597974159774 1102 7 0.22019860232862243 1102 8 0.21930944010709652 1102 9 0.24744127901490032 1102 10-11 0.2931159937758494 1102 12-13 0.28425995378501057 1102 14-15 0.36234950007956 1102 16-17 0.3699219787798498 1102 18-19 0.3795332465789656 1102 20-21 0.36953917147000226 1102 22-23 0.38772324918480194 1102 24-25 0.3975993829967095 1102 26-27 0.4031685360403472 1102 28-29 0.4146868151751235 1102 30-31 0.43011261277988666 1102 32-33 0.40495836163775323 1102 34-35 0.3973378661954783 1102 36-37 0.41033647578645827 1102 38-39 0.41786213291526764 1102 40-41 0.42884211392509286 1102 42-43 0.40458022683341 1102 44-45 0.4281331729613598 1102 46-47 0.42100291067016826 1102 48-49 0.41819678622071166 1102 50-51 0.39154382653322983 1102 52-53 0.4142587866042504 1102 54-55 0.32745500516573145 1102 56-57 0.32081026797472845 1102 58-59 0.30468696428747677 1102 60-61 0.28509869127075405 1102 62-63 0.2946372355131288 1102 64-65 0.30230871019044514 1102 66-67 0.26856295448637724 1102 68-69 0.2766904916503705 1102 70-71 0.3241960134925961 1102 72-73 0.33583966770756746 1102 74-75 0.38390831459066277 1102 76-77 0.386712803918158 1102 78-79 0.3946728316896113 1102 80-81 0.36274666618021456 1102 82-83 0.34284244830057276 1102 84-85 0.37283753039219647 1102 86-87 0.38337640687097263 1102 88-89 0.39241403548408726 1102 90-91 0.3529716688517155 1102 92-93 0.3200311071325288 1102 94-95 0.31933520454819586 1102 96-97 0.35245729284412164 1102 98-99 0.362480417054833 1102 100 0.3930292813926428 1103 1 0.2602303616385697 1103 2 0.23951063781193938 1103 3 0.24158799412833787 1103 4 0.15939322523794885 1103 5 0.15653803424401502 1103 6 0.17260186739091665 1103 7 0.19354684885435347 1103 8 0.1782029097521871 1103 9 0.25019701479374845 1103 10-11 0.260331340604516 1103 12-13 0.25792520015402687 1103 14-15 0.3265256016753284 1103 16-17 0.30787815075559166 1103 18-19 0.3343380826893423 1103 20-21 0.346461339215864 1103 22-23 0.36162550913352476 1103 24-25 0.3488224224837779 1103 26-27 0.32369074138673426 1103 28-29 0.35253103370856564 1103 30-31 0.37623593082496853 1103 32-33 0.34013589527660315 1103 34-35 0.36179138819862544 1103 36-37 0.35419407512709 1103 38-39 0.37478643801392053 1103 40-41 0.3683188598912821 1103 42-43 0.3334179381938256 1103 44-45 0.31539282669447033 1103 46-47 0.3336961682251456 1103 48-49 0.33603061863447437 1103 50-51 0.3774688123475798 1103 52-53 0.32778640801911507 1103 54-55 0.21937122855726443 1103 56-57 0.20934513102840668 1103 58-59 0.2517505270347016 1103 60-61 0.18797509745625263 1103 62-63 0.16679604718181196 1103 64-65 0.15120659438805006 1103 66-67 0.15795022840818973 1103 68-69 0.13754208028846904 1103 70-71 0.12642741745234787 1103 72-73 0.15615986067577126 1103 74-75 0.1628817843650907 1103 76-77 0.07318533026989371 1103 78-79 0.28301462401564237 1103 80-81 0.3021977617368492 1103 82-83 0.28165883064013286 1103 84-85 0.2908719496412786 1103 86-87 0.299884284534933 1103 88-89 0.3326827952504985 1103 90-91 0.28889947981671327 1103 92-93 0.2694130709198461 1103 94-95 0.2756928487691397 1103 96-97 0.29996904636783484 1103 98-99 0.2828404877754238 1103 100 0.28865538266340707 1104 1 0.21909105749636382 1104 2 0.20838575653984748 1104 3 0.19561797815143933 1104 4 0.15782537380883355 1104 5 0.1517429130582144 1104 6 0.1454759529963212 1104 7 0.16540864252355192 1104 8 0.15833719492815135 1104 9 0.19284777127914055 1104 10-11 0.20327757763359244 1104 12-13 0.18198204669028684 1104 14-15 0.22594551144813835 1104 16-17 0.25375028633833807 1104 18-19 0.24949202189450403 1104 20-21 0.2632913335382838 1104 22-23 0.29055867227422283 1104 24-25 0.26649121095748285 1104 26-27 0.26743369758185764 1104 28-29 0.2684466997189787 1104 30-31 0.2471452800427656 1104 32-33 0.2625771495295197 1104 34-35 0.2885850673325834 1104 36-37 0.2878578434771697 1104 38-39 0.29273869746403136 1104 40-41 0.3034991387127164 1104 42-43 0.2673797520028529 1104 44-45 0.3154408488247853 1104 46-47 0.2950551468808271 1104 48-49 0.29574862214427355 1104 50-51 0.2741824183545418 1104 52-53 0.28556624973840883 1104 54-55 0.2225335350624178 1104 56-57 0.22647944280570442 1104 58-59 0.22856226709151883 1104 60-61 0.1767001415051297 1104 62-63 0.17141153553594535 1104 64-65 0.2096487327332568 1104 66-67 0.17208369768979281 1104 68-69 0.14489087837240078 1104 70-71 0.1799559366794341 1104 72-73 0.19176402004472237 1104 74-75 0.19130536374429852 1104 76-77 0.1926311561309504 1104 78-79 0.3157423767441472 1104 80-81 0.28506988242119036 1104 82-83 0.2686412401354161 1104 84-85 0.2465354849656407 1104 86-87 0.30369400447141004 1104 88-89 0.3186067028351047 1104 90-91 0.29766035770303034 1104 92-93 0.2815030993025829 1104 94-95 0.31515170804247816 1104 96-97 0.3043013692298402 1104 98-99 0.27140138185863805 1104 100 0.2623468981169239 1105 1 0.17735852934212204 1105 2 0.1423172740660945 1105 3 0.1168000077764404 1105 4 0.10940015136045389 1105 5 0.08767145013177924 1105 6 0.10531897454801964 1105 7 0.11985182742757416 1105 8 0.11011558854502113 1105 9 0.13442076483298848 1105 10-11 0.14843752514860142 1105 12-13 0.12743578348943885 1105 14-15 0.17678707103892322 1105 16-17 0.1719580308609565 1105 18-19 0.20383229943822556 1105 20-21 0.1887884573883909 1105 22-23 0.17866338318709296 1105 24-25 0.18773278067342147 1105 26-27 0.1601824599898336 1105 28-29 0.1862703452413328 1105 30-31 0.18090539169968167 1105 32-33 0.21286627963827698 1105 34-35 0.1978763603680065 1105 36-37 0.1572556260641491 1105 38-39 0.17671693757150564 1105 40-41 0.18286775297601565 1105 42-43 0.19047343895859115 1105 44-45 0.23143901191648553 1105 46-47 0.20004427932850888 1105 48-49 0.22782100017332851 1105 50-51 0.21655003157236763 1105 52-53 0.20009855439671043 1105 54-55 0.10362363031829602 1105 56-57 0.11612990922954225 1105 58-59 0.11486875197025626 1105 60-61 0.0969721787589819 1105 62-63 0.039273479660586474 1105 64-65 0.10473003852521146 1105 66-67 0.11568844828556735 1105 68-69 0.07452762716079775 1105 70-71 0.08650733555279544 1105 72-73 0.1276110304738225 1105 74-75 0.1507634904118973 1105 76-77 0.11338381167507805 1105 78-79 0.24666349515135977 1105 80-81 0.277373475709048 1105 82-83 0.2764145063368062 1105 84-85 0.26227384112313956 1105 86-87 0.26999851396225694 1105 88-89 0.2730588157078202 1105 90-91 0.2734403829783716 1105 92-93 0.24468123532944475 1105 94-95 0.2657312717035083 1105 96-97 0.24903260835046126 1105 98-99 0.2554652517172542 1105 100 0.2831868728278977 1106 1 0.20121602193798793 1106 2 0.17552916015621634 1106 3 0.16732341366446946 1106 4 0.11959130255029748 1106 5 0.1106567584931355 1106 6 0.1271253850440175 1106 7 0.14570053140313632 1106 8 0.14430504596578686 1106 9 0.1882753755885389 1106 10-11 0.18307113240653194 1106 12-13 0.15163565135807033 1106 14-15 0.2166567812671616 1106 16-17 0.2234234824926844 1106 18-19 0.22702174795119845 1106 20-21 0.197294500214646 1106 22-23 0.21429335913118308 1106 24-25 0.2543831486069976 1106 26-27 0.2124825512132844 1106 28-29 0.22980841545253128 1106 30-31 0.2061251810035074 1106 32-33 0.2594010064148904 1106 34-35 0.25487392923755436 1106 36-37 0.24854065329805053 1106 38-39 0.25937572774784456 1106 40-41 0.26036903315538495 1106 42-43 0.2659468686973909 1106 44-45 0.28921836465216444 1106 46-47 0.2706130381959184 1106 48-49 0.2361256108862122 1106 50-51 0.21949446044196463 1106 52-53 0.21347332672340968 1106 54-55 0.15163292419235574 1106 56-57 0.1899498399852675 1106 58-59 0.16430633030417852 1106 60-61 0.12771549642209123 1106 62-63 0.11960401109732999 1106 64-65 0.12876737620770484 1106 66-67 0.10871634694837695 1106 68-69 0.1342948530312711 1106 70-71 0.09618297008402976 1106 72-73 0.11880425172815734 1106 74-75 0.1479411757814333 1106 76-77 0.08099124538635039 1106 78-79 0.168942398021791 1106 80-81 0.20255108971175417 1106 82-83 0.2558700133641949 1106 84-85 0.28075957849521416 1106 86-87 0.29839784166520644 1106 88-89 0.3114529825081078 1106 90-91 0.2658166719109687 1106 92-93 0.24223611842656823 1106 94-95 0.2524754985012123 1106 96-97 0.2331280189117848 1106 98-99 0.28275512074938547 1106 100 0.31433682942701324 1107 1 0.1429707633155317 1107 2 0.13604184139989428 1107 3 0.12469110602507527 1107 4 0.07387675424749318 1107 5 0.08287922870855624 1107 6 0.09030509950910215 1107 7 0.08638343874354604 1107 8 0.11518865138181411 1107 9 0.13575777089278773 1107 10-11 0.1509779796562043 1107 12-13 0.12871102477790686 1107 14-15 0.16249489032583853 1107 16-17 0.11241533979187324 1107 18-19 0.1557048456969241 1107 20-21 0.15404047287932343 1107 22-23 0.15972881039179043 1107 24-25 0.1505606259655181 1107 26-27 0.13496404438658516 1107 28-29 0.17003541253880172 1107 30-31 0.1476868418546644 1107 32-33 0.15541633631504936 1107 34-35 0.1424147144666037 1107 36-37 0.1380957298851797 1107 38-39 0.12554954579591282 1107 40-41 0.16345867129260938 1107 42-43 0.10065163041631564 1107 44-45 0.06788311461353658 1107 46-47 0.11859008415318328 1107 48-49 0.11541376674878023 1107 50-51 0.11151721044718244 1107 52-53 0.12412918617309998 1107 54-55 0.08640401146998045 1107 56-57 0.08715828373497203 1107 58-59 0.05286390176230782 1107 60-61 -0.026095915530014224 1107 62-63 0.006784041954219333 1107 64-65 0.03448833431799869 1107 66-67 0.03717958090206963 1107 68-69 -0.08153202327191877 1107 70-71 -0.05483393197421549 1107 72-73 -0.022612952341255266 1107 74-75 0.015689889498609944 1107 76-77 -0.07425606278853536 1107 78-79 0.06859124633246694 1107 80-81 0.11378991954855877 1107 82-83 0.12967989102543953 1107 84-85 0.14826459434332406 1107 86-87 0.15632953738806776 1107 88-89 0.1427490747173863 1107 90-91 0.1494012208713329 1107 92-93 0.11551209939708684 1107 94-95 0.17038176883256995 1107 96-97 0.13444542858687214 1107 98-99 0.1260473093377854 1107 100 0.15506080711439552 1108 1 0.03218611709177566 1108 2 0.03355927997172614 1108 3 -8.160547035629406E-5 1108 4 0.014969931017063232 1108 5 0.028105826265253597 1108 6 0.03402588615443136 1108 7 0.004658809358097926 1108 8 0.028876417297567514 1108 9 -0.002247349640022378 1108 10-11 0.01598463095574232 1108 12-13 0.001837712465082575 1108 14-15 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-0.514240870908587 2316 54-55 -0.4701039321307192 2316 56-57 -0.5018832897569041 2316 58-59 -0.49155019287988466 2316 60-61 -0.4181148615284016 2316 62-63 -0.3864924709105466 2316 64-65 -0.3887542874015111 2316 66-67 -0.41240817801926966 2316 68-69 -0.3862073519687428 2316 70-71 -0.40245281720626735 2316 72-73 -0.45534244754323794 2316 74-75 -0.45248761174718055 2316 76-77 -0.5089042345006831 2316 78-79 -0.7061256000987086 2316 80-81 -0.6721884984082109 2316 82-83 -0.664260965224095 2316 84-85 -0.6274792217255296 2316 86-87 -0.6359478419093065 2316 88-89 -0.716900416739044 2316 90-91 -0.6285694367230548 2316 92-93 -0.5655370299055633 2316 94-95 -0.5483645173524181 2316 96-97 -0.5930352647546329 2316 98-99 -1.3853319353619504 2316 100 -0.9420220505545416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 13.0 7 266.0 8 482.0 9 163.0 10 70.0 11 65.0 12 91.0 13 387.0 14 1568.0 15 4786.0 16 10292.0 17 16577.0 18 22396.0 19 26569.0 20 33034.0 21 47092.0 22 70544.0 23 113406.0 24 188809.0 25 263773.0 26 285982.0 27 258022.0 28 241001.0 29 261638.0 30 307431.0 31 373761.0 32 463457.0 33 602315.0 34 857077.0 35 1445537.0 36 2904422.0 37 6399319.0 38 7656014.0 39 1726446.0 40 1886.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.69929806394974 30.504922836304573 28.016298223364984 23.779480876380706 2 22.85759899531439 31.885257849808507 25.124939495836013 20.13220365904109 3 25.463720491748298 24.935965231371018 26.510640300502452 23.08967397637823 4 21.491150732787325 27.27907379433811 24.0713702685952 27.15840520427936 5 23.348310540083663 29.56228736004858 25.65778435043174 21.431617749436022 6 27.545357393346944 26.79471952687955 24.956421050807595 20.70350202896591 7 22.751996354153892 25.457558120213918 29.324716751575195 22.46572877405699 8 26.60783900029494 26.47305593550067 24.765407871101573 22.153697193102815 9 25.737500625897635 25.350340990659593 26.30236434535622 22.609794038086548 10-11 22.29228140390294 28.34279877668586 27.185053902040096 22.1798659173711 12-13 22.141260591804876 26.435805111400423 27.261105295161126 24.161829001633578 14-15 22.146031853725557 30.080087238029552 25.51927335592707 22.25460755231782 16-17 24.168806921347922 26.100507018778472 25.59953671982292 24.131149340050683 18-19 22.19023948613396 28.02086101197222 25.668302547314394 24.120596954579423 20-21 24.07623407764759 28.12907706794825 25.630869889746034 22.163818964658123 22-23 24.027539458642377 26.253508485110327 27.473958039385497 22.2449940168618 24-25 22.31462411023004 27.83641303959857 25.53107770340553 24.317885146765864 26-27 22.370965190679584 26.03990251752572 29.3363886319315 22.252743659863203 28-29 22.45756736804037 26.087766462626206 29.241193177536555 22.21347299179687 30-31 22.372727707250895 26.000774931278244 29.287236108950047 22.339261252520814 32-33 22.446635119118106 26.167242550651398 29.103282715666047 22.282839614564452 34-35 22.444798542210822 26.29178559465445 29.006029517537918 22.25738634559681 36-37 22.387938633045216 26.25750481879667 29.078713605792178 22.27584294236594 38-39 22.447885974647384 26.260759121206657 29.102481731062586 22.188873173083376 40-41 22.465317235585278 26.331421946454398 29.046028244816508 22.15723257314382 42-43 22.49915959770947 26.3310075337679 28.988401787257235 22.1814310812654 44-45 22.53484439852614 26.41452450602672 28.913958996649576 22.136672098797565 46-47 22.55790145749951 26.53417775740994 28.80158093449854 22.106339850592015 48-49 22.577596486877212 26.571424665933733 28.699384380103588 22.151594467085467 50-51 22.633742980849327 26.666812448700828 28.569412420425895 22.13003215002395 52-53 22.709783741908627 26.797566825667076 28.34139218433543 22.15125724808887 54-55 22.852478882336325 26.979952316683647 27.857712270561173 22.309856530418855 56-57 23.11679035813081 27.33135388715357 27.17541238988025 22.376443364835367 58-59 23.182351947197944 27.62750894251343 26.78605761889929 22.404081491389334 60-61 23.179763083969597 27.730194982983885 26.64069057894065 22.44935135410587 62-63 23.125145770067913 27.829464069582926 26.53857921073986 22.506810949609303 64-65 23.103082351697495 27.91495872726125 26.448059785109557 22.533899135931694 66-67 23.055635493617793 27.826652060561248 26.509486973235024 22.60822547258593 68-69 23.00275128570578 27.781484717312644 26.561930896780737 22.65383310020084 70-71 22.955586065212664 27.708029500084546 26.592206281638592 22.744178153064194 72-73 22.913214515555353 27.691670818114776 26.520690853318353 22.87442381301152 74-75 22.929582714787315 27.73448229699375 26.372724634347993 22.96321035387094 76-77 22.919968058081423 27.8026213340228 26.200834884246145 23.076575723649643 78-79 22.937000723077563 27.874086873040394 25.933735178689655 23.255177225192387 80-81 22.962286213860093 27.959690540031957 25.70064278012165 23.3773804659863 82-83 22.948986654920546 28.00536409691495 25.49250511509924 23.553144133065267 84-85 22.91193545481991 27.958672607746752 25.377510537102832 23.751881400330507 86-87 22.92739978012352 27.921593727205305 25.276429438635677 23.874577054035495 88-89 22.89701011867279 27.867128470930037 25.194237930426155 24.04162347997102 90-91 22.894032541196143 27.737968098246352 25.15980876113508 24.208190599422426 92-93 22.893466306045234 27.672547713933216 25.122779556523504 24.311206423498046 94-95 22.85935711502275 27.604699444927594 25.096067877797836 24.43987556225182 96-97 22.819784268089986 27.505631416212605 25.0945843628818 24.57999995281561 98-99 22.798287468053037 27.484328870776697 25.085596801910228 24.63178685926004 100 22.70409933522425 27.493173469333797 25.040991725183176 24.76173547025878 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 46013.0 1 30238.0 2 11442.5 3 7655.0 4 6641.5 5 6293.5 6 6294.5 7 6597.0 8 6713.0 9 6915.5 10 7550.0 11 11256.0 12 92152.0 13 104779.5 14 38155.0 15 38748.0 16 43680.5 17 48311.0 18 52731.0 19 57733.0 20 63924.5 21 73589.0 22 87497.5 23 103387.5 24 119441.0 25 136614.0 26 154858.5 27 177906.0 28 211120.0 29 246088.5 30 273705.0 31 305235.5 32 339729.0 33 363867.5 34 388897.0 35 428176.0 36 468268.5 37 495532.0 38 528511.0 39 567740.5 40 599192.5 41 638874.5 42 689510.0 43 717673.5 44 730715.0 45 758635.0 46 793639.0 47 824149.5 48 851741.5 49 880505.5 50 906399.5 51 933814.0 52 958500.5 53 970843.0 54 963224.5 55 932790.5 56 888944.5 57 825531.5 58 736900.0 59 632391.5 60 521945.5 61 417580.5 62 326641.5 63 248070.0 64 183803.5 65 133962.5 66 95966.5 67 69362.5 68 51058.5 69 38555.0 70 29548.5 71 22854.5 72 18081.0 73 13797.5 74 11327.0 75 10454.5 76 7859.0 77 4011.0 78 1971.5 79 1206.0 80 687.5 81 406.5 82 237.0 83 127.5 84 78.5 85 53.5 86 33.5 87 19.0 88 14.5 89 8.5 90 3.0 91 2.5 92 1.5 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10651343960353214 2 0.07402980985199285 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.989665397868942E-4 20-21 0.002200556435710337 22-23 7.504670284446529E-4 24-25 7.179264526855351E-4 26-27 0.006530486797657942 28-29 0.006412527210531139 30-31 0.007590089295814212 32-33 0.0077446570306700215 34-35 0.006965716998436141 36-37 0.010447558604661738 38-39 0.013343669847223216 40-41 0.016717720796246737 42-43 0.023138383150717656 44-45 0.011675965339568432 46-47 0.01848914838913371 48-49 0.016022165989395595 50-51 0.018775912213010934 52-53 0.021129002597592136 54-55 0.11171383036703614 56-57 0.10494945818110954 58-59 0.10185606969800841 60-61 0.14365850683256504 62-63 0.09486187969578304 64-65 0.07391795162282087 66-67 0.08352352282971545 68-69 0.07689134673281027 70-71 0.0538383825934603 72-73 0.03223754164736095 74-75 0.021181881033200702 76-77 0.01301419651766215 78-79 0.0092171180837701 80-81 0.006015938943466892 82-83 5.043789242663249E-4 84-85 0.0024771513296628377 86-87 0.0017632924489471924 88-89 0.0021883537198006675 90-91 0.0031564358486344205 92-93 0.00488515393583755 94-95 0.0020785292766136456 96-97 0.0011755282992981283 98-99 0.0015883868542419346 100 0.0018751506781191597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4584691E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.71029428374811 #Duplication Level Percentage of deduplicated Percentage of total 1 76.9024012421038 37.459385956297524 2 11.185256152702575 10.896742376744932 3 4.365583572994021 6.37946581682506 4 2.179317792429461 4.246208440281893 5 1.2915211115389882 3.145518670836879 6 0.8366975840614485 2.4453471327680534 7 0.5671972086034647 1.9339840063596656 8 0.42275360044364585 1.6473961829699246 9 0.3225584312445102 1.41407244986618 >10 1.7099634383925797 15.378388581370414 >50 0.14564883104975992 4.817986832432056 >100 0.07006793693404069 6.142537436475144 >500 6.565515227344517E-4 0.21819583967199208 >1k 3.34876821774816E-4 0.2685722219249092 >5k 8.333831489896465E-6 0.0341601357959156 >10k+ 3.333532595958585E-5 3.5720379193795586 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 794360 3.231116470001596 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42756 0.17391310714460476 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0036160714812319586 4.067571969889717E-6 0.0 0.0 8.135143939779433E-6 2 0.0036445444850211864 8.135143939779433E-6 0.0 0.0 8.135143939779433E-6 3 0.0036608147729007452 1.2202715909669152E-5 0.0 0.0 8.135143939779433E-6 4 0.003664882344870635 2.4405431819338304E-5 0.0 0.0 1.6270287879558867E-5 5 0.003664882344870635 4.881086363867661E-5 0.0 4.067571969889717E-6 2.847300378922802E-5 6 0.003681152632750194 5.2878435608566326E-5 0.0 4.067571969889717E-6 3.660814772900745E-5 7 0.0036852202047200837 6.1013579548345756E-5 0.0 8.135143939779433E-6 4.067571969889717E-5 8 0.0036892877766899735 6.914872348812519E-5 0.0 8.135143939779433E-6 4.067571969889717E-5 9 0.0036933553486598633 7.32162954580149E-5 0.0 8.135143939779433E-6 4.067571969889717E-5 10-11 0.0037055580645695323 8.135143939779434E-5 0.0 1.0168929924724293E-5 4.270950568384203E-5 12-13 0.0037177607804792017 1.0168929924724292E-4 0.0 1.2202715909669152E-5 5.084464962362147E-5 14-15 0.0037482675702533745 1.1999337311174665E-4 0.0 1.8304073864503726E-5 6.508115151823547E-5 16-17 0.003782841931997437 1.2812851705152608E-4 0.0 2.0337859849448585E-5 7.32162954580149E-5 18-19 0.0038133487217716098 1.464325909160298E-4 0.0 2.2371645834393445E-5 7.728386742790462E-5 20-21 0.0038682609433651214 1.6473666478053354E-4 0.0 2.4405431819338304E-5 9.558794129240836E-5 22-23 0.004224173490730471 1.7287180872031297E-4 0.0 2.4405431819338304E-5 9.965551326229807E-5 24-25 0.005841033348761634 1.8304073864503726E-4 0.0 2.4405431819338304E-5 1.1999337311174665E-4 26-27 0.011743080277071613 1.9931102652459613E-4 0.0 2.847300378922802E-5 1.2609473106658123E-4 28-29 0.028615368808174158 2.1761510038909986E-4 0.0 3.0506789774172875E-5 1.2812851705152608E-4 30-31 0.06675292359786014 2.2981781629876903E-4 0.0 3.2540575759117734E-5 1.3016230303647094E-4 32-33 0.13750427044212188 2.3795296023854844E-4 0.0 3.2540575759117734E-5 1.3422987500636064E-4 34-35 0.2483374714776769 2.460881041783279E-4 0.0 3.2540575759117734E-5 1.3829744697625037E-4 36-37 0.4058704662995357 2.5829082008799707E-4 0.0 3.457436174406259E-5 1.4439880493108496E-4 38-39 0.610819147574399 2.765948939525008E-4 0.0 3.864193371395231E-5 1.5050016288591954E-4 40-41 0.8647922400163581 2.8269625190733536E-4 0.0 4.474329166878689E-5 1.5456773485580925E-4 42-43 1.1664555800192893 3.0303411175678393E-4 0.0 4.474329166878689E-5 1.5456773485580925E-4 44-45 1.5137957194580969 3.1116925569656334E-4 0.0 4.474329166878689E-5 1.6270287879558868E-4 46-47 1.9093711611018418 3.538787613804054E-4 0.0 4.474329166878689E-5 1.6880423675042327E-4 48-49 2.349966489308326 3.7625040721479884E-4 0.0 4.474329166878689E-5 1.810069526600924E-4 50-51 2.8366758809374497 3.782841931997437E-4 0.0 4.474329166878689E-5 1.9524345455470643E-4 52-53 3.3665340760231643 3.9048690910941286E-4 0.0 4.881086363867661E-5 2.1354752841921016E-4 54-55 3.9344098325254526 4.1082476895886144E-4 0.0 4.881086363867661E-5 2.15581314404155E-4 56-57 4.538004972281327 4.3319641479325487E-4 0.0 5.2878435608566326E-5 2.257502443288793E-4 58-59 5.177223907349497 4.474329166878689E-4 0.0 5.2878435608566326E-5 2.3388538826865873E-4 60-61 5.847992964402115 4.5556806062764835E-4 0.0 5.694600757845604E-5 2.4405431819338305E-4 62-63 6.550377224590701 4.799734924469866E-4 0.0 5.694600757845604E-5 2.6032460607294187E-4 64-65 7.279538311057072 5.145478541910492E-4 0.0 5.89797935634009E-5 2.704935359976662E-4 66-67 8.030786313319943 5.409870719953324E-4 0.0 6.1013579548345756E-5 2.7659489395250074E-4 68-69 8.805361840830134 5.491222159351118E-4 0.0 6.304736553329062E-5 2.9083139584711477E-4 70-71 9.59973627490376 5.531897879050016E-4 0.0 6.508115151823547E-5 2.9896653978689423E-4 72-73 10.410885375781213 5.755614337393949E-4 0.0 6.508115151823547E-5 3.091354697116185E-4 74-75 11.241601531619821 5.938655076038986E-4 0.0 6.508115151823547E-5 3.1116925569656334E-4 76-77 12.089907902442215 6.04034437528623E-4 0.0 7.118250947307005E-5 3.2133818562128766E-4 78-79 12.95330903284487 6.243722973780716E-4 0.0 7.728386742790462E-5 3.437098314556811E-4 80-81 13.831768314680058 6.32507441317851E-4 0.0 8.135143939779434E-5 3.579463333502951E-4 82-83 14.721822210415416 6.508115151823547E-4 0.0 8.33852253827392E-5 3.6404769130512967E-4 84-85 15.619498736022349 6.508115151823547E-4 0.0 8.541901136768406E-5 3.701490492599643E-4 86-87 16.527301482048323 6.589466591221341E-4 0.0 8.745279735262893E-5 3.7218283524490913E-4 88 17.215050618289244 6.670818030619137E-4 0.0 8.948658333757378E-5 3.74216621229854E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 112295 0.0 78.8782 8 GTGGTAT 112965 0.0 78.58918 6 AGTGGTA 113595 0.0 78.29393 5 TGGTATC 113645 0.0 77.97012 7 AAGCAGT 116080 0.0 77.391464 1 GTATCAA 114325 0.0 77.341995 9 GCAGTGG 121405 0.0 73.75644 3 CAGTGGT 121955 0.0 73.21966 4 AGCAGTG 127840 0.0 70.385506 2 CAACGCA 111045 0.0 42.03223 12-13 ATCAACG 112485 0.0 41.491974 10-11 AGAGTAC 113990 0.0 40.56394 18-19 ACGCAGA 115110 0.0 40.528732 14-15 TCAACGC 110525 0.0 39.93051 12-13 AGTACTT 110770 0.0 39.653194 20-21 GAGTACT 104800 0.0 39.58748 20-21 AACGCAG 112755 0.0 39.056572 14-15 TATCAAC 115910 0.0 38.172596 10-11 GTACTTT 109525 0.0 37.7136 22-23 CGCAGAG 116825 0.0 37.618557 16-17 >>END_MODULE