FastQCFastQC Report
Thu 26 May 2016
SRR1660654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33185030
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9277322.7956340554762193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA354430.10680418248830874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1294800.079.329398
GTGGTAT1307150.078.6994556
AGTGGTA1314600.078.360375
TGGTATC1314850.077.960227
GTATCAA1320650.077.589769
AAGCAGT1398800.074.423431
CAGTGGT1443850.071.5757454
GCAGTGG1455550.071.410663
AGCAGTG1544300.067.628572
CAACGCA1257850.042.99374812-13
ATCAACG1282600.042.2580510-11
ACGCAGA1305300.041.441514-15
AGAGTAC1305050.040.9560918-19
TCAACGC1253650.040.71854812-13
AACGCAG1278900.039.82436414-15
GAGTACT1235800.039.5551420-21
AGTACTT1326700.039.1489120-21
TATCAAC1333650.038.4067510-11
CGCAGAG1334350.038.05619416-17
GTACTTT1300300.037.55914322-23
Produced by FastQC (version 0.11.5)