##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660654_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33185030 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37635195749409 34.0 31.0 34.0 31.0 34.0 2 32.53437417413816 34.0 31.0 34.0 31.0 34.0 3 32.55610183266371 34.0 31.0 34.0 31.0 34.0 4 35.91821589433549 37.0 37.0 37.0 35.0 37.0 5 35.91506402133733 37.0 37.0 37.0 35.0 37.0 6 35.92837457130519 37.0 37.0 37.0 35.0 37.0 7 35.89705297840623 37.0 36.0 37.0 35.0 37.0 8 35.905532464487756 37.0 37.0 37.0 35.0 37.0 9 37.63241413372234 39.0 38.0 39.0 35.0 39.0 10-11 37.58327632971855 39.0 38.0 39.0 35.0 39.0 12-13 37.61686072002948 39.0 38.0 39.0 35.0 39.0 14-15 39.09352114492589 41.0 39.0 41.0 36.0 41.0 16-17 39.05335182761625 41.0 39.0 41.0 36.0 41.0 18-19 39.022440012861225 41.0 39.0 41.0 36.0 41.0 20-21 38.965586952912204 41.0 39.0 41.0 35.5 41.0 22-23 38.8548708559251 40.5 39.0 41.0 35.0 41.0 24-25 38.82855745497292 40.0 39.0 41.0 35.0 41.0 26-27 38.77311227381744 40.0 39.0 41.0 35.0 41.0 28-29 38.68390045149876 40.0 38.5 41.0 35.0 41.0 30-31 38.58404982608122 40.0 38.0 41.0 34.0 41.0 32-33 38.476536814943366 40.0 38.0 41.0 34.0 41.0 34-35 38.37392301589 40.0 38.0 41.0 34.0 41.0 36-37 38.233646903438085 40.0 38.0 41.0 33.5 41.0 38-39 37.95012623161709 40.0 37.5 41.0 33.0 41.0 40-41 37.80892468682415 40.0 37.0 41.0 33.0 41.0 42-43 37.655065763086554 40.0 37.0 41.0 33.0 41.0 44-45 37.511989155953756 40.0 37.0 41.0 32.5 41.0 46-47 37.33287565206359 40.0 36.0 41.0 32.0 41.0 48-49 37.35261313610384 40.0 36.0 41.0 32.0 41.0 50-51 36.39455439696755 38.5 35.5 40.0 31.0 40.5 52-53 36.62101988456843 39.0 35.5 39.5 31.0 40.5 54-55 37.13926178460589 40.0 36.0 41.0 31.0 41.0 56-57 36.94066755401457 40.0 36.0 41.0 31.0 41.0 58-59 36.724731784181 39.0 35.0 41.0 31.0 41.0 60-61 36.48616659379245 39.0 35.0 41.0 30.0 41.0 62-63 36.205586404472136 39.0 35.0 41.0 30.0 41.0 64-65 35.89989055305962 37.5 35.0 40.5 29.5 41.0 66-67 35.56899501070212 37.0 35.0 40.0 29.0 41.0 68-69 35.24691387954147 36.5 35.0 39.5 29.0 41.0 70-71 34.9014897379933 36.0 35.0 39.0 29.0 41.0 72-73 34.54693355708885 35.5 34.0 39.0 29.0 41.0 74-75 34.19711062488116 35.0 34.0 37.5 28.0 40.0 76-77 33.8511785434577 35.0 34.0 37.0 28.0 39.0 78-79 33.523498140577246 35.0 34.0 36.5 27.0 39.0 80-81 33.221610723268896 35.0 34.0 36.0 27.0 38.0 82-83 32.96835773540057 35.0 34.0 36.0 26.5 37.0 84-85 32.74460276214908 35.0 33.0 35.0 26.0 37.0 86-87 32.55377234855596 35.0 33.0 35.0 26.0 36.0 88-89 32.37724376021357 35.0 33.0 35.0 25.0 36.0 90-91 32.25556089598231 35.0 33.0 35.0 25.0 36.0 92-93 32.13629980144661 35.0 33.0 35.0 25.0 36.0 94-95 32.036337318363124 35.0 33.0 35.0 25.0 35.0 96-97 31.941200866776377 35.0 33.0 35.0 24.5 35.0 98-99 31.702307094494113 35.0 33.0 35.0 24.0 35.0 100 31.639327702882895 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.528880459099085 1101 2 0.5076732403660031 1101 3 0.4887167265139851 1101 4 0.4275851482749644 1101 5 0.4079839547147017 1101 6 0.37387016829072195 1101 7 0.40244807407363936 1101 8 0.3884462019076196 1101 9 0.47822707537135756 1101 10-11 0.46465447026902496 1101 12-13 0.4664466162998693 1101 14-15 0.6113321836126602 1101 16-17 0.6266928828733782 1101 18-19 0.5861560654861435 1101 20-21 0.5982490978022454 1101 22-23 0.5999182025544414 1101 24-25 0.6256903740089754 1101 26-27 0.5797730252219893 1101 28-29 0.6023447255292851 1101 30-31 0.5980263665511103 1101 32-33 0.6063075766852251 1101 34-35 0.5643733837564469 1101 36-37 0.5823606528141667 1101 38-39 0.5910335557042927 1101 40-41 0.6095141026577977 1101 42-43 0.6108902840871124 1101 44-45 0.593602509975689 1101 46-47 0.5856816408655803 1101 48-49 0.5786882183981774 1101 50-51 0.5245947223297023 1101 52-53 0.6038661065227302 1101 54-55 0.6378183805608941 1101 56-57 0.6259346205656016 1101 58-59 0.5820525309971316 1101 60-61 0.5684200698886457 1101 62-63 0.5669293300104812 1101 64-65 0.5416786944124041 1101 66-67 0.5548632884671889 1101 68-69 0.5291532256754437 1101 70-71 0.4875198100595881 1101 72-73 0.42877381525202196 1101 74-75 0.4257985041500234 1101 76-77 0.4271618521743079 1101 78-79 0.46209963312844593 1101 80-81 0.4395041163767459 1101 82-83 0.39587572535569393 1101 84-85 0.43741096333319973 1101 86-87 0.43647794353413616 1101 88-89 0.419796690951479 1101 90-91 0.41281722710886726 1101 92-93 0.42347105861544776 1101 94-95 0.40042439459982404 1101 96-97 0.3963785006121583 1101 98-99 0.24266103155855134 1101 100 0.3094120001921361 1102 1 0.4752790777473308 1102 2 0.438249469862221 1102 3 0.4628906618202393 1102 4 0.38161738863656325 1102 5 0.3785167687350537 1102 6 0.3444517554836324 1102 7 0.3536060817117175 1102 8 0.39958137024731144 1102 9 0.4751156750335639 1102 10-11 0.4765644147158099 1102 12-13 0.44859016829069276 1102 14-15 0.5531289371317243 1102 16-17 0.5866491181263243 1102 18-19 0.5919287942296023 1102 20-21 0.5553956516007403 1102 22-23 0.5721929175278078 1102 24-25 0.5929042434760348 1102 26-27 0.5740926233018158 1102 28-29 0.5680381910638417 1102 30-31 0.5668450980234283 1102 32-33 0.5892028187546643 1102 34-35 0.5592888703536048 1102 36-37 0.6029513273727432 1102 38-39 0.5939160428350903 1102 40-41 0.6037763250894415 1102 42-43 0.6237382614806748 1102 44-45 0.5683815572348649 1102 46-47 0.5901550514802167 1102 48-49 0.5720292128939732 1102 50-51 0.5881413698946574 1102 52-53 0.6015235982077272 1102 54-55 0.6537916509558528 1102 56-57 0.6270060952525967 1102 58-59 0.6019244767236103 1102 60-61 0.5671684085576203 1102 62-63 0.5462762595002815 1102 64-65 0.5419755598846976 1102 66-67 0.5270618817177208 1102 68-69 0.5247113907997942 1102 70-71 0.5493005745361756 1102 72-73 0.5465127747793233 1102 74-75 0.5316650985848597 1102 76-77 0.4956295578357981 1102 78-79 0.46278665766931937 1102 80-81 0.469900712529828 1102 82-83 0.4497288097465315 1102 84-85 0.47697731887436845 1102 86-87 0.46892238671227204 1102 88-89 0.48222784558638665 1102 90-91 0.4502744605587239 1102 92-93 0.4693470868304175 1102 94-95 0.47308997229617233 1102 96-97 0.4248989857656156 1102 98-99 0.27297615716296875 1102 100 0.3405620087231931 1103 1 0.3984945372952211 1103 2 0.41336186581234813 1103 3 0.39661951408588436 1103 4 0.35568562006864823 1103 5 0.38301397940676196 1103 6 0.34904522833723917 1103 7 0.3548198987605886 1103 8 0.39621145886641074 1103 9 0.45813650193352373 1103 10-11 0.4604410347375847 1103 12-13 0.4237640798587776 1103 14-15 0.5116453526613469 1103 16-17 0.5605068694549757 1103 18-19 0.5381740606590597 1103 20-21 0.5323541715686062 1103 22-23 0.5670866570541833 1103 24-25 0.5648223875348108 1103 26-27 0.536962936810923 1103 28-29 0.5597952758407203 1103 30-31 0.5690931233013927 1103 32-33 0.557865887122567 1103 34-35 0.5573448913945569 1103 36-37 0.5609892740021891 1103 38-39 0.5974624933914043 1103 40-41 0.625245138625921 1103 42-43 0.6131935038129868 1103 44-45 0.5891376455655504 1103 46-47 0.5997194413973972 1103 48-49 0.552046282063813 1103 50-51 0.4812946524763362 1103 52-53 0.5752691378197667 1103 54-55 0.6001420305922167 1103 56-57 0.5726231226604597 1103 58-59 0.5554749289936396 1103 60-61 0.5224244435408139 1103 62-63 0.5127850964407088 1103 64-65 0.5122809675158138 1103 66-67 0.5113846896645313 1103 68-69 0.48098914425521855 1103 70-71 0.488276573460773 1103 72-73 0.43401100659698244 1103 74-75 0.45152903102123787 1103 76-77 0.44767749290284087 1103 78-79 0.4369084025261003 1103 80-81 0.4355283243072776 1103 82-83 0.42098015464775074 1103 84-85 0.40527042956369996 1103 86-87 0.408452048139182 1103 88-89 0.43808691244242226 1103 90-91 0.43619901331634736 1103 92-93 0.42559286030999743 1103 94-95 0.43823183702406254 1103 96-97 0.44135956381038 1103 98-99 0.29881476199053836 1103 100 0.33450769781353884 1104 1 0.3694630774218055 1104 2 0.3710286377402525 1104 3 0.3580535398263649 1104 4 0.31495029561444454 1104 5 0.30152147747433844 1104 6 0.27982095658251893 1104 7 0.30395144958458786 1104 8 0.32250564695615935 1104 9 0.37718555994216985 1104 10-11 0.3992356051302437 1104 12-13 0.37896123379093893 1104 14-15 0.47798694501089756 1104 16-17 0.4688860734692284 1104 18-19 0.4626460790969773 1104 20-21 0.4731389073603438 1104 22-23 0.48469132768850187 1104 24-25 0.4769618818980419 1104 26-27 0.47779178495692776 1104 28-29 0.4823268720129974 1104 30-31 0.5060004162825962 1104 32-33 0.47506521166508975 1104 34-35 0.4987267248126699 1104 36-37 0.4750237030864852 1104 38-39 0.4836464810316201 1104 40-41 0.4748891811185203 1104 42-43 0.4891644156677728 1104 44-45 0.4494201014517998 1104 46-47 0.4709844883045875 1104 48-49 0.46866918934171764 1104 50-51 0.36046026641399465 1104 52-53 0.4499066251552577 1104 54-55 0.5323185851838375 1104 56-57 0.4720252531772786 1104 58-59 0.42798418057387266 1104 60-61 0.43088031316404596 1104 62-63 0.4431641735474017 1104 64-65 0.4187026631500572 1104 66-67 0.39621357728600515 1104 68-69 0.3822165386020444 1104 70-71 0.3780785392567978 1104 72-73 0.3577658432912827 1104 74-75 0.3469641925888922 1104 76-77 0.3486998863816524 1104 78-79 0.38382731091463995 1104 80-81 0.42816532416531317 1104 82-83 0.41392894640155475 1104 84-85 0.3879503465606149 1104 86-87 0.33846172787865925 1104 88-89 0.353267647652558 1104 90-91 0.35061341498124676 1104 92-93 0.35802841949267616 1104 94-95 0.3711180685707802 1104 96-97 0.36841436840423825 1104 98-99 0.22444282915246916 1104 100 0.2600735376144492 1105 1 0.3055462400065423 1105 2 0.3137659047110972 1105 3 0.299278710199097 1105 4 0.30323354138528913 1105 5 0.3129756228228331 1105 6 0.2807546650445545 1105 7 0.2641881087298401 1105 8 0.27682231786683786 1105 9 0.33800048770851276 1105 10-11 0.3193671979234409 1105 12-13 0.3493556099107096 1105 14-15 0.4316852799043289 1105 16-17 0.43152267839880665 1105 18-19 0.4233686815433231 1105 20-21 0.39163211989333746 1105 22-23 0.4315916989369626 1105 24-25 0.4182434495498839 1105 26-27 0.4087229568055122 1105 28-29 0.40599388676550774 1105 30-31 0.41904486400380137 1105 32-33 0.4092747195632782 1105 34-35 0.42322138652910724 1105 36-37 0.41016502559399726 1105 38-39 0.4322621128478161 1105 40-41 0.4350019365585709 1105 42-43 0.4174115213046221 1105 44-45 0.43307646507548725 1105 46-47 0.40621292688792465 1105 48-49 0.39984985904812476 1105 50-51 0.3401259651870916 1105 52-53 0.39174178388373804 1105 54-55 0.44584215350979406 1105 56-57 0.42587678213881475 1105 58-59 0.4374037132909052 1105 60-61 0.4009599477401977 1105 62-63 0.4169384722983267 1105 64-65 0.431015781027547 1105 66-67 0.4291207759838187 1105 68-69 0.40573475330054976 1105 70-71 0.38942517628644424 1105 72-73 0.38441111680764806 1105 74-75 0.42169552774641517 1105 76-77 0.4068055079819004 1105 78-79 0.42281445370482373 1105 80-81 0.41179744118798567 1105 82-83 0.3719036030299634 1105 84-85 0.3767431754652364 1105 86-87 0.3790936530246256 1105 88-89 0.4113104066730884 1105 90-91 0.4156095071172885 1105 92-93 0.3999259220599072 1105 94-95 0.39471214655033293 1105 96-97 0.36682886547721694 1105 98-99 0.24246073820521374 1105 100 0.255800426695302 1106 1 0.35875672090305244 1106 2 0.34938357623411065 1106 3 0.3674161962279072 1106 4 0.3148332626754282 1106 5 0.327826734408454 1106 6 0.3222335221931871 1106 7 0.3365867245909584 1106 8 0.3492785061535173 1106 9 0.4093556673509582 1106 10-11 0.4090143615409687 1106 12-13 0.4051455304011782 1106 14-15 0.45052009844785346 1106 16-17 0.49930335804027237 1106 18-19 0.5002426419428829 1106 20-21 0.5204840743154264 1106 22-23 0.49972703798755447 1106 24-25 0.4833539282416197 1106 26-27 0.47800065110651957 1106 28-29 0.5115323533839771 1106 30-31 0.5184360044172607 1106 32-33 0.5217464434155517 1106 34-35 0.5072922816893808 1106 36-37 0.5267162766487203 1106 38-39 0.5582856683366586 1106 40-41 0.5547204882292078 1106 42-43 0.54949552098536 1106 44-45 0.5255686800772068 1106 46-47 0.5591292899161147 1106 48-49 0.564403705666713 1106 50-51 0.5374005938075115 1106 52-53 0.5079720944440922 1106 54-55 0.5684289454916254 1106 56-57 0.5947800526931672 1106 58-59 0.5608176134568623 1106 60-61 0.5677441447893443 1106 62-63 0.5404266466137386 1106 64-65 0.5557433266080452 1106 66-67 0.5491245807200471 1106 68-69 0.5474775344609668 1106 70-71 0.5090763400013927 1106 72-73 0.524192729813187 1106 74-75 0.49899442098220703 1106 76-77 0.4719776439460759 1106 78-79 0.4815825640669402 1106 80-81 0.48790442081117646 1106 82-83 0.4922072966975293 1106 84-85 0.5453531861012877 1106 86-87 0.533336115503289 1106 88-89 0.500105191698232 1106 90-91 0.5371914784438161 1106 92-93 0.5393377656887353 1106 94-95 0.5397780636126299 1106 96-97 0.49049279276312063 1106 98-99 0.42436192602604805 1106 100 0.43745682895537286 1107 1 0.31756351679596406 1107 2 0.283229484286899 1107 3 0.26671235937813975 1107 4 0.2775372338855959 1107 5 0.31262779306038624 1107 6 0.24445870908255785 1107 7 0.2339155142736189 1107 8 0.26221568660378125 1107 9 0.3332869489834991 1107 10-11 0.36905793746682747 1107 12-13 0.3272516151758751 1107 14-15 0.3713048065371751 1107 16-17 0.3836745261943051 1107 18-19 0.4130041257175989 1107 20-21 0.40417872481430805 1107 22-23 0.4022182520080335 1107 24-25 0.4096778531658387 1107 26-27 0.4177145279578127 1107 28-29 0.39098370069630306 1107 30-31 0.43222860542266517 1107 32-33 0.42814692966175727 1107 34-35 0.43940314928592983 1107 36-37 0.4092771382433824 1107 38-39 0.42145092647066207 1107 40-41 0.44501259515475056 1107 42-43 0.42811190393360476 1107 44-45 0.4362190515158133 1107 46-47 0.4477108758009223 1107 48-49 0.4237008951527912 1107 50-51 0.31736956151134876 1107 52-53 0.39101437195778743 1107 54-55 0.4783133131150592 1107 56-57 0.4038506306720251 1107 58-59 0.43760250320808325 1107 60-61 0.43445502823358595 1107 62-63 0.423167371931342 1107 64-65 0.3865639248382138 1107 66-67 0.38162068647204705 1107 68-69 0.4100315887847188 1107 70-71 0.3422397157947614 1107 72-73 0.35928994856455887 1107 74-75 0.3756358243573743 1107 76-77 0.3773455029214148 1107 78-79 0.38312970625614184 1107 80-81 0.3742715800899319 1107 82-83 0.36104866129254987 1107 84-85 0.4141406254293898 1107 86-87 0.40617171082089953 1107 88-89 0.40321217896704553 1107 90-91 0.35880533410945503 1107 92-93 0.4235632432258214 1107 94-95 0.3952146916480501 1107 96-97 0.3736610376066807 1107 98-99 0.23517881324341872 1107 100 0.2660517637427269 1108 1 0.1436769130825155 1108 2 0.12358463191463898 1108 3 0.13537659534257784 1108 4 0.1705528519508448 1108 5 0.1437024452840845 1108 6 0.1410455297755604 1108 7 0.14016530742501487 1108 8 0.13702518139265152 1108 9 0.17718182458122556 1108 10-11 0.16194274937770103 1108 12-13 0.13676682053817757 1108 14-15 0.14842226371709444 1108 16-17 0.2030801414190293 1108 18-19 0.16724767815430397 1108 20-21 0.1841681521694838 1108 22-23 0.18649611559474266 1108 24-25 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quality scores pass #Quality Count 2 915.0 3 299.0 4 329.0 5 235.0 6 863.0 7 7270.0 8 14891.0 9 18203.0 10 19921.0 11 20403.0 12 21697.0 13 24991.0 14 30498.0 15 39851.0 16 53202.0 17 67819.0 18 76076.0 19 85325.0 20 99640.0 21 119377.0 22 151567.0 23 211920.0 24 295006.0 25 373235.0 26 398201.0 27 377673.0 28 347712.0 29 366742.0 30 426001.0 31 520513.0 32 657535.0 33 864150.0 34 1235151.0 35 2001835.0 36 3871950.0 37 8155454.0 38 9696846.0 39 2527371.0 40 4363.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.799774026683487 29.333257413444386 28.33845339961176 24.528515160260365 2 23.21113957711032 31.204337886636985 25.04788543854849 20.5366370977042 3 25.15607737050451 24.62865778811152 26.756465110940848 23.45879973044312 4 21.203386852128553 27.159883614767327 23.896542197668584 27.740187335435536 5 23.568877267057974 28.935160214060463 25.924912727950574 21.571049790930992 6 27.797270949286823 27.168768971637352 24.611091424254443 20.42286865482138 7 23.1917426356161 25.130927840708296 28.930528998824627 22.74680052485098 8 26.401146871765985 26.636200473556116 24.70220197654613 22.260450678131765 9 25.322982943693546 25.073587997885564 26.669942938253122 22.933486120167764 10-11 22.677624023486302 27.944215209202532 26.98456988848998 22.393590878821186 12-13 22.455751907336634 26.301766246804092 27.11516638624007 24.12731545961921 14-15 22.44918803382484 29.414887323377414 25.693413610450445 22.442511032347305 16-17 24.19623758298193 25.945883891743186 25.736072751004897 24.121805774269987 18-19 22.45846334091092 27.575760210634016 25.862733607705437 24.103042840749627 20-21 24.054380656001833 27.781615645398617 25.793066052674828 22.370937645924723 22-23 24.16107430086486 26.107511765729225 27.284678444793453 22.446735488612468 24-25 22.61083825648592 27.49436390614179 25.626852596204802 24.267945241167492 26-27 22.62103429364385 25.987743458807426 28.92514878768675 22.466073459861978 28-29 22.68828439918575 25.992942203438645 28.876645223952725 22.44212817342288 30-31 22.566230796838877 25.864735321462657 28.988143674219437 22.580890207479033 32-33 22.71556402431697 26.033910430657375 28.76495589143791 22.485569653587753 34-35 22.729951767575916 26.2161460785102 28.5888664723354 22.46503568157848 36-37 22.597629520178884 26.165361599619196 28.757250145765738 22.479758734436178 38-39 22.650548656933573 26.11380177770907 28.775678776366696 22.459970788990656 40-41 22.73715941501986 26.151419884974096 28.673310794665646 22.438109905340397 42-43 22.757159187741255 26.163915222459938 28.68239013364456 22.396535456154247 44-45 22.88635601778577 26.249488180424652 28.570976235340567 22.293179566449016 46-47 22.838002875701527 26.33528455331114 28.511757016558757 22.31495555442858 48-49 22.94532648199149 26.334097331341923 28.38444072354281 22.336135463123778 50-51 23.027127207643485 26.381257998014824 28.321743130148626 22.26987166419307 52-53 23.11073861458922 26.51529821916489 28.171316183791717 22.20264698245417 54-55 23.33061919441375 26.61691053426901 27.74135767188731 22.311112599429926 56-57 23.66801463748881 26.959491801571133 27.154208076071995 22.218285484868062 58-59 23.867716743241093 27.207231580833096 26.786640023670472 22.13841165225534 60-61 24.00696983835907 27.379655450543805 26.558542844431905 22.05483186666522 62-63 24.121519705592494 27.48848460686321 26.455957947761817 21.93403773978248 64-65 24.2818612925921 27.537733710569963 26.39264159485968 21.78776340197826 66-67 24.38686659916809 27.420067584778696 26.49807485590091 21.6949909601523 68-69 24.54343281662929 27.358123408188597 26.55885315852698 21.539590616655133 70-71 24.702437348337583 27.252456517436286 26.619654695772027 21.42545143845411 72-73 24.849822049531 27.210523430485484 26.616529698382784 21.323124821600732 74-75 25.06673862024847 27.26620960707234 26.496175446153735 21.170876326525462 76-77 25.303057505984867 27.305454270131683 26.307461250722714 21.084026973160736 78-79 25.524642550421927 27.327397453140623 26.130858851317335 21.01710114512012 80-81 25.771005714804186 27.395148332153457 25.92397322450633 20.909872728536026 82-83 25.91346259786924 27.422795160626993 25.763815911817005 20.899926329686753 84-85 26.04262449541007 27.390712282055922 25.704889982570577 20.861773239963423 86-87 26.227969215516033 27.373783062558964 25.6172559682631 20.780991753661908 88-89 26.3557873152433 27.357240940518356 25.53207536965885 20.75489637457949 90-91 26.480766879655132 27.293882371890728 25.51129357469972 20.71405717375442 92-93 26.58108770913701 27.329325321449367 25.495761169018273 20.593825800395354 94-95 26.63377490887932 27.373096404238485 25.44003844231225 20.55309024456995 96-97 26.66208715454839 27.40975912661542 25.39684470348993 20.531309015346263 98-99 26.72810826574735 27.520735273857667 25.339246393871168 20.41191006652382 100 26.726420142209626 27.609787542639197 25.24242664760157 20.421365667549605 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40378.0 1 26766.5 2 10651.0 3 7603.0 4 6953.0 5 6817.5 6 6961.0 7 7217.0 8 7133.5 9 7318.5 10 7951.0 11 11610.5 12 99292.5 13 114930.5 14 44381.5 15 44741.5 16 49640.0 17 54498.0 18 59327.0 19 65024.0 20 72100.0 21 83115.5 22 99959.5 23 119587.5 24 139894.0 25 162889.0 26 187663.5 27 218850.5 28 262178.0 29 307416.0 30 344296.0 31 386471.0 32 433524.0 33 471092.0 34 509000.5 35 563215.0 36 623872.5 37 669606.0 38 722049.5 39 780915.5 40 826798.5 41 883947.0 42 954518.0 43 999526.5 44 1028319.0 45 1075086.0 46 1132763.0 47 1188334.0 48 1235008.5 49 1271512.5 50 1299373.0 51 1318541.5 52 1326054.0 53 1315624.5 54 1284112.0 55 1230591.0 56 1160426.5 57 1072415.5 58 960629.5 59 827253.0 60 684113.5 61 551306.5 62 433963.5 63 329525.5 64 244055.0 65 179482.0 66 130896.0 67 96208.5 68 71838.0 69 55232.5 70 43388.0 71 34704.0 72 28545.5 73 22816.5 74 18438.0 75 15754.5 76 12094.0 77 7513.5 78 4693.0 79 3336.0 80 2285.5 81 1653.0 82 1173.0 83 829.0 84 651.0 85 517.0 86 440.0 87 365.0 88 313.0 89 262.0 90 225.5 91 188.5 92 145.0 93 120.5 94 105.0 95 93.5 96 77.0 97 54.0 98 36.5 99 29.0 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1351030871450169 2 0.050640303775527695 3 0.018481224817334805 4 0.07579622498457889 5 0.07943642057879713 6 0.08130171948013909 7 0.04820245755390307 8 0.04565010186822191 9 0.03357538022415529 10-11 0.022793410161147963 12-13 0.01484856274048871 14-15 0.011957198773061227 16-17 0.017076977179167837 18-19 0.016252810378655676 20-21 0.02292449336342321 22-23 0.019484689331303904 24-25 0.016811797367668496 26-27 0.015166477173593032 28-29 0.01715984587026138 30-31 0.015002246494880373 32-33 0.012055134498899053 34-35 0.009648929050237412 36-37 0.007556117924256811 38-39 0.016322118738479368 40-41 0.025362339585047834 42-43 0.0255642378506212 44-45 0.024274499676510762 46-47 0.026450179493584906 48-49 0.022864225224446085 50-51 0.02307215030391716 52-53 0.019572078132820733 54-55 0.02455323981927996 56-57 0.03119026862413564 58-59 0.02359648311301813 60-61 0.023474440131589454 62-63 0.030726203954011797 64-65 0.03149010261554683 66-67 0.0242066980201615 68-69 0.024721990608415907 70-71 0.03570284553004773 72-73 0.04007680571631245 74-75 0.04111643111366782 76-77 0.042357954776596554 78-79 0.03656618662089502 80-81 0.032137985109550904 82-83 0.034831970921828305 84-85 0.03818739955937964 86-87 0.03817835933853307 88-89 0.03751842321673357 90-91 0.04025459672629496 92-93 0.033578393631104146 94-95 0.03365071539787669 96-97 0.03372153046117481 98-99 0.31346212433738946 100 0.14898283955144834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.318503E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.15545723928229 #Duplication Level Percentage of deduplicated Percentage of total 1 74.83838640480957 33.79361557159263 2 11.759188663056715 10.619830816866212 3 4.6178027587328 6.2555698503419634 4 2.432774239432383 4.394121325660659 5 1.4839414413988037 3.3504027151341305 6 0.9670270193877787 2.6199928333917266 7 0.7045528624143848 2.2270084656096683 8 0.5086489481253161 1.8374620661502903 9 0.3917512547595299 1.592073632945622 >10 2.0215991649006595 16.418670410732283 >50 0.17298958396682276 5.519645774102326 >100 0.09995673673066946 7.640673965754785 >500 9.765449449494387E-4 0.2927938164233718 >1k 3.6438419547396285E-4 0.28840822509231523 >5k 6.665524032411415E-6 0.019308173984883017 >10k+ 3.332762012955359E-5 3.1304223562170477 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 927732 2.7956340554762193 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35443 0.10680418248830874 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.10935352476704104 1.506703474428078E-5 0.0 3.013406948856156E-6 3.013406948856156E-6 2 0.1093565381739899 3.013406948856156E-5 0.0 3.013406948856156E-6 6.026813897712312E-6 3 0.10935955158093875 3.616088338627387E-5 0.0 3.013406948856156E-6 6.026813897712312E-6 4 0.10935955158093875 4.218769728398618E-5 0.0 3.013406948856156E-6 6.026813897712312E-6 5 0.10936859180178532 5.725473202826696E-5 0.0 3.013406948856156E-6 1.506703474428078E-5 6 0.10936859180178532 6.328154592597927E-5 0.0 3.013406948856156E-6 1.506703474428078E-5 7 0.10937160520873418 6.328154592597927E-5 0.0 3.013406948856156E-6 2.109384864199309E-5 8 0.10937160520873418 6.629495287483543E-5 0.0 3.013406948856156E-6 2.4107255590849248E-5 9 0.10937461861568304 7.53351737214039E-5 0.0 3.013406948856156E-6 2.7120662539705405E-5 10-11 0.10938667224347845 9.040220846568468E-5 0.0 7.53351737214039E-6 2.7120662539705405E-5 12-13 0.10940776609212044 9.793572583782507E-5 0.0 1.0546924320996547E-5 3.164077296298964E-5 14-15 0.1094258465338136 1.1149605710767776E-4 0.0 1.2053627795424624E-5 3.616088338627387E-5 16-17 0.10945748730677658 1.2656309185195855E-4 0.0 1.2053627795424624E-5 4.520110423284234E-5 18-19 0.10947858115541857 1.4615023701952358E-4 0.0 1.2053627795424624E-5 5.42413250794108E-5 20-21 0.10952679566660027 1.7477760303365704E-4 0.0 1.506703474428078E-5 6.930835982369159E-5 22-23 0.10972417382175034 1.988848586245063E-4 0.0 1.506703474428078E-5 7.985528414468813E-5 24-25 0.1107622925156313 2.0792507947107477E-4 0.0 1.506703474428078E-5 9.642902236339699E-5 26-27 0.11504585049343033 2.184720037920713E-4 0.0 1.506703474428078E-5 9.642902236339699E-5 28-29 0.1291049006133187 2.3956585243406438E-4 0.0 1.506703474428078E-5 9.944242931225315E-5 30-31 0.15980247720131638 2.591529976016294E-4 0.0 1.8080441693136937E-5 1.16016167530962E-4 32-33 0.21590608777512027 2.727133288714821E-4 0.0 1.8080441693136937E-5 1.2053627795424623E-4 34-35 0.30971947290691015 3.073675087833279E-4 0.0 1.8080441693136937E-5 1.3258990574967086E-4 36-37 0.44963798435619917 3.1791443310432446E-4 0.0 1.8080441693136937E-5 1.446435335450955E-4 38-39 0.6424628816065557 3.269546539508929E-4 0.0 2.260055211642117E-5 1.4615023701952358E-4 40-41 0.8872871291663742 3.359948747974614E-4 0.0 2.7120662539705405E-5 1.4765694049395165E-4 42-43 1.1777268244145025 3.4202168869517367E-4 0.0 2.7120662539705405E-5 1.506703474428078E-4 44-45 1.5155207031604312 3.5106190954174217E-4 0.0 2.862736601413348E-5 1.6121727176380434E-4 46-47 1.910891748478154 3.7818257208144755E-4 0.0 3.616088338627387E-5 1.777910099825132E-4 48-49 2.365780594442735 3.87222792928016E-4 0.0 4.218769728398618E-5 1.9737815515007823E-4 50-51 2.870577486294272 4.007831241978687E-4 0.0 4.6707807707270416E-5 2.0189826557336245E-4 52-53 3.418081888128472 4.083166415700091E-4 0.0 4.9721214656126574E-5 2.2299211421535553E-4 54-55 4.005289433217327 4.1886356589100566E-4 0.0 5.122791813055465E-5 2.3353903853635206E-4 56-57 4.632078078579408 4.429708214818549E-4 0.0 5.122791813055465E-5 2.501127767550609E-4 58-59 5.304280273364225 4.5954455970056377E-4 0.0 5.42413250794108E-5 2.576462941272013E-4 60-61 6.017523564088988 4.776250013937007E-4 0.0 5.725473202826696E-5 2.6216640455048554E-4 62-63 6.758472118301535 5.017322569845499E-4 0.0 5.725473202826696E-5 2.6969992192262595E-4 64-65 7.524187864226731 5.183059952032588E-4 0.0 6.026813897712312E-5 2.7120662539705404E-4 66-67 8.315510035699832 5.273462160498273E-4 0.0 6.328154592597927E-5 2.7723343929476636E-4 68-69 9.13585884960779 5.273462160498273E-4 0.0 6.328154592597927E-5 2.847669566669067E-4 70-71 9.980088913585433 5.363864368963958E-4 0.0 6.328154592597927E-5 2.9682058446233134E-4 72-73 10.84269774654415 5.499467681662484E-4 0.0 6.478824940040735E-5 2.9832728793675943E-4 74-75 11.722730098481152 5.680272098593853E-4 0.0 6.629495287483543E-5 3.028473983600437E-4 76-77 12.619116209929597 5.830942446036662E-4 0.0 6.629495287483543E-5 3.0586080530889984E-4 78-79 13.529501103358953 5.951478723990908E-4 0.0 6.629495287483543E-5 3.1188761920661215E-4 80-81 14.453532210156204 6.072015001945155E-4 0.0 6.780165634926351E-5 3.254479504764648E-4 82-83 15.39432237969952 6.282953488365086E-4 0.0 6.930835982369159E-5 3.2996806089974904E-4 84-85 16.341672736170494 6.403489766319331E-4 0.0 6.930835982369159E-5 3.344881713230333E-4 86-87 17.297469672319117 6.56922714850642E-4 0.0 7.53351737214039E-5 3.4051498522074563E-4 88 18.017482581754482 6.629495287483543E-4 0.0 7.53351737214039E-5 3.4352839216960176E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 129480 0.0 79.32939 8 GTGGTAT 130715 0.0 78.699455 6 AGTGGTA 131460 0.0 78.36037 5 TGGTATC 131485 0.0 77.96022 7 GTATCAA 132065 0.0 77.58976 9 AAGCAGT 139880 0.0 74.42343 1 CAGTGGT 144385 0.0 71.575745 4 GCAGTGG 145555 0.0 71.41066 3 AGCAGTG 154430 0.0 67.62857 2 CAACGCA 125785 0.0 42.993748 12-13 ATCAACG 128260 0.0 42.25805 10-11 ACGCAGA 130530 0.0 41.4415 14-15 AGAGTAC 130505 0.0 40.95609 18-19 TCAACGC 125365 0.0 40.718548 12-13 AACGCAG 127890 0.0 39.824364 14-15 GAGTACT 123580 0.0 39.55514 20-21 AGTACTT 132670 0.0 39.14891 20-21 TATCAAC 133365 0.0 38.40675 10-11 CGCAGAG 133435 0.0 38.056194 16-17 GTACTTT 130030 0.0 37.559143 22-23 >>END_MODULE