FastQCFastQC Report
Thu 26 May 2016
SRR1660654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33185030
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8820862.658084081888731No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG333710.10056040329027877TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1222650.078.125868
GTGGTAT1234400.077.462126
AGTGGTA1244500.076.893865
TGGTATC1242750.076.726197
GTATCAA1245300.076.508729
AAGCAGT1324950.072.961851
GCAGTGG1378900.069.930573
CAGTGGT1376800.069.842754
AGCAGTG1468300.065.906982
CAACGCA1187850.042.55476412-13
ATCAACG1207250.041.90186710-11
ACGCAGA1235500.041.00400514-15
AGAGTAC1246000.040.2947118-19
TCAACGC1183400.040.1423712-13
GAGTACT1169350.039.13029520-21
AACGCAG1212700.039.1251314-15
AGTACTT1263400.038.54544420-21
CGCAGAG1263400.037.55051416-17
TATCAAC1269300.037.5458510-11
GTACTTT1228500.037.18097322-23
Produced by FastQC (version 0.11.5)