##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660654_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33185030 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78492476878882 34.0 31.0 34.0 31.0 34.0 2 32.96798435921257 34.0 31.0 34.0 31.0 34.0 3 33.01969758653224 34.0 33.0 34.0 31.0 34.0 4 36.39771559646021 37.0 37.0 37.0 35.0 37.0 5 36.324314125977885 37.0 37.0 37.0 35.0 37.0 6 36.313677974677134 37.0 37.0 37.0 35.0 37.0 7 36.27355530490706 37.0 37.0 37.0 35.0 37.0 8 36.28441212799868 37.0 37.0 37.0 35.0 37.0 9 38.06150987357854 39.0 38.0 39.0 35.0 39.0 10-11 38.01920777229974 39.0 38.0 39.0 35.0 39.0 12-13 38.04392667416603 39.0 38.0 39.0 35.0 39.0 14-15 39.55417213725586 41.0 40.0 41.0 37.0 41.0 16-17 39.52963634506282 41.0 39.5 41.0 37.0 41.0 18-19 39.499348712356145 41.0 39.0 41.0 37.0 41.0 20-21 39.46845436029439 41.0 39.0 41.0 36.5 41.0 22-23 39.3615440757474 41.0 39.0 41.0 36.0 41.0 24-25 39.34574387909247 41.0 39.0 41.0 36.0 41.0 26-27 39.27862121866396 41.0 39.0 41.0 36.0 41.0 28-29 39.19845593027941 40.5 39.0 41.0 36.0 41.0 30-31 39.110103079611505 40.0 39.0 41.0 36.0 41.0 32-33 39.016604113360756 40.0 39.0 41.0 35.0 41.0 34-35 38.91852246329143 40.0 38.0 41.0 35.0 41.0 36-37 38.78677489518617 40.0 38.0 41.0 35.0 41.0 38-39 38.543289805674426 40.0 38.0 41.0 35.0 41.0 40-41 38.42332719000103 40.0 38.0 41.0 34.5 41.0 42-43 38.28511734055988 40.0 38.0 41.0 34.0 41.0 44-45 38.158156072180745 40.0 37.5 41.0 34.0 41.0 46-47 38.01703784808994 40.0 37.0 41.0 33.5 41.0 48-49 37.85597900619647 40.0 37.0 41.0 33.0 41.0 50-51 37.905715920702804 40.0 37.0 41.0 33.0 41.0 52-53 37.83658024416431 40.0 37.0 41.0 33.0 41.0 54-55 37.456711083280624 40.0 36.0 41.0 33.0 41.0 56-57 37.22785466820431 40.0 36.0 41.0 32.5 41.0 58-59 37.00970048844313 39.0 35.5 41.0 32.0 41.0 60-61 36.76911646606919 39.0 35.0 41.0 32.0 41.0 62-63 36.49773830248157 38.5 35.0 40.5 31.0 41.0 64-65 36.2104786254525 37.5 35.0 40.0 31.0 41.0 66-67 35.917514403331865 37.0 35.0 40.0 31.0 41.0 68-69 35.60582750716212 37.0 35.0 39.5 31.0 41.0 70-71 35.27113269145757 36.0 35.0 39.0 30.5 41.0 72-73 34.91350101235406 35.5 35.0 39.0 30.0 40.5 74-75 34.56704179565304 35.0 34.0 37.5 30.0 39.5 76-77 32.73250195946787 34.5 32.0 35.5 27.0 38.5 78-79 33.88613975940356 35.0 34.0 36.0 29.5 39.0 80-81 33.869852897526386 35.0 34.0 36.0 30.0 38.0 82-83 33.66309420241597 35.0 34.0 36.0 30.0 37.0 84-85 33.45398330210942 35.0 34.0 35.0 30.0 37.0 86-87 33.28066958203745 35.0 34.0 35.0 29.5 36.5 88-89 33.13159843459536 35.0 34.0 35.0 29.0 36.0 90-91 33.03281443470143 35.0 34.0 35.0 29.0 36.0 92-93 32.94744768951542 35.0 34.0 35.0 29.0 36.0 94-95 32.85624100686364 35.0 34.0 35.0 29.0 35.0 96-97 32.787575030066265 35.0 34.0 35.0 29.0 35.0 98-99 32.69974069633206 35.0 34.0 35.0 29.0 35.0 100 32.653336459240805 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35088189594385 1101 2 0.306162228895424 1101 3 0.3065264597333055 1101 4 0.21448010575324616 1101 5 0.20041527845754104 1101 6 0.22272218902896412 1101 7 0.2321070206685505 1101 8 0.23530330096087937 1101 9 0.29914134672996795 1101 10-11 0.2900336135549182 1101 12-13 0.3149264982064892 1101 14-15 0.41142815781802256 1101 16-17 0.4085624327118538 1101 18-19 0.4018601754934963 1101 20-21 0.39731977457415724 1101 22-23 0.389512915647245 1101 24-25 0.4106758946631359 1101 26-27 0.44619113867717886 1101 28-29 0.4474289909217717 1101 30-31 0.4383074381780645 1101 32-33 0.4283699360572939 1101 34-35 0.44344135252758576 1101 36-37 0.4556142291590177 1101 38-39 0.4509247338782245 1101 40-41 0.4571408233795822 1101 42-43 0.4519633595691701 1101 44-45 0.44361376054733626 1101 46-47 0.4088007140070289 1101 48-49 0.4298559853907591 1101 50-51 0.40530012449121244 1101 52-53 0.44996321131296213 1101 54-55 0.35392586679132165 1101 56-57 0.34268129846345374 1101 58-59 0.3554378115878407 1101 60-61 0.3053841565346076 1101 62-63 0.23132149213113706 1101 64-65 0.2674676742234965 1101 66-67 0.2508462764696162 1101 68-69 0.24152690717773595 1101 70-71 0.25927690257318403 1101 72-73 0.2876435320610611 1101 74-75 0.2851486151679765 1101 76-77 0.307962556488512 1101 78-79 0.35540886470347743 1101 80-81 0.3100429062760597 1101 82-83 0.31197439967198903 1101 84-85 0.2894039819360117 1101 86-87 0.31737963346676423 1101 88-89 0.33060175458798113 1101 90-91 0.2868317096237192 1101 92-93 0.27481089364403033 1101 94-95 0.2900476385021449 1101 96-97 0.28366116196959723 1101 98-99 0.26965859628426614 1101 100 0.2783118558882762 1102 1 0.31889577008389836 1102 2 0.27387755811456316 1102 3 0.29140069293796245 1102 4 0.2087102735700057 1102 5 0.2045175910688144 1102 6 0.2140407176744077 1102 7 0.22722143848084642 1102 8 0.22480205606395032 1102 9 0.2682895884269456 1102 10-11 0.2888306840492447 1102 12-13 0.30598504083840794 1102 14-15 0.3900878244566428 1102 16-17 0.40456851971324426 1102 18-19 0.3890110501509412 1102 20-21 0.38178839688080046 1102 22-23 0.40797211054874083 1102 24-25 0.38361193650855796 1102 26-27 0.4055794158311272 1102 28-29 0.4091274353524099 1102 30-31 0.3925214108176931 1102 32-33 0.41344005807397366 1102 34-35 0.4191611375930506 1102 36-37 0.41291154466940583 1102 38-39 0.41394385941504197 1102 40-41 0.44979460689616246 1102 42-43 0.42207997254259055 1102 44-45 0.4442763556511906 1102 46-47 0.4140132992747567 1102 48-49 0.405316165588836 1102 50-51 0.39995074084133364 1102 52-53 0.4120582183678465 1102 54-55 0.3296691588750704 1102 56-57 0.3330727609465569 1102 58-59 0.31835012148614084 1102 60-61 0.2467016612726951 1102 62-63 0.28083554059102767 1102 64-65 0.2849935678029212 1102 66-67 0.2529687950579671 1102 68-69 0.2806481419276068 1102 70-71 0.29033898437619854 1102 72-73 0.30918232302404647 1102 74-75 0.30987495375166674 1102 76-77 0.29485115224152736 1102 78-79 0.3775212670571477 1102 80-81 0.32705662440931604 1102 82-83 0.33010383476200644 1102 84-85 0.37251905586092704 1102 86-87 0.3644147811839602 1102 88-89 0.38220904029169134 1102 90-91 0.36301469248203233 1102 92-93 0.31337555656561733 1102 94-95 0.33398129868795934 1102 96-97 0.3666046532296363 1102 98-99 0.37014309390601596 1102 100 0.36617294561077074 1103 1 0.2751580649282914 1103 2 0.24748903853452475 1103 3 0.2404037842131217 1103 4 0.16488582850650602 1103 5 0.17257625284040046 1103 6 0.18222229823569336 1103 7 0.19310831007530993 1103 8 0.167866141788096 1103 9 0.23130917359166148 1103 10-11 0.25059600444125607 1103 12-13 0.25863513837713015 1103 14-15 0.3222390269398048 1103 16-17 0.3038078984565047 1103 18-19 0.3199308926054698 1103 20-21 0.33540327465724573 1103 22-23 0.3298822780574753 1103 24-25 0.32839161402966965 1103 26-27 0.29072126742200055 1103 28-29 0.3575533752094273 1103 30-31 0.36428132797741597 1103 32-33 0.31741637663377276 1103 34-35 0.3236230242483771 1103 36-37 0.3361903121181129 1103 38-39 0.3751797014672462 1103 40-41 0.3323372294187905 1103 42-43 0.32691078629328274 1103 44-45 0.3241384799799576 1103 46-47 0.3455801414407915 1103 48-49 0.35525439381293467 1103 50-51 0.3531803772231825 1103 52-53 0.3336178903602871 1103 54-55 0.22955690931070194 1103 56-57 0.24328274006099093 1103 58-59 0.27775880933304364 1103 60-61 0.23593325563922463 1103 62-63 0.21090536581920816 1103 64-65 0.19782567730144507 1103 66-67 0.18548776314942472 1103 68-69 0.20294169752544633 1103 70-71 0.190911569244129 1103 72-73 0.208576270050699 1103 74-75 0.22114028098233263 1103 76-77 0.15263257807902875 1103 78-79 0.335718221538265 1103 80-81 0.3084579112003851 1103 82-83 0.31818691247243436 1103 84-85 0.29477921356365755 1103 86-87 0.3087809837131843 1103 88-89 0.3256022553812912 1103 90-91 0.26962288242150834 1103 92-93 0.23844947973128683 1103 94-95 0.2505082973365518 1103 96-97 0.2910916318723551 1103 98-99 0.29010462416582783 1103 100 0.2896955492334925 1104 1 0.23627595192149897 1104 2 0.1941873126416951 1104 3 0.1927221310259597 1104 4 0.14084366655213643 1104 5 0.1535630942995212 1104 6 0.16273246623273963 1104 7 0.18067021889225288 1104 8 0.15969353336254244 1104 9 0.21578526289289357 1104 10-11 0.22275126503621578 1104 12-13 0.20027131199083925 1104 14-15 0.23457155551510311 1104 16-17 0.25030031532715213 1104 18-19 0.24867453840117548 1104 20-21 0.2652029315837794 1104 22-23 0.2796716486605817 1104 24-25 0.28797473364176085 1104 26-27 0.26582258819276916 1104 28-29 0.2783431203443598 1104 30-31 0.27458206914398176 1104 32-33 0.29436938864157725 1104 34-35 0.3096610434394762 1104 36-37 0.31665796298163684 1104 38-39 0.3115580375013849 1104 40-41 0.2977586969414645 1104 42-43 0.29738252517250885 1104 44-45 0.32362216478225747 1104 46-47 0.282649348077598 1104 48-49 0.31773054626290786 1104 50-51 0.2971507525937227 1104 52-53 0.3141796479962977 1104 54-55 0.1986254252619446 1104 56-57 0.21589511891198043 1104 58-59 0.19626977163000703 1104 60-61 0.12264244595697704 1104 62-63 0.09659900185456394 1104 64-65 0.1361387885697738 1104 66-67 0.128508502109284 1104 68-69 0.09964365564241007 1104 70-71 0.12329808664041053 1104 72-73 0.14953037052912066 1104 74-75 0.153527067165804 1104 76-77 0.14301848018016727 1104 78-79 0.2519527875775154 1104 80-81 0.21708460571237964 1104 82-83 0.23396555417087228 1104 84-85 0.22042381676384792 1104 86-87 0.23481370562124226 1104 88-89 0.2507129659514149 1104 90-91 0.2639272580110017 1104 92-93 0.2456269191953595 1104 94-95 0.24852450031871598 1104 96-97 0.27759889474739197 1104 98-99 0.26432498302909124 1104 100 0.26950305749648606 1105 1 0.19852607299476688 1105 2 0.15825690904458156 1105 3 0.13888369975674664 1105 4 0.14347480256829215 1105 5 0.10359923976572105 1105 6 0.11634896554381413 1105 7 0.1207190516087806 1105 8 0.13109056314690548 1105 9 0.15466518626872272 1105 10-11 0.1643238459119587 1105 12-13 0.14891839693135722 1105 14-15 0.19906401429471998 1105 16-17 0.1810662117522739 1105 18-19 0.21212247190355527 1105 20-21 0.19780312089734053 1105 22-23 0.2135561022516157 1105 24-25 0.20438220526582995 1105 26-27 0.17460336897639195 1105 28-29 0.21408475624036072 1105 30-31 0.21051174576826526 1105 32-33 0.21050375130469945 1105 34-35 0.22330596428553662 1105 36-37 0.19764903159980207 1105 38-39 0.18723116194227885 1105 40-41 0.1884311280116151 1105 42-43 0.20283225460807586 1105 44-45 0.21685105454383802 1105 46-47 0.1952326047390116 1105 48-49 0.20774472331338956 1105 50-51 0.1883331621164288 1105 52-53 0.20415456667510057 1105 54-55 0.09109248978415962 1105 56-57 0.0999462444130188 1105 58-59 0.0645801586546213 1105 60-61 0.07417644965043735 1105 62-63 0.031014262830503014 1105 64-65 0.08517831243019458 1105 66-67 0.0493898548750451 1105 68-69 0.03607484124440674 1105 70-71 0.0992841042798247 1105 72-73 0.1467999761751173 1105 74-75 0.15849777091907669 1105 76-77 0.08403615876206771 1105 78-79 0.21051171155954052 1105 80-81 0.23318805490726646 1105 82-83 0.25703100131712375 1105 84-85 0.27244972295904546 1105 86-87 0.27693448629895556 1105 88-89 0.2831626624651946 1105 90-91 0.2741347391834026 1105 92-93 0.22065189621676495 1105 94-95 0.23364495229347426 1105 96-97 0.23694223919456903 1105 98-99 0.2477416857969459 1105 100 0.25559253231072177 1106 1 0.22889257230673365 1106 2 0.19345505032892873 1106 3 0.1908731094781544 1106 4 0.12115364533066497 1106 5 0.13321568956499874 1106 6 0.15342628781585432 1106 7 0.16376888750741614 1106 8 0.16976909492011316 1106 9 0.19054080042917576 1106 10-11 0.20160450198292068 1106 12-13 0.18658404471295142 1106 14-15 0.2622139834064683 1106 16-17 0.25083949989035403 1106 18-19 0.25145912310358653 1106 20-21 0.2431944032841713 1106 22-23 0.24950944507530437 1106 24-25 0.25834529185900124 1106 26-27 0.26048308036205015 1106 28-29 0.26704359602770467 1106 30-31 0.268603468460725 1106 32-33 0.29685615302292945 1106 34-35 0.27770822900252057 1106 36-37 0.2615811659658007 1106 38-39 0.2822748577500178 1106 40-41 0.30603737760092287 1106 42-43 0.29959709205482454 1106 44-45 0.3161810610596163 1106 46-47 0.3194368458618726 1106 48-49 0.28291333765643856 1106 50-51 0.2768285863920923 1106 52-53 0.2772511211080797 1106 54-55 0.1641998692416351 1106 56-57 0.16753990680926023 1106 58-59 0.1821720403748941 1106 60-61 0.146461165703478 1106 62-63 0.12002928227286702 1106 64-65 0.1302867873717375 1106 66-67 0.12973291925583652 1106 68-69 0.15736116051152038 1106 70-71 0.11576701544970547 1106 72-73 0.129054953076718 1106 74-75 0.14376633729504107 1106 76-77 0.0918537859456805 1106 78-79 0.22705587019520834 1106 80-81 0.2485079197974045 1106 82-83 0.24036700308058556 1106 84-85 0.2692394479272764 1106 86-87 0.29566680450925986 1106 88-89 0.2985758460968597 1106 90-91 0.2808744931706286 1106 92-93 0.2571853479868693 1106 94-95 0.26349680416294774 1106 96-97 0.24349885227480428 1106 98-99 0.28802834030511093 1106 100 0.2915218695985544 1107 1 0.17112199853865917 1107 2 0.14777254471330536 1107 3 0.13911435825813356 1107 4 0.10611446352955056 1107 5 0.11771250891111151 1107 6 0.10485440572686144 1107 7 0.116577117014522 1107 8 0.1390111874390314 1107 9 0.17162538114200032 1107 10-11 0.16022879741878882 1107 12-13 0.1378044694381586 1107 14-15 0.17365348291659188 1107 16-17 0.16644692160287633 1107 18-19 0.1979096958464055 1107 20-21 0.1776182426591575 1107 22-23 0.1765875429141488 1107 24-25 0.1753740950938223 1107 26-27 0.17105544268312656 1107 28-29 0.18761263130885908 1107 30-31 0.1846246632960984 1107 32-33 0.1986607509178313 1107 34-35 0.20466032879465246 1107 36-37 0.18202340087621138 1107 38-39 0.1592052753188966 1107 40-41 0.18536523090386936 1107 42-43 0.14298695688120233 1107 44-45 0.13661701044318164 1107 46-47 0.15146012043906865 1107 48-49 0.18439709747604383 1107 50-51 0.1680959022018058 1107 52-53 0.18343540560201177 1107 54-55 0.08420562697934031 1107 56-57 0.0739769037134721 1107 58-59 0.06991174261878541 1107 60-61 0.012053600850933321 1107 62-63 0.02242900555192051 1107 64-65 0.051868225952063085 1107 66-67 0.0338297384707289 1107 68-69 -0.028524791845626396 1107 70-71 0.02174200210977517 1107 72-73 0.07173031665571727 1107 74-75 0.07380403584721762 1107 76-77 -0.02652638700916299 1107 78-79 0.15983962717302092 1107 80-81 0.15799892809979355 1107 82-83 0.16607654991565113 1107 84-85 0.20713149940178965 1107 86-87 0.18897789211265348 1107 88-89 0.21235252164606777 1107 90-91 0.1972882407026475 1107 92-93 0.1672984092896712 1107 94-95 0.18545088170839108 1107 96-97 0.14277970000539852 1107 98-99 0.18839948249973304 1107 100 0.2103482411165274 1108 1 0.04122765954804919 1108 2 0.036024188170138416 1108 3 0.016621073660530783 1108 4 0.018705128181117914 1108 5 0.02391231971576957 1108 6 0.031027037313386074 1108 7 0.029220525955388155 1108 8 0.027745592510122208 1108 9 0.0221258419655328 1108 10-11 0.03685799890730834 1108 12-13 0.02076394524241465 1108 14-15 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2316 64-65 -0.4215130596417893 2316 66-67 -0.48766651631778757 2316 68-69 -0.48035519034299057 2316 70-71 -0.48382627948059564 2316 72-73 -0.477692911110978 2316 74-75 -0.48506315346272544 2316 76-77 -0.5425673738132915 2316 78-79 -0.6996845622964116 2316 80-81 -0.6777967948859356 2316 82-83 -0.6514810074950717 2316 84-85 -0.6534610612576088 2316 86-87 -0.6582821211130891 2316 88-89 -0.7475074135834845 2316 90-91 -0.6706629283424519 2316 92-93 -0.5955929652720329 2316 94-95 -0.580081172117282 2316 96-97 -0.617289630846173 2316 98-99 -1.427522443489572 2316 100 -0.9785437302496938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 12.0 7 329.0 8 559.0 9 172.0 10 66.0 11 48.0 12 131.0 13 380.0 14 1532.0 15 4721.0 16 10982.0 17 18571.0 18 24321.0 19 31324.0 20 42044.0 21 57955.0 22 85726.0 23 138701.0 24 233395.0 25 319260.0 26 345980.0 27 319046.0 28 312278.0 29 346474.0 30 412931.0 31 494346.0 32 611981.0 33 795899.0 34 1135086.0 35 1898821.0 36 3866184.0 37 8736082.0 38 1.0594658E7 39 2342710.0 40 2325.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.66717146661005 29.13858577463262 28.441380249863602 24.752862508893728 2 23.2029683709068 31.11318794328658 25.392588529754605 20.291255156052017 3 24.804681508499467 24.936032903993155 26.811143458360593 23.448142129146788 4 21.27128406995564 27.44800893655965 24.04241008671681 27.2382969067679 5 23.64416425116988 28.856767644929054 25.96623537781946 21.53283272608161 6 27.545351021228548 27.33055838732103 24.717346948307718 20.406743643142704 7 23.186843585797572 25.350264863403773 28.763984844973773 22.698906705824886 8 26.08055801064516 26.78093706710526 24.867318185338387 22.27118673691119 9 25.017940318270014 25.17696382977505 26.867530329187588 22.937565522767343 10-11 22.63906345722755 27.984457148298496 26.957815014782266 22.418664379691684 12-13 22.444701722433276 26.463034687628728 27.040163893177134 24.05209969676086 14-15 22.39963170140271 29.414627619743 25.661081216440063 22.524659462414228 16-17 24.020431803135327 26.152854163458645 25.760608021146886 24.06610601225914 18-19 22.439249764343355 27.626854030411934 25.896274975152128 24.037621230092583 20-21 23.93399288217621 27.776547945865964 25.82746509644621 22.461994075511612 22-23 23.987116563095338 26.30170034131763 27.216371204647338 22.494811890939697 24-25 22.570755323576275 27.528760977044147 25.638774685292702 24.261709014086872 26-27 22.6220564588638 26.013071418762124 28.811013688757054 22.553858433617027 28-29 22.6455421205369 26.13037529535964 28.698739598957307 22.525342985146153 30-31 22.583209384612115 25.9971075032084 28.826308701855694 22.593374410323786 32-33 22.693992076944166 26.226063401865552 28.515317714068832 22.56462680712145 34-35 22.696843841430677 26.284147241693194 28.400097521034002 22.618911395842126 36-37 22.600126323654365 26.235588552321154 28.572735885977686 22.591549238046795 38-39 22.64365907863431 26.244532547905575 28.612789708600612 22.499018664859506 40-41 22.673859153871735 26.402085478304983 28.50932431061097 22.414731057212315 42-43 22.683768596476146 26.30660361825808 28.509221820649017 22.500405964616764 44-45 22.76099311932108 26.399261951739412 28.428584250011784 22.41116067892773 46-47 22.842444634481886 26.5153471377233 28.304870138398293 22.33733808939652 48-49 22.807870293836725 26.596892561740365 28.216500170029434 22.378736974393473 50-51 22.907184269156605 26.644397655043147 28.047052428212847 22.401365647587397 52-53 22.989443775919067 26.75012168109602 27.821263449214083 22.439171093770828 54-55 23.049992666972095 26.915794832272017 27.461697015155213 22.57251548560068 56-57 23.299975626133854 27.218634182657997 26.919503798763568 22.56188639244458 58-59 23.370174981123235 27.477543085953332 26.504957006916598 22.64732492600684 60-61 23.414775855707486 27.600062763271787 26.29208318037716 22.69307820064357 62-63 23.31280252381733 27.721804952151786 26.290896478534993 22.67449604549589 64-65 23.367429540727795 27.818488120633784 26.09286491659831 22.721217422040116 66-67 23.214888334430604 27.786875576390997 26.1590313695283 22.8392047196501 68-69 23.144336058917936 27.775756074844537 26.21207381967715 22.86783404656037 70-71 23.05593788855513 27.711035517022548 26.23474932779902 22.998277266623308 72-73 22.94674530635464 27.663040640821297 26.241234664241986 23.148979388582074 74-75 22.93424849420797 27.705295392184087 26.126553969462574 23.233902144145375 76-77 22.884574350739765 27.726757369614514 26.024589793386788 23.364078486258936 78-79 22.826265614974826 27.763908180587784 25.875308035147874 23.534518169289516 80-81 22.79886131924686 27.824132061050587 25.721503881301157 23.655502738401392 82-83 22.716448028945745 27.84829248473908 25.577731761119555 23.85752772519562 84-85 22.61752674477886 27.81267001673144 25.5410656759472 24.028737562542503 86-87 22.571898408109732 27.784210085320154 25.481323279456593 24.16256822711352 88-89 22.497203688336402 27.729205080559467 25.440774582285997 24.332816648818138 90-91 22.418484496681764 27.61169267441186 25.46077517721253 24.509047651693848 92-93 22.371997481817633 27.53848577899136 25.497999251645215 24.59151748754579 94-95 22.30578531648014 27.473244710695603 25.518913676627246 24.702056296197007 96-97 22.21759106536386 27.384886320794134 25.580574851875053 24.816947761966954 98-99 22.175998031853673 27.364110641392497 25.603158785656248 24.856732541097585 100 22.08327617800448 27.335508369830375 25.603310155390975 24.97790529677417 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37931.0 1 25548.0 2 10621.0 3 7390.5 4 6669.0 5 6449.5 6 6477.0 7 6785.5 8 6724.0 9 6920.5 10 7510.0 11 11053.0 12 93462.0 13 107326.5 14 40274.0 15 40903.0 16 45874.0 17 50963.5 18 56441.5 19 62493.0 20 70418.5 21 82860.5 22 100482.5 23 121187.0 24 142460.5 25 166351.5 26 192331.5 27 223632.5 28 267702.5 29 314268.0 30 352900.0 31 396080.0 32 442275.0 33 478797.0 34 515527.0 35 567537.5 36 626953.0 37 673192.0 38 725011.0 39 782856.0 40 831716.0 41 891637.5 42 963458.5 43 1010643.5 44 1040741.0 45 1088766.0 46 1141166.0 47 1183916.0 48 1222108.5 49 1260650.5 50 1297053.0 51 1328212.5 52 1342075.5 53 1329825.0 54 1295620.0 55 1238192.5 56 1161651.0 57 1066257.0 58 949299.0 59 815245.5 60 671095.5 61 537074.0 62 422156.0 63 319710.0 64 235514.5 65 172200.5 66 124305.5 67 90862.5 68 67501.5 69 51510.0 70 40072.5 71 31579.5 72 25898.5 73 20436.0 74 16148.0 75 13787.0 76 10438.0 77 6001.5 78 3378.5 79 2251.5 80 1384.5 81 795.5 82 438.0 83 233.5 84 135.0 85 83.5 86 49.5 87 28.0 88 19.5 89 13.0 90 7.0 91 3.5 92 2.0 93 2.0 94 0.5 95 0.5 96 1.5 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10626478264446348 2 0.07378025573579412 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.923004740390471E-4 20-21 0.0021997870726649937 22-23 7.578718476373232E-4 24-25 8.000595449213093E-4 26-27 0.006783179041875207 28-29 0.006566213741557563 30-31 0.00791019324074741 32-33 0.007928273682440546 34-35 0.007367779989953301 36-37 0.010836211388086736 38-39 0.013468422357912587 40-41 0.01713724531814496 42-43 0.023738113239614368 44-45 0.012059654609322335 46-47 0.01864846890299632 48-49 0.01651648348668059 50-51 0.019100479945324742 52-53 0.021441897144585977 54-55 0.11167234141418586 56-57 0.10477615961172855 58-59 0.1019299967485339 60-61 0.14408755996303152 62-63 0.09474754128593525 64-65 0.07353164966251349 66-67 0.08359642887169305 68-69 0.07658875101212806 70-71 0.05327854155925127 72-73 0.03194814047177296 74-75 0.020769907394991054 76-77 0.012626175115707294 78-79 0.008859416429637099 80-81 0.005702872650710275 82-83 5.650138029105292E-4 84-85 0.0023986719312895 86-87 0.0016588805253453139 88-89 0.0020867843120828877 90-91 0.0030284739836004366 92-93 0.004735569020127449 94-95 0.0019798083653984944 96-97 0.0011360544197187709 98-99 0.0015232772126467869 100 0.0018261246110068306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.318503E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.48168365414914 #Duplication Level Percentage of deduplicated Percentage of total 1 74.23444630020406 33.02073156566599 2 11.949177004377587 10.630390228723146 3 4.7234292390028445 6.303182555162487 4 2.559405186866158 4.553866074598756 5 1.5037149506879905 3.3443886371258826 6 1.0183285072261725 2.7178179908661915 7 0.7075755463981113 2.2031906131404755 8 0.525476432920283 1.8699261165496597 9 0.38947499745092184 1.5592053265031203 >10 2.1065993551354545 16.78174425717039 >50 0.17639582722907657 5.536408679899886 >100 0.10449213910869704 7.8876830528450155 >500 0.0011209552394227734 0.33580399482973106 >1k 3.229568296435332E-4 0.24069606274413047 >5k 6.766887279545175E-6 0.021139408334478162 >10k+ 3.3834436393037715E-5 2.9938254358406167 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 882086 2.658084081888731 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG 33371 0.10056040329027877 TruSeq Adapter, Index 16 (97% over 38bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.11058902161607206 1.2053627795424624E-5 0.0 0.0 3.013406948856156E-6 2 0.11059203502302092 3.3147476437417716E-5 0.0 0.0 6.026813897712312E-6 3 0.11059203502302092 3.917429033513003E-5 0.0 0.0 9.040220846568468E-6 4 0.11059203502302092 4.218769728398618E-5 0.0 0.0 1.506703474428078E-5 5 0.11059504842996978 5.122791813055465E-5 0.0 0.0 2.7120662539705405E-5 6 0.11059504842996978 5.122791813055465E-5 0.0 0.0 3.013406948856156E-5 7 0.11059806183691863 6.026813897712312E-5 0.0 0.0 3.013406948856156E-5 8 0.11060710205776521 6.629495287483543E-5 0.0 0.0 3.917429033513003E-5 9 0.11061312887166291 7.53351737214039E-5 0.0 3.013406948856156E-6 4.218769728398618E-5 10-11 0.11062819590640718 8.136198761911622E-5 0.0 6.026813897712312E-6 4.520110423284234E-5 12-13 0.11065079645852362 8.588209804240044E-5 0.0 9.040220846568468E-6 4.8214511181698495E-5 14-15 0.1106764104175889 9.040220846568468E-5 0.0 1.2053627795424624E-5 5.424132507941081E-5 16-17 0.11071257130097517 9.341561541454083E-5 0.0 1.506703474428078E-5 6.328154592597927E-5 18-19 0.11073969196351488 1.0546924320996545E-4 0.0 1.9587145167565015E-5 6.478824940040735E-5 20-21 0.11078489306774772 1.2053627795424623E-4 0.0 2.109384864199309E-5 8.286869109354428E-5 22-23 0.11098528462984665 1.3258990574967086E-4 0.0 2.109384864199309E-5 9.341561541454083E-5 24-25 0.1120339502480486 1.506703474428078E-4 0.0 2.109384864199309E-5 9.944242931225315E-5 26-27 0.11636873614397818 1.6724408566151666E-4 0.0 2.4107255590849248E-5 1.0697594668439354E-4 28-29 0.13063269793638876 1.777910099825132E-4 0.0 2.4107255590849248E-5 1.1149605710767776E-4 30-31 0.16166928280613277 2.0039156209893436E-4 0.0 2.4107255590849248E-5 1.1752287100539008E-4 32-33 0.2184072155426709 2.1545859684321517E-4 0.0 2.4107255590849248E-5 1.235496849031024E-4 34-35 0.31307640824793587 2.3052563158749593E-4 0.0 2.5613959065277326E-5 1.295764988008147E-4 36-37 0.4541008400474551 2.486060732806329E-4 0.0 2.7120662539705405E-5 1.431368300706674E-4 38-39 0.6484007999992768 2.5915299760162936E-4 0.0 2.862736601413348E-5 1.5368375439166394E-4 40-41 0.894787499062077 2.6969992192262595E-4 0.0 3.3147476437417716E-5 1.6875078913594472E-4 42-43 1.1871166004671383 2.877803636157629E-4 0.0 3.4654179911845794E-5 1.777910099825132E-4 44-45 1.5264156759840204 2.9832728793675943E-4 0.0 3.616088338627387E-5 1.9285804472679398E-4 46-47 1.9234184811645492 3.1640772962989637E-4 0.0 4.068099380955811E-5 2.0189826557336245E-4 48-49 2.379841151266098 3.3147476437417713E-4 0.0 4.520110423284234E-5 2.0792507947107477E-4 50-51 2.8862426823179006 3.5106190954174217E-4 0.0 4.8214511181698495E-5 2.1395189336878708E-4 52-53 3.4352974820272877 3.585954269138826E-4 0.0 4.9721214656126574E-5 2.2600552116421168E-4 54-55 4.024251296443005 3.887294964024441E-4 0.0 6.026813897712312E-5 2.3353903853635206E-4 56-57 4.653054404350396 4.083166415700091E-4 0.0 6.026813897712312E-5 2.4408596285734863E-4 58-59 5.327617603479641 4.399574145329988E-4 0.0 6.026813897712312E-5 2.531261837039171E-4 60-61 6.042775914320403 4.520110423284234E-4 0.0 6.478824940040735E-5 2.6367310802491363E-4 62-63 6.786621859314275 4.6105126317499186E-4 0.0 6.629495287483543E-5 2.7422003234591017E-4 64-65 7.5562158599826486 4.746115944448446E-4 0.0 6.629495287483543E-5 2.832602531924786E-4 66-67 8.351966232967094 4.851585187658411E-4 0.0 6.780165634926351E-5 2.938071775134752E-4 68-69 9.176577511004208 4.987188500356938E-4 0.0 6.930835982369159E-5 3.0887421225775597E-4 70-71 10.026328136512156 5.167992917288307E-4 0.0 7.081506329811967E-5 3.1339432268104024E-4 72-73 10.89448917177414 5.348797334219677E-4 0.0 7.232176677254775E-5 3.224345435276087E-4 74-75 11.78040369407531 5.499467681662484E-4 0.0 7.232176677254775E-5 3.254479504764648E-4 76-77 12.683202335510922 5.635070994361012E-4 0.0 7.684187719583198E-5 3.2846135742532095E-4 78-79 13.600296579511905 5.78574134180382E-4 0.0 7.834858067026006E-5 3.3147476437417713E-4 80-81 14.532139943824067 5.99667982822375E-4 0.0 7.834858067026006E-5 3.4051498522074563E-4 82-83 15.48043500337351 6.207618314643681E-4 0.0 7.834858067026006E-5 3.4654179911845794E-4 84-85 16.435864303874368 6.298020523109366E-4 0.0 7.834858067026006E-5 3.631155373371668E-4 86-87 17.400629139102783 6.478824940040736E-4 0.0 7.834858067026006E-5 3.7667586860701946E-4 88 18.12852964122678 6.539093079017858E-4 0.0 7.834858067026006E-5 3.8571608945358796E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 122265 0.0 78.12586 8 GTGGTAT 123440 0.0 77.46212 6 AGTGGTA 124450 0.0 76.89386 5 TGGTATC 124275 0.0 76.72619 7 GTATCAA 124530 0.0 76.50872 9 AAGCAGT 132495 0.0 72.96185 1 GCAGTGG 137890 0.0 69.93057 3 CAGTGGT 137680 0.0 69.84275 4 AGCAGTG 146830 0.0 65.90698 2 CAACGCA 118785 0.0 42.554764 12-13 ATCAACG 120725 0.0 41.901867 10-11 ACGCAGA 123550 0.0 41.004005 14-15 AGAGTAC 124600 0.0 40.29471 18-19 TCAACGC 118340 0.0 40.14237 12-13 GAGTACT 116935 0.0 39.130295 20-21 AACGCAG 121270 0.0 39.12513 14-15 AGTACTT 126340 0.0 38.545444 20-21 CGCAGAG 126340 0.0 37.550514 16-17 TATCAAC 126930 0.0 37.54585 10-11 GTACTTT 122850 0.0 37.180973 22-23 >>END_MODULE