FastQCFastQC Report
Thu 26 May 2016
SRR1660653_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660653_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27092030
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7530582.7796292858084093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1059300.078.633728
GTGGTAT1067300.078.103846
AGTGGTA1073200.077.7860645
TGGTATC1077500.077.206837
GTATCAA1083800.076.595899
AAGCAGT1128750.074.978151
CAGTGGT1163300.072.073294
GCAGTGG1171700.071.9177863
AGCAGTG1260150.067.292542
CAACGCA1044400.041.99989312-13
ATCAACG1059000.041.48102610-11
ACGCAGA1077100.040.6856214-15
AGAGTAC1074400.040.22696718-19
TCAACGC1040250.039.84137312-13
GAGTACT1000500.039.24146720-21
AGTACTT1070950.038.91305520-21
AACGCAG1063650.038.83892414-15
TATCAAC1106700.037.52146510-11
CGCAGAG1098900.037.4740716-17
GTACTTT1053750.037.16552722-23
Produced by FastQC (version 0.11.5)