##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660653_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27092030 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41250729458073 34.0 31.0 34.0 31.0 34.0 2 32.56980414535197 34.0 31.0 34.0 31.0 34.0 3 32.59112720604547 34.0 31.0 34.0 31.0 34.0 4 35.9478680630429 37.0 37.0 37.0 35.0 37.0 5 35.94510710345441 37.0 37.0 37.0 35.0 37.0 6 35.95957309216031 37.0 37.0 37.0 35.0 37.0 7 35.93226782932102 37.0 37.0 37.0 35.0 37.0 8 35.94163552897291 37.0 37.0 37.0 35.0 37.0 9 37.6774466143733 39.0 38.0 39.0 35.0 39.0 10-11 37.62784638138966 39.0 38.0 39.0 35.0 39.0 12-13 37.65968744313365 39.0 38.0 39.0 35.0 39.0 14-15 39.143790959924374 41.0 39.0 41.0 36.0 41.0 16-17 39.10433470655392 41.0 39.0 41.0 36.0 41.0 18-19 39.076194050427375 41.0 39.0 41.0 36.0 41.0 20-21 39.01951280505743 41.0 39.0 41.0 36.0 41.0 22-23 38.90619911095625 40.5 39.0 41.0 35.5 41.0 24-25 38.88184087718787 40.5 39.0 41.0 35.0 41.0 26-27 38.82626241001505 40.0 39.0 41.0 35.0 41.0 28-29 38.73559404740065 40.0 39.0 41.0 35.0 41.0 30-31 38.63820429845973 40.0 38.0 41.0 34.5 41.0 32-33 38.528709513462076 40.0 38.0 41.0 34.0 41.0 34-35 38.42122498757014 40.0 38.0 41.0 34.0 41.0 36-37 38.2762732065482 40.0 38.0 41.0 34.0 41.0 38-39 37.989964502475445 40.0 38.0 41.0 33.0 41.0 40-41 37.845677068126676 40.0 37.0 41.0 33.0 41.0 42-43 37.694412120464946 40.0 37.0 41.0 33.0 41.0 44-45 37.5461252626695 40.0 37.0 41.0 32.5 41.0 46-47 37.36947668742431 40.0 36.0 41.0 32.0 41.0 48-49 37.38183100343532 40.0 36.0 41.0 32.0 41.0 50-51 36.41724656661018 38.5 35.5 40.0 31.0 40.5 52-53 36.63777260323424 39.0 35.5 39.5 31.5 40.5 54-55 37.14038820642085 40.0 36.0 41.0 31.5 41.0 56-57 36.931349256589485 40.0 36.0 41.0 31.0 41.0 58-59 36.70961371296282 39.0 35.0 41.0 31.0 41.0 60-61 36.46796690761084 39.0 35.0 41.0 30.0 41.0 62-63 36.182381663537214 39.0 35.0 41.0 30.0 41.0 64-65 35.876880008622464 37.5 35.0 40.5 29.5 41.0 66-67 35.558560783374304 37.0 35.0 40.0 29.0 41.0 68-69 35.240870064000376 36.5 35.0 39.5 29.0 41.0 70-71 34.89351999462573 36.0 35.0 39.0 29.0 41.0 72-73 34.53613922618571 35.5 34.0 39.0 29.0 41.0 74-75 34.18322392231221 35.0 34.0 37.5 28.0 40.0 76-77 33.83645180888992 35.0 34.0 37.0 28.0 39.0 78-79 33.509105500769046 35.0 34.0 36.5 27.0 39.0 80-81 33.20583882049444 35.0 34.0 36.0 27.0 38.0 82-83 32.949814724108904 35.0 34.0 36.0 26.0 37.0 84-85 32.723265200134506 35.0 33.0 35.0 26.0 37.0 86-87 32.530429188953356 35.0 33.0 35.0 26.0 36.5 88-89 32.352587310733085 35.0 33.0 35.0 25.0 36.0 90-91 32.22873644020031 35.0 33.0 35.0 25.0 36.0 92-93 32.10755194424338 35.0 33.0 35.0 25.0 36.0 94-95 32.00588566083826 35.0 33.0 35.0 25.0 35.0 96-97 31.90924240450051 35.0 33.0 35.0 24.0 35.0 98-99 31.668166135944777 35.0 33.0 35.0 23.5 35.0 100 31.601618003523544 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48969432049700146 1101 2 0.474629880203274 1101 3 0.47244567906351875 1101 4 0.42427282790638543 1101 5 0.39333732292331547 1101 6 0.39070420901288117 1101 7 0.39505177481510145 1101 8 0.41903220140854813 1101 9 0.536010920754947 1101 10-11 0.5144122995954561 1101 12-13 0.4901954202535137 1101 14-15 0.6265590877953429 1101 16-17 0.6525779323810852 1101 18-19 0.6128334291607374 1101 20-21 0.6249569550404743 1101 22-23 0.6304702567546272 1101 24-25 0.6469396501330209 1101 26-27 0.6169248174723378 1101 28-29 0.6444015438850954 1101 30-31 0.6337800563219389 1101 32-33 0.6530250770112929 1101 34-35 0.6309734531571394 1101 36-37 0.6306098131581095 1101 38-39 0.6800476591873448 1101 40-41 0.6915738061407737 1101 42-43 0.6987744354657508 1101 44-45 0.69957689796464 1101 46-47 0.6873621081661625 1101 48-49 0.6759460975083229 1101 50-51 0.6275873712399331 1101 52-53 0.636651579377812 1101 54-55 0.7175271600085225 1101 56-57 0.6910691215223821 1101 58-59 0.6272949036382229 1101 60-61 0.6215385916884841 1101 62-63 0.6381551839748951 1101 64-65 0.6563424312027166 1101 66-67 0.6257416220711036 1101 68-69 0.5817981320667016 1101 70-71 0.5665579363487296 1101 72-73 0.5460169939763873 1101 74-75 0.5529637632104709 1101 76-77 0.5016664121646031 1101 78-79 0.5049113673495285 1101 80-81 0.5356119523894947 1101 82-83 0.5527965770923728 1101 84-85 0.580273873921918 1101 86-87 0.5495884027126365 1101 88-89 0.5427778565309609 1101 90-91 0.5344561503969061 1101 92-93 0.5399934441639758 1101 94-95 0.5063452307334444 1101 96-97 0.5058724981638356 1101 98-99 0.3493137798051187 1101 100 0.417260081084212 1102 1 0.47009582284522367 1102 2 0.438400236775891 1102 3 0.42875879886967994 1102 4 0.3942124468856605 1102 5 0.3942243047661833 1102 6 0.34398238464160613 1102 7 0.3658316411010816 1102 8 0.3734327491259819 1102 9 0.4426715472424476 1102 10-11 0.4590574587075693 1102 12-13 0.46688833945579233 1102 14-15 0.5463684675357143 1102 16-17 0.5828302376966192 1102 18-19 0.5637760607898343 1102 20-21 0.53661669158911 1102 22-23 0.5768601084379057 1102 24-25 0.5799775490661006 1102 26-27 0.5647616021181179 1102 28-29 0.5694451429587417 1102 30-31 0.6005129315699591 1102 32-33 0.5988131442170257 1102 34-35 0.5913438116104643 1102 36-37 0.6059522164281006 1102 38-39 0.6437632916007843 1102 40-41 0.6298353629684357 1102 42-43 0.6495911863471093 1102 44-45 0.6186305343704035 1102 46-47 0.6461994537396762 1102 48-49 0.6267366634539258 1102 50-51 0.6143189311231936 1102 52-53 0.6631055904982546 1102 54-55 0.6958665522148308 1102 56-57 0.6432146525281226 1102 58-59 0.6534955591906382 1102 60-61 0.638069259910786 1102 62-63 0.6265586483805734 1102 64-65 0.5933780352151601 1102 66-67 0.5472111632195293 1102 68-69 0.5511090780654797 1102 70-71 0.565935680444916 1102 72-73 0.5593128902082753 1102 74-75 0.5490593176061296 1102 76-77 0.5343140907497315 1102 78-79 0.5175661484511878 1102 80-81 0.4875768818871933 1102 82-83 0.45262477187376504 1102 84-85 0.4962733794988523 1102 86-87 0.46181340848789176 1102 88-89 0.5029163040538549 1102 90-91 0.47572106112147594 1102 92-93 0.4811120333044059 1102 94-95 0.5160520505428607 1102 96-97 0.4819138960125109 1102 98-99 0.3374774376929004 1102 100 0.38714380442527485 1103 1 0.39267814063838813 1103 2 0.3856858831469907 1103 3 0.3809848243561049 1103 4 0.3440125411425612 1103 5 0.3803364630617665 1103 6 0.330896426592723 1103 7 0.3477318090875414 1103 8 0.34770463930207995 1103 9 0.4457307621100881 1103 10-11 0.44459706091381435 1103 12-13 0.3812182517729923 1103 14-15 0.4944075246455455 1103 16-17 0.5341827088724003 1103 18-19 0.5270766519646486 1103 20-21 0.5057523200168177 1103 22-23 0.5079053902501727 1103 24-25 0.5256942895892962 1103 26-27 0.5024789710206292 1103 28-29 0.5285756982323377 1103 30-31 0.5234551653513577 1103 32-33 0.5252280034524404 1103 34-35 0.5261723342663984 1103 36-37 0.5258769817998612 1103 38-39 0.5530573593253365 1103 40-41 0.5639608317050246 1103 42-43 0.5613984151387399 1103 44-45 0.5578215968894042 1103 46-47 0.5766653335870444 1103 48-49 0.520636743319038 1103 50-51 0.4439314319184504 1103 52-53 0.517469001280233 1103 54-55 0.5938305785285252 1103 56-57 0.5878137261421799 1103 58-59 0.5852503640173055 1103 60-61 0.5439261998476397 1103 62-63 0.5202443689547849 1103 64-65 0.5163381712185142 1103 66-67 0.5329925349847571 1103 68-69 0.5072734585528593 1103 70-71 0.4821732860644232 1103 72-73 0.45830250074281054 1103 74-75 0.48916445172455525 1103 76-77 0.4731127826830672 1103 78-79 0.4785651789104932 1103 80-81 0.4654787250027894 1103 82-83 0.43907861245113367 1103 84-85 0.453767879635663 1103 86-87 0.4407336419929848 1103 88-89 0.45284795030265457 1103 90-91 0.44326280294693277 1103 92-93 0.4374713719742971 1103 94-95 0.46420777166680693 1103 96-97 0.43695109742681026 1103 98-99 0.33029669484230695 1103 100 0.392946755625605 1104 1 0.34603624970775826 1104 2 0.33019599502616614 1104 3 0.3306760952027332 1104 4 0.28231873268630636 1104 5 0.2880167171651635 1104 6 0.26231868811333214 1104 7 0.27585072098507624 1104 8 0.29333226521788447 1104 9 0.3532845694233657 1104 10-11 0.36562020386220695 1104 12-13 0.33197374181258965 1104 14-15 0.42423972112458586 1104 16-17 0.4303168178892278 1104 18-19 0.4262198543401112 1104 20-21 0.44284347729513485 1104 22-23 0.41951899918406355 1104 24-25 0.41054575859232756 1104 26-27 0.4173324546718149 1104 28-29 0.4362133434016542 1104 30-31 0.4652171776051901 1104 32-33 0.4433201523085657 1104 34-35 0.4404415725934214 1104 36-37 0.44513598318751946 1104 38-39 0.4209319577950268 1104 40-41 0.42561210300666374 1104 42-43 0.42979252458911077 1104 44-45 0.40197297400454346 1104 46-47 0.3915788431498939 1104 48-49 0.4051087071038779 1104 50-51 0.32131631282363315 1104 52-53 0.38888931247162617 1104 54-55 0.4580366158797986 1104 56-57 0.4243118310756202 1104 58-59 0.4208065122380944 1104 60-61 0.3990747334445004 1104 62-63 0.4031829650016263 1104 64-65 0.40938848536033134 1104 66-67 0.389520873468733 1104 68-69 0.4028617713614935 1104 70-71 0.4013653403792574 1104 72-73 0.3609497338218901 1104 74-75 0.35018824046209573 1104 76-77 0.3864873690406796 1104 78-79 0.38463054470984304 1104 80-81 0.41242003956612194 1104 82-83 0.38669186386675136 1104 84-85 0.3908837452539231 1104 86-87 0.34803566632965754 1104 88-89 0.3439512500825188 1104 90-91 0.34525100063862624 1104 92-93 0.3616951436023328 1104 94-95 0.3623000510070753 1104 96-97 0.3821296316017815 1104 98-99 0.25239610312363325 1104 100 0.3024259294792486 1105 1 0.342420761101387 1105 2 0.3305483006726391 1105 3 0.3586799366704696 1105 4 0.315283876466232 1105 5 0.30777517206200145 1105 6 0.2813815275838394 1105 7 0.2833638881397178 1105 8 0.2987609027144771 1105 9 0.36186478873408134 1105 10-11 0.3412040834374679 1105 12-13 0.3571944355518184 1105 14-15 0.45475168225394924 1105 16-17 0.4421267299911449 1105 18-19 0.4433714401600568 1105 20-21 0.432558305117432 1105 22-23 0.44097780214665505 1105 24-25 0.4479931131658219 1105 26-27 0.44497970371011064 1105 28-29 0.4289645252773724 1105 30-31 0.44206618818414256 1105 32-33 0.43970680278448526 1105 34-35 0.42420473402976455 1105 36-37 0.4619405369486387 1105 38-39 0.48127471592952276 1105 40-41 0.48729300879663384 1105 42-43 0.45209786462757506 1105 44-45 0.48144889587894824 1105 46-47 0.44755611526439765 1105 48-49 0.4538439135960033 1105 50-51 0.40027551274024376 1105 52-53 0.4625265066786497 1105 54-55 0.5125465525237658 1105 56-57 0.49484680524858504 1105 58-59 0.5069923767293361 1105 60-61 0.4649575871562277 1105 62-63 0.46588537824876397 1105 64-65 0.4866426100289445 1105 66-67 0.44960886793678156 1105 68-69 0.3993714931871253 1105 70-71 0.388575202867564 1105 72-73 0.3844307314127846 1105 74-75 0.3903627224224806 1105 76-77 0.3795767905024192 1105 78-79 0.3947905814354584 1105 80-81 0.3698707970558459 1105 82-83 0.37403135026023193 1105 84-85 0.3863674358339395 1105 86-87 0.3689925114838246 1105 88-89 0.3747452779109324 1105 90-91 0.3916920758490079 1105 92-93 0.404682093886926 1105 94-95 0.3865364997234124 1105 96-97 0.3742440107483951 1105 98-99 0.2827091821722192 1105 100 0.24476705427876766 1106 1 0.30010778533383586 1106 2 0.3075703830322922 1106 3 0.3289772640761939 1106 4 0.2792933021519417 1106 5 0.26688547295794507 1106 6 0.2668797035152721 1106 7 0.2895126992544732 1106 8 0.3028346040348424 1106 9 0.35106799527174104 1106 10-11 0.3680356473099451 1106 12-13 0.34466211825925086 1106 14-15 0.41196596181789147 1106 16-17 0.4442872956682038 1106 18-19 0.44442693173751735 1106 20-21 0.4413511408704309 1106 22-23 0.47171815977123543 1106 24-25 0.43680575409365474 1106 26-27 0.41583320556679126 1106 28-29 0.44860804767410656 1106 30-31 0.4870598011788374 1106 32-33 0.4899023711592747 1106 34-35 0.49248183889485375 1106 36-37 0.4889134488743352 1106 38-39 0.467002781035994 1106 40-41 0.47644359456551655 1106 42-43 0.4868942236622189 1106 44-45 0.459039314713074 1106 46-47 0.5006874323683519 1106 48-49 0.4460999760095774 1106 50-51 0.4351521422146334 1106 52-53 0.42182165804189253 1106 54-55 0.45916851931075797 1106 56-57 0.42398641898893885 1106 58-59 0.40450395152721086 1106 60-61 0.4202412263662296 1106 62-63 0.4394243358724381 1106 64-65 0.41148583397262684 1106 66-67 0.400618881607258 1106 68-69 0.4116994237793321 1106 70-71 0.4067880852356893 1106 72-73 0.4023167971281296 1106 74-75 0.4062178133490093 1106 76-77 0.4076287227947333 1106 78-79 0.38455115711830246 1106 80-81 0.3929833482424314 1106 82-83 0.40037970820812063 1106 84-85 0.41390283366639835 1106 86-87 0.41211060091867324 1106 88-89 0.41978908433041795 1106 90-91 0.392351740462729 1106 92-93 0.37675040458782405 1106 94-95 0.40676733091756034 1106 96-97 0.35946286680461625 1106 98-99 0.29878656575351314 1106 100 0.37615414092450195 1107 1 0.3205317526207878 1107 2 0.27052218736568534 1107 3 0.2503569986738441 1107 4 0.2487394215000336 1107 5 0.29173784110901835 1107 6 0.21475426927894148 1107 7 0.1990606335177958 1107 8 0.25079990298924315 1107 9 0.2850774498624773 1107 10-11 0.32491137448803187 1107 12-13 0.28416928692079324 1107 14-15 0.3593974619562488 1107 16-17 0.3826589905804738 1107 18-19 0.36509947986714764 1107 20-21 0.3608883224742101 1107 22-23 0.35775622955459596 1107 24-25 0.3969035189218033 1107 26-27 0.3800180421649699 1107 28-29 0.36124230802409585 1107 30-31 0.3970583732587727 1107 32-33 0.38035236637489334 1107 34-35 0.39020349298089485 1107 36-37 0.3927183936631451 1107 38-39 0.38928062632754035 1107 40-41 0.40793482228541933 1107 42-43 0.4032105988364947 1107 44-45 0.4489201606629152 1107 46-47 0.40720759192269895 1107 48-49 0.39344734274308024 1107 50-51 0.2969441145974301 1107 52-53 0.35654079851536125 1107 54-55 0.4614702436259037 1107 56-57 0.4379093309756712 1107 58-59 0.4852513223721715 1107 60-61 0.45183991412913826 1107 62-63 0.4515020290694238 1107 64-65 0.4320027269842228 1107 66-67 0.4351765288327698 1107 68-69 0.46311418124071935 1107 70-71 0.391321603496138 1107 72-73 0.409424718453657 1107 74-75 0.40004282814214775 1107 76-77 0.41659504399496683 1107 78-79 0.40279028470130385 1107 80-81 0.37304932539601054 1107 82-83 0.35541802911610887 1107 84-85 0.4177046615815456 1107 86-87 0.43044350092151973 1107 88-89 0.42469184526672166 1107 90-91 0.4205389009250098 1107 92-93 0.4274880794784579 1107 94-95 0.4406197179991054 1107 96-97 0.42537155638218493 1107 98-99 0.28914279224662565 1107 100 0.30868965073041466 1108 1 0.13761231351952574 1108 2 0.15077751878807533 1108 3 0.15570546066818736 1108 4 0.18227236551830117 1108 5 0.15498899755648665 1108 6 0.13992592871647247 1108 7 0.14020302555548625 1108 8 0.17620084951876436 1108 9 0.18443072078583356 1108 10-11 0.18681774788027639 1108 12-13 0.16952692488664667 1108 14-15 0.17721828109429083 1108 16-17 0.2274927037323735 1108 18-19 0.21184841105058894 1108 20-21 0.1948160490381028 1108 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-0.6520833083702762 2316 94-95 -0.6320352018052233 2316 96-97 -0.6593636105900309 2316 98-99 -0.5013879368677685 2316 100 -0.5365223670443591 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 749.0 3 246.0 4 222.0 5 237.0 6 704.0 7 5792.0 8 12145.0 9 15887.0 10 17309.0 11 17157.0 12 17461.0 13 19746.0 14 23829.0 15 32607.0 16 44430.0 17 57742.0 18 63694.0 19 70892.0 20 81586.0 21 98166.0 22 127139.0 23 182888.0 24 248578.0 25 303716.0 26 317260.0 27 294286.0 28 279062.0 29 294893.0 30 342750.0 31 420292.0 32 528857.0 33 696570.0 34 993012.0 35 1605319.0 36 3121037.0 37 6637981.0 38 7982099.0 39 2131581.0 40 4109.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.92553464566443 30.46858145317351 27.714842438326265 23.891041462835794 2 23.074140563996707 30.673938720878326 25.69916098613599 20.552759728988978 3 25.216363379280228 24.82270692252798 26.38610599079192 23.57482370739988 4 21.586871944931538 26.911575376877277 24.289079065150574 27.21247361304061 5 23.459030902553327 28.895365531276706 25.82797263054018 21.817630935629786 6 27.11063224398985 26.545771147880792 25.129617723760873 21.21397888436849 7 22.952495083041292 25.37904037849808 28.92708321677725 22.741381321683377 8 26.170297047545265 26.281881485635438 25.068714145278232 22.47910732154106 9 25.399515168110476 25.17115221457197 26.50395621610768 22.92537640120987 10-11 22.505372253163866 27.894449647021602 27.127867286102546 22.47231081371199 12-13 22.40526519948232 26.265177240039684 27.16090608581034 24.16865147466766 14-15 22.405334001258637 29.46943960261152 25.62974462839564 22.495481767734205 16-17 24.15239685427764 26.024868394625134 25.72171765704085 24.101017094056377 18-19 22.436388351400566 27.699240707999657 25.755182658116183 24.109188282483597 20-21 24.106350883463477 27.785605028914556 25.724327777698708 22.38371630992326 22-23 24.05539660700766 26.189708422248685 27.328603917970177 22.426291052773472 24-25 22.534570907968554 27.55548245104398 25.64787924814836 24.262067392839107 26-27 22.57581049536711 26.001678014200476 28.951173567041995 22.471337923390422 28-29 22.635817528154316 26.073264799641294 28.88222003724196 22.408697634962426 30-31 22.5499553188278 26.006956245602414 28.91045626552819 22.53263217004159 32-33 22.64431307430509 26.139586143269216 28.734113273985617 22.481987508440074 34-35 22.640052136924137 26.262343902033997 28.641965022641575 22.45563893840029 36-37 22.602822883547933 26.211087621534563 28.705586520206882 22.48050297471062 38-39 22.65491918914555 26.210861482095076 28.72276417223632 22.41145515652306 40-41 22.673601382926297 26.271194966825856 28.67875191278535 22.3764517374625 42-43 22.722425030067352 26.299270537871017 28.593601076609676 22.38470335545195 44-45 22.83612965880618 26.33986077974184 28.533754647240915 22.29025491421106 46-47 22.86580774365772 26.44582770241769 28.431406429476496 22.2569581244481 48-49 22.942513214913145 26.434112672732002 28.350470522698412 22.27290358965644 50-51 23.024826403576686 26.486356409061678 28.27740774808825 22.211409439273385 52-53 23.17292000367191 26.58090682023956 28.07250097736993 22.1736721987186 54-55 23.440590982406313 26.719763284501386 27.610771080932672 22.22887465215963 56-57 23.776478426708707 27.011197364604893 26.998479338330327 22.21384487035607 58-59 23.989572909975305 27.28242766331823 26.617866075779528 22.110133350926937 60-61 24.178768439989454 27.385549924976825 26.415997101932014 22.019684533101703 62-63 24.325303471332106 27.51587624952947 26.292457509952023 21.866362769186406 64-65 24.50077025686952 27.585159560725124 26.200759336935715 21.713310845469643 66-67 24.64893602508517 27.458812023791268 26.261953197180326 21.630298753943233 68-69 24.790857902109458 27.399003953916985 26.347676947781217 21.46246119619234 70-71 24.984547607354457 27.31521280324911 26.397143411588843 21.303096177807593 72-73 25.176556582593335 27.253804495530463 26.36782562907295 21.20181329280325 74-75 25.42932276606247 27.286816229432098 26.271515712081257 21.01234529242418 76-77 25.677168769049192 27.34583711864598 26.051935954319383 20.92505815798544 78-79 25.930333444341503 27.363655191912617 25.85207323910107 20.85393812464481 80-81 26.16477673898273 27.425161553560084 25.65303623474573 20.75702547271146 82-83 26.323003579206116 27.46863089882774 25.510009363227425 20.69835615873872 84-85 26.49625733730084 27.418527412744904 25.41842254470785 20.66679270524641 86-87 26.646293128412644 27.38593841837278 25.3588775223562 20.60889093085838 88-89 26.816313969288675 27.37097176230701 25.28600953107551 20.526704737328807 90-91 26.92907266840066 27.316426377288554 25.239011578663135 20.51548937564765 92-93 27.00407078732073 27.3748017864224 25.210575646882287 20.41055177937458 94-95 27.08570468133126 27.429281598004764 25.15823376641023 20.326779954253745 96-97 27.128049483277174 27.450037424980422 25.11810935383029 20.303803737912112 98-99 27.19187634477948 27.55935830072952 25.054434520165795 20.194330834325207 100 27.176799817328153 27.67218298739377 24.970158163673254 20.180859031604825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22521.0 1 15521.5 2 7122.0 3 5464.0 4 5258.0 5 5294.5 6 5434.5 7 5602.5 8 5657.0 9 5922.0 10 6409.5 11 9032.0 12 66126.5 13 78073.0 14 34825.0 15 36560.5 16 40621.0 17 44133.0 18 47728.5 19 52051.0 20 57939.0 21 67561.0 22 81180.5 23 96609.0 24 114092.0 25 135662.5 26 160503.0 27 190227.0 28 223434.5 29 256940.0 30 287411.5 31 318268.0 32 353958.0 33 386818.0 34 420019.5 35 460147.5 36 503592.5 37 544236.5 38 591330.0 39 637297.0 40 674048.0 41 721871.5 42 776509.0 43 809234.0 44 832720.5 45 871217.5 46 916400.0 47 959754.0 48 1000187.5 49 1033282.5 50 1055003.0 51 1070975.0 52 1080588.0 53 1074094.5 54 1052143.5 55 1016832.0 56 966192.0 57 896069.5 58 801872.5 59 688099.5 60 563820.0 61 449464.5 62 351628.5 63 264558.0 64 195601.0 65 144592.0 66 104771.5 67 76884.0 68 57647.0 69 44111.5 70 34860.0 71 29184.5 72 24985.0 73 19652.0 74 14946.0 75 11911.5 76 9132.5 77 6111.0 78 4118.0 79 2929.5 80 1978.5 81 1435.0 82 1051.5 83 750.5 84 604.5 85 485.0 86 397.0 87 351.5 88 289.5 89 240.0 90 199.0 91 166.5 92 151.5 93 132.0 94 114.0 95 97.0 96 77.5 97 60.5 98 44.0 99 36.5 100 31.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13401358259237126 2 0.050103296061609265 3 0.018307967324707672 4 0.07536902919419475 5 0.07906015163869226 6 0.07994232990292717 7 0.04721314718756771 8 0.044621979231530454 9 0.03258522894002406 10-11 0.02249739129921235 12-13 0.014681439522988863 14-15 0.011710085955168365 16-17 0.01666726339812853 18-19 0.016098830541675907 20-21 0.022536148084879574 22-23 0.019092330844163393 24-25 0.016530691867682117 26-27 0.014982266002215413 28-29 0.01688503962235388 30-31 0.014758953094323311 32-33 0.012031213607839649 34-35 0.009565543814915308 36-37 0.007382244888995029 38-39 0.016447641612680926 40-41 0.025304489918252713 42-43 0.025856312723705088 44-45 0.024376172623461586 46-47 0.02650595027393665 48-49 0.02306397859444272 50-51 0.023219005737111616 52-53 0.019583250129281565 54-55 0.024689918031243877 56-57 0.03109770659489156 58-59 0.023730226195674522 60-61 0.023619492522339594 62-63 0.030765505574886786 64-65 0.03149450225767504 66-67 0.024163933082902978 68-69 0.024867091908579757 70-71 0.03563594164040126 72-73 0.04027014586946789 74-75 0.04127044005192671 76-77 0.04238885015260946 78-79 0.0366860659758608 80-81 0.03201864164479369 82-83 0.035021369753392415 84-85 0.03829539536166172 86-87 0.03813852265777057 88-89 0.037636530005318906 90-91 0.040482385410026495 92-93 0.033904805213931924 94-95 0.03379960822426374 96-97 0.033897422969042926 98-99 0.3122006730392665 100 0.14854184053391348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.709203E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.35691047964937 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49557859617452 38.24942335340018 2 11.059122057044483 10.916881947061208 3 4.143262539605118 6.1349591478292345 4 2.1275952514075245 4.2004611344259315 5 1.257622375105012 3.1036177492631047 6 0.7819121664619908 2.315566128180791 7 0.5506292837628184 1.9024152186308416 8 0.4019322690398084 1.5870508017504135 9 0.29902609175847134 1.3283103637802058 >10 1.6304942277170325 14.58910168607455 >50 0.16746813543794964 5.84633408744448 >100 0.08452321939948197 6.413690606932167 >500 5.030874679794208E-4 0.16940775543828288 >1k 2.9332370524862996E-4 0.24533351106148155 >5k 1.4950364971684022E-5 0.05884045104871102 >10k+ 2.2425547457074163E-5 2.9386060576783968 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 753058 2.7796292858084093 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.005455478972967326 3.6911224444975144E-5 0.0 3.6911224444975148E-6 0.0 2 0.005462861217856322 4.429346933397018E-5 0.0 7.3822448889950295E-6 3.6911224444975148E-6 3 0.005477625707634311 5.536683666746272E-5 0.0 7.3822448889950295E-6 7.3822448889950295E-6 4 0.0054813168300788095 5.9057959111960236E-5 0.0 7.3822448889950295E-6 1.8455612222487572E-5 5 0.0054813168300788095 8.858693866794035E-5 0.0 7.3822448889950295E-6 1.8455612222487572E-5 6 0.0054923901974123016 9.596918355693537E-5 0.0 7.3822448889950295E-6 2.214673466698509E-5 7 0.005499772442301297 1.1073367333492544E-4 0.0 7.3822448889950295E-6 2.214673466698509E-5 8 0.005510845809634789 1.254981631129155E-4 0.0 7.3822448889950295E-6 2.214673466698509E-5 9 0.005510845809634789 1.3657153044640803E-4 0.0 7.3822448889950295E-6 2.58378571114826E-5 10-11 0.005523764738190531 1.4395377533540308E-4 0.0 7.3822448889950295E-6 3.322010200047763E-5 12-13 0.0055422203504130185 1.5687270389114438E-4 0.0 7.3822448889950295E-6 4.060234688947266E-5 14-15 0.005566212646302252 1.808649997803782E-4 0.0 1.1073367333492544E-5 4.244790811172142E-5 16-17 0.005610506115636222 2.067028568918608E-4 0.0 1.29189285557413E-5 4.613903055621893E-5 18-19 0.005645571778858948 2.3438627522559218E-4 0.0 1.8455612222487572E-5 4.983015300071644E-5 20-21 0.0057101664216376545 2.565330098925773E-4 0.0 2.030117344473633E-5 6.274908155645776E-5 22-23 0.005944552696863247 2.7129749967056733E-4 0.0 2.3992295889233847E-5 7.75135713344478E-5 24-25 0.007208762134103646 2.971353567820499E-4 0.0 2.58378571114826E-5 9.412362233468663E-5 26-27 0.01237264243395567 3.1374540778228877E-4 0.0 2.58378571114826E-5 1.033514284459304E-4 28-29 0.027877202262067483 3.3404658122702507E-4 0.0 2.58378571114826E-5 1.1811591822392047E-4 30-31 0.06327506650479864 3.5619331589401015E-4 0.0 2.9528979555980118E-5 1.2918928555741301E-4 32-33 0.12973557167919864 3.746489281164977E-4 0.0 4.060234688947266E-5 1.328804080019105E-4 34-35 0.2391515143014385 3.986412240057316E-4 0.0 4.060234688947266E-5 1.4026265289090556E-4 36-37 0.3976889144150512 4.170968362282191E-4 0.0 4.060234688947266E-5 1.4210821411315432E-4 38-39 0.6097457444126557 4.318613260062092E-4 0.0 4.244790811172142E-5 1.4579933655765182E-4 40-41 0.8763868931194894 4.447802545619505E-4 0.0 4.429346933397018E-5 1.513360202243981E-4 42-43 1.1993877904313557 4.7615479534017936E-4 0.0 5.3521275445213965E-5 1.550271426688956E-4 44-45 1.5807619436417277 4.853826014514232E-4 0.0 5.9057959111960236E-5 1.7163719366913442E-4 46-47 2.02000366897571 5.16757142229652E-4 0.0 5.9057959111960236E-5 1.8824724466937325E-4 48-49 2.518303353421652 5.31521632007642E-4 0.0 7.013132644545278E-5 2.0116617322511456E-4 50-51 3.069622320660357 5.425949993411347E-4 0.0 7.013132644545278E-5 2.0854841811410957E-4 52-53 3.6703063594717706 5.610506115636222E-4 0.0 7.013132644545278E-5 2.1777622422535336E-4 54-55 4.31947329159166 5.813517850083586E-4 0.0 7.75135713344478E-5 2.288495915588459E-4 56-57 5.013214218351301 6.145718870088361E-4 0.0 8.858693866794035E-5 2.417685201145872E-4 58-59 5.753922832655951 6.477919890093139E-4 0.0 9.227806111243786E-5 2.4361408133683596E-4 60-61 6.532219992374141 6.754754073430452E-4 0.0 9.781474477918414E-5 2.4915076500358225E-4 62-63 7.345776599243393 6.99467703232279E-4 0.0 9.96603060014329E-5 2.565330098925773E-4 64-65 8.18905412403574 7.179233154547666E-4 0.0 9.96603060014329E-5 2.805253057818111E-4 66-67 9.061633624353732 7.382244888995029E-4 0.0 9.96603060014329E-5 2.934442343375524E-4 68-69 9.960316742599208 7.419156113440004E-4 0.0 1.0150586722368164E-4 2.989809180042987E-4 70-71 10.883839269334931 7.585256623442393E-4 0.0 1.033514284459304E-4 3.0082647922654747E-4 72-73 11.828742622830404 7.788268357889756E-4 0.0 1.033514284459304E-4 3.063631628932937E-4 74-75 12.790137911407895 7.972824480114632E-4 0.0 1.0704255089042792E-4 3.137454077822887E-4 76-77 13.769819020575422 8.065102541227069E-4 0.0 1.1073367333492544E-4 3.229732138935325E-4 78-79 14.764172341459831 8.21274743900697E-4 0.0 1.1073367333492544E-4 3.3404658122702507E-4 80-81 15.767168425548029 8.43421478567682E-4 0.0 1.1627035700167171E-4 3.395832648937713E-4 82-83 16.784910543801995 8.67413774456916E-4 0.0 1.1996147944616923E-4 3.4696550978276637E-4 84-85 17.810189934087624 8.96942754012896E-4 3.6911224444975148E-6 1.254981631129155E-4 3.543477546717614E-4 86-87 18.84674754900242 9.264717335688761E-4 3.6911224444975148E-6 1.347259692241593E-4 3.635755607830052E-4 88 19.625583612597506 9.412362233468662E-4 3.6911224444975148E-6 1.4764489777990058E-4 3.72803366894249E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 105930 0.0 78.63372 8 GTGGTAT 106730 0.0 78.10384 6 AGTGGTA 107320 0.0 77.786064 5 TGGTATC 107750 0.0 77.20683 7 GTATCAA 108380 0.0 76.59589 9 AAGCAGT 112875 0.0 74.97815 1 CAGTGGT 116330 0.0 72.07329 4 GCAGTGG 117170 0.0 71.917786 3 AGCAGTG 126015 0.0 67.29254 2 CAACGCA 104440 0.0 41.999893 12-13 ATCAACG 105900 0.0 41.481026 10-11 ACGCAGA 107710 0.0 40.68562 14-15 AGAGTAC 107440 0.0 40.226967 18-19 TCAACGC 104025 0.0 39.841373 12-13 GAGTACT 100050 0.0 39.241467 20-21 AGTACTT 107095 0.0 38.913055 20-21 AACGCAG 106365 0.0 38.838924 14-15 TATCAAC 110670 0.0 37.521465 10-11 CGCAGAG 109890 0.0 37.47407 16-17 GTACTTT 105375 0.0 37.165527 22-23 >>END_MODULE