FastQCFastQC Report
Thu 26 May 2016
SRR1660653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27092030
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7455112.7517723847197866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050550.078.4333348
AGTGGTA1064000.077.565485
GTGGTAT1064300.077.450936
TGGTATC1073950.076.66757
GTATCAA1077250.076.288749
AAGCAGT1120250.074.447351
CAGTGGT1153900.071.758514
GCAGTGG1166150.071.278643
AGCAGTG1258700.066.347092
CAACGCA1030900.042.07866312-13
ATCAACG1050500.041.39634710-11
ACGCAGA1067500.040.63284314-15
AGAGTAC1068600.040.07219718-19
TCAACGC1028800.039.82436412-13
GAGTACT984700.039.4552720-21
AACGCAG1052000.038.86471614-15
AGTACTT1061300.038.7591220-21
CGCAGAG1087300.037.4830716-17
TATCAAC1100300.037.3538310-11
GTACTTT1036900.037.3418122-23
Produced by FastQC (version 0.11.5)