##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660653_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27092030 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79584158145403 34.0 31.0 34.0 31.0 34.0 2 32.977433806178425 34.0 31.0 34.0 31.0 34.0 3 33.03143677310264 34.0 33.0 34.0 31.0 34.0 4 36.4040299674849 37.0 37.0 37.0 35.0 37.0 5 36.33150325021787 37.0 37.0 37.0 35.0 37.0 6 36.325169911593925 37.0 37.0 37.0 35.0 37.0 7 36.28668327917841 37.0 37.0 37.0 35.0 37.0 8 36.29820652051544 37.0 37.0 37.0 35.0 37.0 9 38.08039069054626 39.0 38.0 39.0 37.0 39.0 10-11 38.03509880950228 39.0 38.0 39.0 35.0 39.0 12-13 38.06299203123575 39.0 38.0 39.0 36.0 39.0 14-15 39.578688658620266 41.0 40.0 41.0 37.0 41.0 16-17 39.554758133665146 41.0 40.0 41.0 37.0 41.0 18-19 39.52553415155675 41.0 39.5 41.0 37.0 41.0 20-21 39.49267862172012 41.0 39.5 41.0 37.0 41.0 22-23 39.3838401367487 41.0 39.0 41.0 36.5 41.0 24-25 39.36864452386919 41.0 39.0 41.0 36.0 41.0 26-27 39.30465099883619 41.0 39.0 41.0 36.0 41.0 28-29 39.22480810777191 41.0 39.0 41.0 36.0 41.0 30-31 39.13571028084643 40.0 39.0 41.0 36.0 41.0 32-33 39.038876101200245 40.0 39.0 41.0 35.0 41.0 34-35 38.93770119847055 40.0 38.5 41.0 35.0 41.0 36-37 38.802317434315555 40.0 38.0 41.0 35.0 41.0 38-39 38.54893590476608 40.0 38.0 41.0 35.0 41.0 40-41 38.4269215891168 40.0 38.0 41.0 34.5 41.0 42-43 38.295372402880105 40.0 38.0 41.0 34.0 41.0 44-45 38.160719259501775 40.0 37.5 41.0 34.0 41.0 46-47 38.01413231123692 40.0 37.0 41.0 33.5 41.0 48-49 37.849501883026115 40.0 37.0 41.0 33.0 41.0 50-51 37.891204258226495 40.0 37.0 41.0 33.0 41.0 52-53 37.81035182671805 40.0 37.0 41.0 33.0 41.0 54-55 37.419664916213364 40.0 36.0 41.0 33.0 41.0 56-57 37.17624735023547 40.0 36.0 41.0 32.0 41.0 58-59 36.95340253203617 39.0 35.5 41.0 32.0 41.0 60-61 36.7120065384543 39.0 35.0 41.0 32.0 41.0 62-63 36.44002444630395 38.5 35.0 41.0 31.0 41.0 64-65 36.155173920152905 38.0 35.0 40.0 31.0 41.0 66-67 35.862254157403484 37.0 35.0 40.0 31.0 41.0 68-69 35.55382662354944 37.0 35.0 39.5 31.0 41.0 70-71 35.22357076970607 36.0 35.0 39.0 30.0 41.0 72-73 34.871441250434174 35.5 35.0 39.0 30.0 40.5 74-75 34.532176953886434 35.0 34.0 37.5 30.0 40.0 76-77 32.69431674186098 34.5 32.0 35.5 27.0 38.5 78-79 33.83696050092961 35.0 34.0 36.0 29.5 39.0 80-81 33.81713038853124 35.0 34.0 36.0 30.0 38.5 82-83 33.60728825045595 35.0 34.0 36.0 30.0 37.0 84-85 33.39546049151725 35.0 34.0 35.0 29.5 37.0 86-87 33.217208455770944 35.0 34.0 35.0 29.0 36.5 88-89 33.06259351920103 35.0 34.0 35.0 29.0 36.0 90-91 32.95951482779253 35.0 34.0 35.0 29.0 36.0 92-93 32.87183832293114 35.0 34.0 35.0 29.0 36.0 94-95 32.77791219410284 35.0 34.0 35.0 29.0 35.0 96-97 32.70677012759841 35.0 34.0 35.0 29.0 35.0 98-99 32.61504228365316 35.0 34.0 35.0 29.0 35.0 100 32.56598885354844 35.0 34.0 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3731599601964106 1101 2 0.3149919944286381 1101 3 0.3146781201573532 1101 4 0.2307325748061686 1101 5 0.24035582391715593 1101 6 0.25881851378038334 1101 7 0.26104280366189414 1101 8 0.26597536163904323 1101 9 0.3375393359441077 1101 10-11 0.34079054845998513 1101 12-13 0.3445644552109144 1101 14-15 0.4435357544187113 1101 16-17 0.4497441407029612 1101 18-19 0.437592920540375 1101 20-21 0.44804162920362245 1101 22-23 0.44001372333710265 1101 24-25 0.4530730443701856 1101 26-27 0.46145213667124807 1101 28-29 0.4862779709457783 1101 30-31 0.49785810501927585 1101 32-33 0.4801713878152327 1101 34-35 0.504395709522079 1101 36-37 0.531684824378722 1101 38-39 0.48752698146389406 1101 40-41 0.5173784965858204 1101 42-43 0.5460942748540631 1101 44-45 0.5493212698295125 1101 46-47 0.5273539531500546 1101 48-49 0.5668854039724138 1101 50-51 0.5292575648916795 1101 52-53 0.555900856686776 1101 54-55 0.45547678499836053 1101 56-57 0.45913545902689634 1101 58-59 0.4595973188663294 1101 60-61 0.41466256308441984 1101 62-63 0.3505742371584688 1101 64-65 0.3408729095483167 1101 66-67 0.35436071840781835 1101 68-69 0.3397951545585869 1101 70-71 0.3628829430424929 1101 72-73 0.3769688372030302 1101 74-75 0.37198804811106356 1101 76-77 0.39919027409953856 1101 78-79 0.44877715268437157 1101 80-81 0.4197234460544621 1101 82-83 0.41593042606718456 1101 84-85 0.3637839528727227 1101 86-87 0.3952679875160712 1101 88-89 0.44171852408238266 1101 90-91 0.3898759902720954 1101 92-93 0.34321524752621 1101 94-95 0.34433589519985475 1101 96-97 0.35081113041069756 1101 98-99 0.3181367195136886 1101 100 0.34583584349424257 1102 1 0.32223193919951854 1102 2 0.271464424054642 1102 3 0.27870658279277905 1102 4 0.21178948455549573 1102 5 0.21680689954224874 1102 6 0.23465318296614868 1102 7 0.23182982118161988 1102 8 0.2228805428733054 1102 9 0.2758785593396098 1102 10-11 0.30120365230276036 1102 12-13 0.2845705301341326 1102 14-15 0.3726125960794988 1102 16-17 0.37851754889916833 1102 18-19 0.3846426446140043 1102 20-21 0.38335749178890666 1102 22-23 0.39074549553676974 1102 24-25 0.38845809332929093 1102 26-27 0.4319656172340487 1102 28-29 0.42448767906282825 1102 30-31 0.4443610259561126 1102 32-33 0.4293117329302376 1102 34-35 0.43198785784809246 1102 36-37 0.4253183433623988 1102 38-39 0.41277225507280235 1102 40-41 0.426789914473126 1102 42-43 0.4157389541582788 1102 44-45 0.43501333806669606 1102 46-47 0.42387481674990113 1102 48-49 0.4071215612311221 1102 50-51 0.39040583980948895 1102 52-53 0.42090495403102324 1102 54-55 0.29487798473338245 1102 56-57 0.26356219961078864 1102 58-59 0.27187460840824684 1102 60-61 0.20550812396626839 1102 62-63 0.24665021516501895 1102 64-65 0.24632550662331454 1102 66-67 0.20308954355596143 1102 68-69 0.19171216331261576 1102 70-71 0.23634779623543523 1102 72-73 0.2580334533357487 1102 74-75 0.2887738835019462 1102 76-77 0.28545351087799986 1102 78-79 0.34260486527864487 1102 80-81 0.31868275347243014 1102 82-83 0.2970574126956649 1102 84-85 0.32977516179489896 1102 86-87 0.3314977680314328 1102 88-89 0.34660159540585767 1102 90-91 0.32711377865108915 1102 92-93 0.2844747653685502 1102 94-95 0.33555264386061623 1102 96-97 0.3338744755906262 1102 98-99 0.32898186233386184 1102 100 0.35570216006163946 1103 1 0.27527795851333536 1103 2 0.2581344604705791 1103 3 0.25573601223383235 1103 4 0.16003701659161607 1103 5 0.17549357030339507 1103 6 0.2018301765417334 1103 7 0.19741707685849974 1103 8 0.17160255070581343 1103 9 0.2524141304060734 1103 10-11 0.25513954072961553 1103 12-13 0.2627172126434232 1103 14-15 0.32750986573842766 1103 16-17 0.3177670288699943 1103 18-19 0.31215362632491406 1103 20-21 0.33846000388550834 1103 22-23 0.3508809841644833 1103 24-25 0.34093109535793076 1103 26-27 0.3238182038117259 1103 28-29 0.33521688731519106 1103 30-31 0.3526907661484415 1103 32-33 0.3258571497040492 1103 34-35 0.35875331856316706 1103 36-37 0.342694909137677 1103 38-39 0.3593828837012367 1103 40-41 0.3153720029037217 1103 42-43 0.3244435909675971 1103 44-45 0.32172664247509175 1103 46-47 0.3205197550536383 1103 48-49 0.33241962653521284 1103 50-51 0.3312462271398644 1103 52-53 0.3396566513449102 1103 54-55 0.24487932714266236 1103 56-57 0.2291761181714378 1103 58-59 0.25891227348907364 1103 60-61 0.20235395423097202 1103 62-63 0.2048154052157969 1103 64-65 0.20477400268224955 1103 66-67 0.22320327362339754 1103 68-69 0.20958508080241955 1103 70-71 0.1884516768894855 1103 72-73 0.21836775030756428 1103 74-75 0.22065858083909262 1103 76-77 0.13415377044476884 1103 78-79 0.27307986815596763 1103 80-81 0.2913811381958098 1103 82-83 0.26869052153232786 1103 84-85 0.2537052770382644 1103 86-87 0.2537956371214065 1103 88-89 0.292632144688028 1103 90-91 0.22024601893659224 1103 92-93 0.23743167300111878 1103 94-95 0.26620199317300575 1103 96-97 0.27559311154631416 1103 98-99 0.2933599202011692 1103 100 0.283967794033984 1104 1 0.20239308821305002 1104 2 0.18149880764691062 1104 3 0.16899403419532888 1104 4 0.12144856179528318 1104 5 0.130762230329033 1104 6 0.1320238730841865 1104 7 0.14038401679067647 1104 8 0.14038249967713057 1104 9 0.16924640072564756 1104 10-11 0.1823584153135016 1104 12-13 0.19111703566426286 1104 14-15 0.21375639606296915 1104 16-17 0.252020855786796 1104 18-19 0.23033102036684028 1104 20-21 0.23968543920342 1104 22-23 0.2544768300420017 1104 24-25 0.23507894114920447 1104 26-27 0.21272826680700518 1104 28-29 0.22473969076553146 1104 30-31 0.2262751106187011 1104 32-33 0.24689533808155772 1104 34-35 0.2619336988382912 1104 36-37 0.2942349572647416 1104 38-39 0.2564194989817352 1104 40-41 0.26203166311538695 1104 42-43 0.24465555525333826 1104 44-45 0.2758896443225254 1104 46-47 0.2576631446133746 1104 48-49 0.27040353673553597 1104 50-51 0.252243001023821 1104 52-53 0.2737032155124268 1104 54-55 0.1659630870058706 1104 56-57 0.1800442953853718 1104 58-59 0.1866198475747467 1104 60-61 0.11960823508415785 1104 62-63 0.09462761840335077 1104 64-65 0.14348937205983958 1104 66-67 0.13885341425331887 1104 68-69 0.12430899591591782 1104 70-71 0.16382796983508996 1104 72-73 0.19512337055247997 1104 74-75 0.20123030009934695 1104 76-77 0.16815464198695906 1104 78-79 0.24550560663267618 1104 80-81 0.22977651149236777 1104 82-83 0.24408600276937875 1104 84-85 0.22511281525191862 1104 86-87 0.24581056857753225 1104 88-89 0.2893903341858248 1104 90-91 0.28375171925706155 1104 92-93 0.3063956999528372 1104 94-95 0.27750337309206685 1104 96-97 0.30623922165536754 1104 98-99 0.2858155926763004 1104 100 0.30129456499299323 1105 1 0.1893214597101931 1105 2 0.14681952811450572 1105 3 0.1431401962299219 1105 4 0.12227294101231223 1105 5 0.1079934040753372 1105 6 0.1319958254234166 1105 7 0.12606748376367705 1105 8 0.13183026942218845 1105 9 0.16742445246790538 1105 10-11 0.16933458521630484 1105 12-13 0.14112922412412132 1105 14-15 0.1984630410250503 1105 16-17 0.1889460250381063 1105 18-19 0.21849312610385851 1105 20-21 0.20028770180365996 1105 22-23 0.20975637160698568 1105 24-25 0.2229739408356224 1105 26-27 0.19272602289072438 1105 28-29 0.19872370657050453 1105 30-31 0.22053809019513437 1105 32-33 0.24264806264412186 1105 34-35 0.20531975327039476 1105 36-37 0.20354383556593802 1105 38-39 0.18849871692442122 1105 40-41 0.21299353171728796 1105 42-43 0.22882938601571112 1105 44-45 0.24328160404206045 1105 46-47 0.2546405420494864 1105 48-49 0.20722348589073647 1105 50-51 0.21313658732668728 1105 52-53 0.1866498161205996 1105 54-55 0.11082267438665383 1105 56-57 0.13577914949256353 1105 58-59 0.12052486015884512 1105 60-61 0.11872763816457166 1105 62-63 0.05314925719603991 1105 64-65 0.11354679378475652 1105 66-67 0.10223731377979561 1105 68-69 0.05534014282596189 1105 70-71 0.06257564385026626 1105 72-73 0.08515236751073729 1105 74-75 0.12034240206616431 1105 76-77 0.05810635878009407 1105 78-79 0.21590326051342146 1105 80-81 0.1882511861387286 1105 82-83 0.18635088160956315 1105 84-85 0.18810195326094004 1105 86-87 0.22193625994280097 1105 88-89 0.23147061332706187 1105 90-91 0.20421897553156754 1105 92-93 0.18303144678542793 1105 94-95 0.18532517638173118 1105 96-97 0.19652275734031122 1105 98-99 0.2079000270495257 1105 100 0.2168391508504044 1106 1 0.2355752998117353 1106 2 0.20188098311400893 1106 3 0.16711067524484235 1106 4 0.12648121804198809 1106 5 0.1358709742675117 1106 6 0.14099571922034215 1106 7 0.14391234789061969 1106 8 0.1668000037142363 1106 9 0.20273279672579037 1106 10-11 0.19619503683169626 1106 12-13 0.1780782251753834 1106 14-15 0.25974306571564654 1106 16-17 0.25044151581741403 1106 18-19 0.26948761778621133 1106 20-21 0.23452859669723836 1106 22-23 0.2335088613967784 1106 24-25 0.24936377148256383 1106 26-27 0.24730935800133835 1106 28-29 0.2572559001643313 1106 30-31 0.261851475592799 1106 32-33 0.26491645842314426 1106 34-35 0.24889147407328238 1106 36-37 0.25103441917028135 1106 38-39 0.2501848039092991 1106 40-41 0.240342907884461 1106 42-43 0.23673297704609553 1106 44-45 0.2714647556939056 1106 46-47 0.2594221367598948 1106 48-49 0.2454796631829268 1106 50-51 0.22429840054650185 1106 52-53 0.2210987504786317 1106 54-55 0.10627140805052449 1106 56-57 0.14378344366828344 1106 58-59 0.1443712355649538 1106 60-61 0.07602576502137026 1106 62-63 0.0536139950635004 1106 64-65 0.12555867804639576 1106 66-67 0.0976814656737588 1106 68-69 0.1279909202881555 1106 70-71 0.07331832182049425 1106 72-73 0.11962658203270138 1106 74-75 0.15030561921978602 1106 76-77 0.08236465789264003 1106 78-79 0.2276145860919243 1106 80-81 0.2583189099392982 1106 82-83 0.25934867346759205 1106 84-85 0.2630674390684504 1106 86-87 0.2723056698853057 1106 88-89 0.29713327534007306 1106 90-91 0.2972396223784344 1106 92-93 0.29222128250755475 1106 94-95 0.30347822643793876 1106 96-97 0.2774811349158881 1106 98-99 0.3136134218167683 1106 100 0.29651598117801115 1107 1 0.18114576016103712 1107 2 0.1588050273799695 1107 3 0.15404585743516463 1107 4 0.10968832353785274 1107 5 0.11536414176985232 1107 6 0.11565241573652685 1107 7 0.12647268176509385 1107 8 0.14474442146374855 1107 9 0.1898792090378194 1107 10-11 0.19365837862606128 1107 12-13 0.16309798681994891 1107 14-15 0.21536201988657666 1107 16-17 0.17895456150508693 1107 18-19 0.22526257462694588 1107 20-21 0.20401969072063508 1107 22-23 0.20756528058146273 1107 24-25 0.19548281268933465 1107 26-27 0.1904361598168336 1107 28-29 0.23701935570424837 1107 30-31 0.22839301993233363 1107 32-33 0.21525514020817127 1107 34-35 0.23876484026578026 1107 36-37 0.20567653478137515 1107 38-39 0.23937097895144888 1107 40-41 0.23220809315246527 1107 42-43 0.21027836205431072 1107 44-45 0.19496280341439132 1107 46-47 0.21509247682671173 1107 48-49 0.20923126543346626 1107 50-51 0.21682272140449754 1107 52-53 0.23429356373464572 1107 54-55 0.15579632699331825 1107 56-57 0.1583031796531884 1107 58-59 0.13305351972829982 1107 60-61 0.044673957315978896 1107 62-63 0.10168424725148384 1107 64-65 0.10487599175828421 1107 66-67 0.108710739439978 1107 68-69 0.05397538280232794 1107 70-71 0.06553621295634571 1107 72-73 0.10726855507363098 1107 74-75 0.1323246634288111 1107 76-77 0.0743772198918009 1107 78-79 0.22616248900164493 1107 80-81 0.2419090137655857 1107 82-83 0.223164225240005 1107 84-85 0.24895435711707137 1107 86-87 0.25175426202711293 1107 88-89 0.27197874791653476 1107 90-91 0.27825068886981086 1107 92-93 0.2662472835194478 1107 94-95 0.2982582727673275 1107 96-97 0.27084150629637804 1107 98-99 0.2842999755424245 1107 100 0.2898102142000738 1108 1 0.04456385525575968 1108 2 0.028773695079479467 1108 3 0.016014431645956506 1108 4 0.03940229023784525 1108 5 0.039933515880754555 1108 6 0.025940636029943676 1108 7 0.03826430650149604 1108 8 0.03521572728458722 1108 9 0.031081059589972426 1108 10-11 0.04699295839683515 1108 12-13 0.024584317939854827 1108 14-15 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-0.4493949680840359 2316 62-63 -0.4319851068966898 2316 64-65 -0.44301897992365014 2316 66-67 -0.45951388579030095 2316 68-69 -0.42019820419940146 2316 70-71 -0.44948687329701187 2316 72-73 -0.490184123907234 2316 74-75 -0.48745374287714327 2316 76-77 -0.5336041945705503 2316 78-79 -0.6926580870517753 2316 80-81 -0.670042910225007 2316 82-83 -0.6649541384529627 2316 84-85 -0.6483286549358667 2316 86-87 -0.6747120665443376 2316 88-89 -0.7593905729292771 2316 90-91 -0.6368735352547787 2316 92-93 -0.5567059649776311 2316 94-95 -0.5497397839522762 2316 96-97 -0.6068080385403292 2316 98-99 -1.4072362099988212 2316 100 -0.9846862792846238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 21.0 7 282.0 8 441.0 9 189.0 10 70.0 11 57.0 12 96.0 13 318.0 14 1573.0 15 5005.0 16 11753.0 17 19873.0 18 25762.0 19 31052.0 20 38697.0 21 52270.0 22 77238.0 23 125804.0 24 205128.0 25 270675.0 26 284905.0 27 261154.0 28 250495.0 29 274426.0 30 326241.0 31 397456.0 32 494620.0 33 640941.0 34 914965.0 35 1518972.0 36 3109077.0 37 7065043.0 38 8705463.0 39 1979912.0 40 2056.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.133636388796482 30.3125965223484 27.7688387698452 23.78492831900992 2 23.110615892061947 30.811029545258524 25.632010998738743 20.44634356394079 3 25.100954044418227 24.989382486288402 26.485844729981473 23.423818739311894 4 21.745579788594654 27.105905316065275 24.346551365844494 26.801963529495577 5 23.462055076714446 28.935369553333583 25.785306601240293 21.817268768711685 6 26.962785734402335 26.67173703853126 25.126592580917713 21.2388846461487 7 22.92031641778043 25.488333653845803 28.83763970437062 22.75371022400315 8 26.042828831947993 26.371770590834277 25.079907264239704 22.50549331297802 9 25.300485050400432 25.25532785841445 26.513288225356312 22.930898865828805 10-11 22.47510061077003 27.95613876110428 27.086639502466227 22.482121125659464 12-13 22.381303652771685 26.358464094421862 27.11414759248384 24.14608466032261 14-15 22.366917503044252 29.476393980074583 25.631554741375968 22.525133775505193 16-17 24.05707139701233 26.13594477785533 25.712661620410138 24.0943222047222 18-19 22.401165866355313 27.736912019198613 25.74806337349728 24.11385874094879 20-21 24.013757503650375 27.826190952312444 25.72935375519111 22.43069778884607 22-23 23.959417686575442 26.284777956076898 27.289234950115166 22.46656940723249 24-25 22.50902043761414 27.615790335471104 25.63120731828495 24.24398190862981 26-27 22.568906688400535 26.07748877114162 28.869856168841412 22.483748371616432 28-29 22.599644620045716 26.150618413648747 28.81035164040464 22.439385325900894 30-31 22.5306665751685 26.084803777865357 28.824540222165947 22.559989424800197 32-33 22.6288884371002 26.212783919735262 28.645172578920462 22.513155064244074 34-35 22.628717683997156 26.34339503485864 28.548575370144185 22.47931191100002 36-37 22.586559425938876 26.289453246676636 28.622770780216676 22.50121654716781 38-39 22.64123036698984 26.279515434057497 28.638642233089833 22.44061196586283 40-41 22.640178200536962 26.354414677082595 28.59338250680136 22.41202461557909 42-43 22.68011560148162 26.37888157434692 28.516468943159406 22.424533881012053 44-45 22.76780448020483 26.447199218109237 28.445147107403052 22.339849194282877 46-47 22.770536600079172 26.58408890606003 28.33069290134666 22.314681592514138 48-49 22.822335700764164 26.62006661313388 28.192080532051538 22.365517154050412 50-51 22.870830255952267 26.6886490016087 28.08990609965954 22.35061464277949 52-53 22.935293023858886 26.829655720079586 27.85839688665559 22.376654369405934 54-55 23.089558661003235 26.99789833822002 27.396273833535773 22.516269167240978 56-57 23.326900085201462 27.307534718792866 26.77600934581615 22.589555850189527 58-59 23.417643810633315 27.600647629794295 26.37410079385269 22.6076077657197 60-61 23.444370413563608 27.731466892640537 26.148878462958734 22.675284230837118 62-63 23.40896507117932 27.85975691092969 26.034731438251413 22.69654657963958 64-65 23.40693905399935 27.958940422326883 25.921152249690742 22.712968273983027 66-67 23.34696105661916 27.914572296737795 25.941293800615757 22.79717284602729 68-69 23.296025148689797 27.898291602622066 25.97304543439185 22.832637814296287 70-71 23.27187490097982 27.897013769279898 25.95997224886765 22.87113908087263 72-73 23.254586103371146 27.90231616019272 25.888362386142294 22.95473535029384 74-75 23.297371768265496 28.00359920229804 25.74230963089348 22.956719398542987 76-77 23.304428013460583 28.1115715014668 25.564416047228498 23.019584437844117 78-79 23.350352913907567 28.21764915241433 25.322135120788893 23.10986281288921 80-81 23.388006672227164 28.328104779296375 25.111629058070882 23.17225949040558 82-83 23.36619292762746 28.44446276891517 24.913049145769094 23.276295157688274 84-85 23.361583086137617 28.462958938518746 24.78688845301851 23.38856952232513 86-87 23.376684880385966 28.48170378580248 24.68803908019447 23.45357225361708 88-89 23.385327900008317 28.484897589008934 24.615201049113182 23.51457346186957 90-91 23.407766507026345 28.452835824426987 24.5431623061108 23.59623536243587 92-93 23.446461336604557 28.42795826512515 24.511787177718908 23.613793220551386 94-95 23.481471584972454 28.409762056773303 24.485464223470817 23.623302134783426 96-97 23.46745722029616 28.352117324062508 24.491492911776422 23.688932543864905 98-99 23.443993622157475 28.334469570861366 24.508229144698376 23.71330766228278 100 23.378891922141662 28.344611506711942 24.484265617464214 23.792230953682182 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22391.0 1 15246.5 2 6745.0 3 5117.0 4 4778.5 5 4743.5 6 4867.5 7 5048.5 8 5153.5 9 5410.5 10 5825.5 11 8443.0 12 65033.0 13 76170.0 14 32522.5 15 34198.0 16 38544.5 17 42539.0 18 46732.5 19 51767.0 20 58220.0 21 68378.0 22 82734.0 23 98861.0 24 116986.5 25 139693.0 26 165701.5 27 196711.5 28 232146.0 29 267284.5 30 298504.0 31 329247.5 32 364580.5 33 398098.0 34 429595.5 35 466630.0 36 508617.5 37 545774.0 38 590252.5 39 637358.0 40 674960.5 41 723221.0 42 779988.0 43 815624.0 44 840255.5 45 878894.0 46 919258.0 47 951284.5 48 984147.5 49 1019173.5 50 1051838.0 51 1080661.5 52 1096684.5 53 1089921.0 54 1062654.5 55 1021571.5 56 964896.0 57 888044.0 58 789419.0 59 673767.0 60 548596.5 61 435618.0 62 340976.5 63 255476.5 64 188141.5 65 139114.0 66 100276.5 67 73140.5 68 54341.5 69 40934.0 70 32017.0 71 26674.5 72 22999.5 73 17879.0 74 13337.0 75 10368.5 76 7630.0 77 4937.0 78 3099.0 79 1961.5 80 1138.0 81 675.0 82 417.5 83 231.5 84 130.0 85 72.5 86 47.5 87 32.0 88 15.5 89 8.5 90 4.5 91 5.0 92 7.5 93 5.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10703147752309443 2 0.0732134136866082 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.7314306089281607E-4 20-21 0.002334634946144678 22-23 8.415759173454335E-4 24-25 8.046646929004582E-4 26-27 0.007007595960878532 28-29 0.006900553409988103 30-31 0.008247813102229697 32-33 0.008037419122893338 34-35 0.0075520365214419155 36-37 0.011324363659718376 38-39 0.013978280697312086 40-41 0.017695240998921086 42-43 0.024228527725681685 44-45 0.01231173891362146 46-47 0.019332253803055734 48-49 0.01690164967335412 50-51 0.019490972068169125 52-53 0.021838525942869547 54-55 0.1102667463456965 56-57 0.10313365222170505 58-59 0.10034316365366493 60-61 0.14304945033650118 62-63 0.09332818544789741 64-65 0.07221496506537163 66-67 0.08243568311418524 68-69 0.0753025889901938 70-71 0.052472996670976664 72-73 0.0309666717481119 74-75 0.020199667577512646 76-77 0.012189931872953043 78-79 0.00867229218334691 80-81 0.005630807289080959 82-83 5.370583156743884E-4 84-85 0.002371546170589653 86-87 0.001681306273468618 88-89 0.0021316232116973147 90-91 0.0030138014759322207 92-93 0.004641586473955625 94-95 0.0020171984159178917 96-97 0.0011018000496825081 98-99 0.0015631903552446973 100 0.001727445304024837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.709203E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.63769257114564 #Duplication Level Percentage of deduplicated Percentage of total 1 76.83860651023822 37.37252521040196 2 11.451646156589263 11.139632903954602 3 4.214615067515181 6.149674558785649 4 2.1256242078706746 4.135418269767953 5 1.2918177341119934 3.141551690484655 6 0.837970331678714 2.4454166025558144 7 0.5781814108408663 1.9684986797580513 8 0.40912349213361365 1.591905810722258 9 0.31000516922037163 1.3570142504405776 >10 1.680016913189895 14.734719597847818 >50 0.17215794985679472 5.910022228812601 >100 0.08930479908765036 6.661595419604059 >500 6.257480910239207E-4 0.2052776719118659 >1k 2.666582971783732E-4 0.21938465463103227 >5k 7.570255716870514E-6 0.023439597373612456 >10k+ 3.0281022867124664E-5 2.943922852947499 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 745511 2.7517723847197866 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.005485007952523307 1.1073367333492544E-5 0.0 3.6911224444975148E-6 7.3822448889950295E-6 2 0.005496081319856799 2.58378571114826E-5 0.0 3.6911224444975148E-6 7.3822448889950295E-6 3 0.0055034635647457945 3.6911224444975144E-5 0.0 3.6911224444975148E-6 1.1073367333492544E-5 4 0.005510845809634789 6.644020400095527E-5 0.0 3.6911224444975148E-6 2.58378571114826E-5 5 0.005518228054523784 9.596918355693537E-5 0.0 3.6911224444975148E-6 3.3220102000477635E-5 6 0.005529301421857277 1.033514284459304E-4 0.0 3.6911224444975148E-6 3.3220102000477635E-5 7 0.005532992544301774 1.1073367333492544E-4 0.0 3.6911224444975148E-6 3.3220102000477635E-5 8 0.005540374789190769 1.1073367333492544E-4 0.0 7.3822448889950295E-6 3.6911224444975144E-5 9 0.005547757034079765 1.1073367333492544E-4 0.0 7.3822448889950295E-6 3.6911224444975144E-5 10-11 0.00556990376874675 1.1996147944616923E-4 0.0 7.3822448889950295E-6 4.244790811172142E-5 12-13 0.005592050503413735 1.347259692241593E-4 0.0 7.3822448889950295E-6 4.429346933397018E-5 14-15 0.005623425044191964 1.4949045900214934E-4 0.0 1.1073367333492544E-5 4.798459177846769E-5 16-17 0.0056658729523036844 1.5502714266889562E-4 0.0 1.4764489777990059E-5 6.274908155645775E-5 18-19 0.005697247493081914 1.7163719366913442E-4 0.0 1.4764489777990059E-5 7.935913255669656E-5 20-21 0.005763687697082869 2.0485729566961208E-4 0.0 2.030117344473633E-5 9.04324998901891E-5 22-23 0.005972236115196979 2.528418874480797E-4 0.0 2.3992295889233847E-5 9.227806111243786E-5 24-25 0.007253055603437615 2.842164282263086E-4 0.0 2.768341833373136E-5 9.781474477918414E-5 26-27 0.012405862535956148 3.026720404487962E-4 0.0 3.137454077822888E-5 1.0519698966817917E-4 28-29 0.02796763476195767 3.2850989756027875E-4 0.0 3.3220102000477635E-5 1.1442479577942295E-4 30-31 0.06347254155557926 3.506566322272639E-4 0.0 3.6911224444975144E-5 1.1996147944616923E-4 32-33 0.13042027489265293 3.7095780567200024E-4 0.0 3.6911224444975144E-5 1.2734372433516425E-4 34-35 0.24049877399368008 3.931045403389853E-4 0.0 3.87567856672239E-5 1.2918928555741301E-4 36-37 0.3998205376267485 4.152512750059704E-4 0.0 4.244790811172142E-5 1.384170916686568E-4 38-39 0.612656194460142 4.3739800967295545E-4 0.0 4.429346933397018E-5 1.4395377533540308E-4 40-41 0.8805006490838818 4.5031693822869675E-4 0.0 4.798459177846769E-5 1.4949045900214934E-4 42-43 1.2049669220062138 4.7800035656242815E-4 0.0 5.536683666746272E-5 1.7163719366913442E-4 44-45 1.587129129858486 5.09374897340657E-4 0.0 5.536683666746272E-5 1.808649997803782E-4 46-47 2.026584940294249 5.296760707853934E-4 0.0 5.7212397889711475E-5 1.9193836711387077E-4 48-49 2.525189142341862 5.481316830078809E-4 0.0 6.090352033420899E-5 2.030117344473633E-4 50-51 3.0768679940189054 5.758151013416123E-4 0.0 6.274908155645775E-5 2.030117344473633E-4 52-53 3.678570782625001 5.942707135640999E-4 0.0 7.013132644545278E-5 2.067028568918608E-4 54-55 4.328029313418005 6.164174482310849E-4 0.0 7.566801011219905E-5 2.1223954055860707E-4 56-57 5.023015994002664 6.607109175650551E-4 0.0 7.935913255669656E-5 2.2146734666985088E-4 58-59 5.764101102796653 6.828576522320401E-4 0.0 8.489581622344283E-5 2.2515846911434837E-4 60-61 6.544408078685872 6.957765807877815E-4 0.0 8.858693866794035E-5 2.3069515278109466E-4 62-63 7.359880378103819 7.289966827882591E-4 0.0 8.858693866794035E-5 2.3438627522559218E-4 64-65 8.205931781413206 7.566801011219905E-4 0.0 8.858693866794035E-5 2.3623183644784094E-4 66-67 9.08107661183012 7.75135713344478E-4 0.0 8.858693866794035E-5 2.3992295889233844E-4 68-69 9.983205392877537 7.917457643447169E-4 0.0 1.0150586722368164E-4 2.4730520378133346E-4 70-71 10.910690339557426 8.083558153449557E-4 0.0 1.0519698966817917E-4 2.528418874480797E-4 72-73 11.8601559203943 8.360392336786871E-4 0.0 1.0704255089042792E-4 2.6022413233707476E-4 74-75 12.826172494272301 8.581859683456721E-4 0.0 1.0704255089042792E-4 2.65760816003821E-4 76-77 13.810749877362458 8.914060703461498E-4 0.0 1.0704255089042792E-4 2.786797445595623E-4 78-79 14.81035197436294 9.209350499021299E-4 0.0 1.1073367333492544E-4 2.8421642822630864E-4 80-81 15.819565754208895 9.356995396801199E-4 0.0 1.1257923455717419E-4 2.897531118930549E-4 82-83 16.843202595006723 9.689196416805975E-4 0.0 1.1627035700167171E-4 2.934442343375524E-4 84-85 17.875166608039336 0.0010058308661255728 0.0 1.1811591822392047E-4 3.026720404487962E-4 86-87 18.919416522128465 0.0010316687232370552 0.0 1.1996147944616923E-4 3.026720404487962E-4 88 19.705378297602653 0.0010335142844593041 0.0 1.2180704066841798E-4 3.026720404487962E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 105055 0.0 78.433334 8 AGTGGTA 106400 0.0 77.56548 5 GTGGTAT 106430 0.0 77.45093 6 TGGTATC 107395 0.0 76.6675 7 GTATCAA 107725 0.0 76.28874 9 AAGCAGT 112025 0.0 74.44735 1 CAGTGGT 115390 0.0 71.75851 4 GCAGTGG 116615 0.0 71.27864 3 AGCAGTG 125870 0.0 66.34709 2 CAACGCA 103090 0.0 42.078663 12-13 ATCAACG 105050 0.0 41.396347 10-11 ACGCAGA 106750 0.0 40.632843 14-15 AGAGTAC 106860 0.0 40.072197 18-19 TCAACGC 102880 0.0 39.824364 12-13 GAGTACT 98470 0.0 39.45527 20-21 AACGCAG 105200 0.0 38.864716 14-15 AGTACTT 106130 0.0 38.75912 20-21 CGCAGAG 108730 0.0 37.48307 16-17 TATCAAC 110030 0.0 37.35383 10-11 GTACTTT 103690 0.0 37.34181 22-23 >>END_MODULE