FastQCFastQC Report
Thu 26 May 2016
SRR1660652_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660652_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43017845
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11936502.7747786993978893No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT469610.10916632388256549No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1694100.078.458958
GTGGTAT1701050.078.086226
AGTGGTA1718100.077.373495
GTATCAA1715200.077.2910469
TGGTATC1721650.077.0209057
AAGCAGT1815100.074.258031
CAGTGGT1896850.070.374464
GCAGTGG1913600.070.372273
AGCAGTG2011750.067.21942
CAACGCA1661250.042.67695212-13
ATCAACG1690250.042.0549110-11
ACGCAGA1721000.041.13289314-15
AGAGTAC1695750.040.9667818-19
TCAACGC1652100.040.197112-13
GAGTACT1594250.039.4646220-21
AGTACTT1701650.039.42325620-21
AACGCAG1686250.039.2565414-15
TATCAAC1751800.037.86016510-11
CGCAGAG1771450.037.22661216-17
GTACTTT1689050.037.17866522-23
Produced by FastQC (version 0.11.5)