##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660652_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43017845 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20976987573413 34.0 31.0 34.0 30.0 34.0 2 32.371973956389496 34.0 31.0 34.0 30.0 34.0 3 32.40510146428767 34.0 31.0 34.0 30.0 34.0 4 35.81234348210609 37.0 37.0 37.0 35.0 37.0 5 35.79342503093774 37.0 35.0 37.0 35.0 37.0 6 35.752450314514824 37.0 36.0 37.0 35.0 37.0 7 35.71772212206353 37.0 35.0 37.0 35.0 37.0 8 35.75172129147799 37.0 35.0 37.0 35.0 37.0 9 37.427492125651575 39.0 38.0 39.0 35.0 39.0 10-11 37.366162356575515 39.0 37.5 39.0 34.5 39.0 12-13 37.39298784027884 39.0 38.0 39.0 35.0 39.0 14-15 38.805418309541075 40.0 39.0 41.0 35.0 41.0 16-17 38.70389276822212 40.0 38.5 41.0 35.0 41.0 18-19 38.63446151242583 40.0 38.0 41.0 34.0 41.0 20-21 38.54681342126739 40.0 38.0 41.0 34.0 41.0 22-23 38.36955106653994 40.0 38.0 41.0 34.0 41.0 24-25 38.329535266120374 40.0 38.0 41.0 34.0 41.0 26-27 38.25781164770109 40.0 38.0 41.0 34.0 41.0 28-29 38.25562278863574 40.0 38.0 41.0 34.0 41.0 30-31 38.242014401232794 40.0 38.0 41.0 34.0 41.0 32-33 38.124165285360064 40.0 38.0 41.0 33.0 41.0 34-35 38.06796688676525 40.0 38.0 41.0 33.0 41.0 36-37 38.01848868765974 40.0 38.0 41.0 33.0 41.0 38-39 37.793251544794956 40.0 37.0 41.0 33.0 41.0 40-41 37.68724682745033 40.0 37.0 41.0 33.0 41.0 42-43 37.58269563479993 40.0 37.0 41.0 32.5 41.0 44-45 37.44437255050782 40.0 37.0 41.0 32.0 41.0 46-47 37.31010771227615 40.0 36.0 41.0 31.5 41.0 48-49 37.063108205443584 40.0 36.0 41.0 31.0 41.0 50-51 35.72080480786521 38.0 34.0 40.0 29.0 40.5 52-53 35.92650110669189 38.5 35.0 39.5 30.0 40.0 54-55 34.00935252567859 38.0 32.5 40.0 18.0 41.0 56-57 35.400375135016645 38.0 34.5 40.0 26.0 41.0 58-59 35.60680130536525 38.5 35.0 40.0 27.0 41.0 60-61 35.47862368512416 38.0 35.0 40.0 27.0 41.0 62-63 35.15432722164488 37.5 34.5 40.0 26.0 41.0 64-65 34.8281791544881 37.0 34.0 40.0 26.0 41.0 66-67 34.49826239552446 36.5 34.0 39.5 26.0 41.0 68-69 34.05784544762761 36.0 34.0 39.0 25.5 41.0 70-71 33.7334174573366 35.0 33.0 38.5 25.0 40.0 72-73 33.37975208892961 35.0 33.0 37.5 25.0 40.0 74-75 32.96655143929223 35.0 33.0 37.0 24.0 39.0 76-77 32.60725189743931 35.0 33.0 36.5 23.5 39.0 78-79 32.283980613161816 35.0 32.0 36.0 23.0 38.5 80-81 31.900245072713428 35.0 32.0 35.5 21.5 37.0 82-83 31.443678408344258 35.0 31.0 35.0 20.0 37.0 84-85 31.27713999852852 34.5 31.0 35.0 20.0 36.0 86-87 31.080860489408522 34.0 31.0 35.0 19.5 36.0 88-89 30.88334848014818 34.0 31.0 35.0 19.0 36.0 90-91 30.529127842178056 34.0 30.0 35.0 18.0 35.0 92-93 30.39488501806634 34.0 30.0 35.0 18.0 35.0 94-95 30.279888567174854 34.0 30.0 35.0 17.5 35.0 96-97 30.105040059538084 34.0 30.0 35.0 17.0 35.0 98-99 29.91930954467849 34.0 29.0 35.0 16.5 35.0 100 29.752044622411933 34.0 29.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2568736515243373 1101 2 0.24273798537358005 1101 3 0.2544977601634386 1101 4 0.25320338948944965 1101 5 0.275803691789946 1101 6 0.30671212085778876 1101 7 0.30921292851903814 1101 8 0.31004777339247624 1101 9 0.34651165927014915 1101 10-11 0.36672670705716115 1101 12-13 0.33561567696638406 1101 14-15 0.4127077633154599 1101 16-17 0.44195524442665146 1101 18-19 0.44485453111634854 1101 20-21 0.423077470496537 1101 22-23 0.3755578361389027 1101 24-25 0.3594903680488457 1101 26-27 0.3847664786466183 1101 28-29 0.4126995936709008 1101 30-31 0.3801950448268343 1101 32-33 0.4839925530593874 1101 34-35 0.5362648797680123 1101 36-37 0.4407769952113938 1101 38-39 0.38424931293096876 1101 40-41 0.42840677061072086 1101 42-43 0.4342719161125146 1101 44-45 0.4389431070185523 1101 46-47 0.43158724759670264 1101 48-49 0.5032905117756954 1101 50-51 0.3725152616108076 1101 52-53 0.5108425486924588 1101 54-55 2.7673059298484404 1101 56-57 1.1728711179654567 1101 58-59 0.7642269699276696 1101 60-61 0.6747556943871587 1101 62-63 0.688049031533879 1101 64-65 0.6654200713336209 1101 66-67 0.6748651057638639 1101 68-69 0.7736069043941782 1101 70-71 0.6865318911066467 1101 72-73 0.6183715135256236 1101 74-75 0.7382961613527286 1101 76-77 0.7236814896777446 1101 78-79 0.6417475875337431 1101 80-81 0.6339609439089351 1101 82-83 0.8152427923476324 1101 84-85 0.6741557531236282 1101 86-87 0.670132547923739 1101 88-89 0.6537933025816649 1101 90-91 0.9210775854100781 1101 92-93 0.8594039001640432 1101 94-95 0.6722020659950196 1101 96-97 0.6240331203038174 1101 98-99 0.6404539761035011 1101 100 0.6983952161784401 1102 1 0.12945869886745953 1102 2 0.13993228011319303 1102 3 0.17389953319304396 1102 4 0.20212439573931817 1102 5 0.2234897834063858 1102 6 0.2987656054456096 1102 7 0.2505409258923734 1102 8 0.21355433378082012 1102 9 0.27735608694933234 1102 10-11 0.2810056439305626 1102 12-13 0.2990424229846198 1102 14-15 0.2923565180607923 1102 16-17 0.3131200907721592 1102 18-19 0.2970085605501964 1102 20-21 0.3570855504389314 1102 22-23 0.3083764458754459 1102 24-25 0.20780360842478984 1102 26-27 0.19527462791754147 1102 28-29 0.17551990232249182 1102 30-31 0.12460114135108569 1102 32-33 0.2196889889015452 1102 34-35 0.3349417834026909 1102 36-37 0.2455994120910745 1102 38-39 0.16393040721373353 1102 40-41 0.10296213662936538 1102 42-43 0.1069056359282996 1102 44-45 0.10445937052919874 1102 46-47 0.0664325896513418 1102 48-49 0.11831529576470246 1102 50-51 0.004640331047660595 1102 52-53 0.16562210268286037 1102 54-55 2.0006360599822557 1102 56-57 0.791276302590461 1102 58-59 0.563493112365741 1102 60-61 0.541676691797015 1102 62-63 0.5648050373810847 1102 64-65 0.5386293250108309 1102 66-67 0.5625446399870029 1102 68-69 0.6234215886955141 1102 70-71 0.6337460024854096 1102 72-73 0.5997121883617638 1102 74-75 0.6904233621637133 1102 76-77 0.6642213980810681 1102 78-79 0.6103439106987167 1102 80-81 0.6334772403201292 1102 82-83 0.7165013630959933 1102 84-85 0.6025602674819517 1102 86-87 0.5361204292190287 1102 88-89 0.5609260082210987 1102 90-91 0.7676642287950664 1102 92-93 0.670333154713223 1102 94-95 0.5825365138275735 1102 96-97 0.49722326899000535 1102 98-99 0.5506482406338264 1102 100 0.5798849786352243 1103 1 0.20220086651060143 1103 2 0.21141619610813933 1103 3 0.18626126407890098 1103 4 0.16006148729696434 1103 5 0.1971593374957763 1103 6 0.2000063254961688 1103 7 0.22158547438973386 1103 8 0.18983141801222558 1103 9 0.26351689108534515 1103 10-11 0.24438254953195582 1103 12-13 0.21190531448233685 1103 14-15 0.2623535865177118 1103 16-17 0.26985908297119465 1103 18-19 0.27880944730753754 1103 20-21 0.28582929281451186 1103 22-23 0.31448378348353856 1103 24-25 0.29132737876737735 1103 26-27 0.25568377960477306 1103 28-29 0.22405233936932234 1103 30-31 0.1953971192093178 1103 32-33 0.3412068133488617 1103 34-35 0.4156613572607597 1103 36-37 0.3412747430090093 1103 38-39 0.26997884463752797 1103 40-41 0.2927754924650756 1103 42-43 0.20749157394291728 1103 44-45 0.2644309497835948 1103 46-47 0.2116681703917962 1103 48-49 0.31259403990037526 1103 50-51 0.28146601658421844 1103 52-53 0.37103579494078787 1103 54-55 2.6851935665420683 1103 56-57 1.1568964875293943 1103 58-59 0.7130279916258004 1103 60-61 0.5936904875680682 1103 62-63 0.611205325506269 1103 64-65 0.5813821795643079 1103 66-67 0.5521675596762421 1103 68-69 0.7095409422135859 1103 70-71 0.6842658895243048 1103 72-73 0.6423200957680208 1103 74-75 0.7282476755651928 1103 76-77 0.6091840727006854 1103 78-79 0.6862975578065615 1103 80-81 0.6843236091969764 1103 82-83 0.8040509771038487 1103 84-85 0.6953657838603817 1103 86-87 0.594182149320158 1103 88-89 0.5859367719395188 1103 90-91 0.8121014197983847 1103 92-93 0.7503729023060579 1103 94-95 0.6103432805259708 1103 96-97 0.5829455038405769 1103 98-99 0.5803138039386369 1103 100 0.6382899415690524 1104 1 0.12443241684895412 1104 2 0.11211243123687353 1104 3 0.12193985246170769 1104 4 0.1216935500734877 1104 5 0.12526515694826656 1104 6 0.18679717513759897 1104 7 0.11466345701549585 1104 8 0.11327137655485586 1104 9 0.14905321410177663 1104 10-11 0.17421019448575947 1104 12-13 0.15174811525465515 1104 14-15 0.15871512720170955 1104 16-17 0.19713096377982708 1104 18-19 0.2212977100525535 1104 20-21 0.19276094981566416 1104 22-23 0.15508912594872726 1104 24-25 0.1740239175335816 1104 26-27 0.0858993548149769 1104 28-29 0.1293286602957764 1104 30-31 0.07227620549360125 1104 32-33 0.21267838320401466 1104 34-35 0.28009631726897055 1104 36-37 0.2191919219102516 1104 38-39 0.13907449811427597 1104 40-41 0.1478429148308109 1104 42-43 0.15680272191814026 1104 44-45 0.13315394466899733 1104 46-47 0.17922887360197848 1104 48-49 0.2768940390783783 1104 50-51 0.18603905095265816 1104 52-53 0.2332610977637657 1104 54-55 0.9281734080123059 1104 56-57 0.3760624811381419 1104 58-59 0.1999789966370784 1104 60-61 0.18497536969187678 1104 62-63 0.17394307115845464 1104 64-65 0.17752092603114278 1104 66-67 0.108375564410224 1104 68-69 0.24842311520131943 1104 70-71 0.17709529003605695 1104 72-73 0.0909940947592176 1104 74-75 0.20133334209738507 1104 76-77 0.19065024248822482 1104 78-79 0.14321059650714574 1104 80-81 0.14469196552611052 1104 82-83 0.2537539627912935 1104 84-85 0.1768377826730685 1104 86-87 0.10776223368776172 1104 88-89 0.05670115131198017 1104 90-91 0.28295791267473547 1104 92-93 0.21633560691652676 1104 94-95 0.1200767455290297 1104 96-97 -0.02276044717503467 1104 98-99 -0.0046355668521442794 1104 100 -0.002336327551702766 1105 1 0.12165932162616144 1105 2 0.12233217736167745 1105 3 0.133945681694577 1105 4 0.11309014462207045 1105 5 0.11435020155521869 1105 6 0.1685352879039641 1105 7 0.13034659310053343 1105 8 0.17283890425458992 1105 9 0.18761535665546347 1105 10-11 0.15579557277329315 1105 12-13 0.13948257652153728 1105 14-15 0.1755449509114939 1105 16-17 0.16269204989069408 1105 18-19 0.19210896371635044 1105 20-21 0.19993451244130256 1105 22-23 0.1626892479630655 1105 24-25 0.1664944295544828 1105 26-27 0.1362473473214223 1105 28-29 0.1503092644428321 1105 30-31 0.15606639096884578 1105 32-33 0.26991780950309163 1105 34-35 0.3068715121180148 1105 36-37 0.24486417724656206 1105 38-39 0.20049580122221045 1105 40-41 0.2172317509105639 1105 42-43 0.12618641464041502 1105 44-45 0.18775410789642422 1105 46-47 0.17486118998932199 1105 48-49 0.2748839797935929 1105 50-51 0.09937255458350336 1105 52-53 0.2663707522931631 1105 54-55 2.127520246480394 1105 56-57 0.8316567029676492 1105 58-59 0.4271390425417749 1105 60-61 0.3175157734037981 1105 62-63 0.30317924229566273 1105 64-65 0.30297259274506416 1105 66-67 0.3482201027557892 1105 68-69 0.4636065302737009 1105 70-71 0.42299566543649547 1105 72-73 0.3330238917908659 1105 74-75 0.42435420474359375 1105 76-77 0.38639605586288894 1105 78-79 0.3331730024521846 1105 80-81 0.3712796405985195 1105 82-83 0.38947152919836014 1105 84-85 0.35213115932384653 1105 86-87 0.3723116344205657 1105 88-89 0.3265195715367888 1105 90-91 0.4809419143722842 1105 92-93 0.4596232452498761 1105 94-95 0.33810349514865834 1105 96-97 0.2268025893877912 1105 98-99 0.2427924634665004 1105 100 0.28834773220456356 1106 1 0.05282425477039254 1106 2 0.071448298559325 1106 3 0.05146503324630913 1106 4 0.04813731851596259 1106 5 0.06313250776690893 1106 6 0.14598774577163454 1106 7 0.1133159715987091 1106 8 0.11768002203067596 1106 9 0.13057476296372528 1106 10-11 0.06822423499716734 1106 12-13 0.08465619889321374 1106 14-15 0.10461158272651261 1106 16-17 0.12725124302576774 1106 18-19 0.14749791163573178 1106 20-21 0.12903574719550193 1106 22-23 0.13377718643223346 1106 24-25 0.14761561747214813 1106 26-27 0.11613433269069162 1106 28-29 0.10562815277049964 1106 30-31 0.08830400557154405 1106 32-33 0.1885726713857636 1106 34-35 0.26620106172256897 1106 36-37 0.17223352183067675 1106 38-39 0.13433689796388393 1106 40-41 0.08514305708854408 1106 42-43 0.15146087286233012 1106 44-45 0.11232347772637041 1106 46-47 0.16448853750963366 1106 48-49 0.207339371958696 1106 50-51 0.22324926831451108 1106 52-53 0.21801404110353673 1106 54-55 2.415240804262872 1106 56-57 0.9129075088832792 1106 58-59 0.3890044315101022 1106 60-61 0.16675955729476755 1106 62-63 0.2254655599558859 1106 64-65 0.23676116765521016 1106 66-67 0.18514078031396508 1106 68-69 0.2885970459329741 1106 70-71 0.25735228508167296 1106 72-73 0.19180334883790096 1106 74-75 0.2863754846105664 1106 76-77 0.29214342956910144 1106 78-79 0.22565336787430823 1106 80-81 0.28334876510658447 1106 82-83 0.3938053979449627 1106 84-85 0.2937508065110066 1106 86-87 0.19495044810985007 1106 88-89 0.22441235410026295 1106 90-91 0.41654076942350926 1106 92-93 0.299672050662128 1106 94-95 0.19909983788835817 1106 96-97 0.08539421188852714 1106 98-99 0.12757612530002405 1106 100 0.2970440358668007 1107 1 0.015224978277863954 1107 2 0.02358546397500305 1107 3 0.0483982087218493 1107 4 0.012764100929253175 1107 5 0.05082762079216252 1107 6 0.11083220662169424 1107 7 0.10443355081928019 1107 8 0.09469321848906986 1107 9 0.10709112245000085 1107 10-11 0.06298600751831174 1107 12-13 0.08477543234092622 1107 14-15 0.09293813324530475 1107 16-17 0.07147944026165476 1107 18-19 0.12354051735476901 1107 20-21 0.12706583792979131 1107 22-23 0.1250894158791951 1107 24-25 0.04997466360212144 1107 26-27 0.09110000761682358 1107 28-29 0.09096906782020397 1107 30-31 0.039845319497615606 1107 32-33 0.14417609605839488 1107 34-35 0.1975819373254737 1107 36-37 0.10921642454834313 1107 38-39 0.09568699656119861 1107 40-41 0.1415954517011997 1107 42-43 0.10792140081322543 1107 44-45 -0.012787516337603222 1107 46-47 0.0350597769364569 1107 48-49 0.04934715492453989 1107 50-51 -0.005820910071982155 1107 52-53 0.11615664930318559 1107 54-55 2.302141635759689 1107 56-57 0.7585614764872375 1107 58-59 0.3205361919091487 1107 60-61 0.1727273614331466 1107 62-63 0.1431723045706903 1107 64-65 0.18736178182974328 1107 66-67 0.07867798197537468 1107 68-69 0.20246776220282925 1107 70-71 0.2194554609650794 1107 72-73 0.11968871964372596 1107 74-75 0.19229126775852734 1107 76-77 0.13953650121653993 1107 78-79 0.0525565060605544 1107 80-81 0.15617035705970395 1107 82-83 0.1970582907097409 1107 84-85 0.15481377135063212 1107 86-87 0.0936392550061278 1107 88-89 0.06538619265936418 1107 90-91 0.27989777013032224 1107 92-93 0.12862206949347765 1107 94-95 0.039064132967535414 1107 96-97 -0.008452741508776285 1107 98-99 0.05139238120573708 1107 100 0.05480842993672752 1108 1 -0.06269000882164733 1108 2 -0.02554417050424007 1108 3 -0.03341958524497102 1108 4 0.01701246788562827 1108 5 -0.029346724963616566 1108 6 0.010263036470497866 1108 7 0.017370222639442545 1108 8 0.010881470670874194 1108 9 0.020921751261283816 1108 10-11 -0.05189101164472021 1108 12-13 -0.023385793857691795 1108 14-15 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-1.653499081890537 2316 100 -1.780522298637937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 370.0 3 3308.0 4 1180.0 5 2223.0 6 2205.0 7 9906.0 8 30317.0 9 38967.0 10 40640.0 11 42564.0 12 47983.0 13 54672.0 14 64150.0 15 76924.0 16 97261.0 17 120169.0 18 133622.0 19 151248.0 20 174591.0 21 208625.0 22 262526.0 23 366091.0 24 502926.0 25 615184.0 26 638876.0 27 612746.0 28 608459.0 29 660272.0 30 770533.0 31 935014.0 32 1168413.0 33 1524387.0 34 2152734.0 35 3367057.0 36 6023119.0 37 1.0535481E7 38 9486461.0 39 1486312.0 40 329.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.506289493860216 28.810317420096343 27.378577084250622 26.304816001792812 2 23.56322823702582 30.497373822421835 25.498741278183505 20.440656662368838 3 24.870193389533274 24.11740338427034 26.715186071639113 24.29721715455727 4 20.635370357705465 27.279359217914156 23.396200195073273 28.689070229307102 5 24.15334622516427 28.76231797708366 25.886025505554155 21.198310292197917 6 28.71746403193591 27.45189776704093 23.95333523422526 19.877302966797895 7 23.53593564635064 24.710310239659318 28.85039826980521 22.903355844184826 8 26.40689038744678 26.32515931990248 24.63313605216765 22.634814240483088 9 25.233173651823137 23.995408493027824 27.26238650349523 23.50903135165381 10-11 23.098235133506215 27.453355317992482 26.642834234167402 22.8055753143339 12-13 22.853872079860636 25.936718330976287 26.67126140906932 24.538148180093753 14-15 22.83692592712458 29.053306826788443 25.26794841462925 22.84181883145773 16-17 24.636273018365724 25.511697010337308 25.33306204264381 24.51896792865315 18-19 22.848396460547697 27.186074126835507 25.450499437836633 24.515029974780163 20-21 24.50113098925438 27.40118219429819 25.382621795900533 22.71506502054689 22-23 24.58011866498629 25.73539667518584 26.85104598051683 22.833438679311037 24-25 22.993008016389943 27.106318684886226 25.214790685463694 24.685882613260137 26-27 23.015126586430014 25.568645895825785 28.581888364277503 22.834339153466697 28-29 23.065002800434122 25.59940352898299 28.520261027224556 22.815332643358328 30-31 22.932385931773805 25.45344575097177 28.660214204167712 22.95395411308671 32-33 23.138137731389573 25.656776845562092 28.391695605683577 22.813389817364758 34-35 23.160622135160878 25.802836283745574 28.221498803836653 22.815042777256895 36-37 22.941265646271326 25.758767112899424 28.426054747278705 22.873912493550545 38-39 23.016196147097787 25.728432067619828 28.477268392784776 22.778103392497613 40-41 23.0899807615462 25.752856080820763 28.37407738704205 22.783085770590986 42-43 23.09355555039235 25.764827613137705 28.390514590262526 22.75110224620742 44-45 23.238054698501035 25.873643401242536 28.282697268019557 22.605604632236872 46-47 23.199202916205607 25.963785834027707 28.198228948190135 22.638782301576548 48-49 23.305172047018505 25.947938672244465 28.082991370038922 22.663897910698108 50-51 23.396147261102318 26.027992505178016 28.018826688800225 22.55703354491944 52-53 23.48489782377896 26.14039876542844 27.84569448487532 22.529008925917278 54-55 24.363622389504375 26.445555167132706 27.191101852009574 21.99972059135334 56-57 24.0589497593324 26.649521440525458 26.771953459788676 22.519575340353466 58-59 24.268186434562292 26.894708812703243 26.366653944736086 22.470450807998375 60-61 24.378042353157607 27.066543591984317 26.204649954579416 22.35076410027866 62-63 24.511368194866645 27.248499042626374 26.054804775693285 22.185327986813697 64-65 24.676295502563388 27.313846404465174 25.96497761952903 22.04488047344241 66-67 24.772393396323565 27.227147018478824 26.068833000729093 21.931626584468518 68-69 24.9498149870276 27.149251240304196 26.166644850635073 21.734288922033134 70-71 25.08842233593394 27.02807271658292 26.21769414214089 21.66581080534225 72-73 25.2291108117984 26.963143790888516 26.255512502557167 21.552232894755925 74-75 25.47498191645986 26.979583625034596 26.18085517119472 21.36457928731082 76-77 25.706678858526644 27.025153955546806 25.978857295225495 21.28930989070106 78-79 25.9025551126933 27.03217808091229 25.81666832686237 21.248598479532042 80-81 26.183774306377007 27.091894385628734 25.592575804752578 21.13175550324168 82-83 26.45285685927707 27.071987140454283 25.39707140320337 21.07808459706527 84-85 26.48996444410893 27.082857596222503 25.36369455306503 21.063483406603535 86-87 26.675627904643367 27.062384373475258 25.29502131010363 20.96696641177775 88-89 26.813420248860638 27.040802407665822 25.198636546640685 20.94714079683286 90-91 26.998725502971045 26.962584421109774 25.146775901627134 20.891914174292047 92-93 26.974000789218998 27.0210055219397 25.22203136708846 20.782962321752837 94-95 27.03848515819663 27.08259873691085 25.12569433679796 20.753221768094562 96-97 27.032910689956243 27.14717348519841 25.10307688657984 20.716838938265507 98-99 27.108333366379693 27.27602436753828 25.045078629389177 20.57056363669285 100 27.07931014826748 27.413533697843445 24.894458873095683 20.612697280793387 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13287.0 1 11952.0 2 9337.5 3 7782.5 4 7368.5 5 7189.5 6 7355.5 7 7721.0 8 7895.5 9 8123.5 10 8624.0 11 11602.5 12 77384.0 13 102199.5 14 60884.5 15 61772.5 16 69745.5 17 77588.5 18 85187.0 19 92925.5 20 102229.0 21 116027.5 22 134848.5 23 156434.5 24 180275.5 25 208756.0 26 241432.0 27 279890.0 28 327750.5 29 377919.5 30 423063.5 31 472902.5 32 527565.5 33 575311.0 34 622551.0 35 679120.5 36 743507.5 37 804183.0 38 874054.5 39 948659.5 40 1005682.5 41 1072508.5 42 1151741.5 43 1207025.0 44 1253742.0 45 1323925.5 46 1408011.0 47 1488699.0 48 1569413.5 49 1638825.5 50 1687927.5 51 1731227.0 52 1758003.5 53 1755530.5 54 1726032.0 55 1673380.0 56 1594979.0 57 1482852.0 58 1334487.5 59 1155342.5 60 963127.0 61 786393.0 62 632361.0 63 491774.5 64 375837.5 65 286330.5 66 213629.0 67 159317.0 68 121352.5 69 95496.5 70 77615.5 71 64553.5 72 53539.0 73 41519.5 74 30481.5 75 22972.0 76 17345.0 77 12120.5 78 8336.0 79 5769.0 80 3801.0 81 2538.5 82 1719.0 83 1185.0 84 826.0 85 603.5 86 486.5 87 383.5 88 267.5 89 216.0 90 189.0 91 163.0 92 129.5 93 96.5 94 78.5 95 62.0 96 47.0 97 33.5 98 24.0 99 15.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003870486771245747 2 0.0 3 1.1623083397134375E-5 4 3.789125187465806E-4 5 0.0021851396786612626 6 0.006857619204309282 7 0.007055211622060566 8 0.008373269279295603 9 0.010497968924291768 10-11 0.009092738141578223 12-13 0.01142549097938309 14-15 0.015298302367308265 16-17 0.02005795501843479 18-19 0.021943219145449985 20-21 0.024090002648900707 22-23 0.0258973921171551 24-25 0.022131513096483563 26-27 0.025533589606824794 28-29 0.030000340556343534 30-31 0.0395208081669363 32-33 0.028313831155419335 34-35 0.03181237925795678 36-37 0.03753326090602632 38-39 0.045763566259537175 40-41 0.0455322668999342 42-43 0.049574775305457536 44-45 0.055827994173115826 46-47 0.05490744596806278 48-49 0.05086377525419974 50-51 0.048103292947380326 52-53 0.040686603431668876 54-55 0.0379203095831509 56-57 0.02585322440024599 58-59 0.021933920678732278 60-61 0.0228091388585365 62-63 0.03496572178359934 64-65 0.03865256383717037 66-67 0.052425917662774595 68-69 0.05100441456330506 70-71 0.04701537234140855 72-73 0.05444368494051712 74-75 0.05333832970944965 76-77 0.04338548339648348 78-79 0.03693002287771505 80-81 0.03985322835209435 82-83 0.038674647695624925 84-85 0.04366095047299556 86-87 0.050968383004773944 88-89 0.04846360853269149 90-91 0.05219345599483191 92-93 0.051547212557951236 94-95 0.04853799626643315 96-97 0.05064177436131447 98-99 0.05275368861457379 100 0.045743807017762046 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.3017845E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.0117927109502 #Duplication Level Percentage of deduplicated Percentage of total 1 66.42638252023598 25.91412265417732 2 15.536854490639197 12.122410935380243 3 6.502805991843738 7.610583581799984 4 3.339484354600633 5.211170856125649 5 1.9631706033993939 3.82934023180241 6 1.2634749747955822 2.9574254287318977 7 0.8774200764080394 2.3960811098879566 8 0.631382984560972 1.970510569193097 9 0.4782960856160497 1.6793268971861002 >10 2.6538925176040182 18.594952370879422 >50 0.17976321203494436 4.962310902364685 >100 0.14554029213337152 9.078544178205659 >500 9.80634082937658E-4 0.2561767082051333 >1k 4.916750890372219E-4 0.3357568242190128 >5k 3.57521903103712E-5 0.0893899589074432 >10k+ 2.3834765644389674E-5 2.991896792934221 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1193650 2.7747786993978893 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 46961 0.10916632388256549 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0800272538059496 0.0 0.0 2.324616679426875E-6 0.0 2 0.0800365522726673 1.394770007656125E-5 0.0 2.324616679426875E-6 2.324616679426875E-6 3 0.08003887688934673 2.0921550114841875E-5 0.0 2.324616679426875E-6 6.973850038280625E-6 4 0.08003887688934673 2.5570783473695626E-5 0.0 4.64923335885375E-6 1.1623083397134375E-5 5 0.08004585073938501 3.0220016832549374E-5 0.0 4.64923335885375E-6 1.6272316755988124E-5 6 0.08005514920610272 3.254463351197625E-5 0.0 9.2984667177075E-6 2.324616679426875E-5 7 0.08005514920610272 3.719386687083E-5 0.0 1.1623083397134375E-5 2.324616679426875E-5 8 0.08005747382278215 4.416771690911063E-5 0.0 1.1623083397134375E-5 2.324616679426875E-5 9 0.08006677228949986 4.64923335885375E-5 0.0 1.6272316755988124E-5 2.78954001531225E-5 10-11 0.08009931692301184 5.4628491966531565E-5 0.0 1.8596933435415E-5 3.138232517226281E-5 12-13 0.08017951619845207 7.206311706223312E-5 0.0 2.5570783473695626E-5 4.416771690911062E-5 14-15 0.08032712935759567 8.36862004593675E-5 0.0 2.5570783473695626E-5 5.927772532538531E-5 16-17 0.08052472177534695 8.71731254785078E-5 0.0 2.9057708492835935E-5 7.206311706223312E-5 18-19 0.08071185341804081 9.414697551678845E-5 0.0 3.0220016832549374E-5 8.833543381822124E-5 20-21 0.08096988586945719 1.0344544223449594E-4 0.0 3.254463351197625E-5 9.530928385650188E-5 22-23 0.08143132228032343 1.1623083397134374E-4 0.0 3.719386687083E-5 1.1041929227277657E-4 24-25 0.08280749535454415 1.208800673301975E-4 0.0 3.835617521054344E-5 1.2088006733019751E-4 26-27 0.08711733467820157 1.2785391736847812E-4 0.0 4.184310022968375E-5 1.3017853404790502E-4 28-29 0.09921696449461845 1.3366545906704532E-4 0.0 4.184310022968375E-5 1.3831469242589907E-4 30-31 0.12665092823687474 1.394770007656125E-4 0.0 4.8816950267964375E-5 1.5807393420102752E-4 32-33 0.18081798379254005 1.4412623412446624E-4 0.0 4.8816950267964375E-5 1.67372400918735E-4 34-35 0.27315175829937555 1.4877546748332E-4 0.0 4.8816950267964375E-5 1.7202163427758874E-4 36-37 0.41224519731288256 1.5574931752160065E-4 0.0 4.8816950267964375E-5 1.7899548431586938E-4 38-39 0.6005414729631389 1.6272316755988126E-4 0.0 5.114156694739125E-5 1.8596933435415E-4 40-41 0.8425119854330221 1.6504778423930813E-4 0.0 5.346618362681812E-5 1.9061856771300376E-4 42-43 1.1360413335442536 1.8015779265558282E-4 0.0 5.927772532538531E-5 2.0340395944985155E-4 44-45 1.4711731840588482 1.8364471767472312E-4 0.0 6.50892670239525E-5 2.2316320122498E-4 46-47 1.8573594283953554 1.917808760527172E-4 2.324616679426875E-6 6.973850038280625E-5 2.2897474292354718E-4 48-49 2.29847636486672 1.952678010718575E-4 2.324616679426875E-6 7.206311706223312E-5 2.382732096412547E-4 50-51 2.7823116197475724 2.0572857612927842E-4 2.324616679426875E-6 7.438773374166E-5 2.440847513398219E-4 52-53 3.3168339790149877 2.1154011782784562E-4 2.324616679426875E-6 7.438773374166E-5 2.510586013781025E-4 54-55 3.899080718711037 2.1502704284698596E-4 2.324616679426875E-6 7.438773374166E-5 2.661686097943772E-4 56-57 4.54848447196739 2.196762762058397E-4 2.324616679426875E-6 7.903696710051375E-5 2.7662938485179813E-4 58-59 5.240358971956871 2.2781243458383374E-4 2.324616679426875E-6 7.903696710051375E-5 2.824409265503653E-4 60-61 5.969540082726134 2.324616679426875E-4 2.324616679426875E-6 8.136158377994063E-5 2.882524682489325E-4 62-63 6.7274174240945825 2.382732096412547E-4 2.324616679426875E-6 8.252389211965407E-5 3.0452478500492063E-4 64-65 7.506977627540385 2.4059782632068156E-4 2.324616679426875E-6 8.71731254785078E-5 3.184724850814819E-4 66-67 8.31138449636424 2.545455263972428E-4 2.324616679426875E-6 9.066005049764813E-5 3.3358249349775657E-4 68-69 9.143797184633492 2.5919475975609655E-4 2.324616679426875E-6 9.414697551678845E-5 3.498548102537447E-4 70-71 9.996165544787285 2.615193764355234E-4 2.324616679426875E-6 9.530928385650188E-5 3.661271270097328E-4 72-73 10.860430130798044 2.684932264738041E-4 2.324616679426875E-6 9.879620887564219E-5 3.765879020671538E-4 74-75 11.736861760508923 2.7430476817237126E-4 2.324616679426875E-6 9.995851721535562E-5 3.835617521054344E-4 76-77 12.630306097388189 2.894147765886459E-4 2.324616679426875E-6 1.0344544223449594E-4 3.928602188231419E-4 78-79 13.531232213050188 3.033624766652072E-4 2.324616679426875E-6 1.0577005891392281E-4 4.056456105599897E-4 80-81 14.44124176838705 3.2312171844033565E-4 2.324616679426875E-6 1.0693236725363625E-4 4.1261946059827033E-4 82-83 15.357913675127147 3.3009556847861626E-4 2.324616679426875E-6 1.150685256316303E-4 4.2656716067483155E-4 84-85 16.28887500059568 3.440432685551775E-4 2.324616679426875E-6 1.1623083397134376E-4 4.3470331905282565E-4 86-87 17.22525849446898 3.707763603685866E-4 2.324616679426875E-6 1.1623083397134376E-4 4.4516409411024657E-4 88 17.931005144492943 3.8588636878486126E-4 2.324616679426875E-6 1.1623083397134376E-4 4.5097563580881374E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 169410 0.0 78.45895 8 GTGGTAT 170105 0.0 78.08622 6 AGTGGTA 171810 0.0 77.37349 5 GTATCAA 171520 0.0 77.291046 9 TGGTATC 172165 0.0 77.020905 7 AAGCAGT 181510 0.0 74.25803 1 CAGTGGT 189685 0.0 70.37446 4 GCAGTGG 191360 0.0 70.37227 3 AGCAGTG 201175 0.0 67.2194 2 CAACGCA 166125 0.0 42.676952 12-13 ATCAACG 169025 0.0 42.05491 10-11 ACGCAGA 172100 0.0 41.132893 14-15 AGAGTAC 169575 0.0 40.96678 18-19 TCAACGC 165210 0.0 40.1971 12-13 GAGTACT 159425 0.0 39.46462 20-21 AGTACTT 170165 0.0 39.423256 20-21 AACGCAG 168625 0.0 39.25654 14-15 TATCAAC 175180 0.0 37.860165 10-11 CGCAGAG 177145 0.0 37.226612 16-17 GTACTTT 168905 0.0 37.178665 22-23 >>END_MODULE