FastQCFastQC Report
Thu 26 May 2016
SRR1660652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43017845
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12457112.895800568345532No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT507260.11791850568060767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1737100.078.317998
GTGGTAT1752050.077.845026
AGTGGTA1755450.077.4962845
TGGTATC1767600.077.019397
GTATCAA1767550.076.906569
AAGCAGT1850600.074.2285461
CAGTGGT1930050.070.789824
GCAGTGG1940650.070.594323
AGCAGTG2048200.067.1399842
CAACGCA1695100.042.75916312-13
ATCAACG1723750.042.18737810-11
ACGCAGA1753800.041.3159114-15
AGAGTAC1751350.040.85294318-19
TCAACGC1687500.040.1336612-13
AGTACTT1743350.039.69576620-21
GAGTACT1638150.039.57420720-21
AACGCAG1718700.039.31213814-15
TATCAAC1797750.037.79913710-11
GTACTTT1731250.037.36458622-23
CGCAGAG1806350.037.31271416-17
Produced by FastQC (version 0.11.5)