##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660652_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43017845 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64457589635185 34.0 31.0 34.0 31.0 34.0 2 32.8553448923348 34.0 31.0 34.0 31.0 34.0 3 32.91753199166532 34.0 31.0 34.0 31.0 34.0 4 36.34411240265522 37.0 37.0 37.0 35.0 37.0 5 36.20053877640779 37.0 37.0 37.0 35.0 37.0 6 36.232032171765 37.0 37.0 37.0 35.0 37.0 7 36.181512486271686 37.0 37.0 37.0 35.0 37.0 8 36.12442587488983 37.0 37.0 37.0 35.0 37.0 9 37.9305000052885 39.0 38.0 39.0 35.0 39.0 10-11 37.88787306756068 39.0 38.0 39.0 35.0 39.0 12-13 37.932631527683455 39.0 38.0 39.0 35.0 39.0 14-15 39.38385174803619 41.0 39.0 41.0 36.0 41.0 16-17 39.312213966087796 41.0 39.0 41.0 36.0 41.0 18-19 39.28505009955752 40.5 39.0 41.0 36.0 41.0 20-21 39.19895591004152 40.0 39.0 41.0 36.0 41.0 22-23 38.99754311263152 40.0 38.5 41.0 35.5 41.0 24-25 38.98375782189926 40.0 39.0 41.0 35.0 41.0 26-27 38.9125906004822 40.0 38.0 41.0 35.0 41.0 28-29 38.79446013625276 40.0 38.0 41.0 35.0 41.0 30-31 38.8625716606678 40.0 38.0 41.0 35.0 41.0 32-33 38.79013486147434 40.0 38.0 41.0 35.0 41.0 34-35 38.702743280143395 40.0 38.0 41.0 35.0 41.0 36-37 38.55923625416382 40.0 38.0 41.0 34.5 41.0 38-39 38.44962113978513 40.0 38.0 41.0 35.0 41.0 40-41 38.39786617623454 40.0 38.0 41.0 34.0 41.0 42-43 38.3113673755624 40.0 38.0 41.0 34.0 41.0 44-45 38.21438872635298 40.0 37.5 41.0 34.0 41.0 46-47 38.090352387480124 40.0 37.0 41.0 33.5 41.0 48-49 37.8707403636793 40.0 37.0 41.0 33.0 41.0 50-51 37.7167510831842 40.0 36.5 41.0 33.0 41.0 52-53 37.44709940723437 40.0 36.0 41.0 33.0 41.0 54-55 37.012164928298944 39.0 35.5 41.0 31.5 41.0 56-57 36.60235217501016 39.0 35.0 41.0 31.0 41.0 58-59 36.18863386810753 38.5 35.0 40.0 29.5 41.0 60-61 35.93809692233537 38.0 35.0 40.0 29.5 41.0 62-63 35.67960251146937 37.5 35.0 40.0 29.0 41.0 64-65 35.39262815234004 37.0 35.0 40.0 29.0 41.0 66-67 35.00311195272566 36.5 34.0 39.0 29.0 41.0 68-69 34.68831424493719 36.0 34.0 39.0 28.5 40.5 70-71 34.35171556129788 35.0 34.0 38.5 28.0 40.0 72-73 33.998728504414856 35.0 34.0 37.5 27.5 40.0 74-75 33.58980574224488 35.0 33.0 37.0 27.0 39.0 76-77 32.143348231414194 34.0 31.5 35.5 25.0 38.5 78-79 32.99851450020334 35.0 33.0 36.0 26.5 38.5 80-81 33.019285415157356 35.0 33.0 36.0 27.0 37.0 82-83 32.846165573845 35.0 33.0 35.0 27.0 37.0 84-85 32.66986291386749 35.0 33.0 35.0 27.0 36.5 86-87 32.4870261864582 35.0 33.0 35.0 27.0 36.0 88-89 32.317881614013906 35.0 33.0 35.0 26.0 36.0 90-91 32.16780357082044 35.0 33.0 35.0 26.0 35.5 92-93 32.013094844709215 35.0 33.0 35.0 25.0 35.0 94-95 31.868901789478297 35.0 32.5 35.0 25.0 35.0 96-97 31.69000947165066 34.0 32.0 35.0 24.5 35.0 98-99 31.453010058965063 34.0 32.0 35.0 24.0 35.0 100 31.420456371071122 34.0 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4057396025879072 1101 2 0.37027375709372023 1101 3 0.32293236483080534 1101 4 0.20967540107290006 1101 5 0.333291411323394 1101 6 0.27656963062668183 1101 7 0.22499831178578233 1101 8 0.3290900814719535 1101 9 0.3065935400235418 1101 10-11 0.31665623022701794 1101 12-13 0.2500544076959841 1101 14-15 0.3349172893082297 1101 16-17 0.3127561290072052 1101 18-19 0.3437929951701051 1101 20-21 0.40163315316397785 1101 22-23 0.47063918166642793 1101 24-25 0.42162449309045513 1101 26-27 0.40258581402901683 1101 28-29 0.3955767625039286 1101 30-31 0.415140772899413 1101 32-33 0.45366401655147115 1101 34-35 0.37266975732821095 1101 36-37 0.3793956310736277 1101 38-39 0.40307883792686283 1101 40-41 0.4122967654539522 1101 42-43 0.4290089108598849 1101 44-45 0.47756393089177607 1101 46-47 0.4493730154606155 1101 48-49 0.5680898689637459 1101 50-51 0.5550226680841632 1101 52-53 0.4752026957317028 1101 54-55 0.47470796689870554 1101 56-57 0.5099662385357959 1101 58-59 0.5732201504004522 1101 60-61 0.5429432287479656 1101 62-63 0.5004017845431221 1101 64-65 0.5014118032542143 1101 66-67 0.5136733158332021 1101 68-69 0.48009808861390724 1101 70-71 0.4516689991946734 1101 72-73 0.3804459260784583 1101 74-75 0.42160908894798865 1101 76-77 0.5365095908041653 1101 78-79 0.5405305618028251 1101 80-81 0.48020274787185 1101 82-83 0.44943289136504916 1101 84-85 0.4619390445411611 1101 86-87 0.43280738429937315 1101 88-89 0.4664895175289203 1101 90-91 0.27195347679958104 1101 92-93 0.2685693301440537 1101 94-95 0.4012234704397173 1101 96-97 0.42270305084770854 1101 98-99 0.5250261259980391 1101 100 0.5003748503357741 1102 1 0.2423206225851402 1102 2 0.2467849954457506 1102 3 0.24415044093208849 1102 4 0.12522994816275457 1102 5 0.24584702079940257 1102 6 0.19054037072484675 1102 7 0.18853664364579004 1102 8 0.2761215591165538 1102 9 0.22812189345561507 1102 10-11 0.21435340348988063 1102 12-13 0.199903853247136 1102 14-15 0.28626659409669486 1102 16-17 0.30972490467884484 1102 18-19 0.31423886018683334 1102 20-21 0.2721692416338968 1102 22-23 0.3896703725722688 1102 24-25 0.36524571261323757 1102 26-27 0.3333607492737869 1102 28-29 0.3360144491313548 1102 30-31 0.35212865414668215 1102 32-33 0.36067356436807074 1102 34-35 0.2572904844639865 1102 36-37 0.23922728212277633 1102 38-39 0.32408766525951194 1102 40-41 0.3422202158544536 1102 42-43 0.36564756464687065 1102 44-45 0.3406035564053127 1102 46-47 0.32306201413825164 1102 48-49 0.4666364402517402 1102 50-51 0.4599671543836479 1102 52-53 0.4345316002105548 1102 54-55 0.34634534780952464 1102 56-57 0.34404107643833726 1102 58-59 0.47422741133470936 1102 60-61 0.39262227920070103 1102 62-63 0.341598411066677 1102 64-65 0.3451586428167417 1102 66-67 0.31220055413552217 1102 68-69 0.3566900792458796 1102 70-71 0.3361971243836379 1102 72-73 0.21177178742171776 1102 74-75 0.2183761939587967 1102 76-77 0.36662553654247176 1102 78-79 0.3989050744248601 1102 80-81 0.36939304728504396 1102 82-83 0.3639054242976556 1102 84-85 0.33928020219980937 1102 86-87 0.28876020646941924 1102 88-89 0.31028186937456326 1102 90-91 0.3102978295297021 1102 92-93 0.33348963360250394 1102 94-95 0.2675663145551397 1102 96-97 0.2973860780010398 1102 98-99 0.4046829325872956 1102 100 0.4016339367863182 1103 1 0.29725990067592534 1103 2 0.26090083914535 1103 3 0.21874718209721067 1103 4 0.15890618432372605 1103 5 0.25376903300354314 1103 6 0.16664920322691046 1103 7 0.1681712973520746 1103 8 0.2638352770973995 1103 9 0.2067167734823272 1103 10-11 0.1585761148194038 1103 12-13 0.16355467164446935 1103 14-15 0.18032474291430134 1103 16-17 0.24944986484214127 1103 18-19 0.2542421801573127 1103 20-21 0.30497259662084986 1103 22-23 0.3503335165290764 1103 24-25 0.3163329248623228 1103 26-27 0.2850515132991447 1103 28-29 0.3166920333277474 1103 30-31 0.32066071396909734 1103 32-33 0.31400363059995584 1103 34-35 0.2656341497232617 1103 36-37 0.2478520754130784 1103 38-39 0.29395160279010923 1103 40-41 0.3389325531572709 1103 42-43 0.3008597362352248 1103 44-45 0.29876119430117853 1103 46-47 0.2699260782478845 1103 48-49 0.39387519204004917 1103 50-51 0.3393005388577848 1103 52-53 0.28901662332114597 1103 54-55 0.2284693803333795 1103 56-57 0.25888831487594643 1103 58-59 0.34821214765239716 1103 60-61 0.36168800360034226 1103 62-63 0.3101475567568954 1103 64-65 0.2934207966862701 1103 66-67 0.3052962245740929 1103 68-69 0.2932649974571504 1103 70-71 0.2682988125426462 1103 72-73 0.19445900290799756 1103 74-75 0.2601805449183061 1103 76-77 0.3178585743238713 1103 78-79 0.41577918306558104 1103 80-81 0.3591313629503432 1103 82-83 0.3415817932510805 1103 84-85 0.3463482813936096 1103 86-87 0.32726912698016264 1103 88-89 0.3012527932853146 1103 90-91 0.2572257555877968 1103 92-93 0.2745483012493679 1103 94-95 0.2421933930245075 1103 96-97 0.26400790541459074 1103 98-99 0.4061335767881573 1103 100 0.3645920508039495 1104 1 0.239805746814433 1104 2 0.19996957801497217 1104 3 0.16961199713309583 1104 4 0.11810829990485416 1104 5 0.18805647555764438 1104 6 0.12421335025456415 1104 7 0.11414325987350082 1104 8 0.21430015172173 1104 9 0.1733484892867594 1104 10-11 0.16742313947025167 1104 12-13 0.13091039421705375 1104 14-15 0.12167038759032778 1104 16-17 0.1390597149631887 1104 18-19 0.18833078436129824 1104 20-21 0.21133526000478042 1104 22-23 0.21005388214717868 1104 24-25 0.212487527019114 1104 26-27 0.20360319467256716 1104 28-29 0.17934306080185536 1104 30-31 0.21724054578722019 1104 32-33 0.22246901445465994 1104 34-35 0.22834325230175523 1104 36-37 0.20987519968213064 1104 38-39 0.23291490504811208 1104 40-41 0.2303001190085041 1104 42-43 0.19884506732525153 1104 44-45 0.11717451511569266 1104 46-47 0.14237940583582542 1104 48-49 0.2826728742407738 1104 50-51 0.276886957299034 1104 52-53 0.24630137414212783 1104 54-55 0.16257001184376207 1104 56-57 0.14325660635322635 1104 58-59 0.2919733324830034 1104 60-61 0.2578117745132289 1104 62-63 0.1956939895459655 1104 64-65 0.11987236727605222 1104 66-67 0.22436049594949736 1104 68-69 0.24812578573015998 1104 70-71 0.26532431224569564 1104 72-73 0.12370158000242526 1104 74-75 0.11027846764775262 1104 76-77 0.28746654653557613 1104 78-79 0.3413784365760506 1104 80-81 0.23172061599958482 1104 82-83 0.2573172153164478 1104 84-85 0.2407124764235462 1104 86-87 0.2453871417959732 1104 88-89 0.23099786763421548 1104 90-91 0.1612915307106988 1104 92-93 0.19344304902114118 1104 94-95 0.17938795357562753 1104 96-97 0.182004495132837 1104 98-99 0.28679661672779844 1104 100 0.29669553780899705 1105 1 0.23623494349025975 1105 2 0.20402262794138437 1105 3 0.1683064594117738 1105 4 0.09513760655934078 1105 5 0.17038221670777176 1105 6 0.1070011345733235 1105 7 0.1040036172436416 1105 8 0.15519853031747743 1105 9 0.13554625377827278 1105 10-11 0.13344918049163113 1105 12-13 0.13259691302872767 1105 14-15 0.1515255868942944 1105 16-17 0.14362079426439323 1105 18-19 0.14425441753699886 1105 20-21 0.13704875709959907 1105 22-23 0.20471559991000277 1105 24-25 0.18487004574432575 1105 26-27 0.18020947034084855 1105 28-29 0.22481753402503557 1105 30-31 0.224857824523788 1105 32-33 0.22122615476681062 1105 34-35 0.13838141754761324 1105 36-37 0.16949968978717322 1105 38-39 0.13454499269439424 1105 40-41 0.19033157273130996 1105 42-43 0.14604924347959525 1105 44-45 0.10979577306487442 1105 46-47 0.17312668331157255 1105 48-49 0.2680368428882858 1105 50-51 0.27958300593596874 1105 52-53 0.24695287009049594 1105 54-55 0.14293170930861976 1105 56-57 0.10141706735930711 1105 58-59 0.1911587518751574 1105 60-61 0.16397199205593438 1105 62-63 0.06868139103509208 1105 64-65 0.03960575839920466 1105 66-67 0.10985990306510018 1105 68-69 0.19007871157629808 1105 70-71 0.14995302515630016 1105 72-73 0.10742936026255023 1105 74-75 0.09241167931353544 1105 76-77 0.14949502616898513 1105 78-79 0.26424390998346325 1105 80-81 0.21527754887477357 1105 82-83 0.19981543171557803 1105 84-85 0.14809675690256796 1105 86-87 0.1725709899608674 1105 88-89 0.132926805334165 1105 90-91 0.15106149151233694 1105 92-93 0.13522710283587713 1105 94-95 0.163348279675553 1105 96-97 0.16520818704782414 1105 98-99 0.18535082098770772 1105 100 0.23280229781021333 1106 1 0.1777135462118551 1106 2 0.1444614327888516 1106 3 0.10724133259513735 1106 4 0.06457133879769117 1106 5 0.19104283875519457 1106 6 0.07960582290306206 1106 7 0.07623224796452632 1106 8 0.16700509519800733 1106 9 0.09646191248343428 1106 10-11 0.07253437027417675 1106 12-13 0.06875310998731265 1106 14-15 0.1016122898635885 1106 16-17 0.10755433876759923 1106 18-19 0.12163739404329021 1106 20-21 0.12145798025562016 1106 22-23 0.17869888933047662 1106 24-25 0.17483399659314358 1106 26-27 0.1207833112840504 1106 28-29 0.14103063541485739 1106 30-31 0.14345755117828674 1106 32-33 0.18434837281148475 1106 34-35 0.15094378173419898 1106 36-37 0.13778203024055813 1106 38-39 0.1600676923565416 1106 40-41 0.13944177984814843 1106 42-43 0.1285071641812081 1106 44-45 0.14743852421874237 1106 46-47 0.14144567095853233 1106 48-49 0.2332073720343928 1106 50-51 0.18583862365952086 1106 52-53 0.17188084610605614 1106 54-55 0.16395065581056656 1106 56-57 0.14891537687053358 1106 58-59 0.27458846147525406 1106 60-61 0.24891335195671616 1106 62-63 0.18535304350892545 1106 64-65 0.15719816600269354 1106 66-67 0.14646704311872583 1106 68-69 0.16666063288061395 1106 70-71 0.17769028282161514 1106 72-73 0.07981556954218405 1106 74-75 0.13832473627755348 1106 76-77 0.19520074893302564 1106 78-79 0.2821150744799752 1106 80-81 0.24964367717135616 1106 82-83 0.20640717291598776 1106 84-85 0.17677105329996579 1106 86-87 0.22228288164056664 1106 88-89 0.18166519966626993 1106 90-91 0.1729850430229689 1106 92-93 0.1977452355825733 1106 94-95 0.23747603648070026 1106 96-97 0.09306878033505228 1106 98-99 0.1557379295211696 1106 100 0.2648841629478049 1107 1 0.1388389649015025 1107 2 0.11598618697407659 1107 3 0.09191727047941356 1107 4 0.060433906506830226 1107 5 0.15391111640568766 1107 6 0.09545959582690244 1107 7 0.06428865931473382 1107 8 0.14943523892961252 1107 9 0.08111950742071627 1107 10-11 0.05716437513998329 1107 12-13 0.05418801520307426 1107 14-15 0.04027284710847567 1107 16-17 0.06334562600363114 1107 18-19 0.034760409343320475 1107 20-21 0.06201016168887463 1107 22-23 0.1279540243600934 1107 24-25 0.1304390348849651 1107 26-27 0.07544132813915638 1107 28-29 0.09976754760709383 1107 30-31 0.12140589079729835 1107 32-33 0.12513366923608515 1107 34-35 0.1546313415536673 1107 36-37 0.12474340365655223 1107 38-39 0.12419534895333584 1107 40-41 0.08007684336266152 1107 42-43 0.10584169378859087 1107 44-45 0.09645024740819963 1107 46-47 0.06552734401703475 1107 48-49 0.22491256242191326 1107 50-51 0.16570796204291582 1107 52-53 0.1254023084694893 1107 54-55 0.05974500383138803 1107 56-57 0.04156431349044709 1107 58-59 0.19742527655062503 1107 60-61 0.11933308582017332 1107 62-63 0.10499564345187906 1107 64-65 0.08386343252183082 1107 66-67 0.1158953891460186 1107 68-69 0.13218308286376867 1107 70-71 0.14467197787072195 1107 72-73 0.05495131215189275 1107 74-75 0.052466914266737774 1107 76-77 0.27341475460189457 1107 78-79 0.2498147643462829 1107 80-81 0.16260388057474273 1107 82-83 0.09712009566260349 1107 84-85 0.13068853322524632 1107 86-87 0.15294628822737621 1107 88-89 0.1228409023355681 1107 90-91 0.0642955953253761 1107 92-93 0.04641842446923761 1107 94-95 0.09044625733936584 1107 96-97 0.06866499513713364 1107 98-99 0.1391969203397636 1107 100 0.10311979784200886 1108 1 -0.2868553675948675 1108 2 -0.10068960007350825 1108 3 -0.05864622903084893 1108 4 -0.016793485099469763 1108 5 0.042086051735502394 1108 6 0.0109435385064387 1108 7 -0.008472760061856377 1108 8 0.09960608884527744 1108 9 -0.00871008467925094 1108 10-11 -0.02540991870409215 1108 12-13 -0.030286835721184957 1108 14-15 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-0.9823402462820567 2316 50-51 -1.014254478608855 2316 52-53 -1.133823813044934 2316 54-55 -1.0920658224111008 2316 56-57 -1.2112588642408895 2316 58-59 -0.9891666063348126 2316 60-61 -0.9540465566593355 2316 62-63 -0.919778140611605 2316 64-65 -1.0830898104415851 2316 66-67 -2.3056335072774274 2316 68-69 -2.7034318415992757 2316 70-71 -2.5310137844234646 2316 72-73 -1.4534781812225717 2316 74-75 -1.017965770188347 2316 76-77 -2.3072456888447945 2316 78-79 -1.5426693742879465 2316 80-81 -1.1414299335174576 2316 82-83 -1.0591411865023588 2316 84-85 -1.0178187591965155 2316 86-87 -0.9778218621034469 2316 88-89 -0.8734274297021258 2316 90-91 -0.8812501216050777 2316 92-93 -0.8695598579973378 2316 94-95 -0.8390423875496467 2316 96-97 -0.8241203567544488 2316 98-99 -0.8271080918719527 2316 100 -0.812148647110007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 9.0 7 298.0 8 1349.0 9 1828.0 10 4828.0 11 5963.0 12 4323.0 13 3828.0 14 4786.0 15 9488.0 16 20792.0 17 32211.0 18 43910.0 19 56730.0 20 76583.0 21 107482.0 22 157228.0 23 249737.0 24 398418.0 25 516378.0 26 532156.0 27 486029.0 28 478630.0 29 540360.0 30 645148.0 31 782074.0 32 980898.0 33 1291239.0 34 1857178.0 35 3059324.0 36 5923864.0 37 1.1651898E7 38 1.1339418E7 39 1752890.0 40 570.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.450628927278768 28.96170429169034 27.41122814249032 26.17643863854057 2 23.600387430986007 30.768862584693572 25.459009618500055 20.17174036582036 3 24.907212344086506 24.12110369545476 26.80256530749042 24.169118652968322 4 20.425020390376623 27.689531706402192 23.312752415613968 28.572695487607213 5 24.132410630983493 28.90504161703126 25.8160258841418 21.146521867843447 6 28.67857281088813 27.520732384432556 23.966914195725053 19.83378060895426 7 23.435074444105698 24.79497055233706 28.918096664302915 22.851858339254324 8 26.506548619532193 26.342767751383178 24.591845546888738 22.55883808219589 9 25.194906904332377 24.007911135483425 27.298775659264194 23.498406300920003 10-11 22.989768548378002 27.554427703200844 26.666823965728643 22.788979782692508 12-13 22.754796294421535 25.990720827600732 26.680489224878652 24.57399365309908 14-15 22.696825312490958 29.22635999906501 25.180367437292674 22.896447251151354 16-17 24.53768164891513 25.59771067336162 25.28648256621168 24.578125111511557 18-19 22.740186003245544 27.276497809834005 25.415096151950696 24.568220034969755 20-21 24.439545960519407 27.442429996202737 25.33137701342315 22.786647029854702 22-23 24.482443274413214 25.827993010807493 26.859201105959627 22.830362608819666 24-25 22.897088405985937 27.164416302118344 25.161487052640595 24.777008239255128 26-27 22.959645416511716 25.530404303299157 28.63521270302463 22.874737577164495 28-29 22.977283629101404 25.601520857053895 28.53146106873421 22.889734445110495 30-31 22.911776837789752 25.467210011988566 28.680289130024338 22.940724020197344 32-33 22.985010930182934 25.694997925172803 28.41290585656619 22.907085288078076 34-35 23.033776017612485 25.77340288827543 28.26533759078682 22.92748350332526 36-37 22.93809215394087 25.72207377741972 28.431851225152982 22.907982843486423 38-39 22.94440916416643 25.73451758720502 28.555058885874963 22.766014362753594 40-41 22.978517424653216 25.851515639328902 28.412704869960116 22.757262066057766 42-43 22.976428390594243 25.82629368355574 28.39125129331433 22.80602663253569 44-45 23.07033822593862 25.91088520690444 28.358155326092305 22.66062124106464 46-47 23.111397229018955 26.02205878540235 28.215818187064762 22.650725798513925 48-49 23.170344886502754 26.08736414979275 28.072081836857976 22.67020912684652 50-51 23.185629762749745 26.173119624099655 27.980098564348193 22.66115204880241 52-53 23.317242783880545 26.257151292320696 27.68552989167013 22.740076032128627 54-55 23.383937822074746 26.445785662940953 27.23351209648102 22.936764418503277 56-57 23.66401662168558 26.801210662641207 26.63607504640048 22.89869766927273 58-59 23.79499323480057 27.100363790353715 26.112237904428167 22.992405070417547 60-61 23.752342859883786 27.215260336619995 25.971080281692398 23.061316521803818 62-63 23.751125311561996 27.402178806271376 25.865412382422047 22.981283499744578 64-65 23.72613981756555 27.52591549907097 25.727901745159183 23.0200429382043 66-67 23.6646850234307 27.491828339282144 25.775195566082953 23.068291071204204 68-69 23.55292424060193 27.54044892614263 25.84036454922557 23.06626228402987 70-71 23.51351924158449 27.425895810599172 25.922693769250994 23.137891178565344 72-73 23.431431742756732 27.358161149885298 25.968153430024294 23.24225367733368 74-75 23.4938111798067 27.376908770302645 25.90538811706926 23.2238919328214 76-77 23.543333364352485 27.388467157699807 25.784524313584996 23.28367516436272 78-79 23.501583505859085 27.40989536244643 25.677581682580346 23.41093944911414 80-81 23.52627714975823 27.48847924122621 25.56244386218836 23.422799746827195 82-83 23.519733198116825 27.527179291021888 25.42262916673726 23.530458344124032 84-85 23.435421067387008 27.501138392893242 25.449856723456804 23.613583816262953 86-87 23.441661816619153 27.47313447533531 25.427394439681528 23.657809268364012 88-89 23.414690594897085 27.40912037491502 25.413809325517377 23.76237970467052 90-91 23.381686915340456 27.26101128243783 25.510778569329933 23.846523232891776 92-93 23.423896342800823 27.192637378649607 25.572313018724735 23.81115325982483 94-95 23.394447284411076 27.087228072677615 25.619793625091575 23.89853101781973 96-97 23.325332863208533 27.00337896768471 25.72584167057674 23.945446498530014 98-99 23.349435504394343 26.926994838239455 25.802414303602113 23.92115535376409 100 23.276292383992452 26.892433445792392 25.801913513263635 24.02936065695152 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13401.0 1 11090.5 2 7325.5 3 5520.0 4 5202.0 5 5398.0 6 5792.5 7 6243.5 8 6478.0 9 6880.5 10 7706.0 11 11271.0 12 83765.5 13 104550.5 14 54630.5 15 57312.5 16 65376.5 17 73829.5 18 82766.5 19 91866.0 20 102119.0 21 117097.0 22 137406.5 23 160150.5 24 185084.0 25 214715.5 26 248379.0 27 288288.5 28 337674.5 29 389395.0 30 436423.0 31 485129.5 32 538172.0 33 585184.0 34 627858.5 35 680871.5 36 745153.5 37 806210.0 38 877785.5 39 954711.5 40 1015169.0 41 1086133.0 42 1174313.0 43 1238702.0 44 1290360.5 45 1362147.5 46 1440426.5 47 1510572.0 48 1584267.5 49 1655564.5 50 1709484.5 51 1754069.5 52 1774072.5 53 1756991.0 54 1713353.5 55 1649904.5 56 1565415.0 57 1447520.0 58 1296148.5 59 1119569.0 60 929632.0 61 758456.5 62 609132.5 63 469746.0 64 357433.0 65 271863.0 66 201350.0 67 149838.5 68 113498.0 69 88261.0 70 71170.0 71 59741.5 72 50036.5 73 37977.5 74 26944.5 75 19858.5 76 14753.0 77 10020.0 78 6538.0 79 4191.0 80 2423.5 81 1393.0 82 789.0 83 417.0 84 236.0 85 158.0 86 104.0 87 61.0 88 36.0 89 26.0 90 21.0 91 14.0 92 8.0 93 8.0 94 6.0 95 3.5 96 1.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02105405326556921 2 2.7662938485179813E-4 3 0.0 4 6.973850038280626E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0029766716580061137 16-17 0.0023339151461445823 18-19 2.3478628462211438E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.01375126996714968 28-29 0.03789590110801692 30-31 0.02372271321355126 32-33 0.041560659303133386 34-35 0.05850014104611703 36-37 0.08783331661546505 38-39 0.09981439098123115 40-41 0.057036794846417806 42-43 0.05274555245619579 44-45 0.045059207405670834 46-47 0.02670519641325594 48-49 0.040031061528070504 50-51 0.04565663389228354 52-53 0.052842024048392014 54-55 0.05295244334066479 56-57 0.070080219034682 58-59 0.07621372014334982 60-61 0.06799968710659494 62-63 0.07303131990921441 64-65 0.06112347096885025 66-67 0.09308927492764921 68-69 0.07474804932697117 70-71 0.07834655594672398 72-73 0.08334564411583147 74-75 0.07961463434535133 76-77 0.07821172817931722 78-79 0.06313891362991335 80-81 0.1005280483018152 82-83 0.11328554463850991 84-85 0.0969121070569667 86-87 0.09838010249002477 88-89 0.0905019765634471 90-91 0.10305258201567279 92-93 0.09879388425896277 94-95 0.1203430808772499 96-97 0.13065043123382866 98-99 0.15168588756596246 100 0.13049468191630706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.3017845E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.702158587991235 #Duplication Level Percentage of deduplicated Percentage of total 1 64.90388123856106 24.47016423432378 2 16.10005826374216 12.140138998710114 3 6.7690342497023614 7.656216083094682 4 3.4366741731946586 5.182801387721547 5 2.0721664121868715 3.9062573346489127 6 1.3867341147059207 3.1369721707212155 7 0.9496501599131159 2.5062702652508877 8 0.6948744861940102 2.0958614461788394 9 0.5370866351156924 1.8224392943360181 >10 2.805599878390309 18.922154522283105 >50 0.18795881126057778 4.981690122584258 >100 0.1546664089116486 9.389991255955522 >500 0.0010593642776456512 0.2679298072643347 >1k 4.8818915624994374E-4 0.31925707219224586 >5k 3.688074879805483E-5 0.09554245043076315 >10k+ 3.0733939006839405E-5 3.1063135543037044 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1245711 2.895800568345532 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 50726 0.11791850568060767 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.08101289127802659 1.6272316755988124E-5 0.0 2.324616679426875E-6 2.324616679426875E-6 2 0.08102451436142373 2.5570783473695626E-5 0.0 4.64923335885375E-6 4.64923335885375E-6 3 0.08103148821146201 3.951848355025687E-5 0.0 4.64923335885375E-6 4.64923335885375E-6 4 0.08104078667817972 4.184310022968375E-5 0.0 6.973850038280625E-6 1.1623083397134375E-5 5 0.08105008514489742 5.5790800306245E-5 0.0 6.973850038280625E-6 1.1623083397134375E-5 6 0.08106403284497399 5.811541698567188E-5 0.0 6.973850038280625E-6 1.6272316755988124E-5 7 0.08106868207833284 6.741388370337938E-5 0.0 6.973850038280625E-6 2.0921550114841875E-5 8 0.08108030516172998 6.973850038280625E-5 2.324616679426875E-6 6.973850038280625E-6 2.324616679426875E-5 9 0.08109657747848596 7.206311706223312E-5 2.324616679426875E-6 6.973850038280625E-6 2.324616679426875E-5 10-11 0.08112098595361994 8.136158377994063E-5 2.324616679426875E-6 9.2984667177075E-6 2.6733091813409065E-5 12-13 0.08119072445400274 8.71731254785078E-5 2.324616679426875E-6 1.6272316755988124E-5 3.2544633511976255E-5 14-15 0.08135344762156263 9.182235883736156E-5 2.324616679426875E-6 2.0921550114841875E-5 4.3005408569397186E-5 16-17 0.08154871542263449 1.0460775057420938E-4 2.324616679426875E-6 2.324616679426875E-5 5.811541698567187E-5 18-19 0.08173584706532835 1.1739314231105719E-4 2.324616679426875E-6 2.324616679426875E-5 6.857619204309282E-5 20-21 0.08200434029180216 1.3366545906704532E-4 2.324616679426875E-6 2.4408475133982188E-5 9.182235883736156E-5 22-23 0.08249483441116123 1.4063930910532594E-4 2.324616679426875E-6 2.9057708492835935E-5 1.0693236725363626E-4 24-25 0.08387449441040108 1.4528854246417968E-4 2.324616679426875E-6 3.719386687083E-5 1.1623083397134374E-4 26-27 0.08818084680903937 1.5574931752160062E-4 2.324616679426875E-6 3.951848355025687E-5 1.2669160902876469E-4 28-29 0.10051526291007837 1.5807393420102752E-4 2.324616679426875E-6 4.3005408569397186E-5 1.3715238408618563E-4 30-31 0.12832348993772236 1.6969701759816187E-4 2.324616679426875E-6 5.230387528710469E-5 1.429639257847528E-4 32-33 0.18319839127227316 1.708593259378753E-4 2.324616679426875E-6 5.811541698567187E-5 1.464508508038931E-4 34-35 0.27621792769953957 1.7550855929672908E-4 2.324616679426875E-6 6.044003366509875E-5 1.5807393420102752E-4 36-37 0.41616682565107577 1.7783317597615595E-4 2.324616679426875E-6 6.276465034452563E-5 1.7318394261730218E-4 38-39 0.605416194139897 1.8364471767472312E-4 2.324616679426875E-6 6.625157536366594E-5 1.7899548431586938E-4 40-41 0.8489058436097857 1.8480702601443656E-4 2.324616679426875E-6 6.741388370337938E-5 1.8132010099529625E-4 42-43 1.1479828894264694 1.8596933435415E-4 2.324616679426875E-6 7.090080872251969E-5 1.8364471767472312E-4 44-45 1.503934006922011 1.952678010718575E-4 2.324616679426875E-6 7.555004208137344E-5 1.9643010941157094E-4 46-47 1.9192476982517372 1.9759241775128437E-4 3.4869250191403125E-6 8.01992754402272E-5 2.1851396786612626E-4 48-49 2.391772530678838 2.103778094881322E-4 4.64923335885375E-6 8.36862004593675E-5 2.324616679426875E-4 50-51 2.915285505352488 2.1735165952641283E-4 4.64923335885375E-6 8.601081713879437E-5 2.510586013781025E-4 52-53 3.480881015773803 2.1851396786612626E-4 4.64923335885375E-6 8.949774215793469E-5 2.684932264738041E-4 54-55 4.090244409035367 2.2316320122498E-4 4.64923335885375E-6 9.066005049764813E-5 2.8011630987093844E-4 56-57 4.742169209080557 2.2781243458383374E-4 4.64923335885375E-6 9.066005049764813E-5 2.882524682489325E-4 58-59 5.439478662866538 2.324616679426875E-4 4.64923335885375E-6 9.066005049764813E-5 2.9173939326807284E-4 60-61 6.1775072647177005 2.3478628462211438E-4 4.64923335885375E-6 9.066005049764813E-5 2.998755516460669E-4 62-63 6.946486975347092 2.4176013466039502E-4 4.64923335885375E-6 9.066005049764813E-5 3.068494016843475E-4 64-65 7.7387244758541485 2.475716763589622E-4 4.64923335885375E-6 9.066005049764813E-5 3.103363267034878E-4 66-67 8.554911339701 2.568701430766697E-4 4.64923335885375E-6 9.066005049764813E-5 3.1498556006234155E-4 68-69 9.39654113310418 2.6035706809581E-4 4.64923335885375E-6 9.647159219621532E-5 3.277709517991894E-4 70-71 10.258191687658924 2.650063014546638E-4 4.64923335885375E-6 9.879620887564219E-5 3.3474480183747E-4 72-73 11.13549039939123 2.696555348135175E-4 4.64923335885375E-6 9.995851721535562E-5 3.498548102537447E-4 74-75 12.028460514467891 2.78954001531225E-4 4.64923335885375E-6 9.995851721535562E-5 3.6728943534944624E-4 76-77 12.939241842542323 2.847655432297922E-4 4.64923335885375E-6 9.995851721535562E-5 3.742632853877269E-4 78-79 13.862298308992466 2.8709015990921905E-4 4.64923335885375E-6 9.995851721535562E-5 3.777502104068672E-4 80-81 14.800636805493163 2.940640099474997E-4 4.64923335885375E-6 1.022831338947825E-4 3.8239944376572096E-4 82-83 15.747786761517226 3.0452478500492063E-4 4.64923335885375E-6 1.022831338947825E-4 3.975094521819956E-4 84-85 16.704922108487768 3.149855600623416E-4 4.64923335885375E-6 1.022831338947825E-4 4.102948439188434E-4 86-87 17.668438528243335 3.289332601389028E-4 4.64923335885375E-6 1.022831338947825E-4 4.323787023733988E-4 88 18.393652680649158 3.3474480183747E-4 4.64923335885375E-6 1.0693236725363625E-4 4.370279357322525E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 173710 0.0 78.31799 8 GTGGTAT 175205 0.0 77.84502 6 AGTGGTA 175545 0.0 77.496284 5 TGGTATC 176760 0.0 77.01939 7 GTATCAA 176755 0.0 76.90656 9 AAGCAGT 185060 0.0 74.228546 1 CAGTGGT 193005 0.0 70.78982 4 GCAGTGG 194065 0.0 70.59432 3 AGCAGTG 204820 0.0 67.139984 2 CAACGCA 169510 0.0 42.759163 12-13 ATCAACG 172375 0.0 42.187378 10-11 ACGCAGA 175380 0.0 41.31591 14-15 AGAGTAC 175135 0.0 40.852943 18-19 TCAACGC 168750 0.0 40.13366 12-13 AGTACTT 174335 0.0 39.695766 20-21 GAGTACT 163815 0.0 39.574207 20-21 AACGCAG 171870 0.0 39.312138 14-15 TATCAAC 179775 0.0 37.799137 10-11 GTACTTT 173125 0.0 37.364586 22-23 CGCAGAG 180635 0.0 37.312714 16-17 >>END_MODULE