FastQCFastQC Report
Thu 26 May 2016
SRR1660651_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660651_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27584876
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9549503.461860767472727No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT315620.11441777008531777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1326950.079.747388
AGTGGTA1345900.078.903625
GTGGTAT1347850.078.716326
GTATCAA1341800.078.567389
TGGTATC1357100.077.9306957
AAGCAGT1416950.075.919791
GCAGTGG1475800.072.849293
CAGTGGT1479850.072.088364
AGCAGTG1540600.070.001522
CAACGCA1299950.043.04733712-13
ATCAACG1320300.042.47661610-11
ACGCAGA1340400.041.844614-15
AGAGTAC1340650.041.29747418-19
TCAACGC1303200.040.500612-13
AGTACTT1335750.040.15993520-21
GAGTGTA295300.039.92333694
AACGCAG1320900.039.8020914-15
GAGTACT1267600.039.73063320-21
CGCAGAG1358050.038.6213716-17
TATCAAC1372500.038.3639510-11
Produced by FastQC (version 0.11.5)