FastQCFastQC Report
Thu 26 May 2016
SRR1660651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27584876
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7800422.8277886766647056No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTGTTTTTTTTTTTTTTTT513950.18631586380884946No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT277240.10050434883230941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1277350.078.903828
AGTGGTA1299950.078.492585
GTATCAA1288600.077.876169
GTGGTAT1309550.077.7881246
TGGTATC1311700.077.1810767
AAGCAGT1342950.076.059391
GCAGTGG1419100.072.494313
CAGTGGT1431450.071.6688164
AGCAGTG1478800.069.418482
CAACGCA1242350.042.753412-13
ATCAACG1259600.042.1730610-11
ACGCAGA1288300.041.36353714-15
AGAGTAC1304250.040.81199318-19
TCAACGC1240950.040.2746612-13
AGTACTT1293800.039.915620-21
GAGTACT1226900.039.59921620-21
AACGCAG1268200.039.45746614-15
TATCAAC1317950.038.08342410-11
CGCAGAG1313600.038.0403616-17
GTACTTT1290200.037.69901322-23
Produced by FastQC (version 0.11.5)