FastQCFastQC Report
Thu 26 May 2016
SRR1660650_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660650_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35660870
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12457033.493192959117374No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT458050.12844610913867216No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC363230.10185674101613337Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1720600.079.98268
AGTGGTA1738850.079.160545
GTGGTAT1739850.079.057146
GTATCAA1740500.078.806499
TGGTATC1745800.078.617587
AAGCAGT1804250.077.30461
GCAGTGG1871100.074.449523
CAGTGGT1892450.072.990354
AGCAGTG1945300.071.878272
CAACGCA1687250.043.19870812-13
ATCAACG1708150.042.7433210-11
ACGCAGA1733050.042.0216314-15
AGAGTAC1727100.041.38884718-19
TCAACGC1683200.040.84328512-13
AGTACTT1722200.040.1690420-21
GAGTACT1627250.040.07109520-21
AACGCAG1712900.040.00129714-15
CGCAGAG1752900.038.9952916-17
TATCAAC1772750.038.68798410-11
GTACTTT1704550.038.2268122-23
Produced by FastQC (version 0.11.5)