##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660650_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35660870 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99699945626677 34.0 31.0 34.0 30.0 34.0 2 32.20201128015105 34.0 31.0 34.0 30.0 34.0 3 32.25651600760161 34.0 31.0 34.0 30.0 34.0 4 35.70846263705849 37.0 35.0 37.0 35.0 37.0 5 35.73309139681673 37.0 35.0 37.0 35.0 37.0 6 35.75119129174358 37.0 35.0 37.0 35.0 37.0 7 35.681065240416174 37.0 35.0 37.0 33.0 37.0 8 35.71297680062208 37.0 35.0 37.0 35.0 37.0 9 37.4068457948446 39.0 37.0 39.0 35.0 39.0 10-11 37.31054926590406 39.0 37.0 39.0 34.0 39.0 12-13 37.367697016365554 39.0 37.0 39.0 34.0 39.0 14-15 38.76800724435495 40.0 38.0 41.0 35.0 41.0 16-17 38.72041223335269 40.0 38.0 41.0 34.5 41.0 18-19 38.66731491688228 40.0 38.0 41.0 34.0 41.0 20-21 38.5501505291374 40.0 38.0 41.0 34.0 41.0 22-23 38.39614158319749 40.0 38.0 41.0 34.0 41.0 24-25 38.37380149446719 40.0 38.0 41.0 34.0 41.0 26-27 38.31595785520656 40.0 38.0 41.0 34.0 41.0 28-29 38.24029608643872 40.0 38.0 41.0 33.5 41.0 30-31 38.094391022989626 40.0 38.0 41.0 33.0 41.0 32-33 38.19246517261077 40.0 38.0 41.0 33.0 41.0 34-35 38.12507887496856 40.0 38.0 41.0 33.0 41.0 36-37 37.97366703336178 40.0 38.0 41.0 33.0 41.0 38-39 37.64116074565763 40.0 37.0 41.0 33.0 41.0 40-41 37.48032311045692 40.0 36.5 41.0 32.0 41.0 42-43 37.27769486554871 40.0 36.0 41.0 31.5 41.0 44-45 37.43385570795104 40.0 36.5 41.0 32.0 41.0 46-47 37.375624767427155 40.0 36.0 41.0 32.0 41.0 48-49 37.22944008376689 40.0 36.0 41.0 31.0 41.0 50-51 36.058230197412456 38.5 34.5 40.0 29.5 40.5 52-53 36.173909245624124 38.5 35.0 40.0 30.0 40.5 54-55 36.48491551103493 39.0 35.0 41.0 29.5 41.0 56-57 36.18696710147565 39.0 35.0 41.0 28.0 41.0 58-59 35.946891592942066 39.0 35.0 41.0 28.0 41.0 60-61 35.683365240388135 39.0 35.0 41.0 27.0 41.0 62-63 35.39330572978169 38.0 35.0 40.5 26.5 41.0 64-65 35.07132819530202 37.5 34.0 40.0 26.0 41.0 66-67 34.698344810432275 37.0 34.0 40.0 26.0 41.0 68-69 34.35125009569312 36.0 34.0 39.0 26.0 41.0 70-71 34.01864181945085 36.0 33.5 39.0 25.5 41.0 72-73 33.620045262496404 35.0 33.0 38.5 25.0 40.5 74-75 33.23641245993157 35.0 33.0 37.5 24.0 40.0 76-77 32.875146428003575 35.0 33.0 37.0 24.0 39.0 78-79 32.505237126856414 35.0 32.0 36.5 23.0 39.0 80-81 32.141004383796584 35.0 32.0 36.0 22.5 38.5 82-83 31.805116728784242 35.0 32.0 35.5 20.5 37.0 84-85 31.515763314243316 35.0 31.0 35.0 20.0 37.0 86-87 31.234277402654506 35.0 31.0 35.0 20.0 36.0 88-89 30.999966111314727 34.0 31.0 35.0 19.0 36.0 90-91 30.78069249292011 34.0 30.5 35.0 19.0 36.0 92-93 30.572318300142424 34.0 30.0 35.0 18.0 35.0 94-95 30.371027389404688 34.0 30.0 35.0 18.0 35.0 96-97 30.195274456287805 34.0 30.0 35.0 17.0 35.0 98-99 30.015010416178853 34.0 29.0 35.0 17.0 35.0 100 29.839514010735016 34.0 29.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38161813543939616 1101 2 0.32984971863774604 1101 3 0.3232544597603493 1101 4 0.248794127000302 1101 5 0.25373255752017343 1101 6 0.22138511800499572 1101 7 0.2763234827129608 1101 8 0.28513313967196297 1101 9 0.33379743443422427 1101 10-11 0.3115507885952482 1101 12-13 0.31191414750124125 1101 14-15 0.373496474859877 1101 16-17 0.4161312514582818 1101 18-19 0.36909583146000813 1101 20-21 0.40502919662446146 1101 22-23 0.39801561771439964 1101 24-25 0.3973613500440152 1101 26-27 0.3399843654939545 1101 28-29 0.3664422039376021 1101 30-31 0.3497958545265831 1101 32-33 0.3181790243962155 1101 34-35 0.298814161321161 1101 36-37 0.3067429494404692 1101 38-39 0.2682026713728547 1101 40-41 0.285791084706176 1101 42-43 0.19102703817667788 1101 44-45 0.2580852082175724 1101 46-47 0.36003346786093715 1101 48-49 0.3562614687854264 1101 50-51 0.3177842813540863 1101 52-53 0.3888933585933998 1101 54-55 0.411018738968572 1101 56-57 0.4677716911173917 1101 58-59 0.3950027419401252 1101 60-61 0.3975171784607383 1101 62-63 0.3724412949779392 1101 64-65 0.38294564087020433 1101 66-67 0.4184408873059695 1101 68-69 0.3207623889560409 1101 70-71 0.36004296342170505 1101 72-73 0.3002792942297461 1101 74-75 0.25137811925952036 1101 76-77 0.2808735646349021 1101 78-79 0.2463755075660643 1101 80-81 0.28674650404641966 1101 82-83 0.2163184221100849 1101 84-85 0.2214481894840894 1101 86-87 0.25526932523165513 1101 88-89 0.21180136229289204 1101 90-91 0.18493240430968072 1101 92-93 0.141599496015953 1101 94-95 0.08770403850852304 1101 96-97 0.1359720542275369 1101 98-99 0.01746690639950188 1101 100 -0.06145929036554065 1102 1 0.2573116807923128 1102 2 0.24256567198185763 1102 3 0.23407368954305952 1102 4 0.22030264030276214 1102 5 0.20456600302674133 1102 6 0.22148693986276413 1102 7 0.21527317806685176 1102 8 0.2190796013827736 1102 9 0.2792801004543932 1102 10-11 0.24344437765386573 1102 12-13 0.2608740075970104 1102 14-15 0.28579632760791895 1102 16-17 0.30736667645394533 1102 18-19 0.28736723764562555 1102 20-21 0.36214339483495195 1102 22-23 0.31479125248731776 1102 24-25 0.2577280642275497 1102 26-27 0.3268976651779596 1102 28-29 0.3053643926700502 1102 30-31 0.31138131259491786 1102 32-33 0.2669664536005527 1102 34-35 0.22418195095323767 1102 36-37 0.21042683441495313 1102 38-39 0.20478950258551976 1102 40-41 0.24720682593304133 1102 42-43 0.22901792715594382 1102 44-45 0.3056187355939741 1102 46-47 0.2843109703925606 1102 48-49 0.3124715097416484 1102 50-51 0.26135996240481774 1102 52-53 0.2984472562992835 1102 54-55 0.3546335563198326 1102 56-57 0.38114392723300483 1102 58-59 0.344236586129135 1102 60-61 0.2854900779871201 1102 62-63 0.30361529592477865 1102 64-65 0.31225297773347904 1102 66-67 0.32051294524244867 1102 68-69 0.32786166139121775 1102 70-71 0.300004014755487 1102 72-73 0.23049482443448 1102 74-75 0.18861933265898045 1102 76-77 0.1893944689349496 1102 78-79 0.17094487043343776 1102 80-81 0.15199376856771352 1102 82-83 0.2029110228031783 1102 84-85 0.17001829226246556 1102 86-87 0.20541771803749498 1102 88-89 0.15298060470498243 1102 90-91 0.17452316551167968 1102 92-93 0.13949958982761146 1102 94-95 0.1265481163333 1102 96-97 0.043068750814377665 1102 98-99 0.007558324848929487 1102 100 0.06436758706514922 1103 1 0.25014205913181087 1103 2 0.2220642508562065 1103 3 0.24927170704716417 1103 4 0.22225827228333372 1103 5 0.195930811302091 1103 6 0.1880693133301179 1103 7 0.14466586931382608 1103 8 0.15574859435628952 1103 9 0.18638939082339334 1103 10-11 0.19587915225488217 1103 12-13 0.2163395138943116 1103 14-15 0.19982241499090492 1103 16-17 0.27429806042703575 1103 18-19 0.28150721120960753 1103 20-21 0.24570166767145452 1103 22-23 0.3348934203348648 1103 24-25 0.2605507926817765 1103 26-27 0.2962495804192713 1103 28-29 0.2734912977598114 1103 30-31 0.2656704767430469 1103 32-33 0.25926799036858483 1103 34-35 0.2553860265272192 1103 36-37 0.27515245101036356 1103 38-39 0.2889370781701288 1103 40-41 0.2472234374039104 1103 42-43 0.27982912818227845 1103 44-45 0.3133864672130997 1103 46-47 0.3329236542567173 1103 48-49 0.2839255227715327 1103 50-51 0.24109102751852873 1103 52-53 0.3430451567129893 1103 54-55 0.4083358143211271 1103 56-57 0.4160223971962864 1103 58-59 0.41646866716408937 1103 60-61 0.37297537828469984 1103 62-63 0.3639191198606184 1103 64-65 0.3324760828048454 1103 66-67 0.3395973463873716 1103 68-69 0.3426601264631941 1103 70-71 0.3145945137618398 1103 72-73 0.25896907368980493 1103 74-75 0.2449195539532596 1103 76-77 0.24682331876446995 1103 78-79 0.19423301987668395 1103 80-81 0.26893422672449674 1103 82-83 0.26216190417075325 1103 84-85 0.2695077209273329 1103 86-87 0.24078417733167967 1103 88-89 0.18427486911663138 1103 90-91 0.15949987254323972 1103 92-93 0.15729436529539598 1103 94-95 0.1732933727630268 1103 96-97 0.0346226645109553 1103 98-99 0.04431141936955996 1103 100 -0.0026632627731473235 1104 1 0.2056317033769588 1104 2 0.158105309186638 1104 3 0.17909106212643877 1104 4 0.15476740310112547 1104 5 0.13797431597661358 1104 6 0.1275783338869374 1104 7 0.11535946040139322 1104 8 0.08736158942238603 1104 9 0.16385195785017714 1104 10-11 0.1385796431971258 1104 12-13 0.1657068919116682 1104 14-15 0.17962536777255877 1104 16-17 0.22027730675294777 1104 18-19 0.21786984375097518 1104 20-21 0.19236397041326825 1104 22-23 0.25944647887173744 1104 24-25 0.25236690292950925 1104 26-27 0.24135879777822566 1104 28-29 0.25950165152443816 1104 30-31 0.25760380298454066 1104 32-33 0.21473426048567745 1104 34-35 0.18593705905347235 1104 36-37 0.1814817389335417 1104 38-39 0.1915090762978977 1104 40-41 0.25202425292693675 1104 42-43 0.23330773807180805 1104 44-45 0.24708974206226486 1104 46-47 0.2261270083367677 1104 48-49 0.20300009063425506 1104 50-51 0.17371013914739564 1104 52-53 0.2395008970095276 1104 54-55 0.28958226584089886 1104 56-57 0.29903428393605225 1104 58-59 0.28544565019728196 1104 60-61 0.3005576131534369 1104 62-63 0.2627782608027118 1104 64-65 0.22891764097956013 1104 66-67 0.2752978667746362 1104 68-69 0.2368200865607193 1104 70-71 0.24761669656149365 1104 72-73 0.21285345723550364 1104 74-75 0.15913914290397457 1104 76-77 0.1382522546622056 1104 78-79 0.09198488693041895 1104 80-81 0.09607331465451097 1104 82-83 0.12491884337363857 1104 84-85 0.1232980264092376 1104 86-87 0.12333679887623461 1104 88-89 0.1340785169585672 1104 90-91 0.15212299147952635 1104 92-93 0.13617574415792788 1104 94-95 0.1024140890335481 1104 96-97 0.10641758326472939 1104 98-99 0.007869308974061795 1104 100 -0.060903679619322304 1105 1 0.16262864866751414 1105 2 0.08723704600333804 1105 3 0.15344084410021708 1105 4 0.12107134493723493 1105 5 0.16122518660176155 1105 6 0.1605569932791937 1105 7 0.16009689270344296 1105 8 0.14800400271438008 1105 9 0.18854135691860563 1105 10-11 0.17286781342490798 1105 12-13 0.1614589326508451 1105 14-15 0.18725490398942668 1105 16-17 0.24753847410107 1105 18-19 0.24145700031005646 1105 20-21 0.2214443231993144 1105 22-23 0.22166494494727118 1105 24-25 0.24437145098077195 1105 26-27 0.22735384774353662 1105 28-29 0.19175148801704722 1105 30-31 0.21213034546273235 1105 32-33 0.20210036711058876 1105 34-35 0.20524539227108818 1105 36-37 0.1514224947661873 1105 38-39 0.20230557155215223 1105 40-41 0.23954242177877205 1105 42-43 0.259964534684606 1105 44-45 0.2186998652563048 1105 46-47 0.23211877062177422 1105 48-49 0.22539936229524926 1105 50-51 0.17803094212684556 1105 52-53 0.20885395894934078 1105 54-55 0.27376941423688805 1105 56-57 0.3042353829525979 1105 58-59 0.288606900677145 1105 60-61 0.31866392332559457 1105 62-63 0.32837423631173834 1105 64-65 0.31097702624786905 1105 66-67 0.31078842472231827 1105 68-69 0.2933788194124318 1105 70-71 0.30521798453549565 1105 72-73 0.20335631102336293 1105 74-75 0.20399857984384795 1105 76-77 0.20725043928480602 1105 78-79 0.2349530801663562 1105 80-81 0.21529669275444263 1105 82-83 0.28757648967786054 1105 84-85 0.24894582496083828 1105 86-87 0.19435633182085255 1105 88-89 0.20430645390972657 1105 90-91 0.2190073477456913 1105 92-93 0.2061190807635107 1105 94-95 0.14465977104277172 1105 96-97 0.15194624688580305 1105 98-99 0.13727275629760882 1105 100 0.09748740201487394 1106 1 0.0536742586175194 1106 2 0.050978820474156805 1106 3 0.07602002550647313 1106 4 0.09640682867684092 1106 5 0.06784329225691721 1106 6 0.12519959637610611 1106 7 0.1109190867202301 1106 8 0.11409829424803064 1106 9 0.17293098186046052 1106 10-11 0.11481270849009917 1106 12-13 0.11755790257077336 1106 14-15 0.1594044123037932 1106 16-17 0.11817139614738892 1106 18-19 0.1729256973926212 1106 20-21 0.1503474517974226 1106 22-23 0.1811276473899852 1106 24-25 0.15910512591324277 1106 26-27 0.18522407854111123 1106 28-29 0.1492064862291116 1106 30-31 0.1793234572567428 1106 32-33 0.15198227210519377 1106 34-35 0.14530746674412853 1106 36-37 0.1351361135336333 1106 38-39 0.16983182836177946 1106 40-41 0.20554579160558006 1106 42-43 0.19867344269425757 1106 44-45 0.19667972098448416 1106 46-47 0.19255480447993278 1106 48-49 0.14919594688560522 1106 50-51 0.11110399448114094 1106 52-53 0.14599978301104954 1106 54-55 0.18941779062760133 1106 56-57 0.23736729045715776 1106 58-59 0.2569781898352872 1106 60-61 0.27351947087741735 1106 62-63 0.23942872543730687 1106 64-65 0.1944533290391064 1106 66-67 0.2790357216312316 1106 68-69 0.2841245941568573 1106 70-71 0.24932578408850503 1106 72-73 0.21267286784740946 1106 74-75 0.15752414643667834 1106 76-77 0.20769329086351718 1106 78-79 0.18080538484396413 1106 80-81 0.25259070793864424 1106 82-83 0.24062354442915534 1106 84-85 0.21311529739255874 1106 86-87 0.1311795245689602 1106 88-89 0.1119770416571022 1106 90-91 0.22834199508065822 1106 92-93 0.08668564497670772 1106 94-95 0.015824054932053855 1106 96-97 0.13455013760297874 1106 98-99 0.06529193167619951 1106 100 0.06846331658262628 1107 1 0.1049714454920121 1107 2 0.0803379676853524 1107 3 0.08517043231955057 1107 4 0.06139374739350956 1107 5 0.053733086132652375 1107 6 0.07252845825739485 1107 7 0.07786702189845585 1107 8 0.07545768373876172 1107 9 0.111919615005732 1107 10-11 0.07283519425534024 1107 12-13 0.09366368957387294 1107 14-15 0.07476200359196383 1107 16-17 0.13215825732002884 1107 18-19 0.10487081720585678 1107 20-21 0.1407305495517832 1107 22-23 0.1494768456763822 1107 24-25 0.07387753258842622 1107 26-27 0.08272299701769015 1107 28-29 0.12888675850825138 1107 30-31 0.05851518054281968 1107 32-33 0.09173933707746329 1107 34-35 0.13986486492642314 1107 36-37 0.12378127689983387 1107 38-39 0.11107061692958808 1107 40-41 0.10781236317360054 1107 42-43 0.14949217848867846 1107 44-45 0.09512842206477501 1107 46-47 0.1362617965405306 1107 48-49 0.14909935612575254 1107 50-51 0.12272929685337886 1107 52-53 0.16801273634643366 1107 54-55 0.228627813478262 1107 56-57 0.19815543889908582 1107 58-59 0.21569845634137863 1107 60-61 0.1566879651637194 1107 62-63 0.13375228568558128 1107 64-65 0.14386528921482977 1107 66-67 0.1697035567824159 1107 68-69 0.18970795190960388 1107 70-71 0.17184595788027934 1107 72-73 0.10937479798607086 1107 74-75 0.08935813379166291 1107 76-77 0.06674648151463458 1107 78-79 0.10811502251554117 1107 80-81 0.16049093858799068 1107 82-83 0.18675504979628954 1107 84-85 0.15373267288550707 1107 86-87 0.10552312372093908 1107 88-89 0.07252280764176433 1107 90-91 0.1502319377158905 1107 92-93 0.09937238929679637 1107 94-95 0.04934647491132793 1107 96-97 0.0432604170268327 1107 98-99 0.029038019196711673 1107 100 -0.01191451037781377 1108 1 0.051791785158464165 1108 2 0.036375253885999825 1108 3 0.04244584895571535 1108 4 0.019019598739880905 1108 5 0.057044026227409006 1108 6 0.07673686830774074 1108 7 0.04542062753700549 1108 8 0.06937244259771802 1108 9 0.07541650645095643 1108 10-11 0.08851359789627367 1108 12-13 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-0.9502211161236467 2316 92-93 -0.9507829877730778 2316 94-95 -0.8876601775800985 2316 96-97 -0.9381164617800337 2316 98-99 -0.9294850610729881 2316 100 -0.9687120251606878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1752.0 3 1943.0 4 1795.0 5 2867.0 6 3801.0 7 7191.0 8 15805.0 9 23914.0 10 28306.0 11 29443.0 12 32886.0 13 38544.0 14 46549.0 15 57454.0 16 74287.0 17 94764.0 18 107803.0 19 122195.0 20 142082.0 21 174041.0 22 226035.0 23 319757.0 24 451120.0 25 538932.0 26 537626.0 27 492926.0 28 470454.0 29 515417.0 30 615721.0 31 758089.0 32 962831.0 33 1261203.0 34 1748500.0 35 2620421.0 36 4407233.0 37 7759441.0 38 8635614.0 39 2327153.0 40 4975.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.081548807223026 28.768801088109903 27.979669413291642 25.169980691375432 2 23.023860111344945 32.88722046646383 24.261390190356874 19.827529231834355 3 25.529145727769304 23.780547359265615 27.738191802665362 22.952115110299715 4 19.348880462846214 27.735687766166134 22.99885194950373 29.916579821483925 5 23.151162836354782 30.115142142517783 26.442970506850923 20.290724514276516 6 29.82697570487848 29.252389495575738 23.049926709196946 17.870708090348835 7 22.849364345903194 24.84447274653306 30.28364741499826 22.02251549256549 8 26.8921265439385 27.42947101430864 24.336548908448233 21.34185353330463 9 25.014467072858043 24.242240173404642 28.01817493420444 22.725117819532876 10-11 22.283017682663317 28.588586769510087 27.304603247117278 21.823792300709318 12-13 21.95106304115354 26.672479384024534 27.39494595915859 23.981511615663344 14-15 21.917651066409526 30.534137568450088 25.7032425033181 21.84496886182228 16-17 24.082971328724476 26.124005955546803 25.785722190692134 24.007300525036587 18-19 21.843090346677865 28.24081589957191 25.993405866399517 23.922687887350712 20-21 23.878073564907332 28.533642344371728 25.89896029394308 21.689323796777863 22-23 23.98557431494279 26.386811302549244 27.768719720381018 21.858894662126946 24-25 21.97707036949008 28.11478849491735 25.762507571535288 24.145633564057288 26-27 22.009979751432343 26.23744567756927 29.941187944033686 21.811386626964694 28-29 22.08390296056497 26.20557793296785 29.8762940479669 21.83422505850028 30-31 21.91053295430253 26.049164205476405 30.08504061418154 21.955262226039522 32-33 22.071840581401784 26.292523531165045 29.77067511842384 21.86496076900933 34-35 22.105650919191618 26.47267902972756 29.55662855432949 21.86504149675134 36-37 21.914913129285814 26.406589309948046 29.814393412879248 21.86410414788689 38-39 21.964688718090866 26.358072959676182 29.869229417194425 21.808008905038527 40-41 22.07650932143771 26.379451315130808 29.72208443402341 21.821954929408065 42-43 22.064995597916433 26.387356470664365 29.776075462942774 21.77157246847643 44-45 22.170151942041613 26.514882981612477 29.67511491887701 21.639850157468903 46-47 22.125680578947915 26.546835579652335 29.58926114199747 21.738222699402282 48-49 22.202275155423663 26.57876730943552 29.490637314536826 21.72832022060399 50-51 22.336265562317916 26.668371631525083 29.372164230716603 21.6231985754404 52-53 22.450965991588838 26.759441481502357 29.1783171086365 21.611275418272314 54-55 22.681882982213477 26.921939585023587 28.632449709837065 21.76372772292587 56-57 23.064801078254447 27.387294180727746 27.86042260896734 21.68748213205047 58-59 23.305079911611294 27.64432236980226 27.365521790301646 21.6850759282848 60-61 23.43784138005067 27.820171601249154 27.158464892186174 21.583522126513998 62-63 23.575744996689664 27.99019785381703 26.985798231902937 21.448258917590376 64-65 23.782544393437156 27.96922171030945 26.878212269805896 21.3700216264475 66-67 23.87237276418021 27.858316168025492 26.99834178811135 21.270969279682944 68-69 24.02686148558273 27.792807907200164 27.088070396685776 21.09226021053133 70-71 24.203267075648917 27.651880450011824 27.14186085633683 21.002991618002426 72-73 24.338280748710165 27.596021735790426 27.176013622303575 20.889683893195834 74-75 24.59363808209173 27.661047604046345 27.03842481456386 20.706889499298068 76-77 24.861892481421034 27.674598211714716 26.79225434861635 20.671254958247896 78-79 25.072851416679566 27.717353526658904 26.60371320380869 20.60608185285283 80-81 25.329346777284595 27.835148860309904 26.349542779619956 20.485961582785542 82-83 25.529603929938325 27.84123403925672 26.13359309128428 20.49556893952067 84-85 25.659955614241824 27.817822505496714 26.053274108910724 20.46894777135074 86-87 25.851409593725762 27.846097796171996 25.934828609626415 20.36766400047583 88-89 26.025279038094695 27.761101735925152 25.815840471386597 20.39777875459356 90-91 26.163117335837338 27.70168580944312 25.78096736559012 20.35422948912942 92-93 26.287176142138996 27.741941053249487 25.728180789742357 20.242702014869156 94-95 26.373532933938392 27.706173990821885 25.65669129245706 20.263601782782658 96-97 26.434231755618192 27.694437589860115 25.64373016142959 20.2276004930921 98-99 26.554188983670045 27.772117242274696 25.553479300611325 20.120214473443934 100 26.565192309306546 27.791776092003495 25.450527039735675 20.192504558954283 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18813.0 1 14596.0 2 8844.5 3 7016.0 4 6821.5 5 6998.0 6 7289.5 7 7783.5 8 8012.0 9 8146.5 10 8638.0 11 11582.5 12 82427.5 13 100988.5 14 51080.0 15 54653.5 16 63078.5 17 71924.5 18 80447.0 19 89425.0 20 99618.5 21 113374.0 22 132341.0 23 156049.0 24 182461.0 25 211275.5 26 245206.0 27 285157.0 28 331555.0 29 381258.0 30 428998.0 31 476528.0 32 528207.5 33 580564.0 34 634004.5 35 694089.5 36 760591.5 37 819117.0 38 877519.0 39 937079.5 40 980979.5 41 1026251.5 42 1077238.0 43 1116212.0 44 1152533.0 45 1198775.0 46 1244410.0 47 1286812.0 48 1323723.5 49 1349313.0 50 1361290.0 51 1355376.5 52 1337008.5 53 1301199.0 54 1241137.0 55 1166037.5 56 1081529.5 57 985325.5 58 876591.0 59 758961.0 60 632900.0 61 512037.5 62 405310.5 63 311595.5 64 236313.5 65 178406.0 66 132903.0 67 99207.5 68 75607.5 69 58708.5 70 46252.5 71 37572.0 72 31373.0 73 25263.5 74 19677.0 75 15945.5 76 12710.5 77 9153.0 78 6510.0 79 4807.5 80 3446.0 81 2487.0 82 1791.5 83 1291.0 84 962.0 85 707.5 86 579.5 87 486.0 88 392.0 89 325.5 90 261.0 91 217.5 92 184.0 93 153.5 94 129.5 95 98.5 96 75.5 97 61.0 98 45.5 99 33.5 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07150414445861808 2 0.03670690030837722 3 0.027806388346666808 4 0.02071177736269474 5 0.01629236751655246 6 0.015832479689923438 7 0.016446598190117068 8 0.007156303253397912 9 0.006474884095648816 10-11 0.007795659500174842 12-13 0.011204157385952726 14-15 0.01391441094959265 16-17 0.027300231317968406 18-19 0.048284015504949825 20-21 0.07504023317434487 22-23 0.07357223758141627 24-25 0.07454809711597052 26-27 0.08053645354137462 28-29 0.07110735099844731 30-31 0.06487082339830745 32-33 0.07067550511246641 34-35 0.08381175220907397 36-37 0.08789045247634172 38-39 0.07854267156129394 40-41 0.07139618298712286 42-43 0.07142843121886819 44-45 0.06727822400294776 46-47 0.07045677797541114 48-49 0.0697879776909537 50-51 0.07762570010210071 52-53 0.077799560134119 54-55 0.08250920406596922 56-57 0.08814142784514231 58-59 0.08369537815538432 60-61 0.08088697779947601 62-63 0.08212222528502529 64-65 0.08660753369169064 66-67 0.08587143274967773 68-69 0.08058692903454122 70-71 0.08408095483929584 72-73 0.10852511450225415 74-75 0.10795866730116233 76-77 0.1080554119963983 78-79 0.09362783353294521 80-81 0.09476633632325851 82-83 0.09206169114774822 84-85 0.09253980623579851 86-87 0.09730693614597737 88-89 0.0978944148025553 90-91 0.10933833078104938 92-93 0.11167001814593978 94-95 0.12360186389171099 96-97 0.11768221022089478 98-99 0.11622963769532264 100 0.11303986694660001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.566087E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.05534187300069 #Duplication Level Percentage of deduplicated Percentage of total 1 76.14285267794361 38.874993746579364 2 12.617825444519543 12.884147835275842 3 4.326427296952506 6.626616742037775 4 2.0437846208168655 4.173844901223446 5 1.1497769533390239 2.935112771521051 6 0.7634695660513088 2.3387519822628633 7 0.5405029111470707 1.9316892638376069 8 0.37947339054840723 1.5499314948924514 9 0.2958959681848902 1.3596362833069895 >10 1.5587083860339352 14.074279527695301 >50 0.12557656515573412 4.514631263295989 >100 0.05471730722325897 4.295125354064825 >500 5.562325479924855E-4 0.1833587224462146 >1k 3.777844928359224E-4 0.364925295343662 >5k 3.2937026695203604E-5 0.1073568579104417 >10k+ 2.19580164158524E-5 3.785597958306068 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1245703 3.493192959117374 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 45805 0.12844610913867216 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 36323 0.10185674101613337 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.11476725049052365 8.412582194433283E-6 0.0 2.804194064811094E-6 5.608388129622188E-6 2 0.11481492178962543 1.6825164388866566E-5 0.0 2.804194064811094E-6 8.412582194433283E-6 3 0.1148794182531161 2.2433552518488752E-5 0.0 2.804194064811094E-6 1.1216776259244376E-5 4 0.11492428535815306 3.084613471292203E-5 0.0 8.412582194433283E-6 1.1216776259244376E-5 5 0.11494671891067156 4.206291097216641E-5 0.0 8.412582194433283E-6 2.5237746583299847E-5 6 0.11498036923944929 4.4867105036977504E-5 0.0 1.1216776259244376E-5 2.8041940648110942E-5 7 0.11501962795635665 5.3279687231410785E-5 0.0 1.1216776259244376E-5 3.084613471292203E-5 8 0.11504766989700475 6.449646349065516E-5 0.0 1.1216776259244376E-5 3.365032877773313E-5 9 0.11509814539017135 7.010485162027735E-5 0.0 1.4020970324055471E-5 3.645452284254422E-5 10-11 0.11518928169727771 7.851743381471064E-5 0.0 1.6825164388866566E-5 3.645452284254422E-5 12-13 0.11530705784799979 9.394050117117166E-5 0.0 1.9629358453677657E-5 3.785661987494977E-5 14-15 0.11545848432749958 1.0796147149522712E-4 0.0 2.2433552518488752E-5 4.346500800457196E-5 16-17 0.11557065209009203 1.247866358840937E-4 0.0 2.5237746583299847E-5 4.7671299101788596E-5 18-19 0.11573609953991588 1.3740550917574362E-4 0.0 2.6639843615705396E-5 4.7671299101788596E-5 20-21 0.1158945365045777 1.612411587266379E-4 0.0 3.224823174532758E-5 5.468178426381634E-5 22-23 0.11615532655260513 1.8367471124512664E-4 0.0 3.365032877773313E-5 5.6083881296221884E-5 24-25 0.11721951820020095 2.1452084595804867E-4 0.0 4.206291097216641E-5 6.169226942584407E-5 26-27 0.12070232722869634 2.355523014441319E-4 0.0 4.4867105036977504E-5 6.589856052306072E-5 28-29 0.13046793305940096 2.495732717681874E-4 0.0 4.4867105036977504E-5 6.730065755546626E-5 30-31 0.15412691838421216 2.734089213190816E-4 0.0 4.4867105036977504E-5 7.010485162027735E-5 32-33 0.20070738599478927 2.860277946107316E-4 0.0 4.4867105036977504E-5 8.132162787952173E-5 34-35 0.2792921204670553 3.042550560320037E-4 0.0 4.7671299101788596E-5 9.394050117117165E-5 36-37 0.3967654182301217 3.210802204208703E-4 0.0 5.0475493166599694E-5 9.674469523598275E-5 38-39 0.5573630144188855 3.365032877773313E-4 0.0 5.187759019900524E-5 1.0095098633319939E-4 40-41 0.770676654832033 3.5332845216619785E-4 0.0 5.468178426381634E-5 1.0235308336560493E-4 42-43 1.0381911602268818 3.701536165550644E-4 0.0 6.029017239343852E-5 1.1076566556003821E-4 44-45 1.354856177092707 3.911850720411476E-4 0.0 6.169226942584407E-5 1.1216776259244377E-4 46-47 1.7284813859000074 4.2203120675406966E-4 0.0 7.010485162027735E-5 1.1497195665725486E-4 48-49 2.1562387569344215 4.4166056520774733E-4 0.0 7.571323974989954E-5 1.1497195665725486E-4 50-51 2.6358400678390628 4.584857295966139E-4 0.0 7.851743381471063E-5 1.2198244181928259E-4 52-53 3.16522704017036 4.7951718508269705E-4 0.0 7.851743381471063E-5 1.2759082994890476E-4 54-55 3.738642663513257 5.005486405687803E-4 0.0 8.412582194433283E-5 1.317971210461214E-4 56-57 4.356330902751392 5.398073574761356E-4 0.0 9.25384041387661E-5 1.3460131511093253E-4 58-59 5.0108774687774025 5.706534921890577E-4 0.0 1.0095098633319939E-4 1.3740550917574362E-4 60-61 5.702423973391563 5.958912387723574E-4 0.0 1.0095098633319939E-4 1.458180913701769E-4 62-63 6.425886412754372 6.12716403161224E-4 0.0 1.0095098633319939E-4 1.4862228543498798E-4 64-65 7.179032648390239 6.309436645824962E-4 0.0 1.0095098633319939E-4 1.5423067356461017E-4 66-67 7.957218934927836 6.561814111657961E-4 0.0 1.0796147149522712E-4 1.5703486762942126E-4 68-69 8.761259890742991 6.702023814898515E-4 0.0 1.1076566556003821E-4 1.6544744982385456E-4 70-71 9.585401309614713 6.898317399435291E-4 0.0 1.1356985962484931E-4 1.7105583795347674E-4 72-73 10.424825586139654 7.080590013648013E-4 0.0 1.1777615072206595E-4 1.8367471124512664E-4 74-75 11.281525100200863 7.248841657536678E-4 0.0 1.191782477544715E-4 1.8647890530993776E-4 76-77 12.151722602393043 7.445135242073455E-4 0.0 1.2058034478687704E-4 1.8928309937474882E-4 78-79 13.034924554560783 7.529261064017788E-4 0.0 1.247866358840937E-4 1.9068519640715438E-4 80-81 13.929538174475272 7.64142882661023E-4 0.0 1.2618873291649922E-4 1.9208729343955994E-4 82-83 14.833648197590243 7.781638529850786E-4 0.0 1.2899292698131032E-4 2.0049987563399324E-4 84-85 15.747328935048415 8.04803696600784E-4 0.0 1.317971210461214E-4 2.1031455486083205E-4 86-87 16.667767780202784 8.510728986701671E-4 0.0 1.317971210461214E-4 2.173250400228598E-4 88 17.358034170226357 8.777127422858724E-4 0.0 1.3740550917574362E-4 2.1872713705526532E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 172060 0.0 79.9826 8 AGTGGTA 173885 0.0 79.16054 5 GTGGTAT 173985 0.0 79.05714 6 GTATCAA 174050 0.0 78.80649 9 TGGTATC 174580 0.0 78.61758 7 AAGCAGT 180425 0.0 77.3046 1 GCAGTGG 187110 0.0 74.44952 3 CAGTGGT 189245 0.0 72.99035 4 AGCAGTG 194530 0.0 71.87827 2 CAACGCA 168725 0.0 43.198708 12-13 ATCAACG 170815 0.0 42.74332 10-11 ACGCAGA 173305 0.0 42.02163 14-15 AGAGTAC 172710 0.0 41.388847 18-19 TCAACGC 168320 0.0 40.843285 12-13 AGTACTT 172220 0.0 40.16904 20-21 GAGTACT 162725 0.0 40.071095 20-21 AACGCAG 171290 0.0 40.001297 14-15 CGCAGAG 175290 0.0 38.99529 16-17 TATCAAC 177275 0.0 38.687984 10-11 GTACTTT 170455 0.0 38.22681 22-23 >>END_MODULE