FastQCFastQC Report
Thu 26 May 2016
SRR1660650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35660870
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13339743.740721973412314No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT492960.1382355506189277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1827050.080.305678
AGTGGTA1851800.079.394765
GTGGTAT1853400.079.3490456
GTATCAA1848300.079.145959
TGGTATC1856400.078.9904567
AAGCAGT1888800.078.251671
GCAGTGG1961750.075.294663
CAGTGGT2006000.073.3948064
AGCAGTG2040000.072.671632
CAACGCA1786900.043.34689712-13
ATCAACG1804650.043.00387610-11
ACGCAGA1830300.042.3883814-15
AGAGTAC1848050.041.54676418-19
TCAACGC1783150.040.89233412-13
AGTACTT1837750.040.4520920-21
AACGCAG1813350.040.11023714-15
GAGTACT1742450.040.10329420-21
CGCAGAG1859450.039.0652716-17
TATCAAC1875250.038.97418610-11
GTACTTT1817850.038.32338722-23
Produced by FastQC (version 0.11.5)