FastQCFastQC Report
Thu 26 May 2016
SRR1660649_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660649_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48054733
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10511282.187355821954104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1757100.0141.01851
GGTATCA3017450.079.310918
GTGGTAT3030350.079.056476
AGTGGTA3041250.078.8498845
TGGTATC3053550.078.244867
GTATCAA3067150.077.7441949
GCAGTGG3156000.076.719553
AGCAGTG3210650.075.684112
CAGTGGT3224100.074.648784
GAGTGTA486700.049.31413394
CAACGCA2990100.042.72196612-13
ATCAACG3014150.042.51138710-11
ACGCAGA3068100.041.8006814-15
AGAGTAC3075950.041.2687918-19
AGTACTT3033900.040.27479620-21
TCAACGC2988500.040.011712-13
GAGTACT2871150.039.7865920-21
AACGCAG3030450.039.26233714-15
GTACTTT2960300.038.50848422-23
TATCAAC3101650.038.38252610-11
Produced by FastQC (version 0.11.5)