FastQCFastQC Report
Thu 26 May 2016
SRR1660649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48054733
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23745874.941421690970586No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1048630.21821575826880568No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC656800.1366774839847721TruSeq Adapter, Index 12 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA651540.13558289877502805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3217700.082.662211
GGTATCA3291800.079.966248
GTGGTAT3320250.079.5922856
AGTGGTA3330550.079.473075
TGGTATC3351000.078.7105267
GTATCAA3344450.078.550089
GCAGTGG3428850.077.50843
CAGTGGT3520350.075.289684
AGCAGTG3543200.075.249572
CAACGCA3266150.043.02404412-13
ATCAACG3284950.042.890610-11
ACGCAGA3346450.042.18301814-15
AGAGTAC3365950.041.61102318-19
AGTACTT3327000.040.73668720-21
TCAACGC3257250.040.1845912-13
GAGTACT3162900.039.90757820-21
AACGCAG3300950.039.5083314-15
TATCAAC3393500.038.7066610-11
GTACTTT3259100.038.6660722-23
TACTTTT3514600.038.52749622-23
Produced by FastQC (version 0.11.5)