FastQCFastQC Report
Thu 26 May 2016
SRR1660648_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660648_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26190794
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8740263.337149687023616No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290720.11100083487350557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1229500.079.751138
GTGGTAT1246350.078.801026
GTATCAA1242650.078.654059
AGTGGTA1254950.078.3171165
TGGTATC1252500.078.196687
AAGCAGT1297950.076.515441
CAGTGGT1368650.072.054524
GCAGTGG1386250.071.773033
AGCAGTG1427850.069.757572
GAGTGTA242950.051.48995694
CAACGCA1208600.042.6070812-13
ATCAACG1223600.042.23766310-11
ACGCAGA1246550.041.45221314-15
AGAGTAC1243750.040.9624118-19
TCAACGC1207600.040.51033812-13
GAGTACT1163650.039.87907420-21
AACGCAG1222800.039.8306214-15
AGTACTT1238000.039.586720-21
GTACTTT1204550.038.3804122-23
TATCAAC1272550.038.3199810-11
Produced by FastQC (version 0.11.5)