FastQCFastQC Report
Thu 26 May 2016
SRR1660648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26190794
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9219403.5200918307402214No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT310290.1184729260212577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1447500.080.6727758
GTATCAA1454150.079.981059
GTGGTAT1473200.079.950496
AGTGGTA1475950.079.865145
TGGTATC1477850.079.311437
AAGCAGT1492150.079.236861
CAGTGGT1597400.074.3601464
GCAGTGG1611100.073.905563
AGCAGTG1639200.072.435312
CAACGCA1422050.043.2917612-13
ATCAACG1438700.042.84416610-11
ACGCAGA1465450.042.13142414-15
AGAGTAC1479800.041.66044218-19
TCAACGC1417950.040.90418612-13
AGTACTT1470950.040.6583920-21
GAGTACT1402200.040.19611720-21
AACGCAG1449550.040.06424314-15
GTACTTT1447000.039.04303722-23
TATCAAC1490800.039.04045510-11
TACTTTT1544300.039.03949722-23
Produced by FastQC (version 0.11.5)