FastQCFastQC Report
Thu 26 May 2016
SRR1660647_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660647_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28585790
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7355042.572970696279515No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT318900.1115589249063958No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT312000.10914513819628563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA304070.10637103260046338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1135950.074.876521
GCAGTGG1214850.069.9162753
AGTGGTA1236500.067.912125
GTGGTAT1236200.067.8438646
AGCAGTG1265650.067.369122
TGGTATC1281300.065.346057
GGTATCA1290750.064.996528
CAGTGGT1304900.064.5881044
GTATCAA1396500.059.9329159
AGTACTT1268500.032.52572620-21
GAGTACT1194650.032.26031520-21
ATCAACG1453150.031.2007410-11
CAACGCA1460350.030.978212-13
GTACTTT1250600.030.76093122-23
TACTTTT1381400.029.9850922-23
ACGCAGA1509800.029.92091814-15
AGAGTAC1499150.029.52397518-19
TCAACGC1447100.028.98627712-13
TATCAAC1473800.028.39088810-11
AACGCAG1489300.028.05012114-15
Produced by FastQC (version 0.11.5)