FastQCFastQC Report
Thu 26 May 2016
SRR1660647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28585790
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7225182.52754253074692No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT345410.12083276341147123No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT333610.11670483831302196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1099300.074.661031
GCAGTGG1182150.069.1855853
GTGGTAT1209000.067.1593556
AGTGGTA1215400.066.894635
AGCAGTG1233800.066.586372
TGGTATC1258800.064.4314967
GGTATCA1265200.064.283858
CAGTGGT1286800.063.4202464
GTATCAA1378250.058.891689
AGTACTT1254800.032.156920-21
GAGTACT1183950.031.73703420-21
ATCAACG1435350.030.51702110-11
CAACGCA1439050.030.40590712-13
GTACTTT1243250.030.17525322-23
TACTTTT1366700.029.67660122-23
ACGCAGA1489700.029.3389814-15
AGAGTAC1495700.028.95066618-19
TCAACGC1424300.028.42122512-13
TATCAAC1463350.027.77839710-11
AACGCAG1469100.027.49055114-15
Produced by FastQC (version 0.11.5)