FastQCFastQC Report
Thu 26 May 2016
SRR1660646_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660646_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33315722
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11043673.3148523691006906No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT371990.11165599232698603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1610250.077.564881
AGTGGTA1589950.077.420395
GGTATCA1592600.077.1529548
GTGGTAT1605400.076.5492256
TGGTATC1612550.076.011837
GTATCAA1615700.075.850879
GCAGTGG1701650.073.3253563
CAGTGGT1712400.072.206614
AGCAGTG1764500.070.936452
CAACGCA1585550.041.222712-13
ATCAACG1597900.041.00025610-11
ACGCAGA1630950.040.0411214-15
AGAGTAC1620950.039.47010418-19
AGTACTT1551350.039.32258220-21
GAGTACT1471800.039.0910820-21
TCAACGC1580900.038.88624212-13
AACGCAG1615450.037.91481814-15
GTACTTT1526200.037.5774622-23
TATCAAC1661650.036.8786410-11
CGCAGAG1672400.036.47531516-17
Produced by FastQC (version 0.11.5)