##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660646_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33315722 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19436667168732 34.0 31.0 34.0 30.0 34.0 2 32.35635322566325 34.0 31.0 34.0 30.0 34.0 3 32.37937788050939 34.0 31.0 34.0 30.0 34.0 4 35.76026636913347 37.0 35.0 37.0 35.0 37.0 5 35.754862764192836 37.0 35.0 37.0 35.0 37.0 6 35.769400134867254 37.0 35.0 37.0 35.0 37.0 7 35.712952881525425 37.0 35.0 37.0 35.0 37.0 8 35.74588907303285 37.0 35.0 37.0 35.0 37.0 9 37.438742615273355 39.0 38.0 39.0 35.0 39.0 10-11 37.33721025166437 39.0 37.0 39.0 34.5 39.0 12-13 37.36215075873187 39.0 38.0 39.0 34.0 39.0 14-15 38.7541373409227 40.5 38.5 41.0 35.0 41.0 16-17 38.68634737977463 40.0 38.0 41.0 34.0 41.0 18-19 38.56603759030046 40.0 38.0 41.0 34.0 41.0 20-21 38.518471849416926 40.0 38.0 41.0 34.0 41.0 22-23 38.37731519070786 40.0 38.0 41.0 34.0 41.0 24-25 38.3417496400048 40.0 38.0 41.0 34.0 41.0 26-27 38.274344917393655 40.0 38.0 41.0 34.0 41.0 28-29 38.16730755527375 40.0 38.0 41.0 33.0 41.0 30-31 37.985404398559936 40.0 38.0 41.0 33.0 41.0 32-33 37.96556649740324 40.0 38.0 41.0 33.0 41.0 34-35 38.009906659084265 40.0 38.0 41.0 33.0 41.0 36-37 37.853673980110656 40.0 38.0 41.0 33.0 41.0 38-39 37.537006146827615 40.0 37.0 41.0 32.0 41.0 40-41 37.37430249898232 40.0 36.5 41.0 31.5 41.0 42-43 37.30562382829344 40.0 36.5 41.0 31.5 41.0 44-45 37.405969604981095 40.0 36.5 41.0 32.0 41.0 46-47 37.28532623426261 40.0 36.0 41.0 31.5 41.0 48-49 37.11145267990891 40.0 36.0 41.0 31.0 41.0 50-51 35.971944146970614 38.5 34.0 40.0 29.5 40.5 52-53 36.03907324896036 38.5 35.0 40.0 29.0 40.5 54-55 36.35204949182851 39.0 35.0 41.0 28.5 41.0 56-57 36.04064594487852 39.0 35.0 41.0 27.5 41.0 58-59 35.77684048089968 39.0 35.0 41.0 27.0 41.0 60-61 35.500060076740944 38.5 35.0 41.0 26.5 41.0 62-63 35.211383382296205 37.5 34.5 40.0 26.0 41.0 64-65 34.89188960095177 37.0 34.0 40.0 26.0 41.0 66-67 34.52583832642138 36.5 34.0 40.0 26.0 41.0 68-69 34.179007842003244 36.0 34.0 39.0 25.5 41.0 70-71 33.84629099438397 35.5 33.0 39.0 25.0 41.0 72-73 33.46928900415246 35.0 33.0 38.0 24.5 40.0 74-75 33.08438763536327 35.0 33.0 37.0 24.0 39.5 76-77 32.711845026201146 35.0 33.0 37.0 23.5 39.0 78-79 32.369765031656826 35.0 32.0 36.0 23.0 39.0 80-81 32.04512635205685 35.0 32.0 36.0 21.5 37.5 82-83 31.72430926155525 35.0 32.0 35.0 20.0 37.0 84-85 31.466730902605082 35.0 31.0 35.0 20.0 36.5 86-87 31.207270729417182 35.0 31.0 35.0 20.0 36.0 88-89 30.99411142883231 34.5 31.0 35.0 19.0 36.0 90-91 30.7653213849005 34.0 31.0 35.0 18.5 35.5 92-93 30.59641183823061 34.0 30.0 35.0 18.0 35.0 94-95 30.404230110936815 34.0 30.0 35.0 18.0 35.0 96-97 30.206555436499322 34.0 30.0 35.0 17.0 35.0 98-99 30.053739927953536 34.0 29.0 35.0 16.5 35.0 100 29.910075099077847 34.0 29.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2533157673665869 1101 2 0.23633165872459472 1101 3 0.2347133466134892 1101 4 0.18265682120947702 1101 5 0.18102820563050415 1101 6 0.1279008689557486 1101 7 0.17798647706960935 1101 8 0.18753734439562209 1101 9 0.24449537179040703 1101 10-11 0.28545354225084196 1101 12-13 0.249471938505458 1101 14-15 0.2858283738719507 1101 16-17 0.2777121793918269 1101 18-19 0.24445173933514042 1101 20-21 0.2895163545021333 1101 22-23 0.2674106549517603 1101 24-25 0.2375738119384394 1101 26-27 0.2496115162646646 1101 28-29 0.19656489748804518 1101 30-31 0.24782712811192908 1101 32-33 0.2085050260655379 1101 34-35 0.18912160683048995 1101 36-37 0.20411990545188985 1101 38-39 0.1983500291548097 1101 40-41 0.1705565321312008 1101 42-43 0.19525820728124188 1101 44-45 0.20955567152516608 1101 46-47 0.232925622269903 1101 48-49 0.2162752944917088 1101 50-51 0.1281417525683537 1101 52-53 0.19561990654266737 1101 54-55 0.3251713748498517 1101 56-57 0.35023296319017305 1101 58-59 0.3091713732145962 1101 60-61 0.2872989995708437 1101 62-63 0.2510828730803709 1101 64-65 0.23746687950325907 1101 66-67 0.2237265372975017 1101 68-69 0.24582717424178213 1101 70-71 0.17501558106935988 1101 72-73 0.17873424025031426 1101 74-75 0.1422234465952883 1101 76-77 0.16803135404861536 1101 78-79 0.1500573409631656 1101 80-81 0.05807307657190819 1101 82-83 0.06209111952823321 1101 84-85 0.020991026639755717 1101 86-87 0.035291729705946295 1101 88-89 0.0034491918582908454 1101 90-91 -0.0036440257912317975 1101 92-93 -0.04390532638662137 1101 94-95 -0.08748181209212902 1101 96-97 -0.04116301124491173 1101 98-99 -0.16136444812980244 1101 100 -0.2278194404817171 1102 1 0.15685991915426456 1102 2 0.17671158680278864 1102 3 0.14103116062614163 1102 4 0.11206921812994608 1102 5 0.12341896183635015 1102 6 0.1305207357851117 1102 7 0.1207442105655332 1102 8 0.14002393305099758 1102 9 0.20095836680567913 1102 10-11 0.21985792327041054 1102 12-13 0.17360221589019176 1102 14-15 0.15981984449004472 1102 16-17 0.17612669070779674 1102 18-19 0.17319362885780265 1102 20-21 0.21594563292043034 1102 22-23 0.20582080051442375 1102 24-25 0.20819879564385246 1102 26-27 0.18910001661642895 1102 28-29 0.1805036417664283 1102 30-31 0.23278383548191073 1102 32-33 0.16482336022861688 1102 34-35 0.15479019525616877 1102 36-37 0.11553260878243776 1102 38-39 0.12663072395706365 1102 40-41 0.11290270059630814 1102 42-43 0.16042568821733738 1102 44-45 0.08728179822442428 1102 46-47 0.16235937567309833 1102 48-49 0.20850242828834098 1102 50-51 0.2181683900138367 1102 52-53 0.22228554546018842 1102 54-55 0.2735543277144288 1102 56-57 0.2846119270435139 1102 58-59 0.21139906577404588 1102 60-61 0.19895575875482763 1102 62-63 0.17623077051270286 1102 64-65 0.19915271924414668 1102 66-67 0.21072705288462856 1102 68-69 0.2175760976979504 1102 70-71 0.12545865668496958 1102 72-73 0.16830752990718167 1102 74-75 0.14371911838535567 1102 76-77 0.19355810287968467 1102 78-79 0.11698024181078637 1102 80-81 0.08110678113840208 1102 82-83 0.06459535660015803 1102 84-85 0.04367148365348683 1102 86-87 0.055411794144607285 1102 88-89 0.01602989933881105 1102 90-91 0.02908069538500513 1102 92-93 -0.0032708564778154425 1102 94-95 -0.06778670735537418 1102 96-97 -0.050679641841963274 1102 98-99 -0.1606226354489877 1102 100 -0.25516582227231766 1103 1 0.06331118711715789 1103 2 0.13884131467796834 1103 3 0.10132651593578856 1103 4 0.12994958119639222 1103 5 0.12144246354711896 1103 6 0.10274184111100482 1103 7 0.10090504546732859 1103 8 0.13544227371113493 1103 9 0.13752817254247418 1103 10-11 0.15680228344765368 1103 12-13 0.1468231739281336 1103 14-15 0.17412102017526365 1103 16-17 0.21037986937489705 1103 18-19 0.21075014705645145 1103 20-21 0.22660901394781519 1103 22-23 0.19128132132976816 1103 24-25 0.17944261692396424 1103 26-27 0.15289335721555375 1103 28-29 0.13258939576009254 1103 30-31 0.20797056858743446 1103 32-33 0.13403012022425287 1103 34-35 0.13794425699550317 1103 36-37 0.13126854978246882 1103 38-39 0.11508364574100938 1103 40-41 0.08458703905672849 1103 42-43 0.14966825945874263 1103 44-45 0.04934895508796444 1103 46-47 0.07167293102463645 1103 48-49 0.11609611159125421 1103 50-51 0.0574200856905378 1103 52-53 0.1724245542368692 1103 54-55 0.22114457095727857 1103 56-57 0.2408210768205663 1103 58-59 0.2308746742167429 1103 60-61 0.2458499139021555 1103 62-63 0.2411073510907329 1103 64-65 0.1227467425435691 1103 66-67 0.18333963533729758 1103 68-69 0.19322054632291952 1103 70-71 0.1653029658352807 1103 72-73 0.2011415563225043 1103 74-75 0.1346838355388016 1103 76-77 0.18974964208042167 1103 78-79 0.19705348187233085 1103 80-81 0.10136429896880372 1103 82-83 0.10231110625101891 1103 84-85 0.08173787567958257 1103 86-87 0.08199288360440349 1103 88-89 0.11579657022881307 1103 90-91 0.10777400652130709 1103 92-93 0.04860499562716214 1103 94-95 -0.009252073445683351 1103 96-97 0.05947551883909341 1103 98-99 -0.06484029403813452 1103 100 -0.10122897873134207 1104 1 0.11269689432410246 1104 2 0.14139675761273907 1104 3 0.12089166397049667 1104 4 0.11813707972800813 1104 5 0.12480099706156977 1104 6 0.08871079127877124 1104 7 0.10876179729507385 1104 8 0.10173619715427407 1104 9 0.12808811634866402 1104 10-11 0.159621568727907 1104 12-13 0.1432194532504738 1104 14-15 0.12493576440370191 1104 16-17 0.10820424294789177 1104 18-19 0.1264552875837026 1104 20-21 0.1211326550362557 1104 22-23 0.13631686362356987 1104 24-25 0.13190222094552695 1104 26-27 0.1371980498715928 1104 28-29 0.10270708092629377 1104 30-31 0.1370550623465192 1104 32-33 0.13471098431895 1104 34-35 0.11474836496675067 1104 36-37 0.1102400879145975 1104 38-39 0.021493357795762336 1104 40-41 0.07851824795525175 1104 42-43 0.1329809486136142 1104 44-45 0.0673328261310644 1104 46-47 0.0892976063777482 1104 48-49 0.11549531627311183 1104 50-51 0.05031470553596762 1104 52-53 0.15033479938264804 1104 54-55 0.1991638855118154 1104 56-57 0.21659902461968983 1104 58-59 0.18043618399032368 1104 60-61 0.2065132511360659 1104 62-63 0.16615900492103464 1104 64-65 0.14361091199215537 1104 66-67 0.14438728100656562 1104 68-69 0.1930098245255536 1104 70-71 0.1861290823829762 1104 72-73 0.14877433216290825 1104 74-75 0.15263360831267647 1104 76-77 0.12052391490875891 1104 78-79 0.08558142580025674 1104 80-81 0.055201925263382634 1104 82-83 0.0719290060686184 1104 84-85 0.07388618811644321 1104 86-87 0.041830970972327464 1104 88-89 0.04859489696241681 1104 90-91 0.03980957324371914 1104 92-93 -0.014854761774042657 1104 94-95 -0.011453593532674944 1104 96-97 -0.020392923080084557 1104 98-99 -0.061110675909382906 1104 100 -0.030585841489006782 1105 1 0.025008878834640313 1105 2 0.039331629185632266 1105 3 0.047601242235863594 1105 4 0.05568036728465131 1105 5 0.04278239724073529 1105 6 0.004432911234509618 1105 7 0.03230373934140829 1105 8 0.05753764106140835 1105 9 0.039775285107168656 1105 10-11 0.04065822211919823 1105 12-13 0.06066949529807175 1105 14-15 0.04937456381629346 1105 16-17 -0.017179550101992902 1105 18-19 0.057865503142238595 1105 20-21 0.020188267885181688 1105 22-23 0.04523942768597777 1105 24-25 0.05850784715619284 1105 26-27 0.04847016058079845 1105 28-29 0.0477584336382364 1105 30-31 0.02173161737399454 1105 32-33 0.023994278443417727 1105 34-35 0.016105223868599694 1105 36-37 0.02053848659411983 1105 38-39 0.0038962515732947622 1105 40-41 -0.03531779354542408 1105 42-43 0.047589245522850376 1105 44-45 0.06214567020084871 1105 46-47 0.030268513488060478 1105 48-49 0.07000103096090982 1105 50-51 0.006933615858699227 1105 52-53 0.07346927636481126 1105 54-55 0.08150989421651644 1105 56-57 0.18391745915874225 1105 58-59 0.11686664872641472 1105 60-61 0.153917302000373 1105 62-63 0.11975482358280232 1105 64-65 0.13489464929611472 1105 66-67 0.12910906371151043 1105 68-69 0.08056204108342513 1105 70-71 0.048365198960929945 1105 72-73 0.06515032602234072 1105 74-75 0.08047604567089905 1105 76-77 0.09799227728547777 1105 78-79 0.08965881118674446 1105 80-81 0.07384090573910385 1105 82-83 0.08622252472508762 1105 84-85 0.08476169653288324 1105 86-87 0.11568364273779608 1105 88-89 0.061914874262686936 1105 90-91 0.07645020371122513 1105 92-93 0.008871153578773772 1105 94-95 -0.01592147983359027 1105 96-97 0.03552204171005613 1105 98-99 -0.026284165577713736 1105 100 -0.11982631085979989 1106 1 -0.0462635912564906 1106 2 -0.03452550708187374 1106 3 -0.014256019931238484 1106 4 -0.010055195066740907 1106 5 -0.0330530275576848 1106 6 -0.04036059256068114 1106 7 0.006234747660947448 1106 8 0.015776398128252822 1106 9 -0.03219200630309871 1106 10-11 0.005368016626256633 1106 12-13 -0.027643430287106696 1106 14-15 -0.045063548898497174 1106 16-17 -0.03901727820944956 1106 18-19 -0.06011982497001611 1106 20-21 -0.02877447702667979 1106 22-23 -0.050909976059585915 1106 24-25 -0.008265806211511517 1106 26-27 -0.06100866005365191 1106 28-29 -0.06165150475904113 1106 30-31 -0.032868027326053095 1106 32-33 -0.04489190440875035 1106 34-35 -0.07404018771187992 1106 36-37 -0.1392281180490258 1106 38-39 -0.14452548591871306 1106 40-41 -0.06666652358853042 1106 42-43 -0.055133651817484974 1106 44-45 -0.0403982638865088 1106 46-47 -0.07357285925495916 1106 48-49 -0.07788368469909557 1106 50-51 -0.2766526913934797 1106 52-53 -0.10179441279930046 1106 54-55 -0.04220124338686304 1106 56-57 -0.027109671226831722 1106 58-59 0.025204438802560958 1106 60-61 0.054570259741652194 1106 62-63 0.011217145257077732 1106 64-65 0.01269346534935778 1106 66-67 0.08686431542538031 1106 68-69 0.05123435822714839 1106 70-71 0.002168650412841089 1106 72-73 -0.09974186129793594 1106 74-75 -0.15715837131717336 1106 76-77 -0.07818391980273987 1106 78-79 -0.09677981520172096 1106 80-81 -0.07629214353801927 1106 82-83 -0.009357075709502283 1106 84-85 -0.04323613005005811 1106 86-87 -0.040006706360831146 1106 88-89 -0.061622582538760184 1106 90-91 -0.08832463338589136 1106 92-93 -0.12227149125204662 1106 94-95 -0.06417611263042389 1106 96-97 -0.056637116718388114 1106 98-99 -0.11609609030186263 1106 100 -0.2266723393067842 1107 1 -0.06838328109279956 1107 2 -0.041315178413455556 1107 3 -0.006343268978767469 1107 4 0.008792074594786925 1107 5 -0.011033826755976861 1107 6 -0.0203355830845382 1107 7 0.0036898908319074053 1107 8 -0.05408343680927885 1107 9 -0.053692597928673536 1107 10-11 0.018908009186894503 1107 12-13 -0.014082076969955892 1107 14-15 -0.0389220445303593 1107 16-17 -0.05825512834751834 1107 18-19 -0.056926552287457355 1107 20-21 0.017584533678054015 1107 22-23 -0.04023739293463535 1107 24-25 -0.05029146725239286 1107 26-27 -0.04487480344761252 1107 28-29 -0.08562608433275898 1107 30-31 -0.05485928451908961 1107 32-33 -0.10079715310821058 1107 34-35 -0.07558149829071681 1107 36-37 -0.0498844679105801 1107 38-39 -0.027777577716953772 1107 40-41 -0.09958611030480569 1107 42-43 -0.07049739559829504 1107 44-45 -0.10824923728264935 1107 46-47 -0.11453746703198675 1107 48-49 -0.19553554156296826 1107 50-51 -0.31298656697056515 1107 52-53 -0.10688218985126952 1107 54-55 -0.025894666002237443 1107 56-57 0.024704589104111108 1107 58-59 0.02272227769741164 1107 60-61 0.07039831667976415 1107 62-63 0.04052098066840415 1107 64-65 0.022923679200189895 1107 66-67 0.02826000305487497 1107 68-69 0.02361088296486713 1107 70-71 -0.0037638215886346416 1107 72-73 -0.02509644744306172 1107 74-75 -0.001595456400202977 1107 76-77 0.04844553093054316 1107 78-79 0.09901848502827448 1107 80-81 0.02599474028722426 1107 82-83 0.0038495359620789316 1107 84-85 -0.028536271735990937 1107 86-87 0.03587334125733932 1107 88-89 0.028139211870598757 1107 90-91 0.06042080600501265 1107 92-93 -0.06096228930588765 1107 94-95 -0.1055931949762936 1107 96-97 -0.01726124804955731 1107 98-99 -0.10618934820830361 1107 100 -0.14957734392416455 1108 1 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-1.1629535707468222 2316 52-53 -1.3670066923511186 2316 54-55 -1.4149968292756014 2316 56-57 -1.497978663536145 2316 58-59 -1.6228842053956782 2316 60-61 -1.220123213775203 2316 62-63 -1.015349627923321 2316 64-65 -1.1578179625108547 2316 66-67 -1.2561618505611563 2316 68-69 -1.1708751286624732 2316 70-71 -0.9541892197711093 2316 72-73 -0.989683632882894 2316 74-75 -1.0449303613813186 2316 76-77 -1.4023129979236124 2316 78-79 -1.1720841935466453 2316 80-81 -1.0849614507831085 2316 82-83 -1.3772001365558957 2316 84-85 -1.0341531281027834 2316 86-87 -1.001767237320042 2316 88-89 -1.361256689913244 2316 90-91 -1.5753249084455838 2316 92-93 -1.1738887361350088 2316 94-95 -1.0073322937801912 2316 96-97 -1.6351775391987324 2316 98-99 -1.629940514975793 2316 100 -1.2498022787143803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 616.0 3 1417.0 4 2328.0 5 7502.0 6 7061.0 7 9827.0 8 22066.0 9 29209.0 10 32963.0 11 33651.0 12 36212.0 13 40821.0 14 48896.0 15 61032.0 16 78967.0 17 100690.0 18 111804.0 19 124738.0 20 142616.0 21 171566.0 22 215693.0 23 302806.0 24 412859.0 25 490219.0 26 493757.0 27 454870.0 28 442338.0 29 481444.0 30 565895.0 31 689070.0 32 867470.0 33 1130121.0 34 1575499.0 35 2421962.0 36 4213142.0 37 7549895.0 38 7933894.0 39 2006872.0 40 3934.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.667516543581012 29.418540358178753 27.803477824424842 25.11046527381539 2 22.952059126802713 31.80268903079918 24.875225317438094 20.37002652496001 3 25.826722114321942 24.103986329988967 26.605963901381184 23.46332765430791 4 20.67156011941582 27.083467237577825 23.38349219968061 28.861480443325743 5 23.598784204018276 29.43826067363018 25.755212733827154 21.20774238852439 6 28.723060949096098 27.429981102228325 24.007419698879655 19.83953824979593 7 23.012046210743307 24.814093606666884 29.482443061213782 22.691417121376027 8 27.001469848298182 26.577790563997777 24.31240931267737 22.10833027502667 9 25.642226988019388 24.4534441489234 26.894551454760784 23.009777408296426 10-11 22.566211464800364 28.076915468884057 26.968935850236424 22.387937216079155 12-13 22.353339809824575 26.207367900058177 27.00960401704752 24.42968827306973 14-15 22.36326268706869 29.971487938138463 25.260854035664543 22.404395339128303 16-17 24.474338506422345 25.823064045366344 25.365323098665037 24.337274349546277 18-19 22.366444108878188 27.8330211966296 25.47577332228243 24.32476137220978 20-21 24.315364795265186 28.00519830462082 25.450317253317696 22.229119646796306 22-23 24.30443655465865 26.011184424919804 27.337607459768932 22.346771560652616 24-25 22.47709576580713 27.646969477763943 25.372004537492177 24.50393021893675 26-27 22.53053592191355 25.803113756318393 29.340354552390902 22.325995769377162 28-29 22.605687527943058 25.841765367634732 29.240201791294528 22.312345313127683 30-31 22.469873515701448 25.745211275918383 29.326659524419085 22.458255683961088 32-33 22.54485608091958 25.955113335457163 29.113468787339507 22.386561796283747 34-35 22.54303504111657 26.09432582002703 28.982677895721327 22.379961243135075 36-37 22.458965559912183 26.041358467759895 29.115756940121017 22.383919032206904 38-39 22.524907729079153 26.0223984891424 29.148404889633568 22.304288892144875 40-41 22.556286443498745 26.064383845532102 29.08562338911247 22.293706321856682 42-43 22.579441048061433 26.05989377814199 29.075890803957606 22.284774369838974 44-45 22.672797083479857 26.170350798676704 28.99109444546482 22.165757672378618 46-47 22.709092652464314 26.24190545229436 28.88208157648319 22.16692031875813 48-49 22.77353107478883 26.242247742628088 28.802710438455897 22.181510744127188 50-51 22.91561442271866 26.3084812620904 28.685528035383783 22.090376279807153 52-53 23.08874795159688 26.394737797189705 28.43320535370496 22.08330889750846 54-55 23.30645433167788 26.584781306351392 27.925574444275046 22.183189917695685 56-57 23.70640406093262 26.958936265584743 27.191849588700084 22.142810084782553 58-59 23.936850343169972 27.255017912299817 26.740624267888887 22.067507476641328 60-61 24.10609026674077 27.387579975866768 26.528270488818972 21.978059268573485 62-63 24.25987116619993 27.533258929678027 26.380542097080394 21.826327807041647 64-65 24.451477074054452 27.536152089159106 26.291644153782407 21.720726683004038 66-67 24.59055222284269 27.42830774073945 26.39825107272814 21.58288896368972 68-69 24.75419483131019 27.364429936077617 26.473133441881686 21.408241790730507 70-71 24.940349903663385 27.257045360815233 26.522269391834406 21.28033534368697 72-73 25.095191693982997 27.202937151363237 26.526817671497266 21.175053483156496 74-75 25.378745015049713 27.239603268813944 26.39004916986498 20.991602546271356 76-77 25.6681957042706 27.289245853672522 26.123106606651238 20.91945183540564 78-79 25.890696411093405 27.33201725851993 25.93180715796103 20.84547917242564 80-81 26.146623916851404 27.418028036603253 25.68780756681106 20.74754047973428 82-83 26.331243761580108 27.44453438322428 25.50268604925329 20.721535805942327 84-85 26.47493547334076 27.40807290178698 25.41142933527193 20.705562289600334 86-87 26.664228063350798 27.409354350097193 25.308892007135807 20.617525579416203 88-89 26.807410955323302 27.357013089200215 25.240099993663733 20.595475961812753 90-91 26.91524092131157 27.312239581388503 25.216319566069945 20.556199931229973 92-93 27.009748911952997 27.34661062588335 25.186168883504234 20.457471578659415 94-95 27.095453607007425 27.36289662246165 25.115688150144905 20.425961620386023 96-97 27.1051190151191 27.396541977633753 25.08559566956942 20.412743337677718 98-99 27.196082162580947 27.489843924495673 25.004168171150777 20.309905741772607 100 27.202497434950235 27.56100475107182 24.931219695253905 20.305278118724043 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 20531.0 1 15415.0 2 8909.5 3 7474.5 4 7693.5 5 8280.5 6 9028.0 7 9888.0 8 10107.0 9 10147.5 10 10540.0 11 13033.0 12 76391.0 13 94215.0 14 50862.5 15 54365.5 16 61591.0 17 68446.5 18 75254.0 19 82362.5 20 90674.0 21 102266.5 22 117996.5 23 136277.0 24 156328.5 25 179305.5 26 206288.5 27 239258.5 28 277134.0 29 315247.5 30 353120.0 31 392162.0 32 434459.5 33 480032.5 34 525744.0 35 574652.0 36 631368.0 37 683826.5 38 731886.0 39 780044.0 40 817566.5 41 856535.5 42 903373.5 43 943628.5 44 986934.0 45 1040438.0 46 1095476.5 47 1150823.5 48 1201417.5 49 1244387.5 50 1276982.5 51 1298287.0 52 1308472.5 53 1300805.0 54 1271680.5 55 1225722.0 56 1161820.5 57 1074669.0 58 969828.5 59 849698.5 60 712159.0 61 579870.0 62 464576.5 63 359640.5 64 273644.0 65 205960.5 66 151053.0 67 111588.5 68 84268.0 69 64496.5 70 51183.0 71 42684.0 72 36300.0 73 28222.0 74 20383.0 75 15447.5 76 11936.5 77 8504.5 78 5989.0 79 4348.0 80 3051.0 81 2235.0 82 1632.5 83 1152.0 84 850.0 85 667.0 86 535.0 87 425.0 88 366.0 89 342.5 90 276.5 91 213.5 92 174.0 93 149.0 94 136.0 95 116.5 96 85.0 97 60.5 98 44.5 99 34.5 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08098278644539056 2 0.03148063247736309 3 0.021581402318100747 4 0.01568928927909772 5 0.01165515788611755 6 0.011580118239670747 7 0.013008873108017889 8 0.006243298584374068 9 0.0016628785652611701 10-11 0.0022782036661249602 12-13 0.00570751550874389 14-15 0.052103028113873684 16-17 0.0545583253456131 18-19 0.09112664585206948 20-21 0.11044335164040568 22-23 0.10927873632755129 24-25 0.11366555405883144 26-27 0.11157044713003669 28-29 0.10803007661067648 30-31 0.09209315649830431 32-33 0.08984947106954488 34-35 0.11175054228150902 36-37 0.11315978684177999 38-39 0.10035051919331059 40-41 0.08397686833861803 42-43 0.08386580966187676 44-45 0.08189526854618369 46-47 0.078131279880412 48-49 0.08307189020246958 50-51 0.08621905297444853 52-53 0.09382357074536761 54-55 0.10385487068237632 56-57 0.10858086761559602 58-59 0.10325755509665976 60-61 0.0927955275890464 62-63 0.09997382016814764 64-65 0.09883922071387197 66-67 0.09537989301267431 68-69 0.09862610811796305 70-71 0.10433962679842268 72-73 0.12234463956686877 74-75 0.13305129632189872 76-77 0.12823675260587178 78-79 0.1193175402292047 80-81 0.11708886272973464 82-83 0.10523259859113963 84-85 0.11000061772636954 86-87 0.11549201905334665 88-89 0.11710236986609504 90-91 0.12321660025858061 92-93 0.12778651472719096 94-95 0.1426158496580083 96-97 0.14488054618777285 98-99 0.1372775292097827 100 0.13923456318911534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.3315722E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.23489366118429 #Duplication Level Percentage of deduplicated Percentage of total 1 74.72093366293426 37.53598156821908 2 13.164203486747372 13.226047245818911 3 4.736661736948408 7.138370959938113 4 2.26552261446056 4.552331504977378 5 1.29460543212023 3.2517183107875645 6 0.8158343096127026 2.459000987312389 7 0.5529331563127486 1.9443576816379746 8 0.40212877960932264 1.616071718542091 9 0.3003803304265291 1.358061656119928 >10 1.5709448464403284 14.32015069569691 >50 0.12158173840277338 4.205458758811338 >100 0.05323100022889023 4.203357140736781 >500 6.057312212821209E-4 0.19833246298515014 >1k 3.8550425204420745E-4 0.3663065425931587 >5k 2.3835141595199116E-5 0.08002228108725772 >10k+ 2.383514075510828E-5 3.5444304847360484 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1104367 3.3148523691006906 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 37199 0.11165599232698603 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02627888418567066 3.0015858578721484E-6 0.0 3.0015858578721484E-6 6.003171715744297E-6 2 0.026425961892706393 3.0015858578721484E-6 0.0 3.0015858578721484E-6 9.004757573616444E-6 3 0.02653401898358979 9.004757573616444E-6 0.0 3.0015858578721484E-6 1.5007929289360741E-5 4 0.026789153781508923 1.800951514723289E-5 0.0 6.003171715744297E-6 2.1011101005105036E-5 5 0.026810164882514028 3.0015858578721482E-5 0.0 6.003171715744297E-6 2.4012686862977187E-5 6 0.02695424100369189 3.0015858578721482E-5 0.0 6.003171715744297E-6 2.4012686862977187E-5 7 0.026987258448128486 3.902061615233793E-5 0.0 6.003171715744297E-6 3.0015858578721482E-5 8 0.027017274306707206 4.502378786808222E-5 0.0 1.2006343431488593E-5 3.601903029446578E-5 9 0.027086310781438264 5.102695958382652E-5 0.0 1.2006343431488593E-5 3.601903029446578E-5 10-11 0.027165852806671876 6.303330301531511E-5 0.0 1.2006343431488593E-5 4.352299493914615E-5 12-13 0.027303925756133995 6.903647473105942E-5 0.0 1.2006343431488593E-5 4.65245807970183E-5 14-15 0.027429992362164625 7.954202523361193E-5 1.5007929289360742E-6 1.2006343431488593E-5 5.552933837063474E-5 16-17 0.027577070069200363 9.905233330978088E-5 3.0015858578721484E-6 1.3507136360424667E-5 6.153251008637904E-5 18-19 0.027731651740880776 1.1255946967020556E-4 4.502378786808223E-6 1.800951514723289E-5 7.053726765999548E-5 20-21 0.027835206452977365 1.275673989595663E-4 6.003171715744297E-6 1.800951514723289E-5 7.654043937573978E-5 22-23 0.028190894377135216 1.440761211778631E-4 6.003171715744297E-6 1.951030807616896E-5 8.554519694935622E-5 24-25 0.029471070745517688 1.5458167168041565E-4 6.003171715744297E-6 2.1011101005105036E-5 9.454995452297267E-5 26-27 0.0339524384313208 1.7709356561445674E-4 6.003171715744297E-6 2.1011101005105036E-5 1.0055312623871696E-4 28-29 0.0472029392008974 1.8909990904594534E-4 6.003171715744297E-6 2.1011101005105036E-5 1.0505550502552519E-4 30-31 0.07884865890044346 1.9510308076168962E-4 6.003171715744297E-6 2.1011101005105036E-5 1.21564227243822E-4 32-33 0.14038116898682249 2.0710942419317822E-4 6.003171715744297E-6 2.1011101005105036E-5 1.5308087875147956E-4 34-35 0.24384133112888864 2.1761497469573074E-4 6.003171715744297E-6 2.4012686862977187E-5 1.5908405046722386E-4 36-37 0.3949351600424569 2.3562448984296364E-4 6.003171715744297E-6 2.7014272720849334E-5 1.8159594440126498E-4 38-39 0.599298133175682 2.491316262033883E-4 6.003171715744297E-6 3.151665150765756E-5 1.906007019748814E-4 40-41 0.8605066400782189 2.686419342795573E-4 6.003171715744297E-6 3.601903029446578E-5 2.0560863126424213E-4 42-43 1.1756686527760076 2.866514494267901E-4 6.003171715744297E-6 4.202220201021007E-5 2.2211735348253895E-4 44-45 1.5487972915610233 2.896530352846623E-4 6.003171715744297E-6 4.952616665489045E-5 2.3412369691402755E-4 46-47 1.9828506192961992 3.0916334336083124E-4 6.003171715744297E-6 5.252775251276259E-5 2.43128454487644E-4 48-49 2.475623971168927 3.3167523729487236E-4 6.003171715744297E-6 5.402854544169867E-5 2.5663559084806867E-4 50-51 3.0215689157209322 3.3917920193955275E-4 6.003171715744297E-6 5.402854544169867E-5 2.686419342795573E-4 52-53 3.6173026656903913 3.481839595131692E-4 9.004757573616444E-6 5.402854544169867E-5 2.941554140714705E-4 54-55 4.2564708638161886 3.481839595131692E-4 9.004757573616444E-6 5.703013129957081E-5 3.1066413628976733E-4 56-57 4.939894443830454 3.571887170867856E-4 9.004757573616444E-6 6.303330301531511E-5 3.1966889386338376E-4 58-59 5.669278306500457 3.84202989807635E-4 1.2006343431488593E-5 6.303330301531511E-5 3.3017444436593633E-4 60-61 6.435362559454663 4.172204342442286E-4 1.2006343431488593E-5 6.453409594425119E-5 3.4368158072636097E-4 62-63 7.228870801599317 4.322283635335893E-4 1.2006343431488593E-5 7.053726765999548E-5 3.6319188880252996E-4 64-65 8.050179731959583 4.472362928229501E-4 1.5007929289360741E-5 7.50396464468037E-5 3.721966463761464E-4 66-67 8.898286820858932 4.592426362544387E-4 1.5007929289360741E-5 8.1042818162548E-5 3.781998180918907E-4 68-69 9.770301541116233 4.65245807970183E-4 1.800951514723289E-5 8.1042818162548E-5 3.84202989807635E-4 70-71 10.664672072842965 4.787529443306076E-4 2.1011101005105036E-5 8.70459898782923E-5 3.887053685944432E-4 72-73 11.574940504065918 4.86256908975288E-4 2.1011101005105036E-5 9.605074745190875E-5 3.9921091909699566E-4 74-75 12.49896370248257 5.027656311935849E-4 2.1011101005105036E-5 1.0205391916765304E-4 4.0671488374167605E-4 76-77 13.436412994441483 5.07268009980393E-4 2.1011101005105036E-5 1.0205391916765304E-4 4.112172625284843E-4 78-79 14.386557493786267 5.192743534118817E-4 2.1011101005105036E-5 1.0805709088339734E-4 4.127180554574204E-4 80-81 15.34433652676055 5.282791109854981E-4 2.1011101005105036E-5 1.2006343431488593E-4 4.2472439888890896E-4 82-83 16.30797015295061 5.432870402748588E-4 2.4012686862977187E-5 1.2306502017275808E-4 4.352299493914615E-4 84-85 17.279751583951864 5.597957624931556E-4 2.4012686862977187E-5 1.2306502017275808E-4 4.4873708575188617E-4 86-87 18.25403333597273 5.808068634982606E-4 2.5513479791913262E-5 1.2606660603063023E-4 4.697481867569912E-4 88 18.988392327202153 6.093219291480461E-4 2.7014272720849334E-5 1.2606660603063023E-4 4.802537372595437E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 161025 0.0 77.56488 1 AGTGGTA 158995 0.0 77.42039 5 GGTATCA 159260 0.0 77.152954 8 GTGGTAT 160540 0.0 76.549225 6 TGGTATC 161255 0.0 76.01183 7 GTATCAA 161570 0.0 75.85087 9 GCAGTGG 170165 0.0 73.325356 3 CAGTGGT 171240 0.0 72.20661 4 AGCAGTG 176450 0.0 70.93645 2 CAACGCA 158555 0.0 41.2227 12-13 ATCAACG 159790 0.0 41.000256 10-11 ACGCAGA 163095 0.0 40.04112 14-15 AGAGTAC 162095 0.0 39.470104 18-19 AGTACTT 155135 0.0 39.322582 20-21 GAGTACT 147180 0.0 39.09108 20-21 TCAACGC 158090 0.0 38.886242 12-13 AACGCAG 161545 0.0 37.914818 14-15 GTACTTT 152620 0.0 37.57746 22-23 TATCAAC 166165 0.0 36.87864 10-11 CGCAGAG 167240 0.0 36.475315 16-17 >>END_MODULE