FastQCFastQC Report
Thu 26 May 2016
SRR1660646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33315722
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11561903.470403553013199No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT390150.11710687224488187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1667600.078.013551
AGTGGTA1658000.077.746025
GGTATCA1656750.077.742278
GTGGTAT1669100.077.1614156
TGGTATC1679200.076.551787
GTATCAA1686150.076.200029
GCAGTGG1754950.073.882143
CAGTGGT1786300.072.393454
AGCAGTG1831750.071.187242
CAACGCA1653850.041.26778412-13
ATCAACG1664050.041.02188510-11
ACGCAGA1705500.040.03592314-15
AGAGTAC1701650.039.6735118-19
AGTACTT1622100.039.6597520-21
GAGTACT1545450.039.27589820-21
TCAACGC1649650.038.8831912-13
AACGCAG1687250.037.93313214-15
GTACTTT1597850.037.85739522-23
TATCAAC1733300.037.12595410-11
TACTTTT1753950.036.75668722-23
Produced by FastQC (version 0.11.5)