FastQCFastQC Report
Thu 26 May 2016
SRR1660645_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660645_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40119832
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7259481.809449251931065No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT785740.19584827772957775No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT539080.13436746195746782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1231800.070.2505651
GCAGTGG1381250.062.440493
AGCAGTG1420250.061.0008962
GTGGTAT1545200.055.1936386
AGTGGTA1556000.054.8325545
CAGTGGT1631300.052.5384374
TGGTATC1659600.051.275927
GGTATCA1680800.050.7002838
GTATCAA1901750.044.7710769
AGTACTT1700200.024.69620120-21
GAGTACT1594050.023.83730720-21
TACTTTT1825350.023.11626422-23
GTACTTT1661650.022.8986522-23
ATCAACG2127350.022.57066710-11
CAACGCA2171800.022.06131412-13
ACGCAGA2246050.021.30361614-15
AGAGTAC2222200.021.03170818-19
TCAACGC2134100.020.01925512-13
TATCAAC2138000.019.94553410-11
ACTTTTT1974750.019.52078424-25
Produced by FastQC (version 0.11.5)