##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660645_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40119832 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2650362543891 34.0 31.0 34.0 30.0 34.0 2 32.4181490590489 34.0 31.0 34.0 30.0 34.0 3 32.45108927674473 34.0 31.0 34.0 30.0 34.0 4 35.838229681520104 37.0 35.0 37.0 35.0 37.0 5 35.85165880056527 37.0 35.0 37.0 35.0 37.0 6 35.861885662930995 37.0 36.0 37.0 35.0 37.0 7 35.823807961110106 37.0 35.0 37.0 35.0 37.0 8 35.84399717825339 37.0 36.0 37.0 35.0 37.0 9 37.54948288417559 39.0 38.0 39.0 35.0 39.0 10-11 37.482116101582875 39.0 38.0 39.0 35.0 39.0 12-13 37.46682125937117 39.0 38.0 39.0 35.0 39.0 14-15 38.837273084792585 40.5 38.5 41.0 35.0 41.0 16-17 38.77922405058925 40.0 38.0 41.0 35.0 41.0 18-19 38.67622928979364 40.0 38.5 41.0 34.0 41.0 20-21 38.64720449228202 40.0 38.0 41.0 34.0 41.0 22-23 38.559011488383106 40.0 38.0 41.0 34.0 41.0 24-25 38.5060154539032 40.0 38.0 41.0 34.0 41.0 26-27 38.40482223355272 40.0 38.0 41.0 34.0 41.0 28-29 38.27399026745675 40.0 38.0 41.0 34.0 41.0 30-31 38.06643781559205 40.0 38.0 41.0 33.0 41.0 32-33 38.02660198078596 40.0 38.0 41.0 33.0 41.0 34-35 38.054596390134435 40.0 38.0 41.0 33.0 41.0 36-37 37.903696942200554 40.0 38.0 41.0 33.0 41.0 38-39 37.66083530708703 40.0 37.0 41.0 33.0 41.0 40-41 37.491413286576076 40.0 37.0 41.0 32.0 41.0 42-43 37.41150153619786 40.0 36.5 41.0 31.5 41.0 44-45 37.483494434872014 40.0 37.0 41.0 32.0 41.0 46-47 37.32442154792672 40.0 36.5 41.0 31.5 41.0 48-49 37.12284058168538 40.0 36.0 41.0 31.0 41.0 50-51 35.95808132995173 38.5 34.5 40.0 29.5 40.5 52-53 36.04343081496452 38.5 35.0 40.0 29.5 40.5 54-55 36.47512783702584 39.0 35.0 41.0 29.5 41.0 56-57 36.26424619624529 39.0 35.0 41.0 28.5 41.0 58-59 36.02083235293707 39.0 35.0 41.0 28.0 41.0 60-61 35.74268412938518 38.5 35.0 41.0 28.0 41.0 62-63 35.4504784566396 38.0 35.0 40.0 27.0 41.0 64-65 35.12214181255794 37.0 34.0 40.0 27.0 41.0 66-67 34.736350640750445 36.5 34.0 40.0 26.0 41.0 68-69 34.36305034876517 36.0 34.0 39.0 26.0 41.0 70-71 34.004082507125155 35.5 33.5 39.0 26.0 41.0 72-73 33.608604330646244 35.0 33.0 38.0 25.5 40.0 74-75 33.21186285127017 35.0 33.0 37.0 25.0 39.5 76-77 32.83672689606477 35.0 33.0 37.0 24.0 39.0 78-79 32.49249256577146 35.0 32.5 36.0 24.0 39.0 80-81 32.16786286642477 35.0 32.0 36.0 23.0 37.5 82-83 31.849513103644103 35.0 32.0 35.0 22.5 37.0 84-85 31.592979464619894 35.0 31.5 35.0 21.0 36.5 86-87 31.333447620119646 35.0 31.0 35.0 20.0 36.0 88-89 31.12100024496613 34.5 31.0 35.0 20.0 36.0 90-91 30.89511329459206 34.0 31.0 35.0 19.5 35.5 92-93 30.727247125561245 34.0 30.5 35.0 19.0 35.0 94-95 30.53226379412556 34.0 30.0 35.0 18.5 35.0 96-97 30.324437811703696 34.0 30.0 35.0 18.0 35.0 98-99 30.15021314147078 34.0 29.5 35.0 17.0 35.0 100 29.9817221816881 34.0 29.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23056068346019742 1101 2 0.23531439888069627 1101 3 0.23376447257665944 1101 4 0.18383995910080841 1101 5 0.17896194078416272 1101 6 0.14323021823407345 1101 7 0.17453681774895102 1101 8 0.1733564247819288 1101 9 0.2503963894299588 1101 10-11 0.27853516896969666 1101 12-13 0.24664904399656962 1101 14-15 0.307327610757703 1101 16-17 0.27460839847123 1101 18-19 0.26712753414568624 1101 20-21 0.3099240131409786 1101 22-23 0.27688770768330784 1101 24-25 0.2536873822657242 1101 26-27 0.21671088967966057 1101 28-29 0.19765425063719277 1101 30-31 0.2589655546803371 1101 32-33 0.21577114219621052 1101 34-35 0.1981888583729372 1101 36-37 0.17733606388039647 1101 38-39 0.15869645684639266 1101 40-41 0.1777693124095734 1101 42-43 0.2200436382853681 1101 44-45 0.24252547552048753 1101 46-47 0.26682171478499583 1101 48-49 0.24397108313158355 1101 50-51 0.1493323482969373 1101 52-53 0.22100767087697903 1101 54-55 0.30502296336597823 1101 56-57 0.31687405727353024 1101 58-59 0.2761057830154243 1101 60-61 0.283943642861999 1101 62-63 0.2641109812998437 1101 64-65 0.22277796341963096 1101 66-67 0.25137897214040805 1101 68-69 0.23743704029210022 1101 70-71 0.13732119725608527 1101 72-73 0.1422180168126772 1101 74-75 0.12321411920567016 1101 76-77 0.1820056734932507 1101 78-79 0.1379747291239326 1101 80-81 0.09448474939915741 1101 82-83 0.015524260995302797 1101 84-85 -0.004474532705412315 1101 86-87 -0.02705836459088573 1101 88-89 -0.06576300908174204 1101 90-91 -0.07095195674640209 1101 92-93 -0.12824972884547492 1101 94-95 -0.17656087364589368 1101 96-97 -0.12126509055026347 1101 98-99 -0.21321299549545358 1101 100 -0.3014244851217036 1102 1 0.14132018657151946 1102 2 0.1823353466144937 1102 3 0.14530771184545443 1102 4 0.10566746263308602 1102 5 0.14096511454213356 1102 6 0.16522908258448865 1102 7 0.14137213474048593 1102 8 0.15420808047279877 1102 9 0.19567617578023544 1102 10-11 0.2054284669222355 1102 12-13 0.16598959794542623 1102 14-15 0.18242169132506802 1102 16-17 0.21437876903900133 1102 18-19 0.21033770943935082 1102 20-21 0.24912216805972065 1102 22-23 0.20594668999590482 1102 24-25 0.2136233570455346 1102 26-27 0.19152128247110767 1102 28-29 0.18708346001594833 1102 30-31 0.21422367145502363 1102 32-33 0.1576537296545979 1102 34-35 0.12830576324687826 1102 36-37 0.129291394689254 1102 38-39 0.09782639104962954 1102 40-41 0.10323587283215119 1102 42-43 0.15977313783838554 1102 44-45 0.1074745393902532 1102 46-47 0.1673685586214404 1102 48-49 0.22384273586235537 1102 50-51 0.18421106348176863 1102 52-53 0.21604265173531445 1102 54-55 0.24538149268504839 1102 56-57 0.25911988829287935 1102 58-59 0.23263494457386003 1102 60-61 0.20714618768584359 1102 62-63 0.17390715117073796 1102 64-65 0.15908115973656578 1102 66-67 0.19729348022786297 1102 68-69 0.19923092275200815 1102 70-71 0.11670150450736827 1102 72-73 0.1115569818321589 1102 74-75 0.0608371779908623 1102 76-77 0.10325915211537051 1102 78-79 0.08873075041092449 1102 80-81 0.07671801830418445 1102 82-83 0.04683595919697581 1102 84-85 -0.011306594434838502 1102 86-87 0.016863602109232545 1102 88-89 -0.02510148225083597 1102 90-91 -0.06820075834433936 1102 92-93 -0.10000175341798823 1102 94-95 -0.17040358540324263 1102 96-97 -0.13030271927682335 1102 98-99 -0.21110521517852732 1102 100 -0.2963995026481925 1103 1 0.07853025133111657 1103 2 0.13349115281486235 1103 3 0.09320773205860178 1103 4 0.1485731095036158 1103 5 0.1139798991219152 1103 6 0.09796865706042723 1103 7 0.1014846033309098 1103 8 0.1337734938761841 1103 9 0.14803276764170903 1103 10-11 0.15748273417644754 1103 12-13 0.1465780112745847 1103 14-15 0.1667534398669659 1103 16-17 0.2069795469701461 1103 18-19 0.21079315609348015 1103 20-21 0.20616019882765357 1103 22-23 0.17874591406256712 1103 24-25 0.14339006034828117 1103 26-27 0.16197232164525843 1103 28-29 0.1660332132003859 1103 30-31 0.2068581786801431 1103 32-33 0.15368967307771442 1103 34-35 0.1322151691561615 1103 36-37 0.13956343828998996 1103 38-39 0.1530733618039548 1103 40-41 0.10686605196646326 1103 42-43 0.15637405759678558 1103 44-45 0.0601988926742294 1103 46-47 0.13047936264123905 1103 48-49 0.15576097322522742 1103 50-51 0.07563258858083799 1103 52-53 0.15333993379180555 1103 54-55 0.2527022001901784 1103 56-57 0.27188243523956857 1103 58-59 0.2252463144228969 1103 60-61 0.2285178711605127 1103 62-63 0.24877408299612824 1103 64-65 0.15388014463159294 1103 66-67 0.21258515238810816 1103 68-69 0.22067181055621887 1103 70-71 0.1262357472311919 1103 72-73 0.16452403344023026 1103 74-75 0.13099455760801249 1103 76-77 0.17474717800549655 1103 78-79 0.1963326939802883 1103 80-81 0.07903220988984572 1103 82-83 0.06650870539965581 1103 84-85 0.03451007791167271 1103 86-87 0.060341429261402624 1103 88-89 0.10370993659356742 1103 90-91 0.09979922884037506 1103 92-93 0.04921921693317799 1103 94-95 -0.012268887127781625 1103 96-97 0.0587409899996878 1103 98-99 -0.019295938733179696 1103 100 -0.1037091566587165 1104 1 0.08398912115185908 1104 2 0.11157773130964443 1104 3 0.10398197157025635 1104 4 0.10067516199183046 1104 5 0.09260693035731293 1104 6 0.055370266381345346 1104 7 0.08111866012986724 1104 8 0.06746217946652422 1104 9 0.07200630119754692 1104 10-11 0.10901101990967987 1104 12-13 0.11345363952921161 1104 14-15 0.09994168278039695 1104 16-17 0.09392108866190085 1104 18-19 0.08391672462462196 1104 20-21 0.09085016145694169 1104 22-23 0.09336497056812476 1104 24-25 0.11063761544351536 1104 26-27 0.1104525828553875 1104 28-29 0.08150622119167394 1104 30-31 0.10457686895075824 1104 32-33 0.06265568472080218 1104 34-35 0.05780701160805535 1104 36-37 0.04258273993016104 1104 38-39 -0.041111511017440705 1104 40-41 0.020171454237264186 1104 42-43 0.038074353669180994 1104 44-45 -0.007173483222466359 1104 46-47 0.033681717335824146 1104 48-49 0.03426634986715271 1104 50-51 -0.04537555302556484 1104 52-53 0.060734841349130875 1104 54-55 0.09910603571633914 1104 56-57 0.1144643330659818 1104 58-59 0.11743530428088889 1104 60-61 0.16964511434224505 1104 62-63 0.15231210508642334 1104 64-65 0.15335702941208496 1104 66-67 0.149248915043799 1104 68-69 0.15558918483962714 1104 70-71 0.1365429879493547 1104 72-73 0.06788494788224142 1104 74-75 0.09249124266358422 1104 76-77 0.10558920886106193 1104 78-79 0.10138536607072979 1104 80-81 0.05821483919705628 1104 82-83 0.043391278117191234 1104 84-85 0.030123916236355797 1104 86-87 -0.006411367266466073 1104 88-89 -0.005524090528744807 1104 90-91 0.017933339831095907 1104 92-93 -0.05120866633476595 1104 94-95 -0.028775316311776322 1104 96-97 -0.05390988721290668 1104 98-99 -0.15333668327402705 1104 100 -0.19195324826008786 1105 1 0.06952473142167293 1105 2 0.08452887031522494 1105 3 0.0889729955837879 1105 4 0.09147058821024956 1105 5 0.10362160587455094 1105 6 0.050832077597988246 1105 7 0.10250545409157752 1105 8 0.08731278021911493 1105 9 0.09007708189854213 1105 10-11 0.08922673491945687 1105 12-13 0.09650897161819216 1105 14-15 0.11772099382952916 1105 16-17 0.06495633703744375 1105 18-19 0.102067250845991 1105 20-21 0.10670838670980487 1105 22-23 0.140757151337489 1105 24-25 0.12225100150585888 1105 26-27 0.08235222133935594 1105 28-29 0.08564907639830466 1105 30-31 0.05136851101683959 1105 32-33 0.0789269397612955 1105 34-35 0.0919058876780312 1105 36-37 0.06756114987743445 1105 38-39 0.06926802475106086 1105 40-41 0.05137890227975106 1105 42-43 0.09411976768486596 1105 44-45 0.08014020674639966 1105 46-47 0.07601664298292121 1105 48-49 0.1079089683137866 1105 50-51 0.08036035943970177 1105 52-53 0.15500980079644222 1105 54-55 0.15515455670036005 1105 56-57 0.213730366958778 1105 58-59 0.18773922634660067 1105 60-61 0.1882612355273352 1105 62-63 0.15855224834198367 1105 64-65 0.1584947899111242 1105 66-67 0.15806573692679393 1105 68-69 0.14129401794049556 1105 70-71 0.057424714941035404 1105 72-73 0.08660291796564934 1105 74-75 0.08390430829392415 1105 76-77 0.13569919351353832 1105 78-79 0.13126602043788438 1105 80-81 0.11564539822981601 1105 82-83 0.08126160847888286 1105 84-85 0.0613732070370645 1105 86-87 0.11677706757076933 1105 88-89 0.10336652072629704 1105 90-91 0.08872559063782504 1105 92-93 0.03683929255815244 1105 94-95 0.024385941783449994 1105 96-97 0.11951935680676584 1105 98-99 -0.007792446213962023 1105 100 -0.05588102628602343 1106 1 -0.022025057970232353 1106 2 -0.02353740912275981 1106 3 0.013901417927911552 1106 4 0.013759210445662973 1106 5 0.029663217473213876 1106 6 0.007433731549191691 1106 7 0.0336694147417802 1106 8 0.03618958228608449 1106 9 -0.007616025935156756 1106 10-11 0.04948969950347504 1106 12-13 0.020548472220738745 1106 14-15 -0.007949582900948826 1106 16-17 0.007633586365891176 1106 18-19 -0.03428518687749005 1106 20-21 -0.0018288057201019114 1106 22-23 -0.012178109784976243 1106 24-25 0.017335899659315146 1106 26-27 0.004388449422798146 1106 28-29 -0.011252901072218435 1106 30-31 0.02281218075060565 1106 32-33 0.02346540514847817 1106 34-35 -0.01531781005729016 1106 36-37 -0.07440067068316125 1106 38-39 -0.08928851316405684 1106 40-41 -0.011006965431583637 1106 42-43 0.01511240498506794 1106 44-45 0.013256421816301156 1106 46-47 -0.002571122531243475 1106 48-49 -0.004640275732413102 1106 50-51 -0.1947030170912427 1106 52-53 -0.06394288729757847 1106 54-55 0.018002257876908345 1106 56-57 0.007031881683865038 1106 58-59 0.07275517301837198 1106 60-61 0.12610991887015643 1106 62-63 0.09349431995330093 1106 64-65 0.06655388062733181 1106 66-67 0.13146826890733365 1106 68-69 0.12065958221118933 1106 70-71 0.08543409475073815 1106 72-73 -0.02441902324106593 1106 74-75 -0.07301733105693842 1106 76-77 0.011236493805242276 1106 78-79 -0.0016779150635315432 1106 80-81 0.037948319386089224 1106 82-83 0.07972537876239372 1106 84-85 0.03763793881172717 1106 86-87 0.03534449913301074 1106 88-89 0.04586827418645356 1106 90-91 0.0017491085667167283 1106 92-93 -0.05172002767074346 1106 94-95 -0.029732672774507307 1106 96-97 -0.04564325823769266 1106 98-99 -0.12802978999830827 1106 100 -0.2103715770247092 1107 1 -0.048189413798624514 1107 2 -0.022556829672794265 1107 3 -0.015575980951645363 1107 4 0.01462270947290989 1107 5 -0.008152372300671118 1107 6 -0.005746227523090397 1107 7 -0.004220199014071113 1107 8 -0.024780195867727173 1107 9 -0.003174786474943403 1107 10-11 0.032556158198872254 1107 12-13 0.013054902341210095 1107 14-15 -0.019104199215206563 1107 16-17 -0.029315893498079504 1107 18-19 -0.03541100646781814 1107 20-21 0.030233975755159292 1107 22-23 -0.020733300295852075 1107 24-25 -0.04697504602787461 1107 26-27 -0.027172624474182783 1107 28-29 -0.054568964995489466 1107 30-31 0.004112601475519284 1107 32-33 -0.07947480895434467 1107 34-35 -0.05223140577874119 1107 36-37 -0.08218516820758026 1107 38-39 -0.06410114937809652 1107 40-41 -0.10777586395294492 1107 42-43 -0.06067548069049877 1107 44-45 -0.07737103070354578 1107 46-47 -0.10200322770881343 1107 48-49 -0.16987332735384086 1107 50-51 -0.32683729346235424 1107 52-53 -0.07998789574357801 1107 54-55 0.00955106285902474 1107 56-57 0.04600317069529325 1107 58-59 0.04081051235490918 1107 60-61 0.0779109383897918 1107 62-63 0.05313818498684242 1107 64-65 0.03947455624209795 1107 66-67 0.08896702500216946 1107 68-69 0.04306896369305235 1107 70-71 0.05728487790165104 1107 72-73 0.0750296196240754 1107 74-75 0.08335345262553062 1107 76-77 0.06587803160135763 1107 78-79 0.0766046719894149 1107 80-81 0.0426565326887669 1107 82-83 0.006095797380606172 1107 84-85 -0.002579009902209606 1107 86-87 0.012841658181841353 1107 88-89 0.033753946607440355 1107 90-91 0.06456231743205976 1107 92-93 -0.08122862878466464 1107 94-95 -0.08608158972475621 1107 96-97 0.03230371100100626 1107 98-99 -0.08579117685388127 1107 100 -0.08367945858830339 1108 1 -0.0461367941989792 1108 2 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-1.610347694747503 2316 100 -1.2213013716728724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 679.0 3 1699.0 4 2796.0 5 9096.0 6 8471.0 7 9315.0 8 21214.0 9 30161.0 10 32998.0 11 31664.0 12 32176.0 13 36430.0 14 44088.0 15 56845.0 16 78348.0 17 104704.0 18 124242.0 19 149153.0 20 182850.0 21 227760.0 22 289127.0 23 376049.0 24 453457.0 25 492175.0 26 497010.0 27 485077.0 28 504909.0 29 578025.0 30 690261.0 31 850449.0 32 1071859.0 33 1399490.0 34 1954728.0 35 3008393.0 36 5103409.0 37 9114199.0 38 9649866.0 39 2412308.0 40 4352.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.333260891192 27.249478162136885 26.99545420574027 26.421806740930837 2 21.940401287614588 31.654600006786787 25.996693423446178 20.408305282152448 3 23.652004089238257 24.778197517108588 28.060505289079558 23.509293104573597 4 19.056161603228603 28.006604566806708 24.76867861996234 28.16855521000235 5 23.049747242360656 28.547892729093487 26.355481829937986 22.046878198607867 6 26.762989281616267 28.79099413464077 24.670036462497123 19.775980121245844 7 22.272091321618355 25.632411021868958 28.562226358817806 23.53327129769488 8 25.099336951012614 27.279503999863202 24.67173781889869 22.949421230225493 9 23.467319581112204 24.5093345848042 27.940644966129923 24.082700867953672 10-11 22.30972553179201 27.808435654323453 26.522940157397528 23.358898656487 12-13 22.602131222881088 26.936624040995945 26.32686069339611 24.134384042726857 14-15 22.6303840076198 28.68263311252524 25.91744721676925 22.76953566308571 16-17 23.720312816025064 26.360466151541402 26.03342112725197 23.88579990518157 18-19 22.502560130541397 27.345025812367897 26.65538538525028 23.497028671840425 20-21 23.605965104853013 27.403310051960727 26.814001282564693 22.176723560621568 22-23 23.472644772143937 26.094390833114467 27.949331912719693 22.483632482021903 24-25 22.57176048679466 26.793742979688602 27.02249661306977 23.611999920446973 26-27 22.68967835073881 25.81323553372593 29.225953442802428 22.27113267273283 28-29 22.684334256844036 25.927688592909444 29.136494868656737 22.251482281589784 30-31 22.458592539340284 25.84812586355657 29.278456374659314 22.41482522244384 32-33 22.556916048078307 26.177921207059267 28.954978507873854 22.310184236988576 34-35 22.539987234268835 26.393350061686395 28.82708487998483 22.239577824059936 36-37 22.469960091354725 26.383926684846998 28.95840543190476 22.18770779189352 38-39 22.529878745954093 26.475396450641636 28.928856267266646 22.06586853613762 40-41 22.59578085967659 26.625699322719402 28.783572186714036 21.99494763088997 42-43 22.696109882208727 26.684496623713667 28.67652215029087 21.94287134378673 44-45 22.87816513328461 26.77147393243852 28.527186387511794 21.82317454676507 46-47 22.980938652887716 26.9316827559834 28.312782603457205 21.77459598767168 48-49 23.159588033653097 27.04564388442013 28.098703606129337 21.69606447579743 50-51 23.44094585825904 27.129125387908182 27.852606135404628 21.577322618428145 52-53 23.685611704594354 27.203429331230637 27.585039544377516 21.525919419797493 54-55 23.896274045521118 27.376859749769935 27.22057636941394 21.506289835295007 56-57 24.298831203079597 27.590994077058735 26.728455688381274 21.381719031480397 58-59 24.553177642960698 27.79635779303203 26.41971876446554 21.230745799541733 60-61 24.825514416669904 27.891957732941513 26.256883299975765 21.025644550412817 62-63 25.05957641017672 27.98929964958252 26.13231357321683 20.81881036702392 64-65 25.322690343031585 28.02322248963583 26.04801421476106 20.606072952571523 66-67 25.54501317249347 28.013617125702282 26.067367166291522 20.374002535512727 68-69 25.8092644807107 28.047276902128736 25.985020810708647 20.15843780645192 70-71 26.067829761546633 28.01931463708847 25.93052108716782 19.98233451419708 72-73 26.336989415978522 27.997713208688 25.851094926870573 19.814202448462908 74-75 26.67603830963249 27.971603784539735 25.706174970100825 19.646182935726955 76-77 26.999612631811814 27.9267006844869 25.51029163725716 19.563395046444125 78-79 27.287280278523944 27.867114249571284 25.396751639564634 19.44885383234013 80-81 27.56842532162787 27.79686252703131 25.249825119141267 19.38488703219955 82-83 27.788287683154973 27.702228250758733 25.12032176828436 19.38916229780194 84-85 27.936844859268785 27.577331816450148 25.097135482509024 19.38868784177204 86-87 28.094452159688878 27.514368340454855 25.049825930685127 19.341353569171144 88-89 28.13987043166976 27.422758258192424 25.06304993778667 19.374321372351147 90-91 28.196197739791874 27.40628099850056 25.047104774752523 19.35041648695504 92-93 28.216008226708528 27.49381898104073 25.034053646186916 19.25611914606382 94-95 28.171523261924257 27.633908366113403 24.97490636251637 19.21966200944597 96-97 28.097646592739455 27.837510390733 24.90509770153404 19.159745314993508 98-99 28.060057055280787 28.11418379384221 24.78854242861887 19.037216722258137 100 27.937051849789945 28.37081742278822 24.695728608948937 18.9964021184729 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 29011.0 1 22682.0 2 13673.0 3 10382.0 4 10608.0 5 11415.5 6 12742.5 7 20612.5 8 25742.5 9 24955.0 10 25403.5 11 30619.5 12 80019.5 13 90491.5 14 57356.0 15 61170.5 16 68397.5 17 75191.5 18 81421.0 19 88834.0 20 98661.5 21 113173.0 22 133732.5 23 158246.0 24 182607.5 25 208197.5 26 236714.5 27 269874.5 28 317922.5 29 372540.0 30 417627.5 31 465042.5 32 519285.0 33 570080.0 34 621962.0 35 688958.0 36 761970.5 37 817008.0 38 875035.0 39 941294.5 40 990372.0 41 1043898.0 42 1113512.0 43 1167321.5 44 1212244.5 45 1274915.0 46 1355851.0 47 1451437.5 48 1547684.5 49 1623633.0 50 1668355.5 51 1688903.0 52 1682625.5 53 1649131.0 54 1591777.5 55 1504350.0 56 1390199.0 57 1253628.5 58 1097063.5 59 925154.0 60 749856.5 61 591611.0 62 459722.5 63 351941.0 64 267305.0 65 201638.0 66 151501.5 67 114828.0 68 87665.5 69 68451.0 70 54452.5 71 43383.0 72 35001.0 73 27498.5 74 21765.5 75 17995.0 76 13676.0 77 9008.0 78 6051.5 79 4396.5 80 3130.5 81 2291.5 82 1686.0 83 1268.0 84 1007.0 85 793.0 86 656.5 87 556.0 88 475.0 89 408.0 90 338.5 91 285.5 92 229.5 93 176.5 94 140.5 95 116.0 96 102.5 97 83.5 98 55.5 99 41.0 100 24.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08142606379807373 2 0.03112924301377932 3 0.021670080772023172 4 0.01589238957929834 5 0.01177472527801213 6 0.011570337582669838 7 0.01310075276486701 8 0.006443197469022303 9 0.001595221036817901 10-11 0.0022557422473753128 12-13 0.00573531813393436 14-15 0.05268092847447617 16-17 0.05518966280815932 18-19 0.09201185089708251 20-21 0.11131776424188415 22-23 0.11015125885871108 24-25 0.11448951231899475 26-27 0.11274598557641019 28-29 0.10911311891834442 30-31 0.09324939346705141 32-33 0.09080671125442401 34-35 0.11304882832011859 36-37 0.11449075858542977 38-39 0.10158816218372999 40-41 0.08495299780916331 42-43 0.08455917761569888 44-45 0.0826798078316978 46-47 0.0788562624090749 48-49 0.08374038056789469 50-51 0.08673640507766832 52-53 0.09462776414417688 54-55 0.10467142534395459 56-57 0.10979731919116709 58-59 0.10424769475604982 60-61 0.09277830475461613 62-63 0.10022474670382468 64-65 0.09921651715789837 66-67 0.09569456821254885 68-69 0.09876786124129333 70-71 0.10530328242650668 72-73 0.12391128656770048 74-75 0.1344210514141734 76-77 0.1296341420372847 78-79 0.12075698622068008 80-81 0.118521184236265 82-83 0.10542417027070301 84-85 0.11025594523925225 86-87 0.11608099455650761 88-89 0.11772481998429106 90-91 0.1242515173044593 92-93 0.12911943399962394 94-95 0.14427777264869904 96-97 0.1467977233802973 98-99 0.1384103502726532 100 0.1402199291362935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.0119832E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.87784964303816 #Duplication Level Percentage of deduplicated Percentage of total 1 75.84659541690118 34.796787004729396 2 11.723332927374337 10.756826107147162 3 4.169031781132862 5.73798639635583 4 2.3154490205323244 4.249112880804078 5 1.335677848782288 3.0639013758985234 6 0.8722019622801511 2.400885029031097 7 0.6416488663817119 2.0606229150840245 8 0.46513186448034516 1.707139979425222 9 0.3597774867637503 1.4855235698427738 >10 1.9998535059472917 16.461857185381746 >50 0.16725082637064542 5.385226508598435 >100 0.1020434496811594 8.469015574742023 >500 0.001387209174487902 0.4119347685294153 >1k 5.474246499711954E-4 0.40654710094433094 >5k 1.624835729137826E-5 0.054245646374893874 >10k+ 5.41611902137383E-5 2.5523879571111143 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 725948 1.809449251931065 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78574 0.19584827772957775 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 53908 0.13436746195746782 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.018280236068785132 1.4955197220167821E-5 0.0 2.49253287002797E-6 4.98506574005594E-6 2 0.018287713667395216 2.2432795830251733E-5 0.0 2.49253287002797E-6 1.4955197220167821E-5 3 0.018290206200265245 2.2432795830251733E-5 0.0 4.98506574005594E-6 2.2432795830251733E-5 4 0.018292698733135274 2.7417861570307674E-5 0.0 7.4775986100839105E-6 3.738799305041955E-5 5 0.0182951912660053 3.4895460180391586E-5 0.0 7.4775986100839105E-6 4.735812453053143E-5 6 0.01829768379887533 3.738799305041955E-5 0.0 7.4775986100839105E-6 5.483572314061535E-5 7 0.01829768379887533 4.4865591660503466E-5 0.0 7.4775986100839105E-6 6.480585462072723E-5 8 0.018300176331745358 4.735812453053143E-5 0.0 7.4775986100839105E-6 7.726851897086707E-5 9 0.018300176331745358 5.732825601064331E-5 0.0 7.4775986100839105E-6 9.471624906106286E-5 10-11 0.018308900196790455 6.231332175069926E-5 0.0 8.723865045097895E-6 1.0593264697618873E-4 12-13 0.018322609127575606 7.976105184089505E-5 0.0 1.1216397915125867E-5 1.1964157776134257E-4 14-15 0.018340056857665807 9.222371619103489E-5 0.0 1.246266435013985E-5 1.3958184072156632E-4 16-17 0.018364982186366084 9.596251549607684E-5 0.0 1.4955197220167821E-5 1.5329077150672015E-4 18-19 0.018399877646546478 1.0468638054117475E-4 0.0 1.4955197220167821E-5 1.66999702291874E-4 20-21 0.018478392431952356 1.2088784419635655E-4 0.0 1.7447730090195793E-5 1.7572356733697191E-4 22-23 0.019100279383024336 1.3085797567646845E-4 0.0 1.994026296022376E-5 1.919250309921537E-4 24-25 0.02201654284095706 1.4082810715658034E-4 0.0 2.6171595135293688E-5 2.1435782682240544E-4 26-27 0.034167640582343416 1.4830570576666424E-4 0.0 2.8664128005321656E-5 2.268204911725453E-4 28-29 0.07168275280913439 1.520445050717062E-4 0.0 3.2402927310363614E-5 2.49253287002797E-4 30-31 0.15884288847470746 1.66999702291874E-4 1.246266435013985E-6 3.4895460180391586E-5 2.667010170929928E-4 32-33 0.3234161100176093 1.6824596872688799E-4 2.49253287002797E-6 3.988052592044752E-5 2.891338129232446E-4 34-35 0.5837673996242059 1.7946236664201387E-4 2.49253287002797E-6 3.988052592044752E-5 3.015964772733844E-4 36-37 0.9468471852025702 1.8569369881708377E-4 2.49253287002797E-6 3.988052592044752E-5 3.1530540805853823E-4 38-39 1.4111848723593856 1.9067876455713972E-4 2.49253287002797E-6 3.988052592044752E-5 3.2153674023360817E-4 40-41 1.9764202900949335 1.9691009673220966E-4 2.49253287002797E-6 4.112679235546151E-5 3.3026060527870603E-4 42-43 2.6414367836834414 2.081264946473355E-4 2.49253287002797E-6 4.2373058790475495E-5 3.4521580249887384E-4 44-45 3.392734795100837 2.168503596924334E-4 2.49253287002797E-6 4.2373058790475495E-5 3.6141726615405566E-4 46-47 4.226085742333119 2.3180555691260123E-4 2.49253287002797E-6 4.2373058790475495E-5 3.701411311991536E-4 48-49 5.140323119997113 2.4052942195769913E-4 2.49253287002797E-6 4.4865591660503466E-5 3.863425948543354E-4 50-51 6.12568367684092 2.50499553437811E-4 2.49253287002797E-6 4.4865591660503466E-5 3.925739270294053E-4 52-53 7.1758426107068445 2.604696849179229E-4 2.49253287002797E-6 4.6111858095517446E-5 4.087753906845871E-4 54-55 8.279058845510619 2.691935499630208E-4 2.49253287002797E-6 4.98506574005594E-5 4.112679235546151E-4 56-57 9.42208332278161 2.816562143131606E-4 2.49253287002797E-6 4.98506574005594E-5 4.1749925572968504E-4 58-59 10.60147883969205 2.916263457932725E-4 2.49253287002797E-6 4.98506574005594E-5 4.2746938720979687E-4 60-61 11.812303949827108 2.9785767796834244E-4 2.49253287002797E-6 4.98506574005594E-5 4.3370071938486686E-4 62-63 13.051922799676728 2.991039444033564E-4 2.49253287002797E-6 5.483572314061535E-5 4.424245844299647E-4 64-65 14.307657120797515 3.0533527657842634E-4 2.49253287002797E-6 5.483572314061535E-5 4.573797816501326E-4 66-67 15.57475739180563 3.140591416235242E-4 2.49253287002797E-6 5.85745224456573E-5 4.7732004461035625E-4 68-69 16.846270692260127 3.165516744935522E-4 2.49253287002797E-6 6.106705531568526E-5 4.9726030757058E-4 70-71 18.124963733646744 3.1779794092856616E-4 2.49253287002797E-6 6.480585462072723E-5 5.047379061806639E-4 72-73 19.40428738584947 3.277680724086781E-4 2.49253287002797E-6 6.72983874907552E-5 5.334020341859856E-4 74-75 20.685801974444956 3.35245671018762E-4 2.49253287002797E-6 6.72983874907552E-5 5.483572314061534E-4 76-77 21.962444907545972 3.4521580249887384E-4 2.49253287002797E-6 6.979092036078317E-5 5.583273628862653E-4 78-79 23.23086995977451 3.502008682389298E-4 2.49253287002797E-6 6.979092036078317E-5 5.658049614963492E-4 80-81 24.494080633238944 3.576784668490137E-4 2.49253287002797E-6 7.103718679579715E-5 5.745288265414471E-4 82-83 25.74747072719547 3.6640233189411163E-4 2.49253287002797E-6 7.228345323081113E-5 5.820064251515311E-4 84-85 26.98962448297391 3.788649962442515E-4 2.49253287002797E-6 7.228345323081113E-5 5.957153559366849E-4 86-87 28.2220062636354 3.913276605943913E-4 2.49253287002797E-6 7.352971966582511E-5 6.156556188969087E-4 88 29.13755969865477 4.012977920745032E-4 2.49253287002797E-6 8.225358471092302E-5 6.181481517669366E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 123180 0.0 70.250565 1 GCAGTGG 138125 0.0 62.44049 3 AGCAGTG 142025 0.0 61.000896 2 GTGGTAT 154520 0.0 55.193638 6 AGTGGTA 155600 0.0 54.832554 5 CAGTGGT 163130 0.0 52.538437 4 TGGTATC 165960 0.0 51.27592 7 GGTATCA 168080 0.0 50.700283 8 GTATCAA 190175 0.0 44.771076 9 AGTACTT 170020 0.0 24.696201 20-21 GAGTACT 159405 0.0 23.837307 20-21 TACTTTT 182535 0.0 23.116264 22-23 GTACTTT 166165 0.0 22.89865 22-23 ATCAACG 212735 0.0 22.570667 10-11 CAACGCA 217180 0.0 22.061314 12-13 ACGCAGA 224605 0.0 21.303616 14-15 AGAGTAC 222220 0.0 21.031708 18-19 TCAACGC 213410 0.0 20.019255 12-13 TATCAAC 213800 0.0 19.945534 10-11 ACTTTTT 197475 0.0 19.520784 24-25 >>END_MODULE