FastQCFastQC Report
Thu 26 May 2016
SRR1660645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40119832
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7436161.853487322678719No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT832040.20738870491780723No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT558800.13928273677716296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1235650.070.569981
GCAGTGG1394300.062.3022353
AGCAGTG1441350.060.653212
GTGGTAT1564100.055.2111786
AGTGGTA1578950.054.7544175
CAGTGGT1641750.052.8402824
TGGTATC1691500.050.9638987
GGTATCA1720950.050.2583358
GTATCAA1953300.044.265549
AGTACTT1754000.024.42614720-21
GAGTACT1643300.023.66908520-21
TACTTTT1878900.022.87597722-23
GTACTTT1717700.022.617422-23
ATCAACG2168850.022.39225810-11
CAACGCA2215950.021.88450212-13
ACGCAGA2295350.021.13157514-15
AGAGTAC2290900.020.86773118-19
TCAACGC2176050.019.86500512-13
TATCAAC2187050.019.81021110-11
GCAGAGT2521350.019.294316-17
Produced by FastQC (version 0.11.5)