##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660645_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40119832 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52676402034784 34.0 31.0 34.0 31.0 34.0 2 32.76944537055888 34.0 31.0 34.0 31.0 34.0 3 32.84044900786225 34.0 31.0 34.0 31.0 34.0 4 36.24412719375295 37.0 37.0 37.0 35.0 37.0 5 36.14244080084882 37.0 35.0 37.0 35.0 37.0 6 36.16366392062659 37.0 37.0 37.0 35.0 37.0 7 36.06832792819272 37.0 36.0 37.0 35.0 37.0 8 36.114177646606294 37.0 36.0 37.0 35.0 37.0 9 37.768202992475146 39.0 38.0 39.0 35.0 39.0 10-11 37.79185471165482 39.0 38.0 39.0 35.0 39.0 12-13 37.81658042586021 39.0 38.0 39.0 35.0 39.0 14-15 39.21498798898261 41.0 39.0 41.0 36.0 41.0 16-17 39.16359981268117 40.5 39.0 41.0 36.0 41.0 18-19 39.15860287002199 40.0 39.0 41.0 36.0 41.0 20-21 39.1018128141713 40.0 39.0 41.0 36.0 41.0 22-23 39.01746834333703 40.0 38.5 41.0 35.5 41.0 24-25 38.995019670072395 40.0 39.0 41.0 35.0 41.0 26-27 38.86111903459616 40.0 38.0 41.0 35.0 41.0 28-29 38.760675032238424 40.0 38.0 41.0 35.0 41.0 30-31 38.61576357049551 40.0 38.0 41.0 34.5 41.0 32-33 38.456723335730814 40.0 38.0 41.0 34.0 41.0 34-35 38.3922647906402 40.0 38.0 41.0 34.0 41.0 36-37 38.3902191564511 40.0 38.0 41.0 34.0 41.0 38-39 38.188394545121724 40.0 38.0 41.0 34.0 41.0 40-41 38.06551185209349 40.0 37.0 41.0 33.0 41.0 42-43 37.918050292433925 40.0 37.0 41.0 33.0 41.0 44-45 37.75082298450303 40.0 37.0 41.0 33.0 41.0 46-47 37.55397252161973 40.0 36.5 41.0 33.0 41.0 48-49 37.341371668754746 40.0 36.0 41.0 32.0 41.0 50-51 37.37138880591524 40.0 36.0 41.0 32.0 41.0 52-53 37.187375348431175 40.0 36.0 41.0 31.5 41.0 54-55 36.863461828554016 39.0 35.5 41.0 31.0 41.0 56-57 36.53363651921573 39.0 35.0 41.0 30.0 41.0 58-59 36.25004508991962 39.0 35.0 41.0 30.0 41.0 60-61 36.0248411434026 38.5 35.0 40.5 29.0 41.0 62-63 35.772790474297096 38.0 35.0 40.0 29.0 41.0 64-65 35.44093690372382 37.0 35.0 40.0 29.0 41.0 66-67 35.12359135751117 37.0 34.0 39.5 28.0 41.0 68-69 34.80679097808785 36.0 34.0 39.0 28.0 41.0 70-71 34.46522425617336 35.5 34.0 39.0 28.0 40.5 72-73 34.07932232866777 35.0 34.0 38.0 27.5 40.0 74-75 33.71213284243065 35.0 33.5 37.0 27.0 39.5 76-77 31.95617360760633 34.0 31.0 35.5 25.0 38.5 78-79 33.09420121699413 35.0 33.0 36.0 26.5 39.0 80-81 33.06629164100188 35.0 33.0 36.0 27.0 37.5 82-83 32.864555813693336 35.0 33.0 35.5 26.5 37.0 84-85 32.65323198511898 35.0 33.0 35.0 26.0 37.0 86-87 32.45231355903982 35.0 33.0 35.0 26.0 36.0 88-89 32.27925389867036 35.0 33.0 35.0 25.5 36.0 90-91 32.14616933091843 35.0 33.0 35.0 25.0 36.0 92-93 32.00396631770542 35.0 33.0 35.0 25.0 35.0 94-95 31.88484237670786 35.0 33.0 35.0 25.0 35.0 96-97 31.769398884820852 35.0 33.0 35.0 24.0 35.0 98-99 31.66193158535659 35.0 32.0 35.0 24.0 35.0 100 31.567088167268498 35.0 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21190818970563186 1101 2 0.20900374516727993 1101 3 0.21401839272743217 1101 4 0.15878907619796934 1101 5 0.14221703305159394 1101 6 0.14921351923781145 1101 7 0.16130681326957585 1101 8 0.17258933540130528 1101 9 0.31005375323382367 1101 10-11 0.24688717060801935 1101 12-13 0.20258122493857655 1101 14-15 0.2616930431770683 1101 16-17 0.32067848894899953 1101 18-19 0.2949566213526751 1101 20-21 0.2772947196344404 1101 22-23 0.2642854269152153 1101 24-25 0.23641675519898797 1101 26-27 0.2418516106530646 1101 28-29 0.21395753840233311 1101 30-31 0.2803772408672103 1101 32-33 0.26750370772804644 1101 34-35 0.2498973613650719 1101 36-37 0.25124192900576503 1101 38-39 0.24617305192703753 1101 40-41 0.25223332280937427 1101 42-43 0.22231819586763635 1101 44-45 0.22315696373188842 1101 46-47 0.15850355020270968 1101 48-49 0.16995650791194805 1101 50-51 0.24973412901083236 1101 52-53 0.2648956329611849 1101 54-55 0.25750954862676423 1101 56-57 0.21176577907692007 1101 58-59 0.2528052330269617 1101 60-61 0.1813396432562442 1101 62-63 0.0835379886093719 1101 64-65 0.1592401471414675 1101 66-67 0.1414385833981342 1101 68-69 0.12893276684221178 1101 70-71 0.15167494357742584 1101 72-73 0.11559696258627383 1101 74-75 0.09487620358342497 1101 76-77 0.1253127694055607 1101 78-79 0.19904498918965174 1101 80-81 0.21953320278709754 1101 82-83 0.16022679122539785 1101 84-85 0.06699858385347568 1101 86-87 0.16674212864722904 1101 88-89 0.15967222836003714 1101 90-91 0.12437467108230038 1101 92-93 0.11425794961017743 1101 94-95 0.09435796501015048 1101 96-97 0.0767094167482476 1101 98-99 0.0861306397390571 1101 100 0.08971843502644461 1102 1 0.12700719754809597 1102 2 0.15044433441987337 1102 3 0.1743718059641779 1102 4 0.1351053948690364 1102 5 0.09370790767491854 1102 6 0.14106003540201328 1102 7 0.22184974224038 1102 8 0.15818891386741285 1102 9 0.2823033359454641 1102 10-11 0.19410358125315952 1102 12-13 0.16671366843974766 1102 14-15 0.24272934221969678 1102 16-17 0.2765741344049033 1102 18-19 0.23448660253068 1102 20-21 0.20793656309597708 1102 22-23 0.17697633934464818 1102 24-25 0.1795548266625886 1102 26-27 0.20306973378813353 1102 28-29 0.22431801981384325 1102 30-31 0.18613983701400372 1102 32-33 0.22807975070879394 1102 34-35 0.21700183507927306 1102 36-37 0.21198578217013875 1102 38-39 0.21494605765127517 1102 40-41 0.13988473318578798 1102 42-43 0.15667586561289681 1102 44-45 0.17358376341594806 1102 46-47 0.21116728329000267 1102 48-49 0.17020682679543597 1102 50-51 0.2108035755460591 1102 52-53 0.20551379732118136 1102 54-55 0.16955162894324616 1102 56-57 0.1275545398098643 1102 58-59 0.1933260614037451 1102 60-61 0.21025410889403418 1102 62-63 0.16076946700128758 1102 64-65 0.2015017627504534 1102 66-67 0.1439640271159064 1102 68-69 0.12326000941122572 1102 70-71 0.1436724165283536 1102 72-73 0.1228639918104264 1102 74-75 0.046007666783857815 1102 76-77 0.011729222313611842 1102 78-79 0.1430939617326672 1102 80-81 0.2081765293313751 1102 82-83 0.18467252185276095 1102 84-85 0.17644419012245294 1102 86-87 0.175425311304636 1102 88-89 0.16457989570398013 1102 90-91 0.10682042945381909 1102 92-93 0.06730536492149497 1102 94-95 0.06935505154141097 1102 96-97 0.12102509436799735 1102 98-99 0.07222770756647634 1102 100 0.10287441018083854 1103 1 0.1282324639107557 1103 2 0.12191792807073654 1103 3 0.14567511169062186 1103 4 0.0896661159570371 1103 5 0.07369195247285631 1103 6 0.09333514071328608 1103 7 0.1628658609745841 1103 8 0.11481423434038618 1103 9 0.2309942147090709 1103 10-11 0.15737866719494775 1103 12-13 0.12681349116179774 1103 14-15 0.16837845479046365 1103 16-17 0.23788670594468897 1103 18-19 0.19871062173445608 1103 20-21 0.132124246481375 1103 22-23 0.17064482222070154 1103 24-25 0.1619451112749246 1103 26-27 0.2051024232807208 1103 28-29 0.13303183035687738 1103 30-31 0.15060987203245446 1103 32-33 0.1541701094519894 1103 34-35 0.21582099072634264 1103 36-37 0.1713446785058821 1103 38-39 0.15569610993571104 1103 40-41 0.14266473112069633 1103 42-43 0.10112913865862083 1103 44-45 0.12830683420828137 1103 46-47 0.10435841543658597 1103 48-49 0.15350044022344633 1103 50-51 0.14009433184597953 1103 52-53 0.14390060357264645 1103 54-55 0.20199429041704065 1103 56-57 0.18525635278620456 1103 58-59 0.1963325944138674 1103 60-61 0.1698676315088079 1103 62-63 0.16273906477989186 1103 64-65 0.20524197826906487 1103 66-67 0.16002506616963075 1103 68-69 0.1275561174384663 1103 70-71 0.13024790126971908 1103 72-73 0.08855536401295439 1103 74-75 0.044442378572981056 1103 76-77 -0.019481302259514166 1103 78-79 0.16041985706389283 1103 80-81 0.18595185433510153 1103 82-83 0.17316691157232356 1103 84-85 0.1711783825332489 1103 86-87 0.14963962855043178 1103 88-89 0.18172223345866456 1103 90-91 0.13777780482443802 1103 92-93 0.12344183696191635 1103 94-95 0.1059852179263423 1103 96-97 0.18463357733419983 1103 98-99 0.10842180181126437 1103 100 0.16346975399947183 1104 1 0.07587403325111808 1104 2 0.0979926736021639 1104 3 0.09613639832284093 1104 4 0.09415374174094637 1104 5 0.04513526559952652 1104 6 0.0788438042063504 1104 7 0.07975184187215234 1104 8 0.0856341863108554 1104 9 0.24031240374324625 1104 10-11 0.12857428883269506 1104 12-13 0.09546876466065868 1104 14-15 0.1290652399495329 1104 16-17 0.15005849147529204 1104 18-19 0.09863165250757078 1104 20-21 0.140363423525379 1104 22-23 0.12736456177108835 1104 24-25 0.09113165171910964 1104 26-27 0.09042696775404835 1104 28-29 0.12042410967587358 1104 30-31 0.10657564110088913 1104 32-33 0.13885159526721935 1104 34-35 0.12656915408675928 1104 36-37 0.1270831663087506 1104 38-39 0.1063215510749771 1104 40-41 0.09109140315694475 1104 42-43 0.07068518923582445 1104 44-45 0.08345831380558622 1104 46-47 0.08645364439596648 1104 48-49 0.12500027496764687 1104 50-51 0.11614781001816965 1104 52-53 0.07439342948043759 1104 54-55 0.052057438993571736 1104 56-57 0.04082262536846315 1104 58-59 0.08238426802933674 1104 60-61 0.08545727491368638 1104 62-63 0.09104024620195617 1104 64-65 0.1388332230946645 1104 66-67 0.1512791010458585 1104 68-69 0.0639699392728943 1104 70-71 0.04389477608374648 1104 72-73 -0.050231240572003344 1104 74-75 -0.039065650307072985 1104 76-77 -0.10691520825242762 1104 78-79 0.04127598079167427 1104 80-81 0.14304779341856744 1104 82-83 0.15922844588668283 1104 84-85 0.16181310297355367 1104 86-87 0.12216383676882714 1104 88-89 0.07381485527045584 1104 90-91 0.06857408280592381 1104 92-93 0.0898822064242566 1104 94-95 0.12618885335855268 1104 96-97 0.13964401911927737 1104 98-99 0.1025220550621917 1104 100 0.12494961581545638 1105 1 0.054814551648334486 1105 2 0.09180599046466398 1105 3 0.08253922424111693 1105 4 0.08606226187662003 1105 5 0.04783363381976358 1105 6 0.06630458661122418 1105 7 0.1335388557711994 1105 8 0.10210783894928 1105 9 0.22256555826169944 1105 10-11 0.09711373544291035 1105 12-13 0.07225001887082527 1105 14-15 0.09397126158736313 1105 16-17 0.1587882507023579 1105 18-19 0.08265247500403206 1105 20-21 0.0697715440328821 1105 22-23 0.12601147706486415 1105 24-25 0.08935806811842184 1105 26-27 0.10224437806982678 1105 28-29 0.09064588957787834 1105 30-31 0.045117764431857665 1105 32-33 0.070594450500856 1105 34-35 0.08811639662084758 1105 36-37 0.1166376344804263 1105 38-39 0.08743591164468256 1105 40-41 0.08130006827546765 1105 42-43 0.098960559748555 1105 44-45 0.09511762333635687 1105 46-47 0.04401610999417471 1105 48-49 0.06609326432326412 1105 50-51 0.0892288675797559 1105 52-53 0.08933389456387886 1105 54-55 0.11572940770918905 1105 56-57 0.13207657031766473 1105 58-59 0.11235772618954343 1105 60-61 0.08609519404821242 1105 62-63 0.03226618791054392 1105 64-65 0.06737398161099861 1105 66-67 0.033814940150648454 1105 68-69 0.04002582895012097 1105 70-71 0.07078320043834196 1105 72-73 0.029528137339092098 1105 74-75 0.0013334989869875358 1105 76-77 -0.08205833592667489 1105 78-79 0.049644746322009325 1105 80-81 0.09289066412402036 1105 82-83 0.11857437884712851 1105 84-85 0.11576329574968014 1105 86-87 0.17547003606220812 1105 88-89 0.171205225893047 1105 90-91 0.1256052444374447 1105 92-93 0.07915197048018285 1105 94-95 0.09071108560812391 1105 96-97 0.10648445499721504 1105 98-99 0.15744342197155348 1105 100 0.23455889923868867 1106 1 0.0060745784556459625 1106 2 0.033833129577359955 1106 3 0.0246297835750795 1106 4 0.025147183982490162 1106 5 -0.015384900024074 1106 6 0.010334902004466073 1106 7 0.05483424264195236 1106 8 0.023661369644052854 1106 9 0.1596908192407298 1106 10-11 0.04907913605181591 1106 12-13 0.02512782443238848 1106 14-15 0.04445836443827034 1106 16-17 0.04007851745453195 1106 18-19 0.038025230298238455 1106 20-21 0.010086222284776625 1106 22-23 0.001219006725072802 1106 24-25 -0.025254117694800016 1106 26-27 0.023781114966929806 1106 28-29 0.005732371585153828 1106 30-31 0.006759478125736962 1106 32-33 0.056508322782072185 1106 34-35 0.035362312839190224 1106 36-37 0.012435261859785385 1106 38-39 0.022372490331171946 1106 40-41 0.04169140512706093 1106 42-43 0.05333530604502812 1106 44-45 -0.008881826088419587 1106 46-47 -0.012360948500308666 1106 48-49 0.050517888628384355 1106 50-51 0.04304268951214141 1106 52-53 -0.025087434838837908 1106 54-55 0.004098024909886533 1106 56-57 -0.015513149847890872 1106 58-59 0.019597217906976994 1106 60-61 -0.0016909115105221417 1106 62-63 -0.040414893831055565 1106 64-65 0.029695461653652444 1106 66-67 0.01211871888652638 1106 68-69 5.772931356418098E-4 1106 70-71 0.00572152607139742 1106 72-73 -0.035171772565085746 1106 74-75 -0.07055237607177389 1106 76-77 -0.0914651203974195 1106 78-79 0.0567707858144999 1106 80-81 0.08713806330352725 1106 82-83 0.06680316297156708 1106 84-85 0.017479785576782092 1106 86-87 0.010815932988606392 1106 88-89 0.03251035293335036 1106 90-91 -0.008575070355327341 1106 92-93 0.04187862391056285 1106 94-95 0.03894194102644022 1106 96-97 0.07719149503818556 1106 98-99 0.036609026728253724 1106 100 0.0537146457953952 1107 1 -0.038421061115478494 1107 2 -0.009737462215454684 1107 3 0.013598684442108322 1107 4 0.023922414801951675 1107 5 -0.06715839123244649 1107 6 -0.024299844287774874 1107 7 0.05078762241399204 1107 8 -0.004582817393533389 1107 9 0.08277978935092989 1107 10-11 0.023830052371280885 1107 12-13 -0.025865689567517336 1107 14-15 -0.024164159978404598 1107 16-17 -0.021430957422623464 1107 18-19 -0.036309213899237136 1107 20-21 -0.0548925726541043 1107 22-23 -0.03927827006044282 1107 24-25 -0.02406173428164493 1107 26-27 -0.009816300176801462 1107 28-29 -0.014363074155845368 1107 30-31 0.0025785448314792347 1107 32-33 -0.07115192598536879 1107 34-35 -0.08962405734026646 1107 36-37 -0.023880143802678333 1107 38-39 -0.12781554104039827 1107 40-41 -0.057610563012232774 1107 42-43 -0.09003557320097144 1107 44-45 -0.04715349638625099 1107 46-47 -0.0691918521974273 1107 48-49 -0.07000303597757096 1107 50-51 -0.10771937771446716 1107 52-53 -0.1395483485064517 1107 54-55 -0.10015248200530635 1107 56-57 -0.04799507127079039 1107 58-59 -0.0604908626217977 1107 60-61 -0.03613481514700112 1107 62-63 -0.12728033592884458 1107 64-65 -0.03673197330636668 1107 66-67 -0.10554103664777159 1107 68-69 -0.0481236091670425 1107 70-71 -0.08308948324719978 1107 72-73 -0.12105734652821099 1107 74-75 -0.08965613942618234 1107 76-77 -0.09753788184348267 1107 78-79 0.007798379084796636 1107 80-81 0.05972168746236406 1107 82-83 0.039225916614874734 1107 84-85 0.02359066924456954 1107 86-87 0.05485760616177515 1107 88-89 0.0366972331507327 1107 90-91 -0.01049484366615161 1107 92-93 0.018063607882780275 1107 94-95 0.052561890613848306 1107 96-97 0.027402985657698054 1107 98-99 0.022806042594126552 1107 100 -0.0039042760247660624 1108 1 -0.04537084182163653 1108 2 -0.02736081664512824 1108 3 -0.035965568520012425 1108 4 -0.0146917365205681 1108 5 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-1.825057673052065 2316 50-51 -1.5135855160167253 2316 52-53 -1.0471016448391381 2316 54-55 -1.0009413204503161 2316 56-57 -1.1524942378788836 2316 58-59 -0.9404804032086886 2316 60-61 -0.850126061847007 2316 62-63 -0.8160483084193189 2316 64-65 -0.831899048366445 2316 66-67 -0.7375943396858204 2316 68-69 -0.7064490181753413 2316 70-71 -0.8245281374416678 2316 72-73 -0.7959498130441744 2316 74-75 -0.7648387794675173 2316 76-77 -0.9043437192000106 2316 78-79 -0.8999912744229093 2316 80-81 -0.8200001778044665 2316 82-83 -0.7788368601020963 2316 84-85 -0.8129960423822702 2316 86-87 -0.7744842752043084 2316 88-89 -0.8393582066290364 2316 90-91 -0.8605851924448586 2316 92-93 -0.8682899327881444 2316 94-95 -0.8593660544518222 2316 96-97 -0.8652952092551338 2316 98-99 -0.9532333870929683 2316 100 -1.0350517721446408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 15.0 8 63.0 9 117.0 10 317.0 11 816.0 12 737.0 13 1296.0 14 3247.0 15 9386.0 16 23668.0 17 40642.0 18 57495.0 19 79064.0 20 109728.0 21 155395.0 22 219674.0 23 307568.0 24 401966.0 25 453510.0 26 463758.0 27 449973.0 28 480015.0 29 554748.0 30 660991.0 31 803759.0 32 988407.0 33 1266255.0 34 1759479.0 35 2775717.0 36 4936921.0 37 9734654.0 38 1.1060139E7 39 2317548.0 40 2764.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.60652994827285 27.283953402837984 27.124712122234683 25.984804526654475 2 21.977731611637854 31.846182705849813 25.946878341863446 20.229207340648884 3 23.715291230531573 24.929947363687862 28.14656352499183 23.208197880788735 4 19.256486417988988 28.138612345136437 24.832880656130367 27.772020580744204 5 23.04838415076115 28.620907485355374 26.341286773085194 21.989421590798287 6 26.744142398203465 28.872167261318545 24.658881921539454 19.724808418938544 7 22.29727432557544 25.655299354194703 28.56525171890052 23.482174601329337 8 25.118470087312428 27.312963324472545 24.65705489494572 22.911511693269304 9 23.57236191816556 24.53026722544601 27.906998713254833 23.9903721431336 10-11 22.26415853386425 27.886275794973418 26.53648699226856 23.31307867889377 12-13 22.581772027360433 26.99399887816081 26.335315910595043 24.08891318388372 14-15 22.595313958443295 28.724726464457778 25.936423412740112 22.74353616435881 16-17 23.709864986473523 26.39498590123707 26.04336428926223 23.851784823027174 18-19 22.46442233357308 27.363829689017642 26.713674922666677 23.458073054742602 20-21 23.601014829772964 27.403917095166303 26.86865189265997 22.126416182400764 22-23 23.43584464660769 26.09092929402097 28.043978349660087 22.429247709711248 24-25 22.550282596438194 26.799954290333183 27.076982838447922 23.5727802747807 26-27 22.700082073971185 25.76682670355595 29.326462664196246 22.20662855827662 28-29 22.66714233341292 25.89233889812065 29.234979871303196 22.205538897163233 30-31 22.418205947631265 25.828341470882314 29.389580907718372 22.36387167376805 32-33 22.55733198059115 26.15734374407252 29.031678609022933 22.253645666313393 34-35 22.547000342267587 26.355267273042493 28.913027873026266 22.184704511663654 36-37 22.468946701664464 26.348283574877335 29.040289002777943 22.142480720680265 38-39 22.530590348998302 26.461181889497375 29.00962131887021 21.99860644263411 40-41 22.5602396556315 26.622846037745113 28.871291343871047 21.94562296275234 42-43 22.641109139167888 26.704293759642656 28.737051421104066 21.91754568008539 44-45 22.785102880400956 26.83881578410283 28.542629346123565 21.833451989372644 46-47 22.8495059044672 27.070844769682633 28.27732350895055 21.802325816899618 48-49 22.919103893900893 27.296580667459274 27.988397778417855 21.79591766022197 50-51 23.073902367046035 27.453673970602722 27.67344379728478 21.798979865066464 52-53 23.14516157682019 27.594737234757776 27.357602541047616 21.902498647374426 54-55 23.168187394049504 27.861837038545502 26.917344639306958 22.052630928098043 56-57 23.370859738461913 28.093067991310672 26.40722585587996 22.128846414347453 58-59 23.363898127591725 28.326268684928202 26.077306054466703 22.23252713301337 60-61 23.33562704026166 28.39265744521687 25.952075985524214 22.319639528997254 62-63 23.27291949171219 28.465336130473272 25.844231552049678 22.417512825764856 64-65 23.207869615622236 28.52034481717541 25.744653962831382 22.52713160437097 66-67 23.057538381715688 28.50027839592293 25.774048862959454 22.668134359401932 68-69 22.97898637677442 28.528304741847748 25.69495377137506 22.797755110002775 70-71 22.87235120586149 28.511061093646767 25.653307875497084 22.963279824994657 72-73 22.762375509153333 28.497702064590225 25.591197571666548 23.148724854589894 74-75 22.724267059129772 28.50917426761689 25.446077484666453 23.320481188586886 76-77 22.593339723998923 28.48512061465251 25.42948236531002 23.49205729603855 78-79 22.520786553691867 28.493035103749147 25.316637492676485 23.669540849882498 80-81 22.47806523210049 28.46200515235288 25.215119177373523 23.844810438173106 82-83 22.363975740580774 28.384857071623298 25.16984415178205 24.08132303601388 84-85 22.245304827258504 28.24552815155571 25.195927560916665 24.31323946026912 86-87 22.201620675504227 28.104274154555327 25.22468992923127 24.469415240709182 88-89 22.052958004991826 27.911040839581098 25.332146647506598 24.70385450792048 90-91 22.012044989901728 27.678028400947657 25.440270376437375 24.869656232713243 92-93 21.97545401277494 27.50140006135531 25.524595060255763 24.99855086561399 94-95 21.866630494487794 27.323213149324822 25.63668655563214 25.17346980055525 96-97 21.775228140766046 27.216022861386268 25.743515759203472 25.265233238644207 98-99 21.723428289072473 27.18778149850454 25.803052658751678 25.285737553671307 100 21.583403462978314 27.224912682017926 25.845359976508327 25.34632387849543 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27954.0 1 21841.0 2 12629.0 3 8611.5 4 8379.5 5 8592.0 6 9461.5 7 17757.0 8 23244.5 9 22426.0 10 23065.5 11 28812.5 12 82392.0 13 93175.5 14 56185.0 15 59853.0 16 67581.5 17 75412.5 18 82799.5 19 90991.0 20 102017.5 21 117904.5 22 139820.5 23 165589.5 24 190543.0 25 216559.0 26 246870.5 27 282753.0 28 332510.0 29 387835.0 30 434436.0 31 482985.5 32 535958.5 33 584842.5 34 633437.5 35 695935.5 36 765158.5 37 816667.5 38 874027.0 39 942081.5 40 994924.0 41 1053972.5 42 1128324.5 43 1188460.0 44 1240283.0 45 1303170.5 46 1367506.5 47 1429428.0 48 1496847.0 49 1573675.0 50 1652585.0 51 1716162.0 52 1735474.0 53 1704683.0 54 1633427.0 55 1523728.0 56 1386697.0 57 1230970.5 58 1060194.5 59 883240.0 60 711158.0 61 558955.0 62 433647.0 63 330437.0 64 249109.0 65 187524.5 66 140244.5 67 105026.0 68 79380.0 69 61939.5 70 49074.0 71 38566.0 72 30751.5 73 23484.0 74 18102.0 75 15011.0 76 11199.5 77 6712.5 78 4036.0 79 2710.5 80 1601.0 81 946.5 82 557.0 83 287.0 84 162.5 85 118.0 86 84.0 87 53.0 88 36.0 89 21.5 90 10.0 91 5.5 92 5.0 93 2.0 94 1.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13513516208143644 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0034982698830842564 26-27 0.02107436541608649 28-29 0.029710991810733407 30-31 0.03816441703943326 32-33 0.031549234802379034 34-35 0.016152859264216262 36-37 0.008477104290965126 38-39 0.005879885040395982 40-41 0.0014942734555817682 42-43 0.007903821730858693 44-45 0.007920023194513875 46-47 0.007591008855670183 48-49 0.008417283502084455 50-51 0.011098002603799537 52-53 0.020013792679889588 54-55 0.025668103495548038 56-57 0.03053602019071266 58-59 0.03175611503059136 60-61 0.025142179059972136 62-63 0.0208973955823145 64-65 0.022315646785360417 66-67 0.019676054476000796 68-69 0.01400055713094711 70-71 0.01345718496528101 72-73 0.011424524409773202 74-75 0.009869183898875748 76-77 0.004847976432204402 78-79 0.008308858322238238 80-81 0.012835298014209033 82-83 0.006146586057488974 84-85 0.00818423167873684 86-87 0.007734329495696792 88-89 0.007637120713765701 90-91 0.008271470329187819 92-93 0.0026283759114444946 94-95 0.0014980122548868102 96-97 0.002941188786633005 98-99 6.991554700428456E-4 100 2.2183542543248935E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.0119832E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.13325422446997 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18966819852561 33.93554409857601 2 11.98297402933442 10.816612264623434 3 4.430093698472303 5.998336353941188 4 2.218340827511053 4.004837620983097 5 1.397253316004887 3.153129456361612 6 0.9193676569283536 2.48964325115416 7 0.6608514262814286 2.0878462798874304 8 0.49494993764423734 1.787096109126634 9 0.36991329881249446 1.5025851860684973 >10 2.0582528018976207 16.630429323327693 >50 0.16958086630193628 5.358840019917465 >100 0.10668282478902137 8.67733532906684 >500 0.0014715737861922804 0.443253219586809 >1k 5.167437570773287E-4 0.380519440622311 >5k 2.7599987800504744E-5 0.08589691842467834 >10k+ 5.519996553950819E-5 2.6480951283321335 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 743616 1.853487322678719 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83204 0.20738870491780723 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 55880 0.13928273677716296 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.018364982186366084 7.4775986100839105E-6 0.0 0.0 7.4775986100839105E-6 2 0.018372459784976168 7.4775986100839105E-6 0.0 2.49253287002797E-6 2.2432795830251733E-5 3 0.018377444850716223 7.4775986100839105E-6 0.0 7.4775986100839105E-6 2.2432795830251733E-5 4 0.018379937383586252 1.246266435013985E-5 0.0 9.97013148011188E-6 3.4895460180391586E-5 5 0.018379937383586252 1.7447730090195793E-5 0.0 1.246266435013985E-5 4.735812453053143E-5 6 0.018384922449326307 1.7447730090195793E-5 0.0 1.246266435013985E-5 4.735812453053143E-5 7 0.018384922449326307 2.49253287002797E-5 0.0 1.246266435013985E-5 5.732825601064331E-5 8 0.018389907515066365 2.7417861570307674E-5 0.0 1.246266435013985E-5 5.9820788880671284E-5 9 0.01839240004793639 3.2402927310363614E-5 0.0 1.4955197220167821E-5 6.480585462072723E-5 10-11 0.018396138847241433 3.863425948543354E-5 0.0 1.4955197220167821E-5 7.60222525358531E-5 12-13 0.018423556708811743 4.4865591660503466E-5 0.0 1.7447730090195793E-5 9.97013148011188E-5 14-15 0.018447235771077007 4.98506574005594E-5 0.0 1.7447730090195793E-5 1.1839531132632859E-4 16-17 0.018475899899082326 5.358945670560136E-5 0.0 1.8693996525209775E-5 1.3708930785153836E-4 18-19 0.018500825227782607 6.106705531568528E-5 0.0 1.994026296022376E-5 1.5453703794173415E-4 20-21 0.018575601213883446 7.352971966582511E-5 0.0 2.2432795830251733E-5 1.595221036817901E-4 22-23 0.019222413493655707 8.723865045097895E-5 0.0 2.49253287002797E-5 1.8693996525209777E-4 24-25 0.02217980374394389 1.0219384767114678E-4 0.0 2.741786157030767E-5 2.156040932574194E-4 26-27 0.03452781158206246 1.096714462812307E-4 0.0 3.115666087534963E-5 2.3554435621764319E-4 28-29 0.07250528865624363 1.2213411063137053E-4 0.0 3.2402927310363614E-5 2.5049955343781104E-4 30-31 0.1608418998364699 1.333505085464964E-4 0.0 3.36491937453776E-5 2.679472835280068E-4 32-33 0.3268308800495475 1.345967749815104E-4 0.0 4.112679235546151E-5 2.7916368144313263E-4 34-35 0.589399277644034 1.4082810715658034E-4 0.0 4.4865591660503466E-5 3.0284274370839836E-4 36-37 0.9552407896423893 1.4830570576666424E-4 0.0 4.98506574005594E-5 3.252755395386501E-4 38-39 1.4222218079078697 1.5329077150672015E-4 0.0 5.2343190270587375E-5 3.414770031938319E-4 40-41 1.9898587810636894 1.5453703794173415E-4 0.0 5.732825601064331E-5 3.5144713467394383E-4 42-43 2.657660680134453 1.595221036817901E-4 0.0 6.231332175069926E-5 3.601709997190417E-4 44-45 3.412075603905819 1.6949223516190198E-4 0.0 6.72983874907552E-5 3.776187298092375E-4 46-47 4.248477411371015 1.8693996525209777E-4 0.0 6.979092036078317E-5 3.913276605943913E-4 48-49 5.1656023883649365 1.919250309921537E-4 0.0 6.979092036078317E-5 4.012977920745032E-4 50-51 6.1550519453820245 1.9691009673220966E-4 0.0 7.228345323081114E-5 4.1251418998962907E-4 52-53 7.209440707528387 2.0438769534229356E-4 0.0 7.726851897086707E-5 4.287156536448109E-4 54-55 8.31612654808724 2.1186529395237746E-4 0.0 7.726851897086707E-5 4.3494698581988077E-4 56-57 9.46265552657349 2.3180555691260123E-4 0.0 8.100731827590904E-5 4.4740965017002064E-4 58-59 10.647915973327105 2.4426822126274105E-4 0.0 8.474611758095099E-5 4.661036466952304E-4 60-61 11.864582334243075 2.554846191778669E-4 0.0 9.097744975602091E-5 4.8355137678542624E-4 62-63 13.110088546731701 2.7043981639803476E-4 0.0 9.471624906106286E-5 4.99752840440608E-4 64-65 14.371922095785447 2.754248821380907E-4 0.0 9.471624906106286E-5 5.072304390506919E-4 66-67 15.648323253198068 2.853950136182026E-4 0.0 9.471624906106286E-5 5.209393698358458E-4 68-69 16.930692780567973 2.941188786633005E-4 0.0 9.471624906106286E-5 5.396333663610555E-4 70-71 18.220269466731565 2.966114115333285E-4 0.0 9.471624906106286E-5 5.508497642761814E-4 72-73 19.511781604668734 3.040890101434124E-4 0.0 1.0344011410616077E-4 5.620661621913072E-4 74-75 20.80666364704618 3.065815430134403E-4 0.0 1.096714462812307E-4 5.670512279313633E-4 76-77 22.098068606069937 3.1032034231848226E-4 0.0 1.1216397915125867E-4 5.720362936714192E-4 78-79 23.381539832968393 3.1530540805853823E-4 0.0 1.1216397915125867E-4 5.85745224456573E-4 80-81 24.65992504654556 3.252755395386501E-4 0.0 1.1465651202128662E-4 6.019466881117548E-4 82-83 25.92977084251001 3.40230736758818E-4 0.0 1.1590277845630062E-4 6.169018853319226E-4 84-85 27.189663954724438 3.5144713467394383E-4 0.0 1.1839531132632858E-4 6.306108161170765E-4 86-87 28.440664955925037 3.576784668490137E-4 0.0 1.1964157776134257E-4 6.331033489871044E-4 88 29.37113246137222 3.6141726615405566E-4 0.0 1.1964157776134257E-4 6.331033489871044E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 123565 0.0 70.56998 1 GCAGTGG 139430 0.0 62.302235 3 AGCAGTG 144135 0.0 60.65321 2 GTGGTAT 156410 0.0 55.211178 6 AGTGGTA 157895 0.0 54.754417 5 CAGTGGT 164175 0.0 52.840282 4 TGGTATC 169150 0.0 50.963898 7 GGTATCA 172095 0.0 50.258335 8 GTATCAA 195330 0.0 44.26554 9 AGTACTT 175400 0.0 24.426147 20-21 GAGTACT 164330 0.0 23.669085 20-21 TACTTTT 187890 0.0 22.875977 22-23 GTACTTT 171770 0.0 22.6174 22-23 ATCAACG 216885 0.0 22.392258 10-11 CAACGCA 221595 0.0 21.884502 12-13 ACGCAGA 229535 0.0 21.131575 14-15 AGAGTAC 229090 0.0 20.867731 18-19 TCAACGC 217605 0.0 19.865005 12-13 TATCAAC 218705 0.0 19.810211 10-11 GCAGAGT 252135 0.0 19.2943 16-17 >>END_MODULE