FastQCFastQC Report
Thu 26 May 2016
SRR1660644_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660644_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46901100
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12558222.6775960478538883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1937400.075.03711
GGTATCA1916450.074.621988
AGTGGTA1927500.074.3166965
GTGGTAT1935300.073.8853156
TGGTATC1956700.072.897387
GCAGTGG2010650.072.131573
GTATCAA1990100.071.724519
CAGTGGT2086150.068.9452744
AGCAGTG2125700.068.483142
CAACGCA1936650.039.31222512-13
ATCAACG1968250.038.7811910-11
ACGCAGA1989950.038.19042214-15
AGAGTAC1952450.038.02392218-19
GAGTACT1772800.037.84055720-21
AGTACTT1906550.037.42565520-21
TCAACGC1932950.037.0284412-13
AACGCAG1961500.036.34117514-15
GTACTTT1868150.035.80471422-23
CGCAGAG2015050.035.2043216-17
TATCAAC2033400.035.06765710-11
Produced by FastQC (version 0.11.5)