FastQCFastQC Report
Thu 26 May 2016
SRR1660644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46901100
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12936602.758272194042357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1941500.075.1509251
GGTATCA1933950.074.7852258
AGTGGTA1942950.074.470255
GTGGTAT1954450.073.9743656
TGGTATC1971850.073.145257
GCAGTGG2020850.072.0762563
GTATCAA2016150.071.563699
CAGTGGT2109350.068.865084
AGCAGTG2148350.068.196782
CAACGCA1954000.039.2370612-13
ATCAACG1988550.038.6356810-11
ACGCAGA2016900.038.01572414-15
GAGTACT1795450.037.9554620-21
AGAGTAC1995900.037.8448818-19
AGTACTT1926550.037.67688820-21
TCAACGC1950850.036.8892312-13
AACGCAG1984400.036.14714414-15
GTACTTT1899200.035.80300522-23
CGCAGAG2043350.035.0330216-17
TATCAAC2066200.034.9378410-11
Produced by FastQC (version 0.11.5)