FastQCFastQC Report
Thu 26 May 2016
SRR1660643_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660643_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34010060
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8289772.4374464496681276No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1522540.4476734236869915Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1208400.076.778378
GTGGTAT1214600.076.462256
AGTGGTA1230350.075.64475
TGGTATC1227150.075.523437
GTATCAA1230350.075.224879
AAGCAGT1298600.072.8271
GCAGTGG1352150.069.7318043
CAGTGGT1348150.069.332864
AGCAGTG1436400.065.965622
CAACGCA1188550.041.44532812-13
ATCAACG1208850.040.91210-11
ACGCAGA1228650.040.0529814-15
AGAGTAC1213250.039.6994718-19
TCAACGC1179000.039.2771312-13
GAGTACT1136950.038.4985820-21
AGTACTT1221600.038.235620-21
AACGCAG1207400.038.1818114-15
TATCAAC1258400.036.78744510-11
CGCAGAG1256600.036.53667416-17
GTACTTT1208200.036.21865522-23
Produced by FastQC (version 0.11.5)