##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660643_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34010060 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18922951032724 34.0 31.0 34.0 30.0 34.0 2 32.36115966863922 34.0 31.0 34.0 30.0 34.0 3 32.38194269577884 34.0 31.0 34.0 30.0 34.0 4 35.77578231264514 37.0 35.0 37.0 35.0 37.0 5 35.77227429178308 37.0 35.0 37.0 35.0 37.0 6 35.79050504468384 37.0 35.0 37.0 35.0 37.0 7 35.75477596922793 37.0 35.0 37.0 35.0 37.0 8 35.772476143823326 37.0 35.0 37.0 35.0 37.0 9 37.46556395372428 39.0 38.0 39.0 35.0 39.0 10-11 37.3768308847441 39.0 37.0 39.0 34.5 39.0 12-13 37.3836441335299 39.0 37.0 39.0 34.0 39.0 14-15 38.764964586948686 40.0 38.0 41.0 35.0 41.0 16-17 38.702089778730176 40.0 38.0 41.0 34.0 41.0 18-19 38.57747667895911 40.0 38.0 41.0 34.0 41.0 20-21 38.54038406577348 40.0 38.0 41.0 34.0 41.0 22-23 38.43912584982208 40.0 38.0 41.0 34.0 41.0 24-25 38.3847355164913 40.0 38.0 41.0 34.0 41.0 26-27 38.303869707962875 40.0 38.0 41.0 34.0 41.0 28-29 38.18372341889429 40.0 38.0 41.0 33.0 41.0 30-31 37.984224388313336 40.0 38.0 41.0 33.0 41.0 32-33 37.97911065137786 40.0 38.0 41.0 33.0 41.0 34-35 38.047905575585574 40.0 38.0 41.0 33.0 41.0 36-37 37.91393034884384 40.0 38.0 41.0 33.0 41.0 38-39 37.65296634289972 40.0 37.0 41.0 32.0 41.0 40-41 37.49911383572979 40.0 37.0 41.0 32.0 41.0 42-43 37.42859342206394 40.0 37.0 41.0 31.5 41.0 44-45 37.53827133795119 40.0 37.0 41.0 32.0 41.0 46-47 37.408815127053586 40.0 36.5 41.0 31.5 41.0 48-49 37.26085125989192 40.0 36.0 41.0 31.0 41.0 50-51 36.131195828528384 38.5 34.5 40.0 29.5 40.5 52-53 36.21390400369773 38.5 35.0 40.0 30.0 40.5 54-55 36.628127677516595 39.0 35.0 41.0 30.0 41.0 56-57 36.380484142045034 39.0 35.0 41.0 29.0 41.0 58-59 36.13577434147426 39.0 35.0 41.0 28.0 41.0 60-61 35.86257451471711 39.0 35.0 41.0 28.0 41.0 62-63 35.58074815216439 38.0 35.0 40.5 28.0 41.0 64-65 35.24800776593749 37.0 34.5 40.0 27.0 41.0 66-67 34.823828243760815 37.0 34.0 40.0 26.0 41.0 68-69 34.449713452431425 36.0 34.0 39.0 26.0 41.0 70-71 34.113742301542544 35.5 34.0 39.0 26.0 41.0 72-73 33.73801248806971 35.0 33.0 38.5 26.0 40.0 74-75 33.35331360779722 35.0 33.0 37.0 25.0 39.5 76-77 32.98462893038119 35.0 33.0 37.0 24.5 39.0 78-79 32.64203544480662 35.0 33.0 36.0 24.0 39.0 80-81 32.314983493119385 35.0 32.0 36.0 24.0 37.5 82-83 31.993195954373498 35.0 32.0 35.0 23.0 37.0 84-85 31.736953742510305 35.0 32.0 35.0 22.5 37.0 86-87 31.478884027255468 35.0 31.0 35.0 20.5 36.0 88-89 31.26747609383812 35.0 31.0 35.0 20.0 36.0 90-91 31.0405500901792 34.0 31.0 35.0 20.0 36.0 92-93 30.873404178057903 34.0 31.0 35.0 19.5 35.0 94-95 30.685145042378636 34.0 30.5 35.0 19.0 35.0 96-97 30.49147734523256 34.0 30.0 35.0 18.0 35.0 98-99 30.341512922941035 34.0 30.0 35.0 18.0 35.0 100 30.201633722492698 34.0 30.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2577426216546428 1101 2 0.2652882827210661 1101 3 0.26143414913394736 1101 4 0.22780471127379087 1101 5 0.219707031717995 1101 6 0.20269970825122385 1101 7 0.21115005935235587 1101 8 0.2255039430826855 1101 9 0.29460828775398795 1101 10-11 0.3132950003810677 1101 12-13 0.2777642533977627 1101 14-15 0.33470522779681033 1101 16-17 0.3245711814292136 1101 18-19 0.29917024870110964 1101 20-21 0.3514791820912819 1101 22-23 0.31474774898725144 1101 24-25 0.28597311065806963 1101 26-27 0.2683413897349354 1101 28-29 0.23260793242580746 1101 30-31 0.29596575520555035 1101 32-33 0.2665769914354712 1101 34-35 0.24608515982400547 1101 36-37 0.2369958214759862 1101 38-39 0.25239998205713476 1101 40-41 0.1935331979648538 1101 42-43 0.24603251360485956 1101 44-45 0.2593382544791112 1101 46-47 0.2690740776635039 1101 48-49 0.24007650414834103 1101 50-51 0.16170600317294515 1101 52-53 0.2782535928152896 1101 54-55 0.3694160964178579 1101 56-57 0.36186672330715197 1101 58-59 0.3395310719216553 1101 60-61 0.3218412825042307 1101 62-63 0.3045417925511842 1101 64-65 0.2614653114873491 1101 66-67 0.25609880497952275 1101 68-69 0.2636772866269652 1101 70-71 0.21774390131601962 1101 72-73 0.2196195414304256 1101 74-75 0.2001821531374759 1101 76-77 0.19729360092097892 1101 78-79 0.2086900244927392 1101 80-81 0.16064322180953639 1101 82-83 0.1302418945416619 1101 84-85 0.09157889789589291 1101 86-87 0.052564064031482616 1101 88-89 0.014609905329471928 1101 90-91 0.046111882641820756 1101 92-93 0.01976434044614095 1101 94-95 -0.05599691274066387 1101 96-97 0.016173042898149248 1101 98-99 -0.10547063420734304 1101 100 -0.1744051608180257 1102 1 0.15909602052457217 1102 2 0.1938517039024248 1102 3 0.15018097560228938 1102 4 0.13871595776933532 1102 5 0.1726742814848805 1102 6 0.16752023666978033 1102 7 0.1489552338556095 1102 8 0.1776473544143684 1102 9 0.22735197928714967 1102 10-11 0.2312122624308941 1102 12-13 0.20431612910215335 1102 14-15 0.1852624583289071 1102 16-17 0.2091856401546366 1102 18-19 0.20271491041429357 1102 20-21 0.23751044110210273 1102 22-23 0.2133256716095886 1102 24-25 0.1963969529055376 1102 26-27 0.17400293787009247 1102 28-29 0.17115427519568982 1102 30-31 0.20856233301701366 1102 32-33 0.15573922333150847 1102 34-35 0.12199069150111086 1102 36-37 0.12160476754376504 1102 38-39 0.11439924112803368 1102 40-41 0.09650244761768079 1102 42-43 0.15810732366004032 1102 44-45 0.08701476895529225 1102 46-47 0.1758705568530985 1102 48-49 0.19283064321444243 1102 50-51 0.1960396183278661 1102 52-53 0.20680842030586177 1102 54-55 0.2181241375692693 1102 56-57 0.21265445619831524 1102 58-59 0.2131520622662606 1102 60-61 0.18142412583418377 1102 62-63 0.14832892068871217 1102 64-65 0.10876388830733674 1102 66-67 0.12760495729678212 1102 68-69 0.18104744891929414 1102 70-71 0.07384248896502044 1102 72-73 0.09594484600520303 1102 74-75 0.05999224446764373 1102 76-77 0.14154106602249072 1102 78-79 0.10128560664934838 1102 80-81 0.06347800954882388 1102 82-83 0.0408581761492286 1102 84-85 -0.02235594103660432 1102 86-87 -0.01198039503919901 1102 88-89 -0.008656366429818263 1102 90-91 -0.02351102899790547 1102 92-93 -0.06759351115200474 1102 94-95 -0.1103122500702689 1102 96-97 -0.08306846211780439 1102 98-99 -0.1905672640953 1102 100 -0.2795504177262593 1103 1 0.07557267691819902 1103 2 0.14780038957388797 1103 3 0.1261676805316725 1103 4 0.13966769818456015 1103 5 0.1401032469286818 1103 6 0.13576851957758862 1103 7 0.1017377947397975 1103 8 0.1360608099608811 1103 9 0.12598251222994605 1103 10-11 0.16566063153724286 1103 12-13 0.16506171370431844 1103 14-15 0.17910651371402508 1103 16-17 0.20813881943480794 1103 18-19 0.24119035700387315 1103 20-21 0.21405623909106453 1103 22-23 0.2077496066181581 1103 24-25 0.21474030144141665 1103 26-27 0.17674809697305705 1103 28-29 0.18198049958166962 1103 30-31 0.2530913742169574 1103 32-33 0.19029735419391614 1103 34-35 0.16204099495354995 1103 36-37 0.1476871631925789 1103 38-39 0.14174374977775983 1103 40-41 0.11768244463360844 1103 42-43 0.1452692676692422 1103 44-45 0.053943960205437236 1103 46-47 0.13500730736905098 1103 48-49 0.17633030716061882 1103 50-51 0.109367202691935 1103 52-53 0.19732530297660134 1103 54-55 0.23887163993241955 1103 56-57 0.2797645908794735 1103 58-59 0.25971639447622863 1103 60-61 0.24774548361135373 1103 62-63 0.24056273336273648 1103 64-65 0.11083796979767868 1103 66-67 0.16868857692481498 1103 68-69 0.22357476067626436 1103 70-71 0.17930461389055807 1103 72-73 0.19922706795596667 1103 74-75 0.1769299442448542 1103 76-77 0.19524838780071008 1103 78-79 0.2140664639426788 1103 80-81 0.12268746252754426 1103 82-83 0.09339864895551386 1103 84-85 0.050808608319066195 1103 86-87 0.09206430123562725 1103 88-89 0.09306884081137312 1103 90-91 0.0889552777212721 1103 92-93 0.01803437201623126 1103 94-95 -0.0052244188113270695 1103 96-97 0.04698743634680724 1103 98-99 0.003997338106998427 1103 100 -0.07756754311670733 1104 1 0.10660016475399203 1104 2 0.11762646295166945 1104 3 0.09125297509079644 1104 4 0.10357243414362927 1104 5 0.09946627813470599 1104 6 0.07691666020851784 1104 7 0.08929343459759309 1104 8 0.08257851478884248 1104 9 0.09108923380201617 1104 10-11 0.11826680898888497 1104 12-13 0.11967495747200019 1104 14-15 0.07341157932234665 1104 16-17 0.09892947310033406 1104 18-19 0.09277926326762298 1104 20-21 0.09895965000251294 1104 22-23 0.07583532389503489 1104 24-25 0.09313340180976581 1104 26-27 0.10169448716401774 1104 28-29 0.08854643938779816 1104 30-31 0.09311629193310722 1104 32-33 0.07718037358261398 1104 34-35 0.07258836796020773 1104 36-37 0.0516218899680112 1104 38-39 -0.03132378301602756 1104 40-41 0.05511842027468816 1104 42-43 0.07263448683411156 1104 44-45 0.025175459123182975 1104 46-47 0.04430822286018099 1104 48-49 0.08007208589561543 1104 50-51 0.021091971224407757 1104 52-53 0.12333705291091945 1104 54-55 0.18836205260117111 1104 56-57 0.2118852219200349 1104 58-59 0.1946498133399075 1104 60-61 0.20233525050973356 1104 62-63 0.16479241545977175 1104 64-65 0.14692844367488078 1104 66-67 0.15675936692755954 1104 68-69 0.19694409343863128 1104 70-71 0.14727954259844722 1104 72-73 0.09997146175287952 1104 74-75 0.1532401887863628 1104 76-77 0.1578561005615029 1104 78-79 0.08883160977499216 1104 80-81 0.05484827945384296 1104 82-83 0.01522082977428596 1104 84-85 0.07427799782063715 1104 86-87 0.030076975430478825 1104 88-89 0.04579448982906342 1104 90-91 0.06773819822510418 1104 92-93 -0.0074884074842564985 1104 94-95 7.76904975165138E-4 1104 96-97 -0.007673728916881117 1104 98-99 -0.11431392734793988 1104 100 -0.10744137486184968 1105 1 0.03514869206195925 1105 2 0.03628161275975117 1105 3 0.053858936751346675 1105 4 0.06303100182879007 1105 5 0.05238191027465433 1105 6 0.002391970885263106 1105 7 0.05396335469160363 1105 8 0.06117267572617635 1105 9 0.03057417895931991 1105 10-11 0.03891382652534503 1105 12-13 0.04845590767628494 1105 14-15 0.051751019995343484 1105 16-17 0.0014970441621997566 1105 18-19 0.05540219285459358 1105 20-21 0.027348727441697918 1105 22-23 0.03908760863520655 1105 24-25 0.058236175482207386 1105 26-27 0.039025396256612055 1105 28-29 0.015062818077993256 1105 30-31 0.005781676648489054 1105 32-33 0.005708964002629102 1105 34-35 0.006227513475138835 1105 36-37 0.02467834230737509 1105 38-39 0.02531524099426008 1105 40-41 -0.01805032671131812 1105 42-43 0.03515894505309092 1105 44-45 0.048127753589618294 1105 46-47 0.036243728579748336 1105 48-49 0.05496789258874202 1105 50-51 -0.028345623558927002 1105 52-53 0.0767209458302176 1105 54-55 0.09286434374595842 1105 56-57 0.1611392056941625 1105 58-59 0.1254876687484483 1105 60-61 0.18313740196712303 1105 62-63 0.08856502232281116 1105 64-65 0.11228557232821856 1105 66-67 0.1293202736144039 1105 68-69 0.07530047051958633 1105 70-71 0.022498145158813543 1105 72-73 0.02201392865985241 1105 74-75 0.0033484344290357626 1105 76-77 0.026835395937261808 1105 78-79 0.0314362681081235 1105 80-81 0.059836987959094756 1105 82-83 0.04254904181046726 1105 84-85 0.031456345657773 1105 86-87 0.07926391378852671 1105 88-89 0.04090555282847319 1105 90-91 -0.012020492052968734 1105 92-93 -0.04782382334467883 1105 94-95 -0.02725538967233021 1105 96-97 0.06920143335517537 1105 98-99 -0.05269809207995024 1105 100 -0.10737759277419556 1106 1 -0.0197503611139922 1106 2 -0.025935432465267638 1106 3 -0.005341176120168711 1106 4 0.00446773402144629 1106 5 0.007358319325170726 1106 6 -0.019143849763892717 1106 7 0.023935043164470926 1106 8 0.04097405560467848 1106 9 0.007927145107395006 1106 10-11 0.0429193928563123 1106 12-13 0.03289669951764296 1106 14-15 0.027088410021541165 1106 16-17 0.0023243476494130277 1106 18-19 -0.02642406700520894 1106 20-21 0.0031557008855713775 1106 22-23 -0.02839930784760014 1106 24-25 0.01620667569349621 1106 26-27 -0.011626568580929586 1106 28-29 -0.022025284215267504 1106 30-31 0.019829296217686476 1106 32-33 0.011600807985963968 1106 34-35 -0.021482150761464425 1106 36-37 -0.0569826871553758 1106 38-39 -0.07954139646907521 1106 40-41 -0.0146040421153657 1106 42-43 0.022367406844374216 1106 44-45 0.004598539039001537 1106 46-47 -0.0202198260821973 1106 48-49 -0.0046535829440585985 1106 50-51 -0.1972791196211645 1106 52-53 -0.08931731827752287 1106 54-55 0.01100067849670694 1106 56-57 0.005362007271919822 1106 58-59 0.045426630061882634 1106 60-61 0.0911632736698067 1106 62-63 0.054167011450495295 1106 64-65 0.05556155713649247 1106 66-67 0.09839463116028213 1106 68-69 0.10641470504238981 1106 70-71 0.06563438571671298 1106 72-73 -0.06439899884613709 1106 74-75 -0.06452987894943618 1106 76-77 0.02493616066370663 1106 78-79 -0.018487500712303984 1106 80-81 0.013466527550342278 1106 82-83 0.07065168833062074 1106 84-85 0.025674179471863567 1106 86-87 0.0369844933977177 1106 88-89 0.013710035135126475 1106 90-91 -0.02854761083871793 1106 92-93 -0.051780112301059944 1106 94-95 0.005263908915441817 1106 96-97 0.005874040856859608 1106 98-99 -0.0786010981152252 1106 100 -0.13964017367413817 1107 1 -0.046397540717194374 1107 2 -0.008140746198833426 1107 3 1.8562977381009205E-4 1107 4 0.02611522908964048 1107 5 -0.006859585864269491 1107 6 -0.00808510535871676 1107 7 0.007139522081246241 1107 8 -0.05719728653009071 1107 9 -0.04900124051338395 1107 10-11 0.001883707837116333 1107 12-13 -0.008331958306072806 1107 14-15 -0.05157715892427461 1107 16-17 -0.05592972254351736 1107 18-19 -0.043201013961137846 1107 20-21 0.018807964181327463 1107 22-23 -0.03508081876531577 1107 24-25 -0.030014859363767243 1107 26-27 -0.00998844923306308 1107 28-29 -0.07931389785867538 1107 30-31 -0.052501793356185544 1107 32-33 -0.11614663314056628 1107 34-35 -0.07977224127144211 1107 36-37 -0.08584636692936698 1107 38-39 -0.08741395318914158 1107 40-41 -0.12341473862989005 1107 42-43 -0.1038076732135167 1107 44-45 -0.10696176487870446 1107 46-47 -0.14916137679814767 1107 48-49 -0.17069799040412903 1107 50-51 -0.30367926231105713 1107 52-53 -0.11387269824687962 1107 54-55 -0.033781190128152616 1107 56-57 0.008036576526258443 1107 58-59 0.019492118197675268 1107 60-61 0.055838325100666 1107 62-63 0.054150159967669254 1107 64-65 0.026467209992695473 1107 66-67 0.009348492546727982 1107 68-69 -0.03423745208684892 1107 70-71 1.6502157036057952E-4 1107 72-73 -0.0032850214278710155 1107 74-75 -0.013613382998975965 1107 76-77 0.023706409207100876 1107 78-79 0.015314501091090449 1107 80-81 -0.010794385291610809 1107 82-83 -0.021001404324927364 1107 84-85 -0.08911353700557356 1107 86-87 -0.013893194437994083 1107 88-89 -0.010217579792566767 1107 90-91 0.0154462663169177 1107 92-93 -0.12682623387216552 1107 94-95 -0.14004496692302126 1107 96-97 -0.029443740709055533 1107 98-99 -0.12448286280641696 1107 100 -0.14766836484720258 1108 1 -0.10393246088399621 1108 2 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-1.1686435473828283 2316 52-53 -1.3895491597909881 2316 54-55 -1.4302473062445742 2316 56-57 -1.5853715855387307 2316 58-59 -1.714385874048702 2316 60-61 -1.266114842891966 2316 62-63 -1.0721059640072852 2316 64-65 -1.212108835234467 2316 66-67 -1.2678250422730528 2316 68-69 -1.1797041096572443 2316 70-71 -0.9593934361071561 2316 72-73 -1.04119305805974 2316 74-75 -1.0665959376239798 2316 76-77 -1.4546561356262728 2316 78-79 -1.277545448475621 2316 80-81 -1.1123124660101027 2316 82-83 -1.4033048093746459 2316 84-85 -1.0468560567618574 2316 86-87 -1.0411521532946644 2316 88-89 -1.4138331572688507 2316 90-91 -1.6080397799497455 2316 92-93 -1.1739495233130164 2316 94-95 -1.0439361083918506 2316 96-97 -1.6703788632094714 2316 98-99 -1.6381659746331607 2316 100 -1.2494907386367196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 563.0 3 1364.0 4 2461.0 5 7730.0 6 6988.0 7 6446.0 8 13850.0 9 20230.0 10 23562.0 11 25438.0 12 29638.0 13 36276.0 14 44830.0 15 55923.0 16 72334.0 17 90716.0 18 102606.0 19 117109.0 20 136371.0 21 163749.0 22 205110.0 23 277137.0 24 369076.0 25 441692.0 26 475512.0 27 487693.0 28 446495.0 29 479768.0 30 568167.0 31 697556.0 32 880026.0 33 1149433.0 34 1606319.0 35 2450062.0 36 4287824.0 37 7756007.0 38 8359263.0 39 2111042.0 40 3694.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.61368844606111 30.827668466489914 27.232658683340627 24.32598440410835 2 23.34717576045137 30.006370373372587 26.36825158148291 20.27820228469313 3 24.198712377915324 25.626896275763368 26.598682921547066 23.57570842477424 4 21.13870337413132 27.190334582941894 25.180623125300432 26.49033891762635 5 23.254729609242926 28.69372436643303 25.980174434114822 22.071371590209218 6 26.90867142465534 26.6669128959427 25.390856138604654 21.0335595407973 7 23.18748570461003 25.618200444995747 28.715014927783617 22.479298922610603 8 25.2560125483412 26.753060257790512 25.532741545144038 22.458185648724253 9 24.67226399841127 25.50107266967284 26.971951221448524 22.85471211046736 10-11 22.60719100148318 27.899665380137655 27.111137829263242 22.38200578911592 12-13 22.53310373751671 26.27207304221524 27.06861107400401 24.126212146264034 14-15 22.529181091378245 29.067364161822162 25.997539795994406 22.405914950805187 16-17 24.110888691642508 26.00425390840117 25.813431713717343 24.07142568623898 18-19 22.619801817050554 27.46886729398913 26.065850685705094 23.84548020325522 20-21 23.81292601023563 27.792530517817127 26.04613387949812 22.34840959244912 22-23 24.28420105868199 26.136082127800563 27.200845452633764 22.378871360883682 24-25 22.716789953029785 27.585553353305475 25.69776219443486 23.999894499229878 26-27 22.513802651015034 26.44970793821083 28.614381373087276 22.422108037686854 28-29 22.77262066990734 26.279527118689167 28.566840497926954 22.381011713476536 30-31 22.75864448760234 25.929426441768182 28.833923067437468 22.47800600319201 32-33 22.810523794448855 26.0676588418218 28.691579670127947 22.43023769360139 34-35 22.816355030303068 26.435973939563358 28.360010738930523 22.387660291203048 36-37 22.524511558210126 26.380253738062947 28.660957948076955 22.434276755649975 38-39 22.573636309105407 26.12233455807148 28.686593870713118 22.617435262109993 40-41 22.867608240032347 26.18581566044032 28.380837233994345 22.56573886553299 42-43 22.90328190432881 26.191726555493545 28.566596321048817 22.338395219128827 44-45 23.190143944981884 26.263439955643726 28.28312881227186 22.263287287102536 46-47 22.751624945500918 26.364587644418613 28.43299048280099 22.450796927279477 48-49 23.078680697041012 26.332651024954544 28.085712128250666 22.502956149753768 50-51 23.18374608430986 26.35592944884001 28.02845680950716 22.431867657342973 52-53 23.08489551358872 26.718506683998598 28.06706543987939 22.12953236253329 54-55 23.31538671639133 26.5845052777806 27.650155717269058 22.449952288559007 56-57 23.644462219372112 27.099186112691164 27.09488563201311 22.161466035923613 58-59 23.830792385046283 27.36248970302474 26.742039824065984 22.06467808786299 60-61 24.013250730690352 27.698851347983776 26.293129117281556 21.994768804044316 62-63 24.12365763040102 27.805264648574628 26.189548575423157 21.8815291456012 64-65 24.28398287494781 27.894136410243554 26.098151241469747 21.723729473338892 66-67 24.438796152538846 27.759547481052753 26.161453240981192 21.640203125427213 68-69 24.591851258502032 27.64027866956843 26.263089014481267 21.504781057448273 70-71 24.78670176658554 27.51926062057687 26.311821939656838 21.382215673180756 72-73 24.97376546978389 27.429796771021742 26.28147277004359 21.31496498915078 74-75 25.1988924579039 27.407480141521724 26.2278301860058 21.16579721456858 76-77 25.469013596924782 27.44445826050766 26.030524935605282 21.056003206962284 78-79 25.722904228326637 27.414359995796193 25.859130454237555 21.00360532163961 80-81 25.938421685247793 27.44960316947494 25.685005756395423 20.926969388881844 82-83 26.13216111488888 27.488499956327317 25.51397963653585 20.86535929224795 84-85 26.276281476093054 27.438227291447536 25.43339508346246 20.852096148996953 86-87 26.463577318878258 27.371093009760965 25.370122322099775 20.795207349261 88-89 26.612110357318876 27.351463616805834 25.309939646446196 20.7264863794291 90-91 26.745481380820486 27.289368705576837 25.25789443702489 20.707255476577778 92-93 26.813786379543703 27.30906689384866 25.257944586086438 20.619202140521192 94-95 26.888374245891058 27.35546328978073 25.207019759804943 20.54914270452327 96-97 26.924587284197244 27.369177074851002 25.14529615085193 20.56093949009982 98-99 26.990349589086332 27.465434148950024 25.112540134228155 20.431676127735486 100 26.998452592059934 27.568682752870348 25.029478012314836 20.403386642754874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14739.0 1 10944.5 2 6255.0 3 5351.5 4 5580.5 5 6136.5 6 6845.0 7 7583.5 8 7864.5 9 7909.0 10 8058.0 11 9877.0 12 56413.5 13 69419.0 14 37642.5 15 40329.5 16 45886.5 17 51713.5 18 57583.0 19 63888.5 20 71753.5 21 84254.0 22 103592.5 23 126341.0 24 149664.0 25 174780.0 26 200857.5 27 234719.0 28 282762.5 29 331711.5 30 371907.5 31 419188.0 32 470766.0 33 510902.0 34 551821.5 35 610962.5 36 673174.5 37 716236.0 38 765363.0 39 823120.0 40 869560.0 41 926401.5 42 997605.5 43 1037810.5 44 1061442.0 45 1105330.5 46 1156115.5 47 1204780.5 48 1251141.0 49 1289142.0 50 1312473.5 51 1326649.5 52 1329721.5 53 1319057.0 54 1289440.5 55 1233862.0 56 1159682.5 57 1071079.5 58 963098.5 59 834152.0 60 692840.0 61 559090.5 62 442071.5 63 338553.0 64 253930.0 65 188730.5 66 138407.0 67 102981.0 68 77740.5 69 60137.0 70 47809.0 71 38825.0 72 32578.5 73 26655.0 74 21691.5 75 18719.0 76 14584.0 77 9120.5 78 5643.5 79 3913.5 80 2803.5 81 2010.0 82 1414.0 83 1041.0 84 758.5 85 541.5 86 436.0 87 354.5 88 301.5 89 269.0 90 232.5 91 186.0 92 137.0 93 110.5 94 96.0 95 83.0 96 61.5 97 44.0 98 32.0 99 26.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08129653402552069 2 0.030823232890503573 3 0.021246654666295797 4 0.015518937631983008 5 0.011373105487023546 6 0.011214328936791055 7 0.012766810761286513 8 0.00605409105423513 9 0.001534839985580737 10-11 0.002122901282738107 12-13 0.005560119564622938 14-15 0.052211022268117135 16-17 0.05507488078527353 18-19 0.09174785342925006 20-21 0.11070694964960368 22-23 0.10953229720853182 24-25 0.11391923448532582 26-27 0.11188895285688999 28-29 0.10845026442176225 30-31 0.09253144510771225 32-33 0.09009981164396652 34-35 0.11271517897939609 36-37 0.11375604747536465 38-39 0.10103481146460783 40-41 0.08421037775293545 42-43 0.08395898154840067 44-45 0.0821653945920707 46-47 0.0784753099524082 48-49 0.08338121132394356 50-51 0.08615245018679767 52-53 0.09389427716387445 54-55 0.10439411162461931 56-57 0.10912506476025036 58-59 0.10372519189910279 60-61 0.092341795339379 62-63 0.10009097308267025 64-65 0.09890602956889814 66-67 0.09536296025352499 68-69 0.09842969991820068 70-71 0.10474106778994216 72-73 0.12333556600605822 74-75 0.13426174490724216 76-77 0.12925734326843294 78-79 0.12005271381467719 80-81 0.11766224464173247 82-83 0.10506450150337872 84-85 0.1099424699632991 86-87 0.11554669412520883 88-89 0.11731234816992384 90-91 0.12347817087061887 92-93 0.12864722967263215 94-95 0.14369130780716058 96-97 0.1463272925716685 98-99 0.13773277671371353 100 0.13954106520247245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.401006E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.73088934015086 #Duplication Level Percentage of deduplicated Percentage of total 1 72.00335339013543 31.487706792237883 2 13.693027897187623 11.976165754070212 3 5.0432857740293695 6.6164211628450635 4 2.6470469125604144 4.6303086244547 5 1.5775611795331237 3.449407668474045 6 1.0301903883642727 2.703068512370703 7 0.7096817828449281 2.172451085861877 8 0.5274884669110729 1.8454031819755161 9 0.3919877033546794 1.542777379029303 >10 2.101854491532927 16.65105631036361 >50 0.17114532792366643 5.197668931805941 >100 0.10210274061059992 8.140302470359243 >500 8.852186526403861E-4 0.24127109603114663 >1k 3.4831959001584866E-4 0.282563444449273 >5k 1.3468923090560752E-5 0.04647862011190438 >10k+ 2.693784617812524E-5 3.016948965559672 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 828977 2.4374464496681276 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 152254 0.4476734236869915 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.5027012595684924 5.880612971573705E-6 0.0 0.0 2.9403064857868527E-6 2 0.5027600656982081 1.176122594314741E-5 0.0 5.880612971573705E-6 5.880612971573705E-6 3 0.5027835881500944 1.7641838914721114E-5 0.0 5.880612971573705E-6 5.880612971573705E-6 4 0.502789468763066 2.6462758372081674E-5 0.0 5.880612971573705E-6 1.7641838914721114E-5 5 0.5027924090695518 3.822398431522908E-5 0.0 5.880612971573705E-6 2.352245188629482E-5 6 0.5028012299890091 4.9985210258376495E-5 0.0 5.880612971573705E-6 2.6462758372081674E-5 7 0.5028159315214381 5.58658232299502E-5 0.0 8.820919457360557E-6 2.6462758372081674E-5 8 0.5028218121344097 5.58658232299502E-5 0.0 8.820919457360557E-6 2.9403064857868526E-5 9 0.5028512151992676 6.17464362015239E-5 0.0 1.176122594314741E-5 4.116429080101593E-5 10-11 0.502871797344668 6.615689593020418E-5 0.0 1.6171685671827687E-5 4.704490377258964E-5 12-13 0.5029070810224975 7.644796863045817E-5 0.0 2.2052298643401395E-5 4.704490377258964E-5 14-15 0.5029732379184277 1.014405737596464E-4 0.0 2.6462758372081674E-5 5.1455363501269925E-5 16-17 0.5030291037416577 1.1320179970279382E-4 0.0 2.9403064857868523E-5 5.880612971573705E-5 18-19 0.5030834994116447 1.2790333213172808E-4 0.0 3.2343371343655375E-5 7.056735565888445E-5 20-21 0.5031555369205465 1.4701532428934263E-4 0.0 3.2343371343655375E-5 9.703011403096613E-5 22-23 0.5033672389875231 1.5877655023249E-4 0.0 3.2343371343655375E-5 1.1467195294568726E-4 24-25 0.5044478016210499 1.79358695632998E-4 2.9403064857868527E-6 3.675383107233566E-5 1.1908241267436753E-4 26-27 0.5081643490190844 1.970005345477191E-4 2.9403064857868527E-6 3.96941375581225E-5 1.2937348537462153E-4 28-29 0.5201446277954229 2.1170206697665336E-4 2.9403064857868527E-6 4.410459728680279E-5 1.514257840180229E-4 30-31 0.5478526059642352 2.234632929198008E-4 2.9403064857868527E-6 6.0276282958630475E-5 1.7053777617563744E-4 32-33 0.601793998599238 2.3522451886294818E-4 2.9403064857868527E-6 6.76270491730976E-5 1.79358695632998E-4 34-35 0.6940622862764723 2.4845589804898903E-4 2.9403064857868527E-6 7.350766214467131E-5 1.837691553616783E-4 36-37 0.8338312252315933 2.5580666426345617E-4 2.9403064857868527E-6 8.085842835913845E-5 1.8817961509035857E-4 38-39 1.0272886904639391 2.646275837208167E-4 2.9403064857868527E-6 8.232858160203186E-5 1.9847068779061254E-4 40-41 1.2732306264675803 2.69038043449497E-4 2.9403064857868527E-6 8.673904133071214E-5 2.0876176049086653E-4 42-43 1.5711645319061478 2.866798823642181E-4 2.9403064857868527E-6 8.820919457360557E-5 2.131722202195468E-4 44-45 1.9198422466764244 3.043217212789392E-4 2.9403064857868527E-6 9.114950105939243E-5 2.1758267994822708E-4 46-47 2.328971192641236 3.146127939791932E-4 2.9403064857868527E-6 9.850026727385956E-5 2.293439058913745E-4 48-49 2.7984837427514098 3.3225463289391434E-4 2.9403064857868527E-6 1.0438088024543327E-4 2.3669467210584162E-4 50-51 3.3184798850693005 3.454860120799552E-4 2.9403064857868527E-6 1.1026149321700697E-4 2.4404543832030876E-4 52-53 3.878087836363711 3.572472380231026E-4 2.9403064857868527E-6 1.1467195294568725E-4 2.4845589804898903E-4 54-55 4.475661025002602 3.660681574804631E-4 2.9403064857868527E-6 1.1467195294568725E-4 2.572768175063496E-4 56-57 5.124126508450734 3.807696899093974E-4 2.9403064857868527E-6 1.176122594314741E-4 2.79329116149751E-4 58-59 5.821971499021172 3.984115288241185E-4 2.9403064857868527E-6 1.1908241267436753E-4 2.999112615502589E-4 60-61 6.558059585898995 4.1458321449594623E-4 4.410459728680279E-6 1.2202271916015438E-4 3.219635601936603E-4 62-63 7.322037949947751 4.263444404390936E-4 5.880612971573705E-6 1.2496302564594122E-4 3.3372478613680776E-4 64-65 8.109097719909933 4.48396739082495E-4 5.880612971573705E-6 1.2790333213172808E-4 3.3813524586548806E-4 66-67 8.925485576914596 4.557475052969621E-4 8.820919457360558E-6 1.3084363861751494E-4 3.454860120799552E-4 68-69 9.771198580655254 4.6309827151142923E-4 1.176122594314741E-5 1.3525409834619522E-4 3.631278509946763E-4 70-71 10.640620745744053 4.7191919096878983E-4 1.176122594314741E-5 1.3525409834619522E-4 3.7194877045203684E-4 72-73 11.527183427491748 4.7926995718325697E-4 1.176122594314741E-5 1.3525409834619522E-4 3.837099963951842E-4 74-75 12.426255643183223 4.954416428550846E-4 1.6171685671827687E-5 1.3819440483198208E-4 3.9988168206701196E-4 76-77 13.340458676050556 5.204342479842728E-4 1.7641838914721114E-5 1.411347113177689E-4 4.160533677388396E-4 78-79 14.270730483862716 5.424865466276743E-4 1.7641838914721114E-5 1.455451710464492E-4 4.234041339533068E-4 80-81 15.215900824638357 5.571880790566086E-4 1.7641838914721114E-5 1.4701532428934263E-4 4.248742871962002E-4 82-83 16.169324605719602 5.748299179713296E-4 1.7641838914721114E-5 1.4701532428934263E-4 4.38105666382241E-4 84-85 17.12812620736335 5.983523698576245E-4 1.7641838914721114E-5 1.4701532428934263E-4 4.4545643259670813E-4 86-87 18.090939857206955 6.218748217439193E-4 1.7641838914721114E-5 1.4701532428934263E-4 4.5427735205406873E-4 88 18.81632669862976 6.38046507415747E-4 1.7641838914721114E-5 1.5289593726091632E-4 4.58687811782749E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 120840 0.0 76.77837 8 GTGGTAT 121460 0.0 76.46225 6 AGTGGTA 123035 0.0 75.6447 5 TGGTATC 122715 0.0 75.52343 7 GTATCAA 123035 0.0 75.22487 9 AAGCAGT 129860 0.0 72.827 1 GCAGTGG 135215 0.0 69.731804 3 CAGTGGT 134815 0.0 69.33286 4 AGCAGTG 143640 0.0 65.96562 2 CAACGCA 118855 0.0 41.445328 12-13 ATCAACG 120885 0.0 40.912 10-11 ACGCAGA 122865 0.0 40.05298 14-15 AGAGTAC 121325 0.0 39.69947 18-19 TCAACGC 117900 0.0 39.27713 12-13 GAGTACT 113695 0.0 38.49858 20-21 AGTACTT 122160 0.0 38.2356 20-21 AACGCAG 120740 0.0 38.18181 14-15 TATCAAC 125840 0.0 36.787445 10-11 CGCAGAG 125660 0.0 36.536674 16-17 GTACTTT 120820 0.0 36.218655 22-23 >>END_MODULE