FastQCFastQC Report
Thu 26 May 2016
SRR1660643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34010060
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8068502.372386288057122No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG1558930.45837319898876977TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1175350.075.834658
GTGGTAT1179750.075.567756
AGTGGTA1192450.074.892975
TGGTATC1194050.074.45817
GTATCAA1200900.074.1077359
AAGCAGT1249300.072.272931
GCAGTGG1303800.068.983353
CAGTGGT1313100.068.212074
AGCAGTG1399800.064.6585852
CAACGCA1153100.041.0451712-13
ATCAACG1174550.040.3696110-11
ACGCAGA1201050.039.3849914-15
AGAGTAC1195200.039.05039618-19
TCAACGC1147300.038.6866712-13
GAGTACT1107100.038.23847220-21
AGTACTT1198200.037.67298520-21
AACGCAG1178550.037.56517414-15
TATCAAC1233000.036.1387510-11
CGCAGAG1226850.035.9368916-17
GTACTTT1182250.035.74549522-23
Produced by FastQC (version 0.11.5)