FastQCFastQC Report
Thu 26 May 2016
SRR1660642_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660642_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24626987
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8143553.3067585571876905No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT266270.10812122489852291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1149700.079.057368
AGTGGTA1161450.078.446185
GTGGTAT1166400.078.036336
AAGCAGT1187050.077.887361
TGGTATC1172400.077.417047
GTATCAA1173500.077.260399
GCAGTGG1231500.074.827693
CAGTGGT1243750.073.536294
AGCAGTG1286400.071.9695742
CAACGCA1133550.042.37915812-13
ATCAACG1151450.041.86952210-11
ACGCAGA1169350.041.07761414-15
AGAGTAC1172300.040.2470718-19
TCAACGC1133700.039.95196512-13
AGTACTT1147300.039.61683720-21
GAGTACT1086200.039.37658320-21
AACGCAG1155100.039.12566414-15
TATCAAC1185350.038.17791710-11
CGCAGAG1185850.037.9696916-17
GTACTTT1142600.037.3275922-23
Produced by FastQC (version 0.11.5)