##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660642_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24626987 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23274349395645 34.0 31.0 34.0 30.0 34.0 2 32.400973411810384 34.0 31.0 34.0 30.0 34.0 3 32.42360882392962 34.0 31.0 34.0 30.0 34.0 4 35.80732336440507 37.0 35.0 37.0 35.0 37.0 5 35.80129940378009 37.0 35.0 37.0 35.0 37.0 6 35.8173805427355 37.0 36.0 37.0 35.0 37.0 7 35.76447882154646 37.0 35.0 37.0 35.0 37.0 8 35.79513311961386 37.0 35.0 37.0 35.0 37.0 9 37.4956700143627 39.0 38.0 39.0 35.0 39.0 10-11 37.39443060980217 39.0 37.5 39.0 34.5 39.0 12-13 37.42265070834691 39.0 38.0 39.0 35.0 39.0 14-15 38.822953372249714 41.0 39.0 41.0 35.0 41.0 16-17 38.75760632025347 40.0 38.5 41.0 35.0 41.0 18-19 38.64460374304011 40.0 38.5 41.0 34.0 41.0 20-21 38.60177499586125 40.0 38.5 41.0 34.0 41.0 22-23 38.464536668655406 40.0 38.0 41.0 34.0 41.0 24-25 38.43430662061908 40.0 38.0 41.0 34.0 41.0 26-27 38.37113886485586 40.0 38.0 41.0 34.0 41.0 28-29 38.27187826509187 40.0 38.0 41.0 34.0 41.0 30-31 38.10041890223924 40.0 38.0 41.0 33.0 41.0 32-33 38.08815844991513 40.0 38.0 41.0 33.0 41.0 34-35 38.13955474942996 40.0 38.0 41.0 33.0 41.0 36-37 37.99304149549435 40.0 38.0 41.0 33.0 41.0 38-39 37.68295112593351 40.0 37.0 41.0 33.0 41.0 40-41 37.53051611632394 40.0 37.0 41.0 32.0 41.0 42-43 37.46658797521597 40.0 36.5 41.0 32.0 41.0 44-45 37.56899183403962 40.0 37.0 41.0 32.5 41.0 46-47 37.456604009252125 40.0 36.5 41.0 32.0 41.0 48-49 37.295649869795284 40.0 36.0 41.0 31.5 41.0 50-51 36.16880059261817 38.5 34.5 40.0 29.5 40.5 52-53 36.24921345432959 38.5 35.0 40.0 30.0 40.5 54-55 36.576574531833714 39.0 35.0 41.0 29.5 41.0 56-57 36.27676416526309 39.0 35.0 41.0 28.0 41.0 58-59 36.02626541362936 39.0 35.0 41.0 28.0 41.0 60-61 35.769218134561086 39.0 35.0 41.0 27.5 41.0 62-63 35.49826943913196 38.0 35.0 40.5 27.0 41.0 64-65 35.1921313800994 37.5 34.5 40.0 26.0 41.0 66-67 34.83824679811623 37.0 34.0 40.0 26.0 41.0 68-69 34.49795013494749 36.0 34.0 39.0 26.0 41.0 70-71 34.1649243977755 36.0 34.0 39.0 26.0 41.0 72-73 33.78241997285336 35.0 33.0 38.5 25.5 40.5 74-75 33.3840572945444 35.0 33.0 37.0 25.0 40.0 76-77 32.996594548898734 35.0 33.0 37.0 24.5 39.0 78-79 32.63881953971877 35.0 33.0 36.5 24.0 39.0 80-81 32.29994477196906 35.0 32.5 36.0 23.0 38.0 82-83 31.96875066365203 35.0 32.0 35.5 22.5 37.0 84-85 31.704495478882578 35.0 32.0 35.0 20.5 37.0 86-87 31.438760129284187 35.0 31.5 35.0 20.0 36.0 88-89 31.221472971906795 35.0 31.0 35.0 20.0 36.0 90-91 30.990240503233302 34.0 31.0 35.0 19.5 36.0 92-93 30.817186487327906 34.0 31.0 35.0 19.0 35.0 94-95 30.626636543073662 34.0 30.5 35.0 18.5 35.0 96-97 30.433756817267174 34.0 30.0 35.0 18.0 35.0 98-99 30.287687669628443 34.0 30.0 35.0 17.5 35.0 100 30.15084236654691 34.0 30.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2455959764158564 1101 2 0.24417657487154543 1101 3 0.24608155878581073 1101 4 0.20575333129222884 1101 5 0.2055213108560423 1101 6 0.1803203019761952 1101 7 0.18946260573432738 1101 8 0.1946437106864849 1101 9 0.2702015290091495 1101 10-11 0.3155587535122635 1101 12-13 0.2571042296116346 1101 14-15 0.31511857805356414 1101 16-17 0.2821135211877035 1101 18-19 0.2554979785935245 1101 20-21 0.32207437710959397 1101 22-23 0.2773719940456303 1101 24-25 0.27610280019738553 1101 26-27 0.2730024874151056 1101 28-29 0.23031960000349017 1101 30-31 0.2841621658881124 1101 32-33 0.23739621545268363 1101 34-35 0.23109561589624406 1101 36-37 0.25381161869459845 1101 38-39 0.24143530952724035 1101 40-41 0.18764043802544705 1101 42-43 0.21694003017444885 1101 44-45 0.2589321341418298 1101 46-47 0.2599006530012389 1101 48-49 0.23876749193311042 1101 50-51 0.16688091907570168 1101 52-53 0.2685943162118889 1101 54-55 0.3489416876355875 1101 56-57 0.3046368541808633 1101 58-59 0.26576550789010156 1101 60-61 0.3088447287430611 1101 62-63 0.30217820916103477 1101 64-65 0.23940892321703444 1101 66-67 0.22995281327914796 1101 68-69 0.219008149870497 1101 70-71 0.18394207793123485 1101 72-73 0.2069391575402264 1101 74-75 0.14920004154286204 1101 76-77 0.15771647709956227 1101 78-79 0.1727450310219183 1101 80-81 0.14621686951156931 1101 82-83 0.11905801029689655 1101 84-85 0.03320414729873988 1101 86-87 0.03884822550696043 1101 88-89 0.02944879787496646 1101 90-91 0.005098662820561373 1101 92-93 0.03451409164608421 1101 94-95 -0.018364616253304433 1101 96-97 0.011165756728598097 1101 98-99 -0.007927480805193454 1101 100 -0.11031511530508098 1102 1 0.19274645820729575 1102 2 0.20478301545543331 1102 3 0.1961008001234319 1102 4 0.17579064976079195 1102 5 0.19597250798661037 1102 6 0.20300249764932943 1102 7 0.18926989149473172 1102 8 0.20035225259113076 1102 9 0.26811541398030414 1102 10-11 0.26443474982031034 1102 12-13 0.2318267592309411 1102 14-15 0.21577093434527939 1102 16-17 0.2759537537725478 1102 18-19 0.2393120638130739 1102 20-21 0.3063051716218652 1102 22-23 0.2758377837520456 1102 24-25 0.25872652607564817 1102 26-27 0.24118387606032599 1102 28-29 0.2796975316760424 1102 30-31 0.29678036153768517 1102 32-33 0.2068147566802523 1102 34-35 0.1977599165191819 1102 36-37 0.22748855366774734 1102 38-39 0.23017801113102365 1102 40-41 0.2228332459817537 1102 42-43 0.274424030370632 1102 44-45 0.18882039374122428 1102 46-47 0.2503941573647239 1102 48-49 0.2902539058082354 1102 50-51 0.27106346021637506 1102 52-53 0.3401755655564429 1102 54-55 0.3839161742837973 1102 56-57 0.3861377272819837 1102 58-59 0.34712161405084174 1102 60-61 0.32953769638599795 1102 62-63 0.31636829936876865 1102 64-65 0.28108331466398084 1102 66-67 0.31898578161308677 1102 68-69 0.34670552616824324 1102 70-71 0.27970349716374443 1102 72-73 0.2884460389285266 1102 74-75 0.21455241469819697 1102 76-77 0.28832660186815673 1102 78-79 0.20142339850904278 1102 80-81 0.15379694796758514 1102 82-83 0.12756430124748874 1102 84-85 0.10337725485375415 1102 86-87 0.1465120010199641 1102 88-89 0.16509572636626402 1102 90-91 0.16395427100745152 1102 92-93 0.08503464865938781 1102 94-95 0.019739595269509636 1102 96-97 0.042254170344904196 1102 98-99 -0.03849925806255783 1102 100 -0.09963646256352021 1103 1 0.09771649869532695 1103 2 0.15595313157620438 1103 3 0.12733522414200849 1103 4 0.1543738325928885 1103 5 0.15720055205148498 1103 6 0.146380009005739 1103 7 0.1498925843009289 1103 8 0.15874270530581924 1103 9 0.16484613957810978 1103 10-11 0.2027692467478346 1103 12-13 0.1858511691331728 1103 14-15 0.2012220533546909 1103 16-17 0.23074471321317702 1103 18-19 0.24663514124272723 1103 20-21 0.24498545554478568 1103 22-23 0.23326937882540477 1103 24-25 0.22139557570207558 1103 26-27 0.19193558008229417 1103 28-29 0.18876461590661364 1103 30-31 0.21746639257158762 1103 32-33 0.1594488191668475 1103 34-35 0.1710275591206809 1103 36-37 0.19438438175458117 1103 38-39 0.16835626318549402 1103 40-41 0.10098800718963474 1103 42-43 0.13944119464395044 1103 44-45 0.08563544232409015 1103 46-47 0.12389956838775618 1103 48-49 0.18125401508790873 1103 50-51 0.07827890967634943 1103 52-53 0.19820341547761444 1103 54-55 0.3019057961797529 1103 56-57 0.316776062241118 1103 58-59 0.3007568038172934 1103 60-61 0.3041730439326713 1103 62-63 0.3020875256431097 1103 64-65 0.20661825243170284 1103 66-67 0.22863210010848434 1103 68-69 0.23433016209498447 1103 70-71 0.1632575036101187 1103 72-73 0.20080224166065364 1103 74-75 0.19698342812718295 1103 76-77 0.2067782767714732 1103 78-79 0.18975326082087207 1103 80-81 0.12483030156361252 1103 82-83 0.13688839195513935 1103 84-85 0.137625306773419 1103 86-87 0.1041351711474725 1103 88-89 0.11071888259141716 1103 90-91 0.10297506035191262 1103 92-93 0.03579323779943522 1103 94-95 0.025328831196528512 1103 96-97 0.09272602169996702 1103 98-99 0.03433489315787597 1103 100 -0.036214166529347835 1104 1 0.10662720377892754 1104 2 0.13458600466635318 1104 3 0.10543615464971623 1104 4 0.11935269772205714 1104 5 0.1287467705880161 1104 6 0.09624759713503295 1104 7 0.11977329968069483 1104 8 0.0930755101364582 1104 9 0.10961711325800394 1104 10-11 0.11921944582711319 1104 12-13 0.14254633612923584 1104 14-15 0.12677055028782291 1104 16-17 0.13130954030145148 1104 18-19 0.09506517600269149 1104 20-21 0.10964410375952838 1104 22-23 0.11585555474930942 1104 24-25 0.1448561462694542 1104 26-27 0.13487309186885454 1104 28-29 0.12649249302500465 1104 30-31 0.1431548061167902 1104 32-33 0.11988389221809115 1104 34-35 0.13290424288455682 1104 36-37 0.12932286414316962 1104 38-39 0.04926355191287968 1104 40-41 0.10993770653714563 1104 42-43 0.07664853698488372 1104 44-45 0.021533125004765452 1104 46-47 0.06825970751540922 1104 48-49 0.09441812981145148 1104 50-51 0.058477586092514855 1104 52-53 0.15870921267637783 1104 54-55 0.1988719684906215 1104 56-57 0.2259744446650913 1104 58-59 0.22309646737068078 1104 60-61 0.21868830712210752 1104 62-63 0.19239767061045399 1104 64-65 0.19043992485616457 1104 66-67 0.16235149622149692 1104 68-69 0.20105790303705362 1104 70-71 0.16580211398260047 1104 72-73 0.11995982374826752 1104 74-75 0.14823864322730884 1104 76-77 0.14557519383475892 1104 78-79 0.09526175229645162 1104 80-81 0.054181738963912096 1104 82-83 0.03151538329378667 1104 84-85 0.05442289781357701 1104 86-87 0.07050616837567603 1104 88-89 0.023349267432276832 1104 90-91 0.04608327093056985 1104 92-93 -0.03159905168305244 1104 94-95 -0.023517118534297055 1104 96-97 -0.016673292472617618 1104 98-99 -0.08325080373116833 1104 100 -0.1599667039576822 1105 1 0.061803921310456644 1105 2 0.06556342823804329 1105 3 0.05324587006184345 1105 4 0.052870798868092095 1105 5 0.07191228622773593 1105 6 0.03443901739827737 1105 7 0.07496564491728464 1105 8 0.09329907800037773 1105 9 0.04062758973023506 1105 10-11 0.07237154052839401 1105 12-13 0.05724562559725399 1105 14-15 0.03984869931466761 1105 16-17 -0.010528707271461712 1105 18-19 0.05756757524820699 1105 20-21 0.0748827180543401 1105 22-23 0.07871429458226231 1105 24-25 0.10846000579751092 1105 26-27 0.047296087022651534 1105 28-29 0.03485058247742501 1105 30-31 0.024097293037002032 1105 32-33 0.012571909092990552 1105 34-35 0.017434077490733557 1105 36-37 0.019158182883295183 1105 38-39 0.030171985805068857 1105 40-41 -0.004901519747427585 1105 42-43 0.050867818562657874 1105 44-45 0.06427326253956522 1105 46-47 0.10225721210035488 1105 48-49 0.08407464238373308 1105 50-51 0.06040059745511428 1105 52-53 0.11629738153585123 1105 54-55 0.17530711161345636 1105 56-57 0.22267960953772814 1105 58-59 0.1550763475449486 1105 60-61 0.2423399837375726 1105 62-63 0.19496975100815916 1105 64-65 0.20991009760734158 1105 66-67 0.2064853336451975 1105 68-69 0.14945933802938782 1105 70-71 0.10202451093272913 1105 72-73 0.12393928017890943 1105 74-75 0.11062768169477266 1105 76-77 0.14692140478761928 1105 78-79 0.10972865801844023 1105 80-81 0.10348928545277403 1105 82-83 0.0981572102913475 1105 84-85 0.04325913086384148 1105 86-87 0.11431105078686699 1105 88-89 0.1381839714592097 1105 90-91 0.05765112894338742 1105 92-93 0.023467045434919953 1105 94-95 0.036310921586977685 1105 96-97 0.09372022073026187 1105 98-99 0.005597866621322112 1105 100 -0.02266998426528488 1106 1 -0.03242672921420109 1106 2 -0.02807098706689004 1106 3 7.473003599685057E-4 1106 4 0.02852314137915357 1106 5 0.03280818276702746 1106 6 0.01666402321482252 1106 7 0.057196227158613056 1106 8 0.03589561126580776 1106 9 0.016093729629581333 1106 10-11 0.06471799190604344 1106 12-13 0.03612520709602762 1106 14-15 0.0035921319082419245 1106 16-17 0.035129923530412555 1106 18-19 -0.018967500203849852 1106 20-21 0.039325226142310044 1106 22-23 -0.006975326148641159 1106 24-25 0.024585505937153584 1106 26-27 0.023481077871160494 1106 28-29 -0.008486874995348614 1106 30-31 0.025452795701689013 1106 32-33 0.04620567018370991 1106 34-35 0.024490483061462953 1106 36-37 -0.08764206434826605 1106 38-39 -0.0893576131754017 1106 40-41 -0.013802313430325341 1106 42-43 0.04330343341300846 1106 44-45 0.04902631769631682 1106 46-47 0.013687181014773842 1106 48-49 -0.034485810104705195 1106 50-51 -0.20649573366060991 1106 52-53 -0.05142911743674716 1106 54-55 0.005240949203120238 1106 56-57 -0.002404179884798907 1106 58-59 0.03429454278691679 1106 60-61 0.08939788566693352 1106 62-63 0.043983115629202985 1106 64-65 0.027262115118006136 1106 66-67 0.08741328463180054 1106 68-69 0.06223491783631374 1106 70-71 0.057181089794546835 1106 72-73 -0.051467990687655174 1106 74-75 -0.0978285537245327 1106 76-77 9.760155998890241E-4 1106 78-79 -0.028100541530569956 1106 80-81 0.002022229664312647 1106 82-83 0.030880481256446046 1106 84-85 0.015684642462122866 1106 86-87 -0.011109089091043245 1106 88-89 -0.01113194944043272 1106 90-91 -0.029466040350211387 1106 92-93 -0.053839298819859494 1106 94-95 -0.043625616577838144 1106 96-97 -0.05679778643608202 1106 98-99 -0.1043490167102874 1106 100 -0.16503851941757475 1107 1 -0.09977969603870918 1107 2 -0.06320200147229826 1107 3 -0.042693100787602134 1107 4 -0.005349159791173008 1107 5 -0.027475597301815924 1107 6 -0.051083191269114536 1107 7 -0.04951451639130511 1107 8 -0.08031794727681785 1107 9 -0.07243069409111769 1107 10-11 -0.009409997143748683 1107 12-13 -0.04294076749096121 1107 14-15 -0.09043646490325585 1107 16-17 -0.10937201156732357 1107 18-19 -0.08674172289687476 1107 20-21 -0.03863697672922228 1107 22-23 -0.13027244668857207 1107 24-25 -0.08037375580305195 1107 26-27 -0.11301124562806564 1107 28-29 -0.12299434384679131 1107 30-31 -0.06989738246288368 1107 32-33 -0.1436123396458484 1107 34-35 -0.11477448767869447 1107 36-37 -0.0917796765001313 1107 38-39 -0.09860431470371367 1107 40-41 -0.12436978172876678 1107 42-43 -0.08280023221055188 1107 44-45 -0.1251947299304419 1107 46-47 -0.14697588344373003 1107 48-49 -0.19072681860451013 1107 50-51 -0.3172353024704435 1107 52-53 -0.107357190831884 1107 54-55 -0.053392369682441654 1107 56-57 0.014969557959261692 1107 58-59 0.015166918101527926 1107 60-61 0.07576223054466169 1107 62-63 0.08372815570081826 1107 64-65 0.05329753837122553 1107 66-67 0.06958246313376293 1107 68-69 0.04838237282137925 1107 70-71 0.0250430953804468 1107 72-73 0.03539361446767231 1107 74-75 0.025054381486206978 1107 76-77 -0.004068157366276637 1107 78-79 0.02941020243459036 1107 80-81 0.03015379308644839 1107 82-83 0.03392106169883391 1107 84-85 -0.019044571047505343 1107 86-87 -7.428085762519743E-4 1107 88-89 0.004973794874342019 1107 90-91 0.048158159632716746 1107 92-93 -0.09471257261032662 1107 94-95 -0.06976526265774652 1107 96-97 -0.03950851634537145 1107 98-99 -0.1479546701579828 1107 100 -0.0920271616055075 1108 1 -0.033560301631609946 1108 2 -0.01519302773985487 1108 3 -0.04370994406379225 1108 4 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2316 52-53 -1.3544086677845186 2316 54-55 -1.3886822302071948 2316 56-57 -1.52027461999419 2316 58-59 -1.6512681963850184 2316 60-61 -1.2195665203735686 2316 62-63 -1.0364183788276975 2316 64-65 -1.1464623502001743 2316 66-67 -1.2326613687278112 2316 68-69 -1.1388789744225747 2316 70-71 -0.9349667565736226 2316 72-73 -0.9993516761880912 2316 74-75 -1.0497087443226363 2316 76-77 -1.4662309358085324 2316 78-79 -1.2905863269160456 2316 80-81 -1.1390297122626052 2316 82-83 -1.4073883588190057 2316 84-85 -1.0659839458066251 2316 86-87 -1.0477911012014012 2316 88-89 -1.388822461344187 2316 90-91 -1.6323069728205866 2316 92-93 -1.2048469697182433 2316 94-95 -1.0397408348034283 2316 96-97 -1.6820918928763362 2316 98-99 -1.6830589627214394 2316 100 -1.3237844425800311 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 413.0 3 1043.0 4 1738.0 5 5813.0 6 5232.0 7 5828.0 8 12544.0 9 17432.0 10 19773.0 11 19712.0 12 21512.0 13 25377.0 14 31155.0 15 39309.0 16 51134.0 17 65906.0 18 75189.0 19 83354.0 20 96820.0 21 116543.0 22 148498.0 23 209024.0 24 292217.0 25 357631.0 26 362464.0 27 328331.0 28 315405.0 29 343310.0 30 403077.0 31 492616.0 32 617819.0 33 804816.0 34 1119323.0 35 1708979.0 36 2987894.0 37 5533073.0 38 6244620.0 39 1658775.0 40 3288.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.610551047670594 30.41703342069865 28.25526300079121 24.717152530839545 2 22.31020999800604 31.90647478653866 25.95440761200275 19.828907603452556 3 25.102257399877914 24.757679103262205 26.955355217290123 23.18470827956976 4 20.46514738998704 27.548352303816458 24.13083466375872 27.85566564243778 5 23.102727059410807 29.86270445716892 26.152856067140064 20.88171241628021 6 27.73152843744562 27.427115713912386 24.943950315412096 19.897405533229897 7 22.49267618656824 25.442300013677833 29.896790030321903 22.168233769432025 8 26.352138879321107 26.75602971330573 25.067891101025257 21.823940306347907 9 25.3072002944458 24.97486864205881 27.237645109329733 22.480285954165666 10-11 22.100163101029484 28.39769582742201 27.551213828836257 21.950927242712247 12-13 21.901921206446893 26.51361584837353 27.559017115869167 24.025445829310417 14-15 21.94484221159638 30.295637981089136 25.748210779113784 22.011309028200703 16-17 24.03899666560084 26.168378422411127 25.83638895175055 23.956235960237482 18-19 21.97178032972988 28.168174298946063 25.913146618642237 23.946898752681818 20-21 23.933451549184586 28.32163167144124 25.87172197973338 21.8731947996408 22-23 23.91476011770542 26.315585146729532 27.774662748572858 21.99499198699219 24-25 22.0834407849934 27.956398398009323 25.81154028694822 24.148620530049055 26-27 22.137494838141432 26.106639871752563 29.764703400155568 21.991161889950437 28-29 22.184585009536427 26.153493959530554 29.711109962493527 21.950811068439492 30-31 22.097101346161892 26.054432041826175 29.768181950553746 22.080284661458187 32-33 22.185508662068838 26.25449266372433 29.526685946484736 22.033312727722098 34-35 22.179540246931168 26.36939522889843 29.430950042839843 22.02011448133056 36-37 22.11325319234295 26.313336584374508 29.54570571792366 22.027704505358884 38-39 22.134201149537656 26.307446549011154 29.60584632505614 21.952505976395052 40-41 22.166249042114686 26.365868612974342 29.53269014593751 21.935192198973457 42-43 22.16657657574728 26.377509665088354 29.50630521624899 21.949608542915374 44-45 22.247444227276596 26.4564026429318 29.455684245558196 21.840468884233417 46-47 22.294550339467424 26.55689782539769 29.327708771875926 21.820843063258963 48-49 22.348468330495052 26.5925015513579 29.229901426338916 21.829128691808126 50-51 22.47320729621769 26.65058318357985 29.132715030764178 21.74349448943828 52-53 22.634844632430802 26.743990335567673 28.876052078420354 21.745112953581167 54-55 22.864564789185778 26.93547294153839 28.342068403827366 21.857893865448467 56-57 23.256366999329256 27.309882314681193 27.588408300981726 21.845342385007825 58-59 23.454129423586856 27.599849176120898 27.17004949294698 21.775971907345262 60-61 23.59881016168354 27.71938903933838 26.98688070186577 21.694920097112306 62-63 23.74536545890282 27.83192159131508 26.851670763891388 21.57104218589071 64-65 23.935600918311067 27.86493489727979 26.762509622645954 21.43695456176319 66-67 24.077891070097646 27.728243916383434 26.86984997676902 21.324015036749902 68-69 24.22820304885368 27.63142079868876 27.004193829152545 21.136182323305018 70-71 24.3902303195857 27.559140833963397 27.05765306537239 20.99297578107851 72-73 24.557386777529032 27.508935082716423 27.04784427954064 20.885833860213907 74-75 24.796985200364695 27.596275292795198 26.908296422090654 20.69844308474946 76-77 25.0892377887996 27.63658147479594 26.64953319011863 20.62464754628583 78-79 25.333062486452828 27.709430815605863 26.401257051517028 20.556249646424277 80-81 25.579454466869084 27.828582215995823 26.13773852624286 20.454224790892233 82-83 25.780593193999522 27.89494933169478 25.908102351708205 20.416355122597484 84-85 25.943098924009817 27.873199369991262 25.802387840341172 20.381313865657745 86-87 26.13414604474077 27.845277761420302 25.70476745670617 20.315808737132766 88-89 26.29772345138518 27.799504242260735 25.62866759863137 20.274104707722714 90-91 26.47147175198169 27.707375216923914 25.58278275192564 20.23837027916876 92-93 26.581707220978913 27.729216157234184 25.546986564695867 20.142090057091036 94-95 26.71301041140179 27.68385460693153 25.489067737366415 20.11406724430027 96-97 26.746212074846483 27.66702109978426 25.458833780166856 20.1279330452024 98-99 26.85158790137963 27.72085065830927 25.41858634865055 20.008975091660545 100 26.871904388032757 27.76969793674936 25.322738209734947 20.035659465482937 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11072.0 1 8850.5 2 5763.5 3 4896.0 4 5080.5 5 5562.0 6 6124.0 7 6799.5 8 7027.0 9 7029.0 10 7321.5 11 9163.0 12 56378.0 13 70275.0 14 39264.0 15 42674.0 16 48473.0 17 53927.5 18 59180.5 19 64187.5 20 70278.0 21 79248.0 22 92062.5 23 107785.5 24 124286.5 25 143264.0 26 165289.0 27 193170.5 28 227305.0 29 260377.0 30 291529.0 31 324959.0 32 359653.0 33 391739.5 34 424753.0 35 465143.5 36 509275.0 37 550304.0 38 591847.0 39 626168.0 40 650064.5 41 682943.0 42 723091.0 43 750202.0 44 774463.0 45 806806.5 46 840839.0 47 872375.0 48 898861.5 49 921194.0 50 935113.0 51 941682.0 52 940877.0 53 922437.0 54 886635.5 55 840276.0 56 785465.5 57 720335.5 58 642372.5 59 556449.0 60 463715.0 61 374608.0 62 297000.0 63 228145.5 64 170469.0 65 125020.5 66 91136.5 67 66768.5 68 48722.0 69 36197.5 70 27621.5 71 21270.0 72 16863.5 73 13230.5 74 10297.5 75 8371.5 76 6456.0 77 4401.0 78 3056.0 79 2241.0 80 1628.5 81 1179.5 82 843.0 83 624.0 84 492.0 85 407.0 86 317.5 87 266.0 88 239.5 89 201.0 90 158.5 91 128.0 92 106.5 93 81.0 94 60.5 95 58.0 96 54.0 97 46.0 98 35.0 99 22.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08263292622845012 2 0.031095968012652138 3 0.021508924335729745 4 0.015876891476817687 5 0.011698548425757483 6 0.011580791430149373 7 0.013006057135613057 8 0.0061761513903426345 9 0.001555204459238152 10-11 0.002164292367555966 12-13 0.005761971612686522 14-15 0.05360379651802309 16-17 0.05626754097039966 18-19 0.09388277989508013 20-21 0.11335938091005611 22-23 0.11211278099103232 24-25 0.11660581946139005 26-27 0.11486991892268429 28-29 0.11112808887258518 30-31 0.09520247036310207 32-33 0.09277423990194172 34-35 0.1152840987003404 36-37 0.1165936377032237 38-39 0.10402409356857174 40-41 0.0867077243350963 42-43 0.0864437862414919 44-45 0.08457388636295621 46-47 0.08085845012221755 48-49 0.08577581983537004 50-51 0.08863244212538057 52-53 0.09657291815681715 54-55 0.10698020021694087 56-57 0.11161332890621171 58-59 0.10601378073574327 60-61 0.09464413978047741 62-63 0.1022110418948124 64-65 0.10097053285487179 66-67 0.09771394283839918 68-69 0.10105986574809171 70-71 0.10714668424521441 72-73 0.12573198662101864 74-75 0.13661638754265798 76-77 0.13174774486217092 78-79 0.12207542887808402 80-81 0.12004107526430252 82-83 0.10720353244999074 84-85 0.11225693179600087 86-87 0.1177468441429721 88-89 0.1197426221892268 90-91 0.12625783251519967 92-93 0.13088487032538737 94-95 0.14604303807039 96-97 0.14839817798255223 98-99 0.14001103748501595 100 0.14179160447033168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4626987E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.79065541419026 #Duplication Level Percentage of deduplicated Percentage of total 1 75.94135381099706 36.29287071668465 2 11.743013052313694 11.224125806149248 3 4.30441948307044 6.1713308482063916 4 2.2040315993689075 4.213284587497044 5 1.2760744351082587 3.049221680555813 6 0.8838658033806555 2.5344315625050804 7 0.6262051299131095 2.094872750759296 8 0.4601979244642886 1.75945283443187 9 0.3505981339319654 1.5079783146840617 >10 1.9771352109502376 17.840890329572982 >50 0.18540278775640048 6.009928456701636 >100 0.04668874245159176 3.259987160481878 >500 5.631818162600535E-4 0.1763717476082068 >1k 4.083254935386006E-4 0.3423275732038835 >5k 1.695159343886008E-5 0.05893679459464925 >10k+ 2.542739015746465E-5 3.4639888363634888 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 814355 3.3067585571876905 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 26627 0.10812122489852291 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00853129130250485 1.2181758166356282E-5 0.0 4.060586055452094E-6 1.2181758166356282E-5 2 0.008580018335170275 2.030293027726047E-5 0.0 4.060586055452094E-6 1.2181758166356282E-5 3 0.0086287453678357 2.842410238816466E-5 0.0 4.060586055452094E-6 2.030293027726047E-5 4 0.008653108884168412 3.6545274499068844E-5 0.0 4.060586055452094E-6 3.248468844361675E-5 5 0.00866529064233477 5.278761872087722E-5 0.0 8.121172110904188E-6 4.060586055452094E-5 6 0.008693714744722933 6.49693768872335E-5 0.0 8.121172110904188E-6 4.060586055452094E-5 7 0.008701835916833838 8.121172110904188E-5 0.0 8.121172110904188E-6 4.060586055452094E-5 8 0.008734320605277455 8.121172110904188E-5 0.0 1.2181758166356282E-5 4.466644660997303E-5 9 0.00876680529372107 8.121172110904188E-5 0.0 1.2181758166356282E-5 4.466644660997303E-5 10-11 0.008797259689136961 9.339347927539817E-5 0.0 1.2181758166356282E-5 4.6696739637699084E-5 12-13 0.008860198772996469 1.035449444140284E-4 0.0 1.2181758166356282E-5 4.872703266542513E-5 14-15 0.008908925805661895 1.2384787469128886E-4 0.0 1.2181758166356282E-5 5.4817911748603266E-5 16-17 0.008969834596493677 1.4821139102400143E-4 0.0 1.421205119408233E-5 6.090879083178141E-5 18-19 0.00904495543851954 1.6445373524580982E-4 0.0 2.030293027726047E-5 6.699966991495955E-5 20-21 0.009118045987517677 1.9084754460624843E-4 0.0 2.030293027726047E-5 7.309054899813769E-5 22-23 0.009274378550652582 2.1724135396668703E-4 0.0 2.842410238816466E-5 8.324201413676793E-5 24-25 0.009792103272722726 2.456654563548517E-4 0.0 2.842410238816466E-5 9.339347927539817E-5 26-27 0.01205994058469272 2.781501447984685E-4 4.060586055452094E-6 2.842410238816466E-5 9.745406533085026E-5 28-29 0.01935275314028468 2.903319029648247E-4 4.060586055452094E-6 2.842410238816466E-5 1.0151465138630235E-4 30-31 0.037737056506344034 3.1672571232526335E-4 4.060586055452094E-6 3.248468844361675E-5 1.076055304694805E-4 32-33 0.07740898226811099 3.4108922865797595E-4 4.060586055452094E-6 4.060586055452094E-5 1.1166611652493259E-4 34-35 0.14769163600890356 3.5327098682433214E-4 4.060586055452094E-6 4.6696739637699084E-5 1.1978728863583677E-4 36-37 0.2572015813383911 3.674830380184145E-4 4.060586055452094E-6 4.872703266542513E-5 1.2587816771901492E-4 38-39 0.41342044806374406 3.735739171015927E-4 6.090879083178141E-6 4.872703266542513E-5 1.2790846074674098E-4 40-41 0.6222320253793125 3.979374334343052E-4 8.121172110904188E-6 4.872703266542513E-5 1.3196904680219304E-4 42-43 0.8894774663258644 4.2027065673929173E-4 8.121172110904188E-6 4.872703266542513E-5 1.4009021891309723E-4 44-45 1.2111794268620844 4.3448270793337406E-4 8.121172110904188E-6 4.872703266542513E-5 1.4618109799627538E-4 46-47 1.5947261433158673 4.4666446609973036E-4 8.121172110904188E-6 4.872703266542513E-5 1.5227197707945353E-4 48-49 2.0398597684726925 4.7305827546016894E-4 8.121172110904188E-6 4.872703266542513E-5 1.6445373524580982E-4 50-51 2.5455022167348362 4.974217917928815E-4 8.121172110904188E-6 4.872703266542513E-5 1.72574907356714E-4 52-53 3.1008360868505758 5.096035499592378E-4 8.121172110904188E-6 5.278761872087722E-5 1.8678695855079634E-4 54-55 3.7067790712684423 5.217853081255941E-4 8.121172110904188E-6 5.278761872087722E-5 1.9084754460624843E-4 56-57 4.364790950675371 5.481791174860327E-4 8.121172110904188E-6 5.278761872087722E-5 1.9490813066170052E-4 58-59 5.071674013552693 5.887849780405537E-4 8.121172110904188E-6 5.278761872087722E-5 2.0505959580033073E-4 60-61 5.818643181969438 6.131484943732663E-4 8.121172110904188E-6 5.278761872087722E-5 2.0912018185578285E-4 62-63 6.603241801362058 6.314211316228006E-4 8.121172110904188E-6 5.278761872087722E-5 2.1115047488350888E-4 64-65 7.419671354843368 6.578149409832393E-4 8.121172110904188E-6 5.8878497804055363E-5 2.1521106093896097E-4 66-67 8.265899519092612 6.639058200664173E-4 8.121172110904188E-6 7.106025597041166E-5 2.334836981884954E-4 68-69 9.137544921755957 6.720269921773216E-4 8.121172110904188E-6 7.715113505358979E-5 2.375442842439475E-4 70-71 10.030618037033925 6.862390433714039E-4 8.121172110904188E-6 7.715113505358979E-5 2.456654563548517E-4 72-73 10.944966592949434 7.146631457595686E-4 8.121172110904188E-6 7.715113505358979E-5 2.6190780057666006E-4 74-75 11.877311666262706 7.34966076036829E-4 8.121172110904188E-6 7.715113505358979E-5 2.639380936043861E-4 76-77 12.821385742397151 7.572992993418156E-4 8.121172110904188E-6 8.121172110904188E-5 2.659683866321122E-4 78-79 13.782138675754368 7.796325226468021E-4 8.121172110904188E-6 8.121172110904188E-5 2.761198517707424E-4 80-81 14.754368855597317 8.100869180626927E-4 8.121172110904188E-6 8.121172110904188E-5 2.8424102388164657E-4 82-83 15.73426542191296 8.588139507281179E-4 8.121172110904188E-6 8.527230716449397E-5 2.8424102388164657E-4 84-85 16.720129019437092 8.770865879776523E-4 8.121172110904188E-6 8.527230716449397E-5 2.903319029648247E-4 86-87 17.712144404835232 9.116015694489951E-4 8.121172110904188E-6 8.527230716449397E-5 2.9642278204800287E-4 88 18.457154340480223 9.298742066985295E-4 8.121172110904188E-6 8.527230716449397E-5 2.9642278204800287E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 114970 0.0 79.05736 8 AGTGGTA 116145 0.0 78.44618 5 GTGGTAT 116640 0.0 78.03633 6 AAGCAGT 118705 0.0 77.88736 1 TGGTATC 117240 0.0 77.41704 7 GTATCAA 117350 0.0 77.26039 9 GCAGTGG 123150 0.0 74.82769 3 CAGTGGT 124375 0.0 73.53629 4 AGCAGTG 128640 0.0 71.969574 2 CAACGCA 113355 0.0 42.379158 12-13 ATCAACG 115145 0.0 41.869522 10-11 ACGCAGA 116935 0.0 41.077614 14-15 AGAGTAC 117230 0.0 40.24707 18-19 TCAACGC 113370 0.0 39.951965 12-13 AGTACTT 114730 0.0 39.616837 20-21 GAGTACT 108620 0.0 39.376583 20-21 AACGCAG 115510 0.0 39.125664 14-15 TATCAAC 118535 0.0 38.177917 10-11 CGCAGAG 118585 0.0 37.96969 16-17 GTACTTT 114260 0.0 37.32759 22-23 >>END_MODULE