FastQCFastQC Report
Thu 26 May 2016
SRR1660642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24626987
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8297353.3692103707205434No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT276600.11231581029380493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1146700.079.452628
AGTGGTA1157600.078.8668755
GTGGTAT1167800.078.137786
TGGTATC1172950.077.542317
AAGCAGT1190600.077.522531
GTATCAA1175000.077.363049
GCAGTGG1230300.074.7909243
CAGTGGT1245450.073.598174
AGCAGTG1289300.071.7729642
CAACGCA1136950.042.26790212-13
ATCAACG1152450.041.7952410-11
ACGCAGA1174050.040.9222314-15
AGAGTAC1190200.040.0521118-19
TCAACGC1133850.039.96723612-13
AGTACTT1161000.039.61856520-21
GAGTACT1098400.039.32407820-21
AACGCAG1158050.039.04884714-15
TATCAAC1192650.038.11497510-11
CGCAGAG1186900.038.0185216-17
GTACTTT1153900.037.34206422-23
Produced by FastQC (version 0.11.5)