FastQCFastQC Report
Thu 26 May 2016
SRR1660641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28382694
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8808473.1034650903821888No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC571420.2013269071639218Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1226000.080.171818
AGTGGTA1245350.079.179235
GTGGTAT1246000.078.9749456
GTATCAA1244350.078.785599
TGGTATC1252950.078.32337
AAGCAGT1322800.075.90571
GCAGTGG1342450.074.4342653
CAGTGGT1362450.072.730894
AGCAGTG1420350.070.701512
CAACGCA1202100.042.9222612-13
ATCAACG1219200.042.44766210-11
ACGCAGA1238300.041.63791314-15
AGAGTAC1240100.040.87703318-19
TCAACGC1202150.040.84219412-13
AACGCAG1221200.040.04945414-15
GAGTACT1162000.039.87488620-21
AGTACTT1243100.039.43997620-21
CGCAGAG1257100.038.76162716-17
TATCAAC1265900.038.711710-11
GTACTTT1232900.037.47339222-23
Produced by FastQC (version 0.11.5)